Electroretinography JAX_ERG_001

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Purpose

Full-field electroretinogram (ERG) is a mass electrical response of the retina to a light stimulus.  The ERG contains two components, reflecting the response of the photoreceptors (a-wave) and ON bipolar cells (b-wave; Kofujj et al., 2000 J Neurosci 20: 5733-5740, 2000)

Experimental Design

 

Procedure

  1. Transfer mice from animal room 4435 to the testing room 4435C
  2. Mice are weighed, and 1 drop of Atropine is placed in right eye (unless eye is missing, small, or otherwise effected) to induce mydriasis.
  3. Place mice in dark adaption chamber.
    1. All rod testing procedures are done under dim red light after 2 hour dark adaptation
  4. Ketaset/Xylazine /saline solution (0.1ml/20g body weight) is injected (i.p.) to induce anesthesia and 1 drop of Cyclomydril (ophthalmic solutions) are applied to induce mydriasis.
  5. Once the pupil has dilated and anesthetic agents have taken affect, the mouse is placed on a heating pad (38°C) and the electrodes are attached
    1. Tail (ground)
    2. Left cheek (reference)
    3. Cornea (recording)
      1. Use one drop of Goniosol to moisten cornea
  6. The rod test is performed with a short-wavelength Wratten filter (# 47A), using stimulation intensities of 8 lumen sec/ft2, and 0.2 HZ frequency, every 10 seconds.
  7. Data is saved and graphed
  8. After the dark-adapted tests are run, inject next mouse and put back into dark box.  Turn on a steady light to isolate cone responses in rod tested mouse.
  9. After 8-10 minutes of light adaptation, cone ERGs are obtained to white strobe flashes presented at 1 Hz, with intensities of 16 lumen sec/ft2, every 1 second for 20 seconds.
  10. Carefully remove the recording electrodes.  Anaesthesia reversal is promoted with a subQ injection of (~0.5ml) sterile saline and mouse recovers on a heating pad until fully conscious.
  11. Data is saved and graphed
  12. Open indexing program and index and save rods and cones tests.

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