Phenotyping Protocols for Pipeline: Harwell late adult HRWLLA_001

Unrestricted
Tick Mandatory
Body Weight HRWLLA_BWT_001 The body weight test measures the weight of the mouse in a time series, allowing monitoring of its evolution; also, it is required in many other procedures.
Week 52
Tick Mandatory
Open Field HRWLLA_OFD_001 The Open Field test is used to assess anxiety and exploratory behaviors. It is based on the natural tendency of an animal to explore and to protect itself using avoidance which translates to a normal animal spending more time in the periphery of the Open Field arena than in the center (the most anxiogenic area).
Tick Mandatory
Combined SHIRPA and Dysmorphology HRWLLA_CSD_003 SHIRPA and dysmorphology were originally always separate assessments.  However they have recently been combined as assessments, so that they take place at the same time.The purpose of the assessments is to examine mice for obvious physical characteristics, behaviors and morphological abnormalities.Descriptions include abnormal locomotion/appearance/behavior/reflex reactions.
Tick Mandatory
Grip Strength HRWLLA_GRS_001 The grip strength test is used to measure the neuromuscular function as maximal muscle strength of forelimbs and combined forelimbs and hind limbs. These are assessed by the grasping applied by the mouse on a grid that is connected to a sensor. Three trials are carried out in succession measuring forelimb-strength only, followed by three successive trials measuring the combined forelimb/hindlimb grip strength. All grip strength values obtained are normalized against mouse body weight.Ontological description: MP:0001515 - abnormal grip strength.
Week 54
Tick Mandatory
Week 55
Electrocardiogram (ECG) HRWLLA_ECG_001 To provide a high throughput method to obtain Electrocardiograms in a conscious mouse.
Echo HRWLLA_ECH_001 To assess the functionality of the heart in order to determine the presence of a mutant phenotype.
Electrocardiogram (ECG) HRWLLA_ECG_002 To provide a high throughput method to obtain Electrocardiograms in a conscious mouse.
Week 56
Tick Mandatory
Intraperitoneal glucose tolerance test (IPGTT) HRWLLA_IPG_001 The glucose tolerance test measures the clearance of an intraperitoneally injected glucose load from the body. It is used to detect disturbances in glucose metabolism that can be linked to human conditions such as diabetes or metabolic syndrome. Animals are fasted for approximately 16 hours, fasted blood glucose levels are determined before a solution of glucose is administered by intra-peritoneal (IP) injection. Subsequently, the blood glucose level is measured at different time points during the following 2 hours.Ontological description: MP:0005559 - increased circulating glucose level, MP:0005560 - decreased circulating glucose level, MP:0005293 - impaired glucose tolerance, MP:0005292 - improved glucose tolerance, MP:0005291           abnormal glucose tolerance, MP:0000188 - abnormal circulating glucose level.
Week 57
Tick Mandatory
X-ray HRWLLA_XRY_001 Construct and analyse digital X-ray images in immobilised mice using a Faxitron X-Ray system or NTB digital X-ray scanner.
Tick Mandatory
Body Composition (DEXA lean/fat) HRWLLA_DXA_001 Measure bone mineral content and density as well as body composition in mice using the DEXA (Dual Energy X-ray Absorptiometry) analyser.
Week 58
Tick Mandatory
Tick Mandatory
Week 59
Tick Mandatory
Hematology HRWLLA_HEM_002 Hematological assessment of blood determines blood cell counts (white blood cells, red blood cells, hemoglobin, and platelets) and additional hematological parameters (hematocrit, mean cell volume, mean corpuscular hemoglobin, mean cell hemoglobin concentration) can be derived using these indices. These tests will indicate abnormalities in the production of blood and its components (blood cells and hemoglobin) as well as in the associated blood-forming organs. Ontological description: MP:0002429 - abnormal blood cell morphology/development.
Tick Mandatory
Clinical Chemistry HRWLLA_CBC_002 Clinical chemistry determines biochemical parameters in plasma including enzymatic activity, specific substrates and electrolytes. Ontological description: MP:0001545 – blood physiology abnormalities.
Tick Mandatory
Gross Pathology and Tissue Collection HRWLLA_PAT_002 To perform a complete necropsy to detect and record abnormal external findings and macroscopic alterations in internal and external organs, record body and heart weights (see IMPC Heart Weight SOP), and collect a standardized list of tissues for fixation with or without further processing (see non-mandatory IMPC Tissue Embedding & Block Banking SOP).
Tick Mandatory
Tissue Embedding and Block Banking HRWLLA_BLK_001 Collect and fix a standard list of tissues from the complete necropsy (see IMPC Gross Pathology & Tissue Collection SOP)Trim the fixed tissues into cassettes for processingEmbed the tissues in paraffin in a standard orientation
Tick Mandatory
Clinical Chemistry HRWLLA_CBC_003 Clinical chemistry determines biochemical parameters in plasma including enzymatic activity, specific substrates and electrolytes. Ontological description: MP:0001545 – blood physiology abnormalities.