How are alleles named?
IMPC uses standard rules to name alleles. The gene symbol is followed by a superscript that indicates the serial number and the laboratory code. As part of the superscript, “tm” indicates targeted mutation, and “em” an endonuclease-mediated allele. IMPC alleles carry KOMP, EUCOMM or IMPC as part of the laboratory name. Examples:
- Cib2<tm1a(EUCOMM)Wtsi> : targeted, KO first allele (reporter-tagged insertion with conditional potential).
- Nxn<tm1b(EUCOMM)Wtsi> : targeted, reporter-tagged deletion allele (post-Cre).
- Nxn<em1(IMPC)J> : endonuclease-mediated allele; indel.
More information on IMPC allele design can be found here.