Phenotyping Protocols for Pipeline: ICS ICS_001

(Adult)
Unrestricted
Housing and Husbandry IMPC_HOU_001 The housing and husbandry capture form is an institute overview form to capture how the animals ar... continue reading Protocol...

Experimental design IMPC_EXD_001 The experimental workflow capture form is an institute overview form to capture how the phenotypin... continue reading Protocol...

TickMandatory
Body Weight IMPC_BWT_001 The body weight test measures the weight of the mouse in a time series, allowing monitoring of its e... continue reading Protocol...

TickMandatory
Fertility of Homozygous Knock-out Mice IMPC_FER_001 To assess the fertility of homozygous knockout mice. To assess the fertility of homozygous knockou... continue reading Protocol...

Adult LacZ IMPC_ALZ_001 To stain adult mutant  and control tissues for bacterial beta-galactosidase (LacZ) activity in ... continue reading Protocol...

Viability Primary Screen IMPC_VIA_001 To assess the postnatal viability, sub-viability, and lethality of homozygous mice during cohort p... continue reading Protocol...

(Embryonic)
E9.5 and Younger
Viability E9.5 Secondary Screen IMPC_EVL_001 To assess the viability, sub-viability, and lethality of homozygous embryos at E9.5 ... continue reading Protocol...

OPT E9.5 IMPC_EOL_001 To assess morphological defects in E9.5 embryos from lethal strains with OPT. ... continue reading Protocol...

(Embryonic)
E12.5
Embryo LacZ IMPC_ELZ_001 To stain mutant and control embryos for bacterial beta-galactosidase (LacZ) activity in order to a... continue reading Protocol...

Viability E12.5 Secondary Screen IMPC_EVM_001 To assess the viability, sub-viability, and lethality of homozygous embryos at E12.5 ... continue reading Protocol...

(Embryonic)
E14.5-E15.5
Viability E14.5-E15.5 Secondary Screen IMPC_EVO_001 To assess the viability, sub-viability, and lethality of homozygous embryos at E14.5 or E15.5 ... continue reading Protocol...

Gross Morphology Placenta E14.5-E15.5 IMPC_GPO_001 To assess visible morphological defects in E14.5-15.5 placentas from lethal strains.   ... continue reading Protocol...

Gross Morphology Embryo E14.5-E15.5 IMPC_GEO_003 To assess visible morphological defects in E14.5-E15.5 embryos from lethal strains   ... continue reading Protocol...

(Embryonic)
E18.5 and Older
Viability E18.5 Secondary Screen IMPC_EVP_001 To assess the viability, sub-viability, and lethality of homozygous embryos at E18.5 ... continue reading Protocol...

Gross Morphology Placenta E18.5 IMPC_GPP_001 To assess visible morphological defects in E18.5 placentas from lethal or subviable strains. ... continue reading Protocol...

MicroCT E18.5 IMPC_EMA_001 To assess morphological defects in E18.5 embryos from lethal or subviable strains with MicroCT. ... continue reading Protocol...

Gross Morphology Embryo E18.5 IMPC_GEP_003 To assess visible morphological defects in E18.5 embryos from lethal or subviable strains &n... continue reading Protocol...

(Adult)
Week 9
Rotarod ICS_ROT_001 No description available yet

TickMandatory
Combined SHIRPA and Dysmorphology IMPC_CSD_002 SHIRPA and dysmorphology were originally always separate assessments.  However they have recent... continue reading Protocol...

TickMandatory
Combined SHIRPA and Dysmorphology IMPC_CSD_003 SHIRPA and dysmorphology were originally always separate assessments.  However they have recent... continue reading Protocol...

Open Field IMPC_OFD_001 The Open Field test is used to assess anxiety and exploratory behaviors. It is based on the natural ... continue reading Protocol...

TickMandatory
Combined SHIRPA and Dysmorphology IMPC_CSD_001 SHIRPA and dysmorphology were originally always separate assessments.  However they have recent... continue reading Protocol...

TickMandatory
Grip Strength IMPC_GRS_001 The grip strength test is used to measure the neuromuscular function as maximal muscle strength of f... continue reading Protocol...

(Adult)
Week 10
Fear Conditioning ICS_FEA_001 The fear conditioning is an associative learning paradigm for measuring aversive learning and memo... continue reading Protocol...

TickMandatory
Acoustic Startle and Pre-pulse Inhibition (PPI) IMPC_ACS_003 The acoustic startle response is characterized by an exaggerated flinching response to an unexpected... continue reading Protocol...

TickMandatory
Acoustic Startle and Pre-pulse Inhibition (PPI) IMPC_ACS_001 The acoustic startle response is characterized by an exaggerated flinching response to an unexpected... continue reading Protocol...

TickMandatory
Acoustic Startle and Pre-pulse Inhibition (PPI) IMPC_ACS_002 The acoustic startle response is characterized by an exaggerated flinching response to an unexpected... continue reading Protocol...

(Adult)
Week 11
TickMandatory
Indirect Calorimetry IMPC_CAL_001 Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy e... continue reading Protocol...

TickMandatory
Indirect Calorimetry IMPC_CAL_003 Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy e... continue reading Protocol...

TickMandatory
Indirect Calorimetry IMPC_CAL_002 Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy e... continue reading Protocol...

(Adult)
Week 12
TickMandatory
Electrocardiogram (ECG) IMPC_ECG_002 To provide a high throughput method to obtain Electrocardiograms in a conscious mouse. To provide a... continue reading Protocol...

Electrocardiogram (ECG) IMPC_ECG_001 To provide a high throughput method to obtain Electrocardiograms in a conscious mouse. To provide a... continue reading Protocol...

Echo IMPC_ECH_001 To assess the functionality of the heart in order to determine the presence of a mutant phenotype. ... continue reading Protocol...

(Adult)
Week 13
Challenge Whole Body Plethysmography ICS_CHL_003 The purpose of this procedure is to record the respiratory function of mice, with a LPS challenge, b... continue reading Protocol...

TickMandatory
Intraperitoneal glucose tolerance test (IPGTT) IMPC_IPG_001 The glucose tolerance test measures the clearance of an intraperitoneally injected glucose load from... continue reading Protocol...

(Adult)
Week 14
TickMandatory
Auditory Brain Stem Response IMPC_ABR_001 Auditory brainstem response test determines hearing sensitivity and other physiological parameters u... continue reading Protocol...

TickMandatory
Auditory Brain Stem Response IMPC_ABR_002 Auditory brainstem response test determines hearing sensitivity and other physiological parameters u... continue reading Protocol...

TickMandatory
X-ray IMPC_XRY_001 Construct and analyse digital X-ray images in immobilised mice using a Faxitron X-Ray system or NTB ... continue reading Protocol...

TickMandatory
Body Composition (DEXA lean/fat) IMPC_DXA_001 Measure bone mineral content and density as well as body composition in mice using the DEXA (Dual En... continue reading Protocol...

(Adult)
Week 15
Shock Threshold ICS_SHO_001 No description available yet

Hot Plate ICS_HOT_001 No description available yet

TickMandatory
Eye Morphology IMPC_EYE_001 To detect abnormalities in eye morphology. To detect abnormalities in eye morphology. ... continue reading Protocol...

TickMandatory
Eye Morphology IMPC_EYE_002 To detect abnormalities in eye morphology. To detect abnormalities in eye morphology. ... continue reading Protocol...

(Terminal)
Week 16
Immunophenotyping IMPC_IMM_001 Immunophenotyping of immune system cells This test differentiates immune cell sub-populations via f... continue reading Protocol...

FACS ICS_IMM_001 Immunophenotyping of immune system cells This test differentiates immune cell sub-populations via f... continue reading Protocol...

TickMandatory
Immunophenotyping ICS_IMM_002 Immunophenotyping of immune system cells This test differentiates immune cell sub-populations via f... continue reading Protocol...

Organ Weight IMPC_OWT_001 Organ weights from different tissues collected for IMPC To measure and record absolute organ weight... continue reading Protocol...

TickMandatory
Hematology IMPC_HEM_001 Hematological assessment of blood determines blood cell counts (white blood cells, red blood cells, ... continue reading Protocol...

TickMandatory
Hematology IMPC_HEM_002 Hematological assessment of blood determines blood cell counts (white blood cells, red blood cells, ... continue reading Protocol...

TickMandatory
Clinical Chemistry IMPC_CBC_001 Clinical chemistry determines biochemical parameters in plasma including enzymatic activity, specifi... continue reading Protocol...

TickMandatory
Clinical Chemistry IMPC_CBC_002 Clinical chemistry determines biochemical parameters in plasma including enzymatic activity, specifi... continue reading Protocol...

Insulin Blood Level IMPC_INS_001 The insulin concentration in the blood is an important indicator of diabetes.Ontological description... continue reading Protocol...

TickMandatory
Clinical Chemistry IMPC_CBC_003 Clinical chemistry determines biochemical parameters in plasma including enzymatic activity, specifi... continue reading Protocol...

Insulin Blood Level IMPC_INS_002 The insulin concentration in the blood is an important indicator of diabetes.Ontological description... continue reading Protocol...

Insulin Blood Level IMPC_INS_003 The insulin concentration in the blood is an important indicator of diabetes.Ontological description... continue reading Protocol...

TickMandatory
Gross Pathology and Tissue Collection IMPC_PAT_001 To perform a complete necropsy to detect and record abnormal external findings and macroscopic alter... continue reading Protocol...

TickMandatory
Heart Weight IMPC_HWT_001 To evaluate cardiac size using heart weight and body weight. To evaluate cardiac size using heart ... continue reading Protocol...

TickMandatory
Gross Pathology and Tissue Collection IMPC_PAT_002 To perform a complete necropsy to detect and record abnormal external findings and macroscopic alter... continue reading Protocol...

Histopathology IMPC_HIS_002 To perform histopathological examination and annotation on the standard list of tissues (see SOPs ... continue reading Protocol...

Tissue Embedding and Block Banking IMPC_BLK_001 Collect and fix a standard list of tissues from the complete necropsy (see IMPC Gross Pathology &... continue reading Protocol...

Histopathology IMPC_HIS_001 To perform histopathological examination and annotation on the standard list of tissues (see SOPs ... continue reading Protocol...