Von Frey Test JAX_VFR_001

Purpose

To investigate mechanical sensitivity in wild type and genetically altered mice.

Experimental Design

Minimum number of mutant animals: 7 males + 7 females

Age at test: 17 weeks

Sexual dimorphism:

Procedure

  1. Baseline measurement
    1. Set up the testing room and equipment. Check the condition of the von Frey filaments.
    2. Place mice into the testing arenas and leave them to habituate for the specified length of time.
    3. Once habituated, test the mouse with the starting filament. When the mouse has all four feet on the platform, position the starting filament below the mid-plantar surface of the foot.
    4. Press the filament against the foot until the filament bends. Hold the filament in position for 3 seconds, or until the mouse moves its foot.
    5. Mark the response as '0' if the mouse does not react, or 'X' if it does react to the filament.
    6. Move on to testing the next mouse with the starting filament.
    7. Once all mice have been tested with the starting filament, test them with the next filament.
      • If the mice did respond to the previous filament, they should be tested with the filament of the next smallest size. Once tested, record the response.
      • If the mice did not respond to the previous filament, they should be tested with the filament of the next largest size. Once tested, record the response.
    8. Continue testing the mice using the up-down pattern until the stated number of trials have been completed.
  2. Challenge
    1. The challenge should be administered 24 hours after the baseline measurement took place.
    2. Prepare the challenge injection.
    3. Anaesthetise the mouse.
    4. Administer the challenge to the plantar surface of the right hind paw.
    5. Monitor the condition of the mouse as it recovers from the anaesthetic.
  3. Test 1
    1. 24 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.
  4. Test 2
    1. 48 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.

Notes

This procedure is a pilot study from the Pain Phenotyping Pilot

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