Mouse Production and Phenotyping
Our phenotyping centres have standardised allele production to knock out genes and pre-defined phenotyping tests to characterise mouse phenotypes. Our phenotyping tests form different ‘phenotyping pipelines’. Our phenotyping pipelines provide an exemplar of the potential of high-throughput pipelines for the acquisition of broad-based phenotype data at both embryonic and adult time points. The range of phenotyping platforms ensures the recovery of phenotype data across multiple systems and disease states. Wild-type mice are continuously run through our phenotyping pipelines, giving us a constantly growing data-set.
Non-viable lines for which pups do not survive birth or long after enter the embryonic pipeline. The embryonic pipeline aims to establish the window of lethality of the embryos and includes various imaging techniques to identify abnormalities in developing embryos.
Viable heterozygote knockouts go through the adult pipeline.
All viable mouse lines go through our comprehensive adult pipeline where tests are performed over several weeks starting at 9 weeks.
A number of lines also go through an ageing process and are again put through our comprehensive phenotyping pipeline from 1 year of age to identify late-onset phenotypes. Many human diseases arise later in life, and this pipeline may help us to find out more about age-related diseases in the human population.
More information about our phenotyping pipelines and procedures is available on IMPReSS