Dark-Light Test CCP_LDT_001

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Purpose

To monitor the anxiety-related behaviour of mice in a two-chambered light-dark arena over a defined period of time in order to assess the anxiety phenotype.

Experimental Design

 

Procedure

1. Acclimatise mice for at least 1 hour before test.

2. Clean the arena with disinfectant and ensure arena is dry.

3. Light intensity levels are set at 200 lux at the centre of light compartment and at 5 lux at the dark one. Light adjustment is performed at the start of each test day.

4. The method of recording is set up with each arena defined. The dark arena constitute approximately 40% of entire chamber.

5. One mouse is placed in the dark compartment and recording is started.

6. The test lasts 5 minutes.

7. At the end of the trial, the mice are removed from the arena and returned to their home cages.

8. Number of transition and time spent in each compartment are calculated automatically by software.

Notes

Local data processing may be implemented to calculate derived measures from this data.

Data QC

Local QC data to assure all data fields have recorded data.  In the case all data fields have a value of 0 that particular mouse data will be removed.

1.       Verify the total time of each run

Parameters & Metadata

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