Jump to:
Purpose
To assess visible morphological defects in E9.5 placentas from lethal strains
Experimental Design
Procedure
- Set up timed mating with heterozygous animals. Dissect at a consistent time and collect >=2 placentas from homozygote embryos. Coordination with viability screen is at the centres discretion.
- Assess placentas according to Gross Morphology parameters.
- Generate gross images of placentas (optional) with scored defects and control placentas.
- Collect tissue for genotyping
- Process placentas for Histopathology, or other imaging (OPTIONAL - depending on center pipeline)
- Scores will be shown per placenta and split by zygosity.
Notes
All genotypes should be collected using validated assays.
Y chromosome assay required for X-linked lethal strains.
Placentas may be processed for Histopathology or 3D Imaging