Immunophenotyping IMPC_IMM_001

Purpose

Experimental Design

Equipment

Procedure

Parameters

Metadata

Version Type Req. Upload Req. Analysis Annotation Increment Option Ontology Options Derived Unit Data Type
Equipment name IMPC_IMM_077_001 1.0 procedureMetadata Tick                         FACSFortessa_1LSR IIFlow cytometer         TEXT
Equipment manufacturer IMPC_IMM_078_001 1.0 procedureMetadata Tick Tick                 BD BiosciencesBeckman Coulter         TEXT
Equipment model IMPC_IMM_079_001 1.0 procedureMetadata Tick Tick                 BD LSRFortessa Cell AnalyzerH47100123GalliosFACSAria IIIBD LSR-IICANTO-II         TEXT
CS&T Bead lot IMPC_IMM_080_001 1.0 procedureMetadata Tick                                         TEXT
Anesthesia IMPC_IMM_081_001 1.0 procedureMetadata Tick Tick                 Injection narcosis with Sodium Pentobarbital (Somnopentyl)noneInjection narcosis with Ketamine (100mg/kg)/Xylazine (10mg/kg)Injection narcosis with Tribromoethanol (Avertin)Isoflurane         TEXT
Cell digestion IMPC_IMM_082_001 1.0 procedureMetadata Tick Tick                 GentleMACSmanual         TEXT
Cell digestion temperature (in Celsius) IMPC_IMM_106_001 1.1 procedureMetadata Tick                         37RT         TEXT
Cell digestion agent IMPC_IMM_083_001 1.0 procedureMetadata Tick                         Collagenase DCollagenase II         TEXT
Cell digestion agent manufacturer IMPC_IMM_084_001 1.1 procedureMetadata Tick                         RocheWorthingtonGibco         TEXT
Cell digestion agent catalog number IMPC_IMM_085_001 1.2 procedureMetadata Tick                         #11088858001CLS2LS00417617101-015         TEXT
Cell counting IMPC_IMM_086_001 1.1 procedureMetadata Tick                         pre-lysispost-lysis         TEXT
Cell counting equipment manufacturer IMPC_IMM_087_001 1.0 procedureMetadata Tick                         Life TechnologiesAmerican OpticalBeckman CoulterBD BiosciencesMerck MilliporeOrflo         TEXT
Cell counting equipment model IMPC_IMM_088_001 1.0 procedureMetadata Tick                         Countess Automated Cell CounterReichert BrightlineGalliosBD LSR-IIScepterAttuneMoxi Z4468770         TEXT
Cell counting equipment name IMPC_IMM_089_001 1.0 procedureMetadata Tick                                         TEXT
Cell lysis buffer manufacturer IMPC_IMM_090_001 1.2 procedureMetadata Tick                         eBioscienceBD PharmLyseJaxJMCLONZA         TEXT
Cell lysis buffer catalog number IMPC_IMM_091_001 1.2 procedureMetadata Tick                         00-4300-54555899home brew10-548E         TEXT
Date and time of sacrifice IMPC_IMM_092_001 1.0 procedureMetadata Tick                                         DATETIME
Date and time of sample preparation IMPC_IMM_093_001 1.0 procedureMetadata Tick                                         DATETIME
Sample storage temperature until analysis (in Celsius) IMPC_IMM_094_001 1.0 procedureMetadata Tick                                         C FLOAT
FCS repository reference (URL/ID) IMPC_IMM_095_001 1.0 procedureMetadata                                                 TEXT
Balanced salt solution type IMPC_IMM_096_001 1.0 procedureMetadata Tick                         HBSSPBS         TEXT
Balanced salt solution manufacturer IMPC_IMM_097_001 1.0 procedureMetadata Tick                         SigmaLife TechnologiesWisentWakoGibcoBiochrom         TEXT
Balanced salt solution catalog number IMPC_IMM_098_001 1.1 procedureMetadata Tick                         D1408H6136-1L041-2021114190-144L 182-10HBSS 1X 14170-08814175-095         TEXT
RPMI manufacturer IMPC_IMM_099_001 1.0 procedureMetadata Tick                         SigmaLife TechnologiesJaxWakoGibconone used         TEXT
RPMI catalog number IMPC_IMM_100_001 1.1 procedureMetadata Tick                         R875811875-101home brew189-0214531800-022none used11875-093         TEXT
DNAse I manufacturer IMPC_IMM_101_001 1.1 procedureMetadata Tick                         Sigma         TEXT
DNAse I catalog number IMPC_IMM_102_001 1.2 procedureMetadata Tick                         DN25D8764         TEXT
Dead cell exclusion dye IMPC_IMM_103_001 1.0 procedureMetadata Tick                         Sytox BlueSytox GreenZombie NIRDAPIPropidium Iodide         TEXT
Dead cell exclusion dye manufacturer IMPC_IMM_104_001 1.0 procedureMetadata Tick                         Life TechnologiesBiolegendSigmahome brew         TEXT
Dead cell exclusion dye catalog number IMPC_IMM_105_001 1.1 procedureMetadata Tick                         S34857S-34860423106D9542S11348home brewR37606         TEXT
Automated analysis IMPC_IMM_109_001 1.0 procedureMetadata Tick                         YesNo         TEXT
Collection buffer manufacturer IMPC_IMM_110_001 1.2 procedureMetadata                                 Life Technologies         TEXT
Collection buffer catalog number number IMPC_IMM_111_001 1.2 procedureMetadata                                 24020         TEXT
FACS buffer manufacturer IMPC_IMM_112_001 1.1 procedureMetadata                                 Life Technologies         TEXT
FACS buffer catalog number IMPC_IMM_113_001 1.1 procedureMetadata                                 14175         TEXT
Enzyme buffer manufacturer IMPC_IMM_114_001 1.1 procedureMetadata                                 Life Technologies         TEXT
Enzyme buffer catalog number IMPC_IMM_115_001 1.1 procedureMetadata                                 14025         TEXT