Search Results - IMPReSS

FACS

Pipeline Key: ICS_001

Procedure Key: ICS_IMM_001

Immunophenotyping of immune system cells

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ALTERNATIVE - FACS

Pipeline Key: ALTICS_001

Procedure Key: ALTICS_IMM_001

ALTERNATIVE - Immunophenotyping of immune system cells

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FACS

Pipeline Key: HRWL_001

Procedure Key: HRWL_IMM_001

Immunophenotyping (FACS) assay performed on splenocytes. This test corresponds to the protocol being performed at Harwell before the Toronto 2013 Immunophenotyping protocol agreement.

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ALTERNATIVE - FACS

Pipeline Key: ALTHRWL_001

Procedure Key: ALTHRWL_IMM_001

ALTERNATIVE - Immunophenotyping (FACS) assay performed on splenocytes. This test corresponds to the protocol being performed at Harwell before the Toronto 2013 Immunophenotyping protocol agreement.

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FACs Analysis

Pipeline Key: HAS_001

Procedure Key: HAS_FAC_003

The characterisation of of main leukocyte subsets in peripheral blood cells from mice by flow cytometry.Reliable values of frequencies of leukocyte clusters are very much dependent on the appropriate preparation, acquisition and gating of leukocytes. This methodinvolves ammonium chloride erythrocyte lyses, thus preventing interference by larger amounts of erythrocytes. For all stainings, a monoclonal antibody to thecell surface glycoprotein CD45 is used to be able to create a CD45+ gate, allowing discrimination of leukocytes from debris, erythrocytes and thrombocytes. Furthermore, staining with propidium iodide (PI) gates out dead cells. Samples are acquired until a number of 30000 living CD45+ cells is reached for each sample (ref Adler and Busch 2007, submitted). Data are compensated analysed and using FlowJo software.This protocol is part of the EMPReSSslim pipeline and is carried out on a cohort of 10 males and 10 females (age matched) at a time.

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FACs Analysis

Pipeline Key: ESLIM_002

Procedure Key: ESLIM_018_001

The characterisation of of main leukocyte subsets in peripheral blood cells from mice by flow cytometry.Reliable values of frequencies of leukocyte clusters are very much dependent on the appropriate preparation, acquisition and gating of leukocytes. This methodinvolves ammonium chloride erythrocyte lyses, thus preventing interference by larger amounts of erythrocytes. For all stainings, a monoclonal antibody to thecell surface glycoprotein CD45 is used to be able to create a CD45+ gate, allowing discrimination of leukocytes from debris, erythrocytes and thrombocytes. Furthermore, staining with propidium iodide (PI) gates out dead cells. Samples are acquired until a number of 30000 living CD45+ cells is reached for each sample (ref Adler and Busch 2007, submitted). Data are compensated analysed and using FlowJo software.This protocol is part of the EMPReSSslim pipeline and is carried out on a cohort of 10 males and 10 females (age matched) at a time.

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FACs Analysis

Pipeline Key: M-G-P_001

Procedure Key: M-G-P_023_001

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ALTERNATIVE - FACs Analysis

Pipeline Key: ALTHAS_001

Procedure Key: ALTHAS_FAC_003

ALTERNATIVE - The characterisation of of main leukocyte subsets in peripheral blood cells from mice by flow cytometry.Reliable values of frequencies of leukocyte clusters are very much dependent on the appropriate preparation, acquisition and gating of leukocytes. This methodinvolves ammonium chloride erythrocyte lyses, thus preventing interference by larger amounts of erythrocytes. For all stainings, a monoclonal antibody to thecell surface glycoprotein CD45 is used to be able to create a CD45+ gate, allowing discrimination of leukocytes from debris, erythrocytes and thrombocytes. Furthermore, staining with propidium iodide (PI) gates out dead cells. Samples are acquired until a number of 30000 living CD45+ cells is reached for each sample (ref Adler and Busch 2007, submitted). Data are compensated analysed and using FlowJo software.This protocol is part of the EMPReSSslim pipeline and is carried out on a cohort of 10 males and 10 females (age matched) at a time.

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ALTERNATIVE - FACs Analysis

Pipeline Key: ALTESLIM_002

Procedure Key: ALTESLIM_018_001

ALTERNATIVE - The characterisation of of main leukocyte subsets in peripheral blood cells from mice by flow cytometry.Reliable values of frequencies of leukocyte clusters are very much dependent on the appropriate preparation, acquisition and gating of leukocytes. This methodinvolves ammonium chloride erythrocyte lyses, thus preventing interference by larger amounts of erythrocytes. For all stainings, a monoclonal antibody to thecell surface glycoprotein CD45 is used to be able to create a CD45+ gate, allowing discrimination of leukocytes from debris, erythrocytes and thrombocytes. Furthermore, staining with propidium iodide (PI) gates out dead cells. Samples are acquired until a number of 30000 living CD45+ cells is reached for each sample (ref Adler and Busch 2007, submitted). Data are compensated analysed and using FlowJo software.This protocol is part of the EMPReSSslim pipeline and is carried out on a cohort of 10 males and 10 females (age matched) at a time.

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