Fertility of Homozygous Knock-out Mice IMPC_FER_001

Purpose

To assess the fertility of homozygous knockout mice.

Experimental Design

  • Minimum 1 hom x hom mating for fertile mice and minimum of 2 failed hom x hom matings attempts to proceed to secondary screen.
    • Secondary screen:  Minimum 2 Hom x 1 WT or Het (Male) , Minimum 2 Hom x 1 WT or Het (Female)
  • Minimal age at test = minimal age at start of procedure = 8 weeks.
  • Maximum age at test = To be confirmed, proposed 14 weeks
  • Minimum length of test (time mice left in mating) 4 - 6 weeks

Procedure

  1. Homozygous mice (minimum age of 8 weeks, maximum age of 14 weeks) are mated for 4-6 weeks. Strains that produce no progeny or pregnant dams after 4 to 6 weeks progress to secondary screening.
  2. Secondary Male Infertility Screen:
    1. Set up 2 separate matings: male Hom x female WT or Het
      1. Observe matings for 4 to 6 weeks;
      2. Matings that result in visibly pregnant females (confirmed by dissection) or pups will be scored MALE FERTILE.
      3. Matings that do not result in pregnancy will be scored MALE INFERTILITY.
  3. Secondary Female Infertility Screen
    1. Set up  2 separate matings: female Hom x male WT or Het
    2. Observe matings for 4 to 6 weeks;
      1. Matings that result in visibly pregnant females (confirmed by dissection) or pups will be scored FEMALE FERTILE.
      2. Matings that do not result in pregnancy will be scored FEMALE INFERTILITY.

Notes

Pregnancies should be confirmed.

All annotations are based on yes/no scores.

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