Spleen Immunophenotyping: Images associated with FACS analysis
WT Images
15 weeks old
Mature NK cells - % of NK cellls: 79.014
15 weeks old
Resting Treg cells - % of Treg: 58.223
15 weeks old
Conventional DC (cDC) - % of CD45+: 1.509
15 weeks old
Resting CD4- NKT cells - % of CD4- NKT cells: 65.42
15 weeks old
CD11b-low cDC - % of cDC: 44.004
15 weeks old
KLRG1+ CD4+ T helper cells - % of CD45+: 0.16
15 weeks old
Effector gd cells - % of CD45+: 0.504
15 weeks old
Immature NK cells - % of lymphocytes: 0.621
15 weeks old
Total CD4- NKT cells - % of CD45+: 0.33
15 weeks old
CD4+ T helper cells - % of CD4+: 88.702
15 weeks old
B1a cells - % of B cells: 1.383
15 weeks old
Germinal centre B cells - % of CD45+: 0.24
15 weeks old
Marginal zone B cells - % of B2 cells: 6.071
15 weeks old
CD11b-low cDC - % of CD45+: 0.664
15 weeks old
Macrophages - % of CD45+: 0.616
15 weeks old
Effector Treg cells - % of CD45+: 0.617
15 weeks old
Effector CD8+ T cells - % of CD8+: 6.136
15 weeks old
Ly6C+ NKT cells - % of lymphocytes: 0.53
15 weeks old
Mature NK cells - % of lymphocytes: 2.337
15 weeks old
Naive CD8+ T cells - % of CD45+: 5.623
15 weeks old
Plasma cells - % of CD45+: 0.106
15 weeks old
Effector NK cells - % of CD45+: 0.361
15 weeks old
B2 t1 + t2 cells - % of B2 cells: 9.78
15 weeks old
Resting CD4+ T helper cells - % of CD4+ T helper cells: 70.523
15 weeks old
KLRG1+ Treg cells - % of CD45+: 0.131
15 weeks old
CD5+ gd T cells - % of CD45+: 0.499
15 weeks old
Effector CD4- NKT cells - % of CD4- NKT cells: 34.58
15 weeks old
CD11b-high cDC - % of CD45+: 0.84
15 weeks old
KLRG1+ CD4+ T helper cells - % of CD4+ T helper cells: 1.394
15 weeks old
Total ab T cells - % of CD45+: 22.716
15 weeks old
Effector CD8+ T cells - % of CD45+: 0.591
15 weeks old
Effector CD4+ T helper cells - % of CD45+: 3.361
15 weeks old
Total gd T cells - % of CD45+: 1.223
15 weeks old
Plasma cells - % of B2 cells: 0.169
15 weeks old
Resting CD4+ NKT cells - % of CD4+ NKT cells: 16.46
15 weeks old
Plasmacytoid DC - % of DC: 19.041
15 weeks old
Total CD4+ T cells - % of CD45+: 12.97
15 weeks old
Effector gd T cells - % of gd T cells: 41.226
15 weeks old
Resting CD8+ T cells - % of CD45+: 3.147
15 weeks old
Total CD8+ T cells - % of ab T cells: 42.423
15 weeks old
Effector CD4+ NKT cells - % of CD45+: 0.268
15 weeks old
Marginal zone precursor B cells - % of CD45+: 2.948
15 weeks old
CD103+ CD11b-low cDC - % of CD45+: 0.064
15 weeks old
Resting CD8+ T cells - % of CD8+: 32.653
15 weeks old
Total B2 cells - % of B cells: 98.617
15 weeks old
Resting NK cells - % of CD45+: 2.079
15 weeks old
Total CD4+ T cells - % of ab T cells: 57.099
15 weeks old
Total Treg cells - % of CD45+: 1.486
15 weeks old
Immature NK cells - % of NK cells: 20.986
15 weeks old
Ly6C+ immature NK cells - % of lymphocytes: 0.09
Mutant Images
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4- NKT cells - % of CD45+: 0.045
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD8+ T cells - % of CD45+: 0.208
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4- NKT cells - % of CD45+: 0.151
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total CD4+ NKT cells - % of CD45+: 0.774
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of CD45+: 0.854
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4+ NKT cells - % of CD45+: 0.26
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of NK cells: 59.365
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total NKT cells - % of CD45+: 0.267
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total CD4+ NKT cells - % of CD45+: 0.251
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4+ NKT cells - % of CD45+: 0.048
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of CD45+: 1.864
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total CD4+ NKT cells - % of CD45+: 0.123
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total NKT cells - % of CD45+: 0.442
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Resting CD8+ T cells - % of CD8+: 31.86
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total NKT cells - % of CD45+: 0.581
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4+ NKT cells - % of CD45+: 0.068
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total NKT cells - % of CD45+: 1.593
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4- NKT cells - % of CD45+: 0.037
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of NK cells: 59.576
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4+ NKT cells - % of CD45+: 0.092
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of CD45+: 1.471
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD8+ T cells - % of CD45+: 0.259
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Resting CD8+ T cells - % of CD8+: 31.991
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of NK cells: 61.82
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of CD45+: 2.978
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4- NKT cells - % of CD45+: 0.063
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total NKT cells - % of CD45+: 0.343
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of NK cells: 47.666
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD8+ T cells - % of CD45+: 0.326
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4- NKT cells - % of CD45+: 0.08
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4+ NKT cells - % of CD45+: 0.129
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Resting CD8+ T cells - % of CD8+: 30.415
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total CD4+ NKT cells - % of CD45+: 0.097
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD8+ T cells - % of CD45+: 0.704
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Resting CD8+ T cells - % of CD8+: 38.244
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD8+ T cells - % of CD45+: 0.307
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total NKT cells - % of CD45+: 0.243
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total CD4+ NKT cells - % of CD45+: 0.206
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD8+ T cells - % of CD45+: 0.498
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of CD45+: 0.582
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of NK cells: 41.281
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4+ NKT cells - % of CD45+: 0.205
16 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Total CD4+ NKT cells - % of CD45+: 0.069
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Resting CD8+ T cells - % of CD8+: 29.966
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Resting CD8+ T cells - % of CD8+: 34.046
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of NK cells: 60.634
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
Effector CD4- NKT cells - % of CD45+: 0.049
15 weeks old
Clpptm1a(EUCOMM)Wtsi homozygote
KLRG1+ NK cells - % of CD45+: 2.029
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