Parameters for Procedure: Immunophenotyping IMPC_IMM_001

Procedure carried out on Week 16

Version Type Req. Upload Req. Analysis Annotation Increment Option Ontology Options Derived Unit Data Type
Spleen weight IMPC_IMM_001_001 1.0 simpleParameter Tick                                         g FLOAT
Total number of acquired events in Panel A IMPC_IMM_026_001 1.4 simpleParameter                                                 INT
Percentage of live gated events in Panel A IMPC_IMM_002_001 1.7 simpleParameter                                                 % FLOAT
T cells (panel A) IMPC_IMM_003_001 1.5 simpleParameter                 Tick                         INT
NKT cells (panel A) IMPC_IMM_004_001 1.5 simpleParameter                 Tick                         INT
NK cells (panel A) IMPC_IMM_005_001 1.5 simpleParameter                 Tick                         INT
Others IMPC_IMM_006_001 1.4 simpleParameter                 Tick                         INT
CD4 T cells IMPC_IMM_007_001 1.4 simpleParameter                 Tick                         INT
CD8 T cells IMPC_IMM_008_001 1.4 simpleParameter                 Tick                         INT
DN T cells IMPC_IMM_009_001 1.5 simpleParameter                                                 INT
DP T cells IMPC_IMM_010_001 1.2 simpleParameter                                                 INT
CD4 NKT cells IMPC_IMM_011_001 1.4 simpleParameter                 Tick                         INT
CD8 NKT cells IMPC_IMM_012_001 1.5 simpleParameter                                                 INT
DN NKT cells IMPC_IMM_013_001 1.4 simpleParameter                 Tick                         INT
CD4 CD25+ T cells IMPC_IMM_014_001 1.4 simpleParameter                 Tick                         INT
CD4 CD25- T cells IMPC_IMM_015_001 1.4 simpleParameter                 Tick                         INT
CD8 CD25+ T cells IMPC_IMM_016_001 1.4 simpleParameter                 Tick                         INT
CD8 CD25- T cells IMPC_IMM_017_001 1.4 simpleParameter                 Tick                         INT
DN CD25+ T cells IMPC_IMM_018_001 1.5 simpleParameter                                                 INT
DN CD25- T cells IMPC_IMM_019_001 1.5 simpleParameter                                                 INT
CD4 CD25+ NKT cells IMPC_IMM_020_001 1.4 simpleParameter                 Tick                         INT
CD4 CD25- NKT cells IMPC_IMM_021_001 1.4 simpleParameter                 Tick                         INT
CD8 CD25+ NKT cells IMPC_IMM_022_001 1.5 simpleParameter                                                 INT
CD8 CD25- NKT cells IMPC_IMM_023_001 1.5 simpleParameter                                                 INT
DN CD25+ NKT cells IMPC_IMM_024_001 1.4 simpleParameter                 Tick                         INT
DN CD25- NKT cells IMPC_IMM_025_001 1.2 simpleParameter                 Tick                         INT
CD4 CD44+CD62L- T cells IMPC_IMM_028_001 1.2 simpleParameter                 Tick                         INT
CD4 CD44+CD62L+ T cells IMPC_IMM_029_001 1.2 simpleParameter                 Tick                         INT
CD4 CD44-CD62L+ T cells IMPC_IMM_030_001 1.2 simpleParameter                 Tick                         INT
CD4 CD44-CD62L- T cells IMPC_IMM_031_001 1.2 simpleParameter                                                 INT
CD8 CD44+CD62L- T cells IMPC_IMM_032_001 1.2 simpleParameter                 Tick                         INT
CD8 CD44+CD62L+ T cells IMPC_IMM_033_001 1.2 simpleParameter                 Tick                         INT
CD8 CD44-CD62L+ T cells IMPC_IMM_034_001 1.2 simpleParameter                 Tick                         INT
CD8 CD44-CD62L- T cells IMPC_IMM_035_001 1.2 simpleParameter                                                 INT
DN CD44+CD62L- T cells IMPC_IMM_036_001 1.3 simpleParameter                                                 INT
DN CD44+CD62L+ T cells IMPC_IMM_037_001 1.3 simpleParameter                                                 INT
DN CD44-CD62L+ T cells IMPC_IMM_038_001 1.3 simpleParameter                                                 INT
DN CD44-CD62L- T cells IMPC_IMM_039_001 1.3 simpleParameter                                                 INT
CD4 CD44+CD62L- NKT cells IMPC_IMM_040_001 1.2 simpleParameter                 Tick                         INT
CD4 CD44+CD62L+ NKT cells IMPC_IMM_041_001 1.2 simpleParameter                 Tick                         INT
CD4 CD44-CD62L+ NKT cells IMPC_IMM_042_001 1.3 simpleParameter                                                 INT
CD8 CD44+CD62L- NKT cells IMPC_IMM_043_001 1.3 simpleParameter                                                 INT
CD8 CD44+CD62L+ NKT cells IMPC_IMM_044_001 1.3 simpleParameter                                                 INT
CD8 CD44-CD62L+ NKT cells IMPC_IMM_045_001 1.3 simpleParameter                                                 INT
DN CD44+CD62L- NKT cells IMPC_IMM_046_001 1.2 simpleParameter                 Tick                         INT
DN CD44+CD62L+ NKT cells IMPC_IMM_047_001 1.2 simpleParameter                 Tick                         INT
DN CD44-CD62L+ NKT cells IMPC_IMM_048_001 1.3 simpleParameter                                                 INT
Total number of acquired events in Panel B IMPC_IMM_027_001 1.2 simpleParameter                                                 INT
Percentage of live gated events in Panel B IMPC_IMM_049_001 1.5 simpleParameter                                                 % FLOAT
Neutrophils IMPC_IMM_050_001 1.2 simpleParameter                 Tick                         INT
Monocytes IMPC_IMM_051_001 1.2 simpleParameter                 Tick                         INT
Eosinophils IMPC_IMM_052_001 1.2 simpleParameter                 Tick                         INT
NK Cells (panel B) IMPC_IMM_053_001 1.3 simpleParameter                 Tick                         INT
NK Subsets (Q1) IMPC_IMM_054_001 1.3 simpleParameter                 Tick                         INT
NK Subsets (Q2) IMPC_IMM_055_001 1.2 simpleParameter                 Tick                         INT
NK Subsets (Q3) IMPC_IMM_056_001 1.2 simpleParameter                 Tick                         INT
NK Subsets (Q4) IMPC_IMM_057_001 1.2 simpleParameter                 Tick                         INT
NKT Cells (panel B) IMPC_IMM_058_001 1.2 simpleParameter                 Tick                         INT
NKT Subsets (Q1) IMPC_IMM_059_001 1.2 simpleParameter                 Tick                         INT
NKT Subsets (Q3) IMPC_IMM_060_001 1.2 simpleParameter                 Tick                         INT
T Cells (panel B) IMPC_IMM_061_001 1.2 simpleParameter                 Tick                         INT
T Subset IMPC_IMM_062_001 1.2 simpleParameter                 Tick                         INT
B Cells IMPC_IMM_063_001 1.2 simpleParameter                 Tick                         INT
B1B Cells IMPC_IMM_064_001 1.2 simpleParameter                 Tick                         INT
B2B Cells IMPC_IMM_065_001 1.2 simpleParameter                 Tick                         INT
Follicular B Cells IMPC_IMM_066_001 1.2 simpleParameter                 Tick                         INT
Follicular B Cells (CD21/35+) IMPC_IMM_067_001 1.1 simpleParameter                 Tick                         INT
Transitional B Cells IMPC_IMM_068_001 1.3 simpleParameter                 Tick                         INT
Transitional B Cells (CD21/35 low) IMPC_IMM_069_001 1.2 simpleParameter                 Tick                         INT
MZB IMPC_IMM_070_001 1.2 simpleParameter                 Tick                         INT
MZB (CD21/35 high) IMPC_IMM_071_001 1.1 simpleParameter                 Tick                         INT
cDCs IMPC_IMM_072_001 1.2 simpleParameter                 Tick                         INT
cDCs CD11b Type IMPC_IMM_073_001 1.2 simpleParameter                 Tick                         INT
pDCs IMPC_IMM_074_001 1.2 simpleParameter                 Tick                         INT
RP Macrophage (F4/80+) IMPC_IMM_075_001 1.1 simpleParameter                 Tick                         INT
RP Macrophage (CD19- CD11c-) IMPC_IMM_076_001 1.1 simpleParameter                 Tick                         INT
Equipment name IMPC_IMM_077_001 1.0 procedureMetadata Tick                         FACSFortessa_1LSR IIFlow cytometer         TEXT
Equipment manufacturer IMPC_IMM_078_001 1.0 procedureMetadata Tick Tick                 BD BiosciencesBeckman Coulter         TEXT
Equipment model IMPC_IMM_079_001 1.0 procedureMetadata Tick Tick                 BD LSRFortessa Cell AnalyzerH47100123GalliosFACSAria IIIBD LSR-IICANTO-II         TEXT
CS&T Bead lot IMPC_IMM_080_001 1.0 procedureMetadata Tick                                         TEXT
Anesthesia IMPC_IMM_081_001 1.0 procedureMetadata Tick Tick                 Injection narcosis with Sodium Pentobarbital (Somnopentyl)noneInjection narcosis with Ketamine (100mg/kg)/Xylazine (10mg/kg)Injection narcosis with Tribromoethanol (Avertin)Isoflurane         TEXT
Cell digestion IMPC_IMM_082_001 1.0 procedureMetadata Tick Tick                 GentleMACSmanual         TEXT
Cell digestion temperature (in Celsius) IMPC_IMM_106_001 1.1 procedureMetadata Tick                         37RT         TEXT
Cell digestion agent IMPC_IMM_083_001 1.0 procedureMetadata Tick                         Collagenase DCollagenase II         TEXT
Cell digestion agent manufacturer IMPC_IMM_084_001 1.1 procedureMetadata Tick                         RocheWorthingtonGibco         TEXT
Cell digestion agent catalog number IMPC_IMM_085_001 1.2 procedureMetadata Tick                         #11088858001CLS2LS00417617101-015         TEXT
Cell counting IMPC_IMM_086_001 1.1 procedureMetadata Tick                         pre-lysispost-lysis         TEXT
Cell counting equipment manufacturer IMPC_IMM_087_001 1.0 procedureMetadata Tick                         Life TechnologiesAmerican OpticalBeckman CoulterBD BiosciencesMerck MilliporeOrflo         TEXT
Cell counting equipment model IMPC_IMM_088_001 1.0 procedureMetadata Tick                         Countess Automated Cell CounterReichert BrightlineGalliosBD LSR-IIScepterAttuneMoxi Z4468770         TEXT
Cell counting equipment name IMPC_IMM_089_001 1.0 procedureMetadata Tick                                         TEXT
Cell lysis buffer manufacturer IMPC_IMM_090_001 1.2 procedureMetadata Tick                         eBioscienceBD PharmLyseJaxJMCLONZA         TEXT
Cell lysis buffer catalog number IMPC_IMM_091_001 1.2 procedureMetadata Tick                         00-4300-54555899home brew10-548E         TEXT
Date and time of sacrifice IMPC_IMM_092_001 1.0 procedureMetadata Tick                                         DATETIME
Date and time of sample preparation IMPC_IMM_093_001 1.0 procedureMetadata Tick                                         DATETIME
Sample storage temperature until analysis (in Celsius) IMPC_IMM_094_001 1.0 procedureMetadata Tick                                         C FLOAT
FCS repository reference (URL/ID) IMPC_IMM_095_001 1.0 procedureMetadata                                                 TEXT
Balanced salt solution type IMPC_IMM_096_001 1.0 procedureMetadata Tick                         HBSSPBS         TEXT
Balanced salt solution manufacturer IMPC_IMM_097_001 1.0 procedureMetadata Tick                         SigmaLife TechnologiesWisentWakoGibcoBiochrom         TEXT
Balanced salt solution catalog number IMPC_IMM_098_001 1.1 procedureMetadata Tick                         D1408H6136-1L041-2021114190-144L 182-10HBSS 1X 14170-08814175-095         TEXT
RPMI manufacturer IMPC_IMM_099_001 1.0 procedureMetadata Tick                         SigmaLife TechnologiesJaxWakoGibconone used         TEXT
RPMI catalog number IMPC_IMM_100_001 1.1 procedureMetadata Tick                         R875811875-101home brew189-0214531800-022none used11875-093         TEXT
DNAse I manufacturer IMPC_IMM_101_001 1.1 procedureMetadata Tick                         Sigma         TEXT
DNAse I catalog number IMPC_IMM_102_001 1.2 procedureMetadata Tick                         DN25D8764         TEXT
Dead cell exclusion dye IMPC_IMM_103_001 1.0 procedureMetadata Tick                         Sytox BlueSytox GreenZombie NIRDAPIPropidium Iodide         TEXT
Dead cell exclusion dye manufacturer IMPC_IMM_104_001 1.0 procedureMetadata Tick                         Life TechnologiesBiolegendSigmahome brew         TEXT
Dead cell exclusion dye catalog number IMPC_IMM_105_001 1.1 procedureMetadata Tick                         S34857S-34860423106D9542S11348home brewR37606         TEXT
Panel A FCS file(s) IMPC_IMM_107_001 1.0 seriesMediaParameter                                                 TEXT
Panel B FCS file(s) IMPC_IMM_108_001 1.0 seriesMediaParameter                                                 TEXT
Automated analysis IMPC_IMM_109_001 1.0 procedureMetadata Tick                         YesNo         TEXT
Collection buffer manufacturer IMPC_IMM_110_001 1.2 procedureMetadata                                 Life Technologies         TEXT
Collection buffer catalog number number IMPC_IMM_111_001 1.2 procedureMetadata                                 24020         TEXT
FACS buffer manufacturer IMPC_IMM_112_001 1.1 procedureMetadata                                 Life Technologies         TEXT
FACS buffer catalog number IMPC_IMM_113_001 1.1 procedureMetadata                                 14175         TEXT
Enzyme buffer manufacturer IMPC_IMM_114_001 1.1 procedureMetadata                                 Life Technologies         TEXT
Enzyme buffer catalog number IMPC_IMM_115_001 1.1 procedureMetadata                                 14025         TEXT