Challenge Whole Body Plethysmography ICS_CHL_003

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Purpose

The purpose of this procedure is to record the respiratory function of mice, with a LPS challenge, by means of Unrestrained Whole-Body Plethysmography, using a lipopolysaccharides (LPS) challenge.

Description:  Spontaneous bronchospasm after LPS challenge

 

Experimental Design

 

Equipment

  1. Flow chambers
  2. Plethysmographs
  3. Computer connected to flow chamber
  4. Challenge reagents
  5. Personal safety equipment (masks, gloves, etc.)

Procedure

  1. Before starting, calibrate the plethysmographic chambers
  2. Freshly prepare a 200 µg/mL LPS (O55:B5 serotype) solution in sterile saline (NaCl 0.9 %).
  3. Inject 50 mg/kg ketamine and 3.3 mg/kg xylazine to mice in 100 µL volume by intraperitoneal injection
  4. Administrate 25 µL of LPS solution (5 µg / mice)
  5. Mouse are next placed in decubitus on a heated plate during 10-15 min 
  6. Place mice in the chambers
  7. Start the experimental recording phase (6 hours)
  8. Remove mice from chambers and place back in the home cages.

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