Spleen Immunophenotyping: Images associated with FACS analysis
WT Images
15 weeks old
Effector CD4- NKT cells - % of CD4- NKT cells: 34.58
15 weeks old
Effector Treg cells - % of Treg: 41.508
15 weeks old
CD5+ gd T cells - % of gd T cells: 40.763
15 weeks old
Total B2 cells - % of CD45+: 62.752
15 weeks old
Naive CD8+ T cells - % of CD8+: 58.347
15 weeks old
Total CD4- NKT cells - % of CD45+: 0.33
15 weeks old
Ly6C+ mature NK cells - % of lymphocytes: 0.946
15 weeks old
Total B cells - % of CD45+: 63.632
15 weeks old
Late germinal centre B cells - % of GC cells: 3.199
15 weeks old
Germinal centre B cells - % of CD45+: 0.24
15 weeks old
Immature NK cells - % of NK cells: 20.986
15 weeks old
Transitional 1 B cells - % of B2 cells: 5.96
15 weeks old
Germinal centre B cells - % of B2 cells: 0.382
15 weeks old
CD103+ CD11b-low cDC - % of CD11b-low cDC: 9.615
15 weeks old
Effector gd T cells - % of gd T cells: 41.226
15 weeks old
Ly6C+ NKT cells - % of lymphocytes: 0.53
15 weeks old
Mature NK cells - % of lymphocytes: 2.337
15 weeks old
Effector NK cells - % of CD45+: 0.361
15 weeks old
B2 t1 + t2 cells - % of B2 cells: 9.78
15 weeks old
CD4+ T helper cells - % of CD4+: 88.702
15 weeks old
KLRG1+ NK cells - % of NK cells: 31.829
15 weeks old
KLRG1+ Treg cells - % of CD45+: 0.131
15 weeks old
B1a cells - % of CD45+: 0.88
15 weeks old
CD5+ gd T cells - % of CD45+: 0.499
15 weeks old
KLRG1+ NK cells - % of CD45+: 0.776
15 weeks old
Granulocytes - % of CD45+: 1.376
15 weeks old
CD11b-high cDC - % of CD45+: 0.84
15 weeks old
Plasma cells - % of B2 cells: 0.169
15 weeks old
Mature NK cells - % of NK cellls: 79.014
15 weeks old
Total CD4+ NKT cells - % of NKT cells: 49.412
15 weeks old
Transitional 1 B cells - % of CD45+: 3.74
15 weeks old
Plasmacytoid DC - % of CD45+: 0.355
15 weeks old
Effector CD8+ T cells - % of CD45+: 0.591
15 weeks old
Total CD4+ NKT cells - % of CD45+: 0.322
15 weeks old
Marginal zone B cells - % of CD45+: 3.81
15 weeks old
Conventional DC (cDC) - % of DC: 80.959
15 weeks old
Effector CD4+ T helper cells - % of CD45+: 3.361
15 weeks old
Total gd T cells - % of CD45+: 1.223
15 weeks old
Total B2 cells - % of B cells: 98.617
15 weeks old
Effector CD8+ T cells - % of CD8+: 6.136
15 weeks old
Total CD4+ T cells - % of CD45+: 12.97
15 weeks old
Effector CD4+ NKT cells - % of CD45+: 0.268
15 weeks old
Marginal zone precursor B cells - % of CD45+: 2.948
15 weeks old
Total NK cells - % of CD45+: 2.437
15 weeks old
Resting gd T cells - % of gd T cells: 57.82
15 weeks old
Resting CD4+ NKT cells - % of CD4+ NKT cells: 16.46
15 weeks old
B1a cells - % of B cells: 1.383
15 weeks old
Resting gd T cells - % of CD45+: 0.707
15 weeks old
Ly6C+ immature NK cells - % of lymphocytes: 0.09
15 weeks old
Resting CD4+ T helper cells - % of CD4+ T helper cells: 70.523
Mutant Images
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD8+ T cells - % of ab T cells: 32.705
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD4+ T cells - % of ab T cells: 63.768
15 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD8+ T cells - % of ab T cells: 39.949
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD4+ T cells - % of ab T cells: 66.796
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD8+ T cells - % of ab T cells: 35.748
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD8+ T cells - % of ab T cells: 37.374
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD8+ T cells - % of ab T cells: 36.07
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD4+ T cells - % of ab T cells: 63.494
16 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD4+ T cells - % of ab T cells: 62.116
15 weeks old
Brd2em1(IMPC)Wtsi heterozygote
Total CD4+ T cells - % of ab T cells: 59.575
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