Tissue Embedding and Block Banking IMPC_BLK_001

Purpose

Collect and fix a standard list of tissues from the complete necropsy (see IMPC Gross Pathology & Tissue Collection SOP)

Trim the fixed tissues into cassettes for processing

Embed the tissues in paraffin in a standard orientation

Experimental Design

Minimum number of mutant animals: 2 mutant males + 2 mutant females

Minimum age of animals: 17 weeks

Procedure

  1. The following 29 tissues are collected and wet fixed at necropsy (according to IMPC Gross Pathology & Tissue Collection SOP)

 

Adrenal gland

Heart

Mammary gland

Skin

Thymus

Brain

Kidney

Ovary

Small intestine

Thyroid

Epididymis

Large intestine

Pancreas

Spinal cord

Trachea

Esophagus

Liver

Prostate

Spleen

Urinary bladder

Eye with optic nerve

Lung

Seminal vesicles

Stomach

Uterus

Gall bladder

Lymph node

Skeletal muscle

Testes

 

 

 

  1. Trim the fixed tissue into labelled cassettes for processing according to Centre-specific SOP
  2. Process the cassettes according to Centre-specific SOP
  3. Embed the tissues in paraffin using the orientation below:

 

Tissue

Number of Tissue Pieces

Embedding Orientation

Brain

Up to 5

Transverse or Mid-sagittal

Eye with optic nerve

1 (per eye)

Sagittal (optic nerve parallel to cassette surface)

 

1 (per eye)

Sagittal (within skull)

Spinal cord

Up to 6

Cross

Thymus

1

Flat

Thyroid

1 (with trachea)

Cross

Heart

1 or 2

Cross or Sagittal

Trachea

1 (with thyroid)

Cross

Esophagus

1

Cross

Lung

Up to 5

Cross or Sagittal

Liver

Up to 2

Cross

Gall bladder

1 (with liver)

Cross

Stomach

Up to 2

Cross

Small intestine

1

Cross

Large intestine

1

Cross

Pancreas

1

Flat

Spleen

1

Flat or Cross

Kidney

1 or 2

Cross and Sagittal

Adrenal gland

At least 1

Cross or Sagittal

Mammary gland

At least 1

Flat

Lymph node

At least 1

Flat

Skin

Up to 5

Cross on edge

Skeletal muscle

At least 1

Cross

Urinary bladder

1

Flat

Testes

At least 1

Flat

Epididymis

At least 1

Flat

Prostate

1

Flat

Seminal vesicles

At least 1

Flat

Ovary

At least 1

Flat

Uterus

1

Flat or Cross

 

Notes

The age of the animals at which they undergo this procedure is an important data. It is, however, derived from the date of birth and the date of necropsy of the animal, the latter being a metadata parameter. Similar situation applies to the number of animals that a centre has submitted to this procedure – it will also be derived and subject to quality control of the data.

Parameters & Metadata

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