Von Frey Test TCP_VFR_001

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Purpose

To detect mechanical/tactile sensitivity in the mouse paw

Experimental Design

Minimum number of mutant animals: 7 males + 7 females

Age at test: 16 weeks

Sexual dimorphism:

Procedure

  1. Baseline measurement
    1. Move the mice to the testing chambers and leave to acclimate for the specified length of time.
    2. Mice should be tested using the simplified up-down method (SUDO) of Bonin et al. (2014).
    3. When the mouse is still, apply the filament to the centre of the right hindpaw. Press the filament against the paw for 3 seconds.
    4. Mark the response '0' if the mouse does not react, or 'X' if it does react to the filament.
    5. Leave the mouse for a minimum of 2 minutes before presentation of the next filament.
    6. The next filament to be tested will depend on the response to the previous filament.
      • If the mice did respond to the previous filament, they should be tested with the filament of the next smallest size. Once tested, record the response.
      • If the mice did not respond to the previous filament, they should be tested with the filament of the next largest size. Once tested, record the response.
    7. Continue testing the mice until 5 trials have been completed.
    8. The procedure is then repeated for a second run.
  2. Challenge
    1. The challenge is administered after the baseline measurement has been completed.
  3. Test 1
    1. 22 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.
  4. Test 2
    1. 142 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.

Notes

This procedure is a pilot study from the Pain Phenotyping Pilot

A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments. Bonin RP, Bories C, De Koninck Y. Mol Pain. 2014 Apr 16;10:26. doi: 10.1186/1744-8069-10-26.PMID:24739328

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