Spleen Immunophenotyping: Images associated with FACS analysis
WT Images
15 weeks old
Mature NK cells - % of NK cellls: 79.014
15 weeks old
Resting Treg cells - % of Treg: 58.223
15 weeks old
Conventional DC (cDC) - % of CD45+: 1.509
15 weeks old
Resting CD4- NKT cells - % of CD4- NKT cells: 65.42
15 weeks old
CD11b-low cDC - % of cDC: 44.004
15 weeks old
KLRG1+ CD4+ T helper cells - % of CD45+: 0.16
15 weeks old
Effector gd cells - % of CD45+: 0.504
15 weeks old
Immature NK cells - % of lymphocytes: 0.621
15 weeks old
Total CD4- NKT cells - % of CD45+: 0.33
15 weeks old
CD4+ T helper cells - % of CD4+: 88.702
15 weeks old
B1a cells - % of B cells: 1.383
15 weeks old
Germinal centre B cells - % of CD45+: 0.24
15 weeks old
Marginal zone B cells - % of B2 cells: 6.071
15 weeks old
CD11b-low cDC - % of CD45+: 0.664
15 weeks old
Macrophages - % of CD45+: 0.616
15 weeks old
Effector Treg cells - % of CD45+: 0.617
15 weeks old
Effector CD8+ T cells - % of CD8+: 6.136
15 weeks old
Ly6C+ NKT cells - % of lymphocytes: 0.53
15 weeks old
Mature NK cells - % of lymphocytes: 2.337
15 weeks old
Naive CD8+ T cells - % of CD45+: 5.623
15 weeks old
Plasma cells - % of CD45+: 0.106
15 weeks old
Effector NK cells - % of CD45+: 0.361
15 weeks old
B2 t1 + t2 cells - % of B2 cells: 9.78
15 weeks old
Resting CD4+ T helper cells - % of CD4+ T helper cells: 70.523
15 weeks old
KLRG1+ Treg cells - % of CD45+: 0.131
15 weeks old
CD5+ gd T cells - % of CD45+: 0.499
15 weeks old
Effector CD4- NKT cells - % of CD4- NKT cells: 34.58
15 weeks old
CD11b-high cDC - % of CD45+: 0.84
15 weeks old
KLRG1+ CD4+ T helper cells - % of CD4+ T helper cells: 1.394
15 weeks old
Total ab T cells - % of CD45+: 22.716
15 weeks old
Effector CD8+ T cells - % of CD45+: 0.591
15 weeks old
Effector CD4+ T helper cells - % of CD45+: 3.361
15 weeks old
Total gd T cells - % of CD45+: 1.223
15 weeks old
Plasma cells - % of B2 cells: 0.169
15 weeks old
Resting CD4+ NKT cells - % of CD4+ NKT cells: 16.46
15 weeks old
Plasmacytoid DC - % of DC: 19.041
15 weeks old
Total CD4+ T cells - % of CD45+: 12.97
15 weeks old
Effector gd T cells - % of gd T cells: 41.226
15 weeks old
Resting CD8+ T cells - % of CD45+: 3.147
15 weeks old
Total CD8+ T cells - % of ab T cells: 42.423
15 weeks old
Effector CD4+ NKT cells - % of CD45+: 0.268
15 weeks old
Marginal zone precursor B cells - % of CD45+: 2.948
15 weeks old
CD103+ CD11b-low cDC - % of CD45+: 0.064
15 weeks old
Resting CD8+ T cells - % of CD8+: 32.653
15 weeks old
Total B2 cells - % of B cells: 98.617
15 weeks old
Resting NK cells - % of CD45+: 2.079
15 weeks old
Total CD4+ T cells - % of ab T cells: 57.099
15 weeks old
Total Treg cells - % of CD45+: 1.486
15 weeks old
Immature NK cells - % of NK cells: 20.986
15 weeks old
Ly6C+ immature NK cells - % of lymphocytes: 0.09
Mutant Images
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Immature NK cells - % of lymphocytes: 0.41
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Resting NK cells - % of CD45+: 0.811
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Macrophages - % of CD45+: 0.495
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Macrophages - % of CD45+: 0.132
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Mature NK cells - % of lymphocytes: 2.281
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Immature NK cells - % of lymphocytes: 0.441
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Total NK cells - % of CD45+: 1.593
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Total NK cells - % of CD45+: 1.07
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Resting NK cells - % of CD45+: 1.782
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Resting NK cells - % of CD45+: 1.182
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Mature NK cells - % of lymphocytes: 0.747
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Mature NK cells - % of lymphocytes: 0.793
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Total NK cells - % of CD45+: 1.953
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Macrophages - % of CD45+: 0.374
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Immature NK cells - % of lymphocytes: 0.592
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Mature NK cells - % of lymphocytes: 0.976
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Macrophages - % of CD45+: 0.252
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Resting NK cells - % of CD45+: 1.272
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Resting NK cells - % of CD45+: 1.06
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Mature NK cells - % of lymphocytes: 0.971
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Macrophages - % of CD45+: 1.006
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Immature NK cells - % of lymphocytes: 0.46
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Macrophages - % of CD45+: 0.302
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Mature NK cells - % of lymphocytes: 1.426
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Total NK cells - % of CD45+: 2.314
15 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Total NK cells - % of CD45+: 1.451
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Immature NK cells - % of lymphocytes: 0.969
16 weeks old
Spink14tm1e(KOMP)Wtsi homozygote
Immature NK cells - % of lymphocytes: 0.581
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