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Purpose
To detect abnormalities in eye morphology.
Experimental Design
Procedure
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Turn on the OCT and start the database
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Anaesthetize mouse
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Prepare mouse eyes with drops and place contact lens (focal length 10 mm) on the right eye
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Enter mouse data in the “Create new patient file” area and switch to the “Acquisition” window
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Move the OCT camera to the right position and activate measurement modus
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Place mouse collaterally to the OCT camera on the right side of a platform that is fixed in front of the OCT lens
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Search the contact lens in the live picture of the fundus image field and place the pupil of the mouse eye in the centre of the window
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Move the OCT camera such that OCT lens and contact lens come close to each other
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Focus the fundus picture by slightly moving up/down or forward/backward, and lock the camera at this position
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Take en face fundus images
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Set the "Ref.Arm“ ruler such that the section of the retina is placed in the centre of the blue rectangle
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Set the mode of measurement on SD-OCT modus
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Move the green horizontal line in the fundus image field to the optic nerve level
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Save images of retinal sections
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Move the OCT camera to the left position
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Repeat measurement procedure for the left eye
Notes
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As a minimum, all abnormalities should be imaged.
- Where capacity permits, all mice can be imaged
- Majority of parameters can be analysed using the standard approach for assessing categorical data. To increase power for analysis purposes, where an abnormality is detected in the left, right or both eyes, the data may be combined to generate one "abnormal" category.
Data QC
Image QC is typically performed during data collection to ensure high quality images are captured whilst eyes are dilated etc.