Understanding the data

Embryo Viewer

Up to one third of homozygous knockout lines are embryonic lethal, which means no homozygous mice or less than expected are observed past the weaning stage (IMPC Viability Primary Screen procedure). Early death may occur during embryonic development or soon after birth, during the pre-weaning stage. For this reason, the IMPC established a systematic phenotyping pipeline to morphologically evaluate mutant embryos to ascertain the primary perturbations that cause early death and thus gain insight into gene function.

As determined in IMPReSS (see interactive diagram here), all embryonic lethal lines undergo gross morphology assessment at E12.5 (embryonic day 12.5) to determine whether defects occur earlier or later in development. A comprehensive imaging platform is then used to assess dysmorphology. Embryo gross morphology, as well as 2D and 3D imaging are actively being implemented by the IMPC for embryonic lethal lines.

Read more in our paper on High-throughput discovery of novel developmental phenotypes, Nature 2016

Images acquired by micro-CT and OPT

GeneOPT E9.5MicroCT E14.5-E15.5MicroCT E18.5
1700003F12Rik 
4932438A13Rik 
Abca1 
Acan  
Acat2 
Ace 
Actg1 
Acvr2a 
Acvr2b 
Adam23 
Aebp1 
Ahnak 
Akap10 
Aldh1a3 
Ampd1 
Ampd2 
Anapc15 
Anks3 
Ano6 
Ap2b1 
Ap4e1 
Apbb2 
Arhgap33 
Arhgef18  
Arih2 
Arl15  
Atf4 
Atg13  
Atg3 
Atp1a3  
Atp2a1  
Axin2 
B3galnt2 
BC053393 
Baiap2 
Barx2 
Bbox1  
Bcl11b 
Bcl7a 
Bclaf1 
Best2 
Bicdl1 
Bloc1s2 
Bmi1 
Bscl2 
Cacna1s  
Casq2 
Casz1 
Cbx2 
Cbx4 
Ccdc114 
Ccnt2 
Cdc34 
Cdk19 
Cdk4 
Cdkn1c 
Cecr2 
Cep164 
Cep97 
Cgn  
Chic2 
Chpt1 
Chrnb2 
Chst14 
Chtf18 
Ckap4 
Clcf1 
Clcn3 
Cldn1 
Cnnm2 
Cnp 
Cnr1 
Commd9 
Coq2 
Cox7c 
Cps1  
Creb3 
Crispld2 
Cst3 
Ctdspl2 
Ctnnbip1 
D1Ertd622e 
Daam1 
Dbn1   
Dcc 
Depdc5  
Dlg5 
Dnal1 
Dnm1 
Dok7 
Dpm2 
Dpy19l1 
Dsc3  
Dst 
Ecm1 
Egr1 
Elmod2 
Emc8 
Epas1 
Epb41 
Ethe1 
Ewsr1 
Exoc6 
Eya1 
Eya4 
F10 
F2 
F7 
Fadd 
Fam13b 
Fam20c 
Fam49b 
Fbxl16 
Fbxl20 
Fbxl21  
Fbxo11  
Fbxo30 
Fermt3 
Fgf10  
Fgf15 
Fgf3 
Fgf8 
Fgf9  
Flnb 
Flywch1 
Fmc1  
Fmnl3 
Fndc3b 
Foxj3 
Foxp1 
Frmd6 
Frrs1l 
Fscn1 
Furin 
Fus 
Fzd3  
Fzd4 
Fzd5 
Fzr1 
Gabarapl2 
Gabpa 
Gata2 
Gck  
Gdpd5 
Gfi1b 
Gfod2 
Gfpt1 
Ggnbp2 
Gjb3 
Gjc1 
Gm572 
Gna11 
Gnao1  
Got1 
Gramd1b 
Grcc10 
Gyg  
Hap1 
Hipk2 
Hmga1 
Hmgn2 
Hnrnpab 
Hoxb13 
Hpse2 
Inpp5e 
Ints12 
Ippk 
Irx1  
Itpr1 
Jag2  
Jup 
Kcnj10  
Kcnj13 
Kctd15 
Kdm8 
Kif1b 
Kif26b 
Kif3c 
Kif5a 
Klf7  
Klhdc2  
Krt5 
L3mbtl3 
Lama5  
Laptm4b 
Ldb3  
Lefty1  
Lgi4 
Lhx6 
Lifr 
Lpar1 
Lpgat1 
Lrrc8a 
Lum 
Map3k12 
Map3k7 
Mbd6 
Mboat7 
Memo1 
Mfsd2a 
Micu1 
Mllt3 
Mmachc 
Mob2 
Mrpl23 
Myb 
Myo18a 
Myo1h 
Nabp2 
Nalcn 
Ncbp3  
Ncoa3 
Ndel1  
Nedd4l 
Nek7 
Nemf 
Ngfr 
Nhlh1 
Notch1 
Npc1 
Nr1d1  
Nr2f1 
Nras  
Nrxn1 
Nsd3 
Ntrk1  
Ntrk2  
Nxn  
Otub1 
Otud4 
Otud6b  
Pard3 
Patl1 
Pax7 
Pbx3 
Pccb 
Pcdh9 
Pcsk5 
Pcx  
Pdgfrb  
Pdpn 
Pdx1  
Pex26 
Pfdn5 
Piezo1 
Pip5k1c 
Pitx2 
Pkd1  
Plpp3 
Pparg 
Pptc7 
Prdm12 
Prkab2  
Prkg1 
Psen1  
Pskh1 
Psph 
Ptk7  
Ptpn12 
Ptprs 
Pts 
Pttg1ip 
Rab23  
Rab34 
Rad1 
Rai14 
Ranbp1 
Rapsn  
Rcn3 
Rfx6 
Rfx7 
Ric8b  
Riiad1 
Rnf10 
Rnf38 
Robo4 
Rock1 
Rrp8 
Rsf1 
Runx3 
Ryr1  
Ryr2 
S100a6 
Satb2 
Sbk1 
Scamp5 
Scn4a 
Scnn1b 
Scrib 
Scx 
Serf2  
Shh 
Sirt1 
Slc16a3  
Slc17a6  
Slc22a17 
Slc24a4 
Slc35d1 
Slc39a8 
Slc52a2 
Slc6a2 
Slc6a5 
Slc7a8 
Smarcad1 
Smarcd2 
Smoc1  
Snx10 
Snx27 
Snx3  
Sort1  
Spi1 
Spop 
St14 
Stambp 
Stk38 
Stox2 
Strn3 
Sufu 
Svep1  
Synj1  
Synrg  
Tbr1 
Tbx4 
Tead1 
Tfeb 
Tgfb3 
Thra 
Tmem100 
Tmem115 
Tmem132a  
Tmem161b  
Tmtc3 
Tnni1 
Tnni2 
Tor1aip1  
Tra2a 
Trib1 
Trib2 
Trim59 
Trp73 
Tspan14 
Tulp3 
Tusc3 
Txlna 
Ube2b 
Ube2c 
Ublcp1 
Ubr2 
Ubr4 
Ubxn7 
Uhrf2 
Usf3 
Usp14 
Usp3 
Vps37d  
Vps4a 
Wfdc2  
Wnt1 
Wnt6 
Xbp1 
Xpot 
Ywhaz 
Zfp148 
Zfp260 
Zfp536 
Zscan21 
Zwilch 

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