Hargreaves Test JAX_HRG_001

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Purpose

To assess thermal hyperalgesia in the mouse paw.

Experimental Design

Minimum number of mutant animals: 7 males + 7 females

Age at test: either 8 weeks or 17 weeks

Sexual dimorphism:

Procedure

  1. Baseline measurement
    1. Transfer mouse to the testing arena and allow to acclimate to the test environment.
    2. Ensure that the intensity and cut-off timer of the radiant heat source are set correctly.
    3. Position the light source below the platform and directed at the correct part of the paw.
    4. Turn the light source on and record the latency to paw withdrawal. The stimulus is terminated once a response is registered, or if the maximum exposure time is reached before the animal responds.
    5. Repeat the test so that each mouse is tested three times with the same paw.
  2. Challenge
    1. The challenge is administered after the baseline measurement has been completed.
    2. Anaesthetise the mouse and administer the challenge injection.
  3. Test 1
    1. After the specified length of time since the challenge injection, the mouse is re-tested using the same procedure as described for the baseline measurement.
  4. Test 2
    1. After the specified length of time since the challenge injection, the mouse is re-tested again using the same procedure as described for the baseline measurement.

Notes

This procedure is a pilot study from the Pain Phenotyping Pilot

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