The experimental workflow capture form is an institute overview form to capture how the phenotyping procedures are implemented. The questions have been based on the requirements of the Animal Research: Reporting In Vivo Experiments guidelines (ARRIVE)(Kilkenny PLOS One 2010), and Gold Standard Publication Checklist (GSPC) reporting guidelines (Hooijmans ATLA 2010).
Each unique set of conditions within a specified timeframe is submitted by centres. Each centre may have multiple sets of active conditions within the same time-frame if some animals have different experimental conditions depending on e.g. pipeline.
Submission identifier is used to identify a unique submission, and the same identifier is used if a set of conditions have corrected or updated details in a new submission, while new identifiers are used to identify each separate set of conditions.
The questions are based on the Mouse Experimental Design Ontology (MEDO) developed to describe experimental implementation.
This ontology can be examined at https://bioportal.bioontology.org/ontologies/MEDO
Some of the experimental conditions need to be specified for each procedure being performed. In these cases the parameters are series parameters where the three letter procedure identifier from the IMPReSS protocol key should be included as the increment name to identify the procedure and one of the defined options should be submitted as the value for each increment. For procedures where different conditions apply during data collection or imaging than during analysis, the stage in question should be specified after the procedure identifier e.g. "ABR collection" vs "ABR analysis".
This applies to the following parameters:
- IMPC_EXD_098_002 (Time effect strategy)
- IMPC_EXD_127_001 (Blinding)
- IMPC_EXD_128_001 (Instrumentation bias management)
- IMPC_EXD_129_001 (Operator effect control strategy)
- IMPC_EXD_130_001 (Order effect control strategy)
- IMPC_EXD_131_001 (Subject selection strategy)