MicroCT E9.5 IMPC_EML_001

Purpose

To assess morphological defects in E9.5 embryos from lethal strains with MicroCT.

Experimental Design

  • Set up timed matings with heterozygous mice
  • Day 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.
  • Collect embryos at E9.5 with 3 minimum homozygous and 1 littermate control
  • Ensure embryos are bleed as much as possible.
  • Reconstruct the scans according to manufacturer's instructions.

Procedure

  1. Set up timed mating with heterozygous animals. Dissect and collect >=3 homozygote embryos.
  2. Process the embryos using the centre’s staining and embedding protocol . Centre-specific methods  are recorded within a document
  3. Scan and generate reconstructions of embryos.

Notes

Embryos may be processed for Histopathology.

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