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Purpose
Full-field electroretinogram (ERG) is a mass electrical response of the retina to a light stimulus. The ERG contains four components: a-wave, b-wave, c-wave and FO-wave. These four components reflect the responsiveness of retinal photoreceptor cells and other neurons as a measure of visual function.
Experimental Design
Equipment
Celeris - Diagnosys LLC
Procedure
Procedure
- Transfer mice from the animal room to the testing room.
- Mice are dark adapted overnight (alternatively: minimum of 2 hours) for testing procedures.
- All rod testing procedures are done under dim red light after a minimum 2-hour dark adaptation period
Notes
Amplitude and timing measures of the ERG waveform are taken:
- The a-wave amplitude is measured from the pre-stimulus baseline to the lowest negative trough; the b-wave amplitude is measured from the trough of the a-wave to the following highest peak; the c-wave amplitude is measured from the pre-stimulus baseline to the following highest peak; the FO- wave amplitude is measured from the peak of the c-wave to the subsequent lowest trough.
- Implicit time (t) is measured from stimulus onset to the trough or peak of each wave.