Gross Morphology Placenta E9.5 IMPC_GPL_001

Purpose

To assess visible morphological defects in E9.5 placentas from lethal strains

Experimental Design

  • Set up timed matings with heterozygous mice
  • Day 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.
  • Collect placentas at E9.5 (at least 2 homozygotes scored)
  • Capture gross images (optional)
  • Collect tissue and genotype embryos.

Procedure

  1. Set up timed mating with heterozygous animals. Dissect at a consistent time and collect >=2 placentas from homozygote embryos. Coordination with viability screen is at the centres discretion.
  2. Assess placentas according to Gross Morphology parameters.
  3. Generate gross images of placentas (optional) with scored defects and control placentas.
  4. Collect tissue for genotyping
  5. Process placentas for Histopathology, or other imaging (OPTIONAL - depending on center pipeline)
  6. Scores will be shown per placenta and split by zygosity.

Notes

All genotypes should be collected using validated assays.

Y chromosome assay required for X-linked lethal strains.

Placentas may be processed for Histopathology or 3D Imaging

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