LOCUS       KO-first_condition_ready_45759_MGI:105982        23208 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Rfx1 targeting project(s): 45759
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 176745
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            5446..5784
                     /db_xref="ENSEMBL:ENSMUSE00000269634"
                     /rank="2"
                     /note="ENSMUSE00000269634"
     misc_feature    5434..11128
                     /note="5 arm"
     misc_feature    11156..11180
                     /note="B1 Gateway"
     misc_feature    11193..11240
                     /note="Frt"
     intron          11241..12115
                     /note="mouse En2 intron"
     misc_feature    12113..12115
                     /note="Splice Acceptor"
     exon            12116..12288
                     /note="mouse En2 exon"
     misc_feature    12300..12893
                     /note="ECMV IRES"
     gene            12894..15954
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15993..16236
                     /note="SV40 polyadenylation site"
     misc_feature    16240..16273
                     /note="loxP"
     promoter        16300..16819
                     /note="human beta actin promoter"
     gene            16845..17639
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17839..18080
                     /note="SV40 polyadenylation site"
     misc_feature    18097..18144
                     /note="Frt"
     misc_feature    18151..18184
                     /note="loxP"
     misc_feature    12130..12207
                     /note="FCHK std_gpr"
     misc_feature    18200..18220
                     /note="B2 Gateway"
     misc_feature    11129..18250
                     /note="Synthetic Cassette"
     exon            18631..18740
                     /db_xref="ENSEMBL:ENSMUSE00000269601"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000269601"
     exon            18893..18976
                     /db_xref="ENSEMBL:ENSMUSE00000269573"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00000269573"
     misc_feature    18251..19136
                     /note="Critical Region"
     misc_feature    19160..19193
                     /note="Downstream LoxP"
     misc_feature    19137..19216
                     /note="synthetic loxP region"
     exon            19851..19958
                     /db_xref="ENSEMBL:ENSMUSE00000269550"
                     /rank="5"
                     /note="ENSMUSE00000269550"
     exon            21384..21497
                     /db_xref="ENSEMBL:ENSMUSE00000269524"
                     /rank="6"
                     /note="ENSMUSE00000269524"
     exon            22818..22910
                     /db_xref="ENSEMBL:ENSMUSE00000269502"
                     /rank="7"
                     /note="ENSMUSE00000269502"
     misc_feature    19217..23208
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggatttctac tcaaggaaga ggaaaaccgc
     5461 cttatttatt atcattcact gttggcatgg caacacagtc ctatgttact gagctacagg
     5521 cagccccaca agcatcccag ccgccacagg ccccgcccca ggccctgccc cagccaccac
     5581 ccccagcagc accccagcct cctgctgcag caacccccca gccccagtat gtcaccgagc
     5641 tgcaaagccc ccagccccag acgcaacctc cgggcagcca gaagcagtat gtggcagagc
     5701 tccctgctgc ccctgcaccc tcacagcctg ccacacctgc cccatcccct gtggctcagc
     5761 agtatatcgt ggtcaccgta tcgggtaagt tcacatctgt acttgtggcc agagtgcttg
     5821 gaacatctct gggaggagcg tggggagctc agcatccagg gatccgctcc agggctacct
     5881 gtgcaagcga atcaaggcat ctctgggtaa agggactcgg gccaagcaga ggagcttctc
     5941 atcctaccgg aatgcttctg tgtttatctg tccccaagta aaatgtcctc agaaaagagg
     6001 atgggatggt gaaattcagt ttgcacacac ctgcaagtgt caggctgccc actgaccctg
     6061 cgcctctctg cctaggtctt accattgcag cttctctaaa tgaggacatg tgagacgtgc
     6121 ttagagcagg tcgacccgga agaggtcctt gagagatgct gtctagatgg ctgagatgta
     6181 ttctctctac tccctggagg agcaggacct ggtggccaag cacactcctg agcccatggc
     6241 taacagtgta gcatggaaaa ggtctgattt aatagagctg agtctaatgt catagcctga
     6301 ggatgtggac aagggaaggt cagccacagc tgaaggtccc tctggggtgt caccacaggt
     6361 ctccagcctc tgcccctctt cctcttccct tcctgggaag cagaggaagg gggcagaagc
     6421 tgcactcacc ggaacagcca gtgtgacacc tgcactctct ctctgcccca cctgagcctc
     6481 tctgacctta gtgggatcag gtcctggcca gactgtgcct acgactcctc agaggtggag
     6541 tgatcccacc tgccccagct cttctgcagg ggcctgcaac tcctgcccgg aatactcttc
     6601 tttgttttgt ttttgttttt gtttttcttt gtcatatgtc catagactac tagctctgtg
     6661 attactgatc tctcatgtcc acccagttgg cagccacctg tgaccttgct cctgacagct
     6721 cccaccaggg ttcccatcct ttcagaatca tgggtcatcc tgggcctttg ttactcttgg
     6781 cctcgaggcg gtgcctggcc ggcttcctcc tacctcagac ctgtcctaca cgcctgccca
     6841 gctgccatca catctgcctt ttttgttgcc tccactgtct tctgcctctg atccatagca
     6901 gcaaagggct caggccctgc cctgctccag tcaccgtcct cctgatggcc tcctgcagtg
     6961 tcatggctct cactccttcc tttctcccag tactggggtt tgaactcaga gcctcacgca
     7021 ctctagcccg ctgctcttca gcgagctcca tccctaactc tctctttact cttatttgag
     7081 acagggtctc atcagctgcc cactctcacc gagctcactc tgcgcagcct tgcctccttc
     7141 accttgtcag ccttttcctt cagcctcttc agaagctggt gacaccagac ctaactgtca
     7201 aacttgccat gtgcctgttg gggtctccac cctagcctct gccctgagcc ccaggatctt
     7261 gtgacctcag gatcttgtga ctagcccacc caggtgtggc tactgaaggt catcttacgc
     7321 cctctcccta caggctctat gagatgtccc cttccaccca ggcccagctt agcctctgct
     7381 tctgtcctgc ttctccagat gttgctattt cgccccactc cctccttcct aaacctaccc
     7441 agaccctcta cctctaccac gtccatgcca tctctggtta gctccgatgc cttattctca
     7501 cagtagccac aaggagcctg tccctagctg ctacttaggc ctcttcacat cccgtctgcc
     7561 acgattccag agcagggctc tagggcatag gagtttgcag tattagaatg ctggccccta
     7621 tccctcccag aaaagcaccc cttaacacct gtaatcacac tgggggaggg ggttgtggct
     7681 ggaggagtcg aattccaggc taatctgagc tataaatcaa gatcccatct caaaaaaaaa
     7741 tttttctgtc atcattttct gaataagctc caagtgcctg tgttttgccc agcagctcct
     7801 agccctgtgc ctaacatgac tgagcctccc cagcctcctg ccatgccagc tcaccagctt
     7861 agctacttct ccttttacct tggcacctac cacctcaagc atacccaggc taatctttct
     7921 gctaccttca tctccagtct ctcctcagaa gccacttccc tgccctctga tcccaccatc
     7981 catccttttc ttagcatatg gtagtgatgt ttttaaagac agggttctgt ctctaggttc
     8041 tgtgcaatga gtggtggata taggcctggc ccagcacaac tagctgaggc catctagcct
     8101 agcatcccat tgcacttgag tgacccacca caagttcctc ctgcaagcat ttggtgtcct
     8161 cagagtagaa tctggaatag gcacactgtt ctaggccctg cctcagcttc tcctagctct
     8221 tcatgacaca ccaagtgaag acctggtatt caaagtcccg ggccagagtg gcatttcagt
     8281 cagtcaggcc taggtcagtc tcactagctg tggggtggag atatggcatg ctatcagggg
     8341 acaaagcaca ggtctgggtt ggctgcagct cttgtctgca gtgtcactct gccaggctca
     8401 cacctctggt tgggcagtgg cttgttgctt gctttttacc atggttctgg gaatgccatc
     8461 ccactgagtt ctggcaggag tgagcccagc cctgcaggtg tcactgttac ccccatgagt
     8521 gctaaggaag aggagggagg ctgctaccca cccagcttcc gtgttggtgt cttcctactc
     8581 cctctcccag gctgcccttg tgagccagtc ctctgagaaa ggggaggctg acactggaga
     8641 ggatgaacag ggaggtacct aggccagctc cagagccagg aacaatctga gctcaccaag
     8701 aaggctgcag gggaaggact gcccgtaccc ctagaggagg cagcaggagt aaactgagac
     8761 ctctagcctg gtccggccac gctctgccct aaggtgacta gagatgatgg cagggagatc
     8821 aggtttccct ggtcagatga cacagagcca taatcctgag ctcccctgag gcctctagaa
     8881 cctactccat aggctgggga ggtgaagcca gacctgccag agggaccaag gagcctgcag
     8941 gagacactag ggccaccaag gcttctctgc tttgctttag cttgtggtcc cccaaaggac
     9001 ttgtgcctct gcaggagcac tgtgctcaca agaggaaaat gggagctgct ggtcagcgtc
     9061 tccagggaag aaggggaagt aaggtccaag aatccatgag tcctgggctt agtaggggcc
     9121 cagaatcctt gtcctgatgt ccatacccag gagcaaaaag aggtcctggc tggcgagaat
     9181 gcctcctgga gagctggctt gctctcttta agctgagggg tgaacaggtg gaggtggaga
     9241 agccaggcac ttgaaggaag gggtggccac atggagatag gctgtctgat gcaaacagga
     9301 cattagctct gttcctaact ccagctttcg ggtccccctg cttatgcaca cacccagaag
     9361 agaattggcc aacagataca gctgtcccta gactctactt tcccctcctt tcctcccagg
     9421 tcacctttca aaacatagct agcattccct ctcctaccta gggttggtgg gtagggaaga
     9481 gcccagcatt aagggccacc ccaaggcaga tgatagcagg attgcttcta ggatagatgc
     9541 ctgattagag cagatgccca agtgtccagc tgcccgggtc tggctcaagg gccagcccct
     9601 tggacattgc aggtggccag ggccaatgtc tgtgtagctt cccacccgga gccatagcag
     9661 ttccccgtgg gtctcctcag tccagtgccc atcctcgagc tgggcctggc tccctgtatt
     9721 ccaggtagcc cagttactgg ggggaaggag agaggatgag acctgtgact ctaggccagc
     9781 ttgattttcc ctcagagggg ctgagggaga gggccttctc tgggaacctg agaagactaa
     9841 agcagttttg agggaagatc ccagcaaaac ctaattgatt atcactgctt agaattggtg
     9901 taggaccaag caggcctggg aaggccacag gaagtcagtc tctctcccct gacagctctc
     9961 ccatgcctgg ccatttctgg ggcatattca gatcctctca ggcgactgag ccagcctcct
    10021 gccctagcca gccccttttt ctgtgtccct cctggaagct gagccctatt atggaaacct
    10081 caacgatatg tgtctttgag cgccccagtt ccctgtgcca gtctgtttga ctctgcctaa
    10141 taagatcagc aacttagaga tggtgtatgg ctgggcatga tggcatatgc ctttaatccc
    10201 agtacttggg aggcagaggc aggtggattt ctgagtttga ggccagcctg gtctacagag
    10261 tgagttccag gacagccagg gctacacaga gaaaccctgt ctcgaaaaac caaagggggg
    10321 gggggaagaa atggtgtatg gagttgggtg atgtgggcat acagggaacc cctggaccca
    10381 ggctagcccc ccccctcagc tgggtgatac gagggctttg tcacctgaaa atgaatgaga
    10441 aaggcaagta ccagctcaag gggcccaggt ccccctagag tgtcacatgg cacaccagaa
    10501 acagaatcag tgttcactgc cgtccctttg attaccctcg ttgccatgta gccctttagg
    10561 agatcaggtg acctctctgg acctctacct ttaccccctg ccagatccca gaaagatggg
    10621 cctggggcct tggttccttt aggcagtact ggagaatgag aacaggctca tggctacccc
    10681 tcctgacctt ggcccctgag ctactgagga actagttagg ctatatgtag gggtaggcca
    10741 gataacttcc cagcttgagc aagaatctcc tggccatttg tcttgggggc actgtccctg
    10801 cacacttctc tgctgtccca tgaatcacgt agcagtatgt gtataaaaat gagacctgcc
    10861 tctgatgata gcatctctgg gtattagccg tcctcttgtc ctggccccag gcccctcagc
    10921 agcccgaatg aagcagattg tttatctaag agggcttgaa ggagttctga gccatggtag
    10981 ggattagttg tccaaggtgt tgggtgtaaa gcctcctgta cactctgctg tagtgctagg
    11041 ctggctcctg cgatggatta ggagcaggca tgggcaccat gttctaagat tgtctcttgg
    11101 ctgcctgcca gcataagtac ctgcacttaa ggcgcataac gataccacga tatcaacaag
    11161 tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct
    11221 ctagaaagta taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca
    11281 ctgccaacta tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct
    11341 gttgggaggg tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag
    11401 gtggtcccaa ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc
    11461 ccctatccca taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag
    11521 agactcagtg aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca
    11581 ccaggcctca gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt
    11641 gtgggttaca taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc
    11701 agtcttctcc atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa
    11761 agataagggg agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc
    11821 cgctagcttt tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc
    11881 catctcattt tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc
    11941 ctcaagttta tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc
    12001 ccccccccac ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa
    12061 gtagctagac tccagcaacc agtaacctct gccctttctc ctccatgaca accaggtccc
    12121 aggtcccgaa aaccaaagaa gaagaaccct aacaaagagg acaagcggcc tcgcacagcc
    12181 ttcactgctg agcagctcca gaggctcaag gctgagtttc agaccaacag gtacctgaca
    12241 gagcagcggc gccagagtct ggcacaggag ctcggtaccc ggaagatctg gactctagag
    12301 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
    12361 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
    12421 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
    12481 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
    12541 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
    12601 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
    12661 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
    12721 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
    12781 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
    12841 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca accatggaag
    12901 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    12961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    13021 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
    13081 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
    13141 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
    13201 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
    13261 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
    13321 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
    13381 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
    13441 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
    13501 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
    13561 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
    13621 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
    13681 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
    13741 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
    13801 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
    13861 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
    13921 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
    13981 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
    14041 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
    14101 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
    14161 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
    14221 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
    14281 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
    14341 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
    14401 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
    14461 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
    14521 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
    14581 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
    14641 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
    14701 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
    14761 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
    14821 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
    14881 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
    14941 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
    15001 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
    15061 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt
    15121 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
    15181 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg
    15241 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg
    15301 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg
    15361 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga
    15421 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
    15481 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc
    15541 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
    15601 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
    15661 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
    15721 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac
    15781 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
    15841 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
    15901 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
    15961 cgggcagggg ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
    16021 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    16081 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    16141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    16201 ccaaactcat caatgtatct tatcatgtct ggatccggaa taacttcgta tagcatacat
    16261 tatacgaagt tatgtttaaa cggcgcgccc cggaattcgc cttctgcagg agcgtacaga
    16321 acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
    16381 agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc
    16441 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg cgcgggggcc
    16501 acggcgcgcg cccccagccc ccgggcccag caccccaagg cggccaacgc caaaactctc
    16561 cctcctcctc ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga
    16621 gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat
    16681 cagcgtgcgc cgttccgaaa gttgcctttt atggctcgag cggccgcggc ggcgccctat
    16741 aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga
    16801 gcctcgcctt tgccgatcct ctagagtcga gatccgccgc caccatgatt gaacaagatg
    16861 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
    16921 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
    16981 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
    17041 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
    17101 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
    17161 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
    17221 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
    17281 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
    17341 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
    17401 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
    17461 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
    17521 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
    17581 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
    17641 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
    17701 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    17761 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccccggatct
    17821 aagctctaga taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc
    17881 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    17941 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    18001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    18061 atcttatcat gtctggatcc gggggtaccg cgtcgagaag ttcctattcc gaagttccta
    18121 ttctctagaa agtataggaa cttcgtcgag ataacttcgt atagcataca ttatacgaag
    18181 ttatgtcgag atatctagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    18241 cagtaggcgg cactgtgttt cctaaggagc caggagaagc tcagcacttg gtccttcttg
    18301 agctgccaag acttgagcaa gaaagtgagg atccagtcta ctgtctgtcc atgtccatgg
    18361 ttctgctttc gtgggaaaga ggctcctcag acatatttca gaacagagag atcaggggct
    18421 aattccatat gccaagggtc ccaggtcctc tgcatgctca ctccatcttg ccatgtacct
    18481 ccgacagatg ctagagaagg ccaggcagga cttggagaag ccaggtctcg agggtggact
    18541 gtaggctgca ttgccctctg ctgacgggct aactcttgga atagagacag agacggtagg
    18601 cagagctgac ctgccaacct cttcccacag aaggtgccat gcgggctagt gagactgtgt
    18661 cagaagccag ccccagttcc acggcaagcc agaccggtgt tcccacccaa gtggtccagc
    18721 aggtgcaggg cacccagcag gtaggacatg gccttcactg ggggaagagg ctctcctcca
    18781 ccttaccatg aacatctgtt tacctgtctt atcaagatat ggcatcagcc acctccaccc
    18841 gcccatcctg gtaagcaacc ctcattcaac tcctttcttc ttgcctccac agcggctgct
    18901 ggtccaggca agtgtgcagg ccaagccagg ccatgtgtcc cccctgcagc ttaccaacat
    18961 tcaggtgcca cagcaggtaa ggctcctggc tccagcctcc tgtggggcag agaactactt
    19021 ccccactgct tgcttgtgtc tgttcttgcc agagtcccag gcaagggtat catcctcagg
    19081 ctgcctcagc tcctgactct gggcagtggc ctctagaggc aagattaaat accctagaga
    19141 tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga
    19201 gctcgccatc agttcacaag gtctggtgct ggtgcccttg actgctctag tctctcagct
    19261 catatttgga ctgtgctaga atgctgggac ccagcaatgg ggcataatta acatgtgctc
    19321 tgtcaccaag ctccattcca gcccagtgct aacagactgt aaggataaga acgttcctct
    19381 cctgtaacag gggatgtttt gagatagggt tttctatggc ctgaaactcc agatataatg
    19441 gagtatggcc ttgaactccc gatctgagga gtgcaagcat gcactgacag tgggctctag
    19501 catacattcc ctgccttctg agccaggagc cagctctggc tttgctgctt cttggcttca
    19561 gggctcaaca agctccacat cagctttgag cttgtgtgtc gcctgctatg gagtagagcc
    19621 ataactgtgc tgtgttgggt gcagggcagt tcctgggctg acaggcatgg gtgagcctag
    19681 aatgttcctg gtgcataggt gcttactgaa atgcagaaga ctggaggggc tcgttagtgg
    19741 gatgatatgt attagggcct ggttccatcc atagcatgga aaaacaaaac tcgggacttg
    19801 gctgtggctg gtgctgctac tgaccatgct cctttttctt ttgaccccag gctatcccca
    19861 cacagcacct ggtggtgcag agcccagcac cgggcactaa gagtggccag gtctccctga
    19921 cggtgcacag tgcccagcag gtgcactctg ctcctgaggt aagtgtgcct cactgacgtc
    19981 gtcccatctt tctctgttct agccatgggc cccagcagca tatttgtccc ccatgacctt
    20041 tttttgaggc agggtgttac agtgctatgc aggctagcac caacctccta tgtggagcag
    20101 gctggtctct accgtgagct cctcccgtgt tagtcccgag tgctgggtgg caggtgtgta
    20161 ccgctgtgcc tggcttcagc tggagctctt ttgccctgag ttcacttgca gctcctgagt
    20221 cctacaccct gctccagtga ctctgggtgg gtttcccagc taatgtctgc ctggccctgc
    20281 catcccagcc gatgttgaga gagctgtagt ccggaagtaa aagagttttc tcttccagct
    20341 tcagagtgaa tagagtccca tcatgagagc ttggaacaca cacacacaca cacacacaca
    20401 cacacacaca cacacacaca cacacacaca cgaatgagga gagcctggag gcagtgggct
    20461 gcagatggtc ttgagcttag attaagagtg cagggccaga cagggccctt taatcccagc
    20521 atttgggaag cagagactgg aatctccatg agattccata ttgagttcca ggacagtcag
    20581 ggctattaca tagtgagaca ctttctctaa ataaataaat aaataaataa ataaataaat
    20641 aaataaataa ataaataaat aaataaataa gaaggaaaga aggaaggaaa aataatacag
    20701 ggccagatgc caggcttagc tgagtctttg ggcaggtctc cagcattata gcccagtatt
    20761 tcctgtgtat aacagagcta atggcactgg gttgccacac atctgcatac agaggctgaa
    20821 gcaggaaggt ttcaagtgtg gtgctaaatt gcattttata gagagaccct gtctcaggaa
    20881 gaactagtaa aacccatgat tcatccactt gctcacatca gaattgcagt ccacactagt
    20941 gcagcatgcc ctagagcaag cacaggctca ctgtgtggcc ctgggcatct ctctctggga
    21001 aatgggggtc ttgaagcatc cattgcagca agcagtgggg gcagcaagag gtgaaagtag
    21061 attcagtgtt gttactactg tcccattggg aagcctggca ggatcaggag gagctgggtc
    21121 acgggaccag actagctccc agcctactct ttctcacctt tgctgcagct cttctgatat
    21181 agaagctaca agtggcctct ggccctcatg gaggctgaga cacctggagc cccatctagg
    21241 tagagtcctg cagagcagta tttgtggctg ctgtgggcta tcccaccctc tccagagtcc
    21301 ttctgaggca ggttgttaga gcctgggtgc cccagacaca agcacagctg gccctgacat
    21361 tgtctctgtt tgttctctta cagaggtcac cagtgcaggc caacaactcc accagcaaga
    21421 cagctgggac tcctgcggcc actgtgcagc aactacaggt ccacagcgtc cagcagagtg
    21481 tccctgtcac ccaagaggtg cctgaccagg gcgggcagta gctagggcca tgggtatagg
    21541 ccaagtagcc acctatgtct gggtccctgg cctgtgggag ctaagcagat ggacccagct
    21601 ccagcctacc atgtgagcat ttgacgagag cctctctctg cctctatcta gattcagcgt
    21661 ccctagttgt gacagttgta gaggtgcctc acagggcaca ttatggcttt gaaaacacac
    21721 actggtaggc cagagagatt gctcagtggg taaagcacac ttgccaccaa tcctgataac
    21781 tgagttctga cttctacaca cacaaacaca cacacacaca cacacacaca cagagacaga
    21841 cagacagaaa agaaaagcaa agcctgcagg gtgagcattt gcccatcata cacaggccct
    21901 gggtgtcatc tcttttctgc aaaaccaaaa agccagcttt ctggggagaa gaagcaccaa
    21961 ggccttcaga ttttacatct gttttaattt ttcttcagat tttacatttg ttttaatttt
    22021 tttttagtga gcatgggtgt tctgcttgca tgtgggtcta tgcaccatgt ttgtgcctag
    22081 acgtggagtt aaagctgtgt cggtgctagg aattgaacct gggtcctctg gaaggacaac
    22141 cagtttcctt aaccacagat ccatctctta agcctgccat tctcacgatg tgctcaacct
    22201 tccaaaggag actgagtttc ctaataccat cacagtggcc tgagcaggcc aagatgcaag
    22261 gatggtcttg tatctccttg ttaactgctt aactcttggg attcttggct tttcaagaaa
    22321 acaagacatg gtttgtgtgt cctcatagcc tcaactacaa gagactcagg ccagatcccc
    22381 acttcaaggc cagtctggac aatgtagtga aaccctgatt cagaataaaa attgaaaaac
    22441 agtgctgggg tgtagattag tggtagagag ctggcctagc atgcataaga ccccaggttc
    22501 cccagtacca ggctgaaaac ctcaaatagg cccgagctgt aggctcttcc ctagaggaaa
    22561 tgggaacctt ctaattcaaa ggatccacag gtattccctt cccgtgcaag aaccactctg
    22621 aacctccaga tggcagaggc aggcagtaag agacctggaa ggcctgtatt aactggaccc
    22681 cacacggaaa ggctgctctc tgtctgaaga atgacagcct ttcccaagac ggctgttctg
    22741 agcatgcatg ggacaggctg ccctagttgc catggttacc atactcgaac ttgcctctcc
    22801 ttgcccttct ttgccagagg tcagtagtcc aggccactcc acagaccaaa gctggcccgg
    22861 tgcagcagct gactgtgcag ggactgcagc cagttcacgt tgctcaagag gtgcgtgtcc
    22921 tatctgtcta tgtcggggca atcagactag tcctgggctg tggctggagg tacaggcacc
    22981 taatccagag ggaggaatgg agccagctgg ggaccctaaa gggcagacag tgggggcaat
    23041 tgaggtttga agccctgcag aagtgaggtt tcactctagc cctcggcttc agacttcatc
    23101 ctgaatccgt ccccatccca gcacctgacc actcaggaca caggtcttgt tgaaagaggg
    23161 ctggattcga attccgcttc tttctgatag tgcccagttc gttcctct
//