Histopathology IMPC_HIS_002

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Purpose

To perform histopathological examination and annotation on the standard list of tissues (see SOPs for IMPC Gross pathology & Tissue Collection) using the tissue orientation laid out in the SOP for IMPC Tissue Embedding & Block Banking.

Experimental Design

Histopathological examination and description of tissues.

Minimum number of mutant animals: 2 mutant males + 2 mutant females

Minimum age of animals: 17 weeks

 

Notes

German Mouse Clinic:

Kleiner DE et al. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology 41: 1313-1321

 

Score

Grade

Definition

0

<5%

No lesion ( detectable)

1

5%-33%

Mild

2

>33%-66%

Moderate

3

>66%

Severe

 

TCP

0 Normal: Tissue considered to be normal, under the conditions of the study and considering the age, sex and strain of the animal.  Alterations may be present, which, under other circumstances, would be considered deviations from normal.
1            Mild: Observation is visible but involves a minor proportion of the tissue and the clinical consequences of the observation are most likely subclinical.
2 Moderate: Observation is clearly visible and involves a significant proportion of the tissue; it is likely to have some clinical manifestations that are generally expected to be minor.
3 Marked: Observation is clearly visible involves a major proportion of the tissue; clinical manifestations are likely and may be associated with significant tissue dysfunction or damage.
4 Severe: Observation is clearly visible and involves a major proportion of the tissue; clinical manifestations are likely and may be associated with significant tissue dysfunction or damage.

 

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Name Version Type Req. Upload Req. Analysis Annotation Increment Option Ontology Options Unit Data Type Derived History