Von Frey Test HRWL_VFR_001

Purpose

To assess mechanical sensitivity using calibrated von Frey filaments.

Experimental Design

Minimum number of mutant animals: 7 males + 7 females

Age at test: 10 weeks

Sexual dimorphism:

Procedure

  1. Habituation
    1. Place mice in the testing chambers on the elevated wire grid.
    2. Leave each mouse for an hour to acclimatise.
    3. The left hind paw is tested first using the following method.
      1. The 0.6g von Frey filament should be used as the starting filament.
      2. Apply the filament to the plantar surface of the hind paw. The filament should be applied with enough force to cause the filament to bend, and remain in contact for a total of 1-2 seconds.
      3. The size of the next filament to be applied will depend on the response to the previous filament.
        • If no withdrawal response was observed then the next highest filament is tested.
        • If there was a withdrawal response then the next lowest filament is tested.
      4. Continue applying filaments until the paw has been tested 5 times, with at least 2 minutes between each stimulus presentation.
    4. Repeat the testing process with the right hind paw.
  2. Test 1
    1. 24 hours after habituation, re-test the mouse with the von Frey filaments using the same procedure as described for the habituation period.
  3. Test 2
    1. 48 hours after habituation, re-test the mouse with the von Frey filaments using the same procedure as described for the habituation period.

Notes

This procedure is a pilot study from the Pain Phenotyping Pilot

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