LOCUS       KO-first_condition_ready_33835_MGI:104716        21843 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Nnat targeting project(s): 33835
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 44045
COMMENT     backbone: R3R4_pBR_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     misc_feature    161..3098
                     /note="pBR322"
     CDS             complement(2030..2890)
                     /note="AmpR"
     misc_feature    3109..3232
                     /note="R3 gateway site"
     misc_feature    3238..3245
                     /note="AsiSI"
     misc_feature    1..3270
                     /note="Synthetic Backbone"
     exon            7454..7604
                     /db_xref="ENSEMBL:ENSMUSE00000552733"
                     /rank="1"
                     /note="ENSMUSE00000552733"
     misc_feature    3271..8111
                     /note="5 arm"
     misc_feature    8139..8163
                     /note="B1 Gateway"
     misc_feature    8176..8223
                     /note="Frt"
     intron          8224..9098
                     /note="mouse En2 intron"
     misc_feature    9096..9098
                     /note="Splice Acceptor"
     exon            9099..9271
                     /note="mouse En2 exon"
     misc_feature    9283..9876
                     /note="ECMV IRES"
     gene            9877..12937
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12976..13219
                     /note="SV40 polyadenylation site"
     misc_feature    13223..13256
                     /note="loxP"
     promoter        13283..13802
                     /note="human beta actin promoter"
     gene            13828..14622
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14822..15063
                     /note="SV40 polyadenylation site"
     misc_feature    15080..15127
                     /note="Frt"
     misc_feature    15134..15167
                     /note="loxP"
     misc_feature    9113..9190
                     /note="FCHK std_gpr"
     misc_feature    15183..15203
                     /note="B2 Gateway"
     misc_feature    8112..15233
                     /note="Synthetic Cassette"
     exon            15654..15734
                     /db_xref="ENSEMBL:ENSMUSE00001309983"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00001309983"
     exon            15983..16963
                     /db_xref="ENSEMBL:ENSMUSE00000680629"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00000680629"
     misc_feature    15234..17156
                     /note="Critical Region"
     misc_feature    17180..17213
                     /note="Downstream LoxP"
     misc_feature    17157..17236
                     /note="synthetic loxP region"
     misc_feature    17237..21843
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
      421 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
      481 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
      541 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
      601 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
      661 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
      721 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
      781 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
      841 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      901 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
      961 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     1021 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     1081 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2341 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2641 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3061 aaaataggcg tatcacgagg ccctttcgtc ttcaagaaaa ttgtcgacca tagtgactgg
     3121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3181 ttgatattta tatcatttta cgtttctcgt tcaactttat tatacaaagt tgcggacgcg
     3241 atcgccggcg gataacaatt tcacacagga taagatatgc tgggtggatg acgagcctta
     3301 tttatttttc caactgaaat gccacgaggg gtttcactca tttttacttc aaaagcagca
     3361 tcataaaaat ccttgacgat tagagcccca gctgtcccaa gttgtttatc gagggatcca
     3421 ctcctcaagt cctatagagc agacagcttc aggcaggaag ccatgggagc tgagaagtgc
     3481 aaatggcatc tctgaaaact tccagactcc ctcctgttaa tgcctgagct gccccctgct
     3541 ttcctcccca attccagcat ctgttctcca gagaacagat cagattttcc agctaaggta
     3601 ctctggcgcc aaggactcag ccctgtgtgc ttcaccctgc cccccaccaa ctcagaaatc
     3661 caacaccatg acccaaatgg actgcagcca cataaaacca caaagtaaag tttataaagc
     3721 tttattaaac atttcaaaca gctgtggaac gaacacacca gaaataaaag ctttacaaca
     3781 gcagtccgag tgtttcacaa gtgtggcctc taagtcccac tccccagaag cctgcttcca
     3841 gctctgtcct atgcctgccc ccccccccca cttgcacaca agagggggaa aggagggggg
     3901 tacatttccg actggaagaa caaccatgct gatccccagt gggtaggggc caggagaaag
     3961 tctcatttgc aaacacttgt ttctgaagcg cagagaaaag caaacaagta ccaagtcacc
     4021 aaatctttct tcctgtgttt tcttcatagt gtacagtcta cgtgcgcagc aatgaggaag
     4081 ctcctggtgg gaggatgatg gcgactgctc ccctgggtct tgtctgaacc accttggagt
     4141 ccactgtgct ggtcgctctg caatccccat gctaaataaa aactgcagca ggacctacta
     4201 cagctagcta ctactactaa acctcaaaat cagaacatat tcaataaagc ttcttcaaaa
     4261 ggaatataca catgtattgg tatgtaaaca cacacacaac acacattcac acatacgtgt
     4321 gtacatacac gctgacacta gtgcacacat acatacaggg cacatcaaaa atatccaaac
     4381 acctaccaag ggaatcatgg ctgctatgaa aaatgtaata ctttctcatt cactcagcca
     4441 gggaccactg ggtctacgag aatactgcac atctccctcc tgaagagcat gcactggaga
     4501 catgaggtgg cccctttagg tcagctgaga gagacaggag gaagagcaaa gggagagagg
     4561 ctgggctgga aggcaggagc ccagataccc aggccaaaga gcaatgtctc cccatcgctt
     4621 ctttcttaaa aatgaggcag aggaaatact ggaggtctgc agacaacgag cagttgagac
     4681 atgattccgt ctcaccttct caaaagggaa cgaaggatga aaaagcgagg ctctcgggca
     4741 tcagcaatct cttcaaattt gagataagtg caaaaacaag ccctgtcttg gacaggtgct
     4801 ccccaaaaaa ggaaaggggt tggaagtgaa ccaaggggaa aacggggaaa ggggacgagg
     4861 agggaacgag gaaggtcttg acgaggaagg gttatggagg tgacattgag actacactgt
     4921 atccacacag gtcatatcaa gggtatagat tccccaaatc aatgagcaag ggggtgggcc
     4981 aatccgtcag ccgggaccca gatggtgaca gaacacacaa ccataagacc acccacgact
     5041 acaggactct acaacagaag caccgatcag tgccattatt cacattgtaa ggataaaaca
     5101 tcacaggcca aaaggctcca ttaggaatgt ggtacccgca ggactgcggg atttgaaact
     5161 ccaatgcttt atgacctatg tcaatgcctc ccctcccgtc ttctgcttcc ttggcaagct
     5221 atctcggcag acgttaggtg cccacaacac cggggtcatg ggctgattcc ggaagtgggg
     5281 aatcggagca gtggtacagg aaacaatttc cccagcagct atagcagatg agaaagaggg
     5341 ctgccaggaa aaccaaggca catattgtgc aaggtttccg ttccaggagg aagctgagca
     5401 ggtagaagcc catgaacatg gagtggctga accacagagc ggggttgagg ggcttgggga
     5461 tgaggaggac gggcagcagc cactggaggc aatacatgat ctctgccggg cagggccttc
     5521 accaaggctg ccaggcaccg ctgcaggaac cgacctaatg ggagccagca ggcacaggtg
     5581 gctgcccttc gctttcttcg actctctctc caggagctga gccgctgtgc tctctggctg
     5641 tcagcccggg atcaccaagg cagcagggat cctgaagaga aaagtcaaca caacaaacag
     5701 acaaaggaca gacagacaca cacaaaaaac aaaaaacaac aaacaaaaca aacaaaaaaa
     5761 caggaacaaa ttgtatactt atttgggaaa atggatcatg gttaacaaca gcaaatgtga
     5821 atgccctttc ctccccaccc acccatgaat gtctactcct atccaaatta ttacttaaag
     5881 atagcctgct agggtgtgtc ttccctaggc ttctactggg ggatcccagg aggacacggt
     5941 ccccacagtg ggctggcttt gcctgggccc tgcccagcag agatcatgta tcgcctcctg
     6001 gggctgcagc ccgtcctcaa aggtcccaga cctcacaata ccgccctgtg gctgccacag
     6061 gacaccatgg gcttggtgtt gtgccaccgt gtggtaagga aaggctctgt atctagtctc
     6121 acatcttgct gctactgagt gtggtggagt atgccaattt tacaacagtt atctgtgcac
     6181 tgccgacctg gagaggctag gcaatggaac ctcagatcct ggcaagctat gggaagatac
     6241 agtagaaagc agcctgaacc attgtttgcc ttttgcaaat agttcagcat catttacccc
     6301 aaaccagcca acttctttga aaaataaatt tttaaaaaaa gaaaaccaaa accacaatac
     6361 ccaaactcag ttggaccatt aaacctccat cttttactga cattgaacag aaatgggtct
     6421 atgaaagtat ttaatcaaaa ttaatgtcct atccaaatag gggcaaatac tatttggcct
     6481 ctgtgccaag gaggcttgct ggagaggtac tttctccagc cactgggacc gtgggaacct
     6541 tctacttgca gatggcaatt tggtcttgct accattatcg caattcacta aagatctata
     6601 agacaacgct aacagaggac acaagcttta ctttgaaact agaagacagt agccacaacc
     6661 ctacgtggat gtagcaaaat cctattttgt gttccagctg catagcgaac tcatacaaag
     6721 aaccaccctg gctcaggcag acctactaag gattaagaga agggagcaca aaaaacagag
     6781 gtccgtatct gtcccgcaga aaatttggtc tacttctcca taaagctcgc tcctttggca
     6841 cacacaaagc acgccaatcc ttcctacatc cctcccagag tagatacaat tgctttagct
     6901 acgaagagca agagtgccgg tccagctcgg gatcgcagcc actaccgcct gccggacaga
     6961 gcgggaacca acagtcagaa aagatcaaag attctcacaa aacagctggc atgggtttaa
     7021 aattcacgga ctagtaggaa tgcagaaaaa aggtatacat tttgctttca gagagacttc
     7081 tgtaacttcg gctttccatc cgtatggaag gtatattttg gcgcggcact ggttacgcgg
     7141 catgtagaat ttgtaggctt gggtgtgtcg aaccaagagg cttgcacccc taccctcatt
     7201 ctacactgta gagccacgac ttccctccat tttacaccga atttcaaaag cgggcagagc
     7261 agaatcttct ggaacggtgt tagagcgggc tcaggaggtg ggggtcggtg tggaaagaac
     7321 agacgggatt tttttttctc tcttggcgaa tgaggagcgc cccccatcac ccctccttct
     7381 caacatcccc caagcgcgca tgcgcactta ggtggcaaga gggtacttaa ggcacggcca
     7441 ccggggctgc ggcattgcgc ccagcagcgg actccgagac cagtagacct cggcgaaccc
     7501 ttgctctcga ccacccaccc actttcggaa ccatggccgc agtggcagca gcctcggcag
     7561 aactgctcat catcggctgg tacatcttcc gcgtgctgct gcaggtgagt atgtacccgg
     7621 gctttgggtg ggagagggca cccgaaacgc gccccgggac cccttaatcc atatcgcgat
     7681 tgccgcccct acccaaccca tcctatccca attgccgcaa tccctgtgta ccaaagtgcc
     7741 gcggtggtac tatttgtccc cgcccgttcc cgtgcccaca tcctcgcgca gaccctgtcc
     7801 ccagtgcgcc cgcgcccagc cgggaacaaa gactcagggg tgcggcggtc gcccgctgcg
     7861 gacgatcgcg accaacgcgg taagaaatac ccgctatatt caggctcgat cccaggaggg
     7921 aggcggggca cttgtgtgct gtgaaacttt acagttcgca caacagaaaa ttttcacctt
     7981 aaaaaattgc gcaatgcagg aaattttctt ttaaatacac tatgtacaca catataacac
     8041 ttgggtgggt gaaaaagaag ttagaaaatg gcactcctca ggaaaaataa caaaaagaat
     8101 ctcaataagg gaaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
     8161 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8221 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
     8281 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
     8341 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
     8401 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
     8461 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
     8521 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
     8581 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
     8641 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
     8701 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
     8761 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
     8821 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
     8881 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
     8941 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
     9001 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
     9061 accagtaacc tctgcccttt ctcctccatg acaaccaggt cccaggtccc gaaaaccaaa
     9121 gaagaagaac cctaacaaag aggacaagcg gcctcgcaca gccttcactg ctgagcagct
     9181 ccagaggctc aaggctgagt ttcagaccaa caggtacctg acagagcagc ggcgccagag
     9241 tctggcacag gagctcggta cccggaagat ctggactcta gagaattccg cccctctccc
     9301 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     9361 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     9421 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     9481 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     9541 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     9601 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     9661 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     9721 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     9781 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     9841 tcctttgaaa aacacgatga taagcttgcc acaaccatgg aagatcccgt cgttttacaa
     9901 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9961 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    10021 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
    10081 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    10141 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
    10201 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
    10261 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
    10321 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
    10381 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
    10441 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
    10501 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
    10561 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac
    10621 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg
    10681 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt
    10741 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
    10801 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
    10861 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga
    10921 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
    10981 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat
    11041 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa
    11101 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
    11161 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
    11221 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat
    11281 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
    11341 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
    11401 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
    11461 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac
    11521 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat
    11581 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt
    11641 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg
    11701 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
    11761 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg
    11821 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc
    11881 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg
    11941 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg
    12001 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc
    12061 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt
    12121 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat
    12181 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
    12241 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg
    12301 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
    12361 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
    12421 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
    12481 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
    12541 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
    12601 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg
    12661 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
    12721 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat
    12781 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
    12841 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt
    12901 cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag gggggatcta
    12961 agctctagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
    13021 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    13081 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    13141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    13201 tcttatcatg tctggatccg gaataacttc gtatagcata cattatacga agttatgttt
    13261 aaacggcgcg ccccggaatt cgccttctgc aggagcgtac agaacccagg gccctggcac
    13321 ccgtgcagac cctggcccac cccacctggg cgctcagtgc ccaagagatg tccacaccta
    13381 ggatgtcccg cggtgggtgg ggggcccgag agacgggcag gccgggggca ggcctggcca
    13441 tgcggggccg aaccgggcac tgcccagcgt gggcgcgggg gccacggcgc gcgcccccag
    13501 cccccgggcc cagcacccca aggcggccaa cgccaaaact ctccctcctc ctcttcctca
    13561 atctcgctct cgctcttttt ttttttcgca aaaggagggg agagggggta aaaaaatgct
    13621 gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg
    13681 aaagttgcct tttatggctc gagcggccgc ggcggcgccc tataaaaccc agcggcgcga
    13741 cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat
    13801 cctctagagt cgagatccgc cgccaccatg attgaacaag atggattgca cgcaggttct
    13861 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
    13921 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
    13981 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
    14041 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
    14101 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
    14161 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
    14221 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
    14281 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
    14341 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
    14401 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
    14461 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
    14521 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
    14581 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
    14641 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
    14701 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    14761 gcggggatct catgctggag ttcttcgccc accccccgga tctaagctct agataagtaa
    14821 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
    14881 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    14941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    15001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    15061 tccgggggta ccgcgtcgag aagttcctat tccgaagttc ctattctcta gaaagtatag
    15121 gaacttcgtc gagataactt cgtatagcat acattatacg aagttatgtc gagatatcta
    15181 gacccagctt tcttgtacaa agtggttgat atctctatag tcgcagtagg cggcgcattg
    15241 caggaaaaat aagccagaca aaggcactta ctaggagaaa aatggattag ataaaagtgc
    15301 attggaaaag aaacaaagaa acgaaaaaaa aaaaaaaaaa aaccgaattg gaaacacact
    15361 agagaaaaaa atgccccttg caggacaaaa acggaagggg aaagaacatt ttcaaaaaaa
    15421 aaaaaaaaag aaagaaagaa agaaaaagaa aaaagaaaga aagaaagaaa gaaaggggca
    15481 aatcatttta tggaaaaaga gagtgcgcga tatcgcgcgc aaaagtgctt ctcttaaagc
    15541 caaactggaa gaaagcgctt tgtgcaaaaa aaaactatct accccaagag ctccctttgc
    15601 aggaatcctg ctaaaggaat cctttgccaa aggaatcgca tatttccttc aaggtgttcc
    15661 tggaatgctg catttactgg gtaggattcg cttttcgaaa tcctccaggg acacagccca
    15721 ttgcgagaag tgaggtatac ttaagttgtg ggtccaatca gcttgcagcc atgcagctct
    15781 cagcacagtt ggaaaagctc cagctgccct gactggtgga caagctgtgc ctcggccgcc
    15841 actccaggct atgctggaca ggcgggcggg gcagggggcg gggccgggcg ggcaaagcag
    15901 cagcagcaag cagcagtagc agcagtagca cacaggatca tgggtacttc tctaagggtg
    15961 ggtcctgggt ctctcaccgc aggtgttcag gtactccctg cagaagctgg cgcacacggt
    16021 gtcccggacc gggcggcagg tgctggggga gcgcaggcag cgagccccca actgaggccc
    16081 cagctcccag ccctgggcgg ccgtgtcatc aggtgctcct gtgcttctcg accagcatgg
    16141 gagccagtgc cgcgcaggaa tggggggtcc cctgtgttcc ctcgtcagag gagcacttgc
    16201 caaggtcagt gaggggccgg taggccccca gaaaagcagc accgacaatg atgaagatat
    16261 cagttccctt cccagcccct ttgcccctgt cccactaccg gcgggtggga gaggaggggg
    16321 gaagagggga gcaaccctcg agatatgggc gtaggcacca cattctgatc tggaccaagt
    16381 cggaacagta ccatctcagc cgcacaagat cctaccatga agatcgaaca gcccatcaac
    16441 cagcagaatg gacattctga catcaccagc tgaagcccta catctcggtg cagaagagaa
    16501 agtgtcaact gtgtgcagca tggggggagt ggagggcgtg ggtggtggag gaagagggtt
    16561 aagaaaacta gtggggcccc cttgctgccc cttgcctatg gcacacatat tcctgccttg
    16621 ctccctcatt tccccttttc cccccgcctt cgaaagccct ccccaaaatg tgtcacttga
    16681 tttggatata ttcaaccagt aattgaatcc cacctttacc aaaacacgtt ctctaacccc
    16741 cggcccttca ctgatcttgc ttatccctgg tctcacgcag cagttgtggt caatattgtg
    16801 gtagtcgcta attgtactgg tttaagtgtg cattagtagt gtctccccag ctagattgta
    16861 agctcctgga gacagggacc acctccacca aaaataaaaa aatggacctc tcctgtcttg
    16921 tagtgtccta ggaccctgca gggcagtggg ggtgcaccaa aaagttcttt tgtggcttct
    16981 tttgcgactg cgtcacatta tccataaagc tgggcaatgt actcattgtg gaggggcaca
    17041 ccacttggaa tggggagggg gaccacaaga agacaagaat aaactgagat gcccatatct
    17101 gcctcattta aatctgtcct gagataaagg actatttctc atccgcaatg agaagagaga
    17161 tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga
    17221 gctcgccatc agttcaggtg aatttatttg gtggtgccga gataaggtac catggcaggt
    17281 tttctatctg ggaaagatgg agagaaaata actgggactg gccctctaaa tggaatacta
    17341 agggtcatac gggttaattt aagaatataa agctatacca cagatctctc tacccaggag
    17401 ccccaggaaa tgaatgctgg gcaagaagag ggggtggtgg ctgggacaat gagcatgagg
    17461 tgtggcttgg cccctcctcc accaagctta atgctccaga ccccaggatc accctaaggc
    17521 tggccatcct gtcttgattt tccaaccctc cagctggaca ctccctctct tagttttctc
    17581 agttcatccc taagggcctt atcagatcga tagcttgcct tttcccctcc cattgcagca
    17641 gggagggtgg gctggcccct tagttccctt tcttggttag ccagagctgt tcacaagtgt
    17701 cagctgactg cctcaccaaa tggtcctgcc acctgttaag cctcatttct ggctttaccc
    17761 gtccctgtct ctgcacacct gctcctatgc caatgagtta gaaggtcacc agtctctctt
    17821 gctatttcct caaacttcct agtcctggtc atcacattac acactcaggt gcacaaagca
    17881 cctcaaagtt cttgaaccct gacctgcaga tcccccaaga aaccctgatt ttcaaatcaa
    17941 tcactggcac ctccctcttc tgcagggact ttatagtctc attcagcact agggaattac
    18001 gggggaaagt agcacagatt ttggtgtggc cttggctttt ccacccagcc aggttgcttt
    18061 tagttcccta ctaaggtcca gatgagcccc atacacatca gagaggcggg attccacagg
    18121 acttggatgt gaaacccatt ccaaggcgcg gtcttcctta gcagggtaga tacagctgct
    18181 gctaaggcct gaagtcaggt caggtcctgt gcctgccccg tagttactca tctgcagtca
    18241 gtggctacct cctttatggt tagaaacttc tgtggtttgc catactaaac tcattgaacc
    18301 ttgagtacat atacaacaca ggttccaaag gaaggttgac tctccccttg gagatctgga
    18361 gtggggggcc tgatgtcact ctattgttcc atgttctaac gtcagcccaa attagatgcc
    18421 agctgagtac acaaaagcca aaccagggag aaaggagtct ggctactctg gtcatagcat
    18481 atcctgtgcc catccctaag ccaggaggct actactggta actccagggc tttccagatg
    18541 atcttggtaa aacctttttc ttccatctat tatccagcag tgatgagtgt aggtggagtc
    18601 ttcaaggcgc cctggtgacc ctgagaatgt gaatgtctac tacccacttt cttctcccac
    18661 tccacaccct tttgtcctga cagcaaacta aaccctccag aggaggcagg aatgtgttcg
    18721 tgccctttct ttgtgtcaaa cctgcaagcc tgatacaaaa ccaccttgtt tatacaatgc
    18781 ttccacaagc tatgcattaa aacattttat tctcacataa agcaatggga tgtgtcttta
    18841 ttgcaaagtc cttagtacac agaactaaat atcccttttt cttaaagagg ttgctacctc
    18901 caaagaggag caaaggtgga gaaggtgaag tggaacagct ttatgggaat ctagcaacac
    18961 acgagattcc aggcactaat gccagagaag gcctgaccag ctcaagctca gtagggtgga
    19021 ggttactaag taccaaaact tttagagatg tgggttctag gagactttta agtctatctg
    19081 taaagtaact gaattgtgac tgaaatgtat ctgagggaca ggacaggaat taagattttt
    19141 ggctttgact ctggggctgg ccaaagacag cttaattaca cagaggtttt gaaggaagca
    19201 ggctcgctgc tgactcctta ctagatatct actttcaaac ttggatcgct tcaggagcca
    19261 acatcccaaa gggcgagcct ctgagctggc agctaatctt ccccaggaga tttaaatgtg
    19321 tatacacaaa cataagacta atttaccaaa gtctcttttt tcaggtgacc actgacttct
    19381 tccatatgcc tgagagacaa tgtttgccac aatcttctct ggaaataatc ctgtccccct
    19441 ccctccacag ggctcatacc acaccaggat acagattcct gaacattccc agggcagagc
    19501 cagaagacag agaggaaggc aatacagggc tcttggtaaa tcacacagtc acaagcttga
    19561 atggaatgcg tgtatttcaa agagaacaag taaaaagggc agcctaactc tcatccccct
    19621 agaaataaca ggtaagaagt taaagtgttc tgtcaacaac tttaagatga ccattttcag
    19681 atcgagaatt gatctggttc ctggaaactg accaaggttc cagaaatcca atctttaaac
    19741 aggaactctg gccattaatg aatcttgact taaaagaaac aagccagctc aatctaatgc
    19801 ttgccagggg cacataaaat ggaacttttt ttgggtcctg gctttggtgt tgcagttcag
    19861 atggctgaca ggatggctgg accatgtcag acagggcaga gctgtcccca atatatcagt
    19921 tccctgaaag ggacaaagaa gatccgaacc tataaacaga aataacctat aaactagaac
    19981 gggccccagt ttcccaagta aaaagagaca agagacttag aagaaaccta aaggtggttt
    20041 ggcagagaaa ggaaaaaaaa aaaaaaatcg actgtcccca tcccccattc ttcctttcca
    20101 gccaggcact ccgttccttc tgctgccctt gagtgccact ccccacaaac tggagaggga
    20161 accggtgtcc agactcgacg aaaagttggt ggaagtgaga agcacacggg acccgtagca
    20221 gatggtcacc ctatcactcc ggacacaaaa gagagcgttc ggggcccact tctactttct
    20281 ccccacactc cttcggataa gggtggggga agaaaaagaa aaaaaaaggg gggggggaga
    20341 gaaactgaag tggcgaggaa agagtgtcca gcctcccaga agctgagccc ctcgccaagg
    20401 tccggaacag aggaggtggt ggtcgcgccc gcccctccca gccccgtccc gggagcgcgc
    20461 tgaccttgga ggccagagtg acagctaagg aaccccggaa cccacccaag ggacgggaag
    20521 ggaccacccc caaggcccag gttgggacga aaaggggagg tcatctgacg ccagacaagg
    20581 gtgcgcccga ccccggccgc gcggaaagcc ccggcgagag gccaagcgac gcctaaggtg
    20641 accccggagg gctaacatgg cggcgcggcc gcaccgagcg ctctcaccct gcccaggcgg
    20701 gcgacgccac gatacctgcg agccccacca tggcactgcc gccactgtcg ccgtcgccgc
    20761 cgctgcttgc ctccacctta gctcgccacc aacgccgcag gagctgggga gacgccccct
    20821 tcgtcttcta gtggagcggt ccaaggaccc ccggtccaga accgagctgt cttctgagaa
    20881 cgcctgacaa actggctcaa agcgaggcta ctctcagaca ctctcccctc agtatccgcc
    20941 acgaaatgtc ctttctgagt ttttagtaga cttgaataaa attatggggc gctaaggtct
    21001 tcaaacccgt ggaatatttg gtcaggcgta tcttaggcag ccccgaacct ccggcccatg
    21061 gtccccttca tgcatatgca gcagattgtg attcttactc tttgagagct ccgcgggcga
    21121 gcgaggtgta gttacgctga tgagcgtaac tccgcagaat ccttagcacc ttgtgatttg
    21181 tgtactttgg gaaaacttct cgtggctcag atctggccct tcaaccacgg gctgaggacg
    21241 tgcttcctcc ttggtgagga aatggcttgc cccaggcttg ttaatgtcta gatgagtcag
    21301 tgtggtcact tactagctta ttagccacgt tcctgacctc atactgtgcg caagcgcagt
    21361 gctagaggct aaggaggtac aattctactc ctgaccccaa ttttctcagt caccagggtg
    21421 agagtgagtg tcgggggata gtggagagac taccacccag taaccacttg tagtcgcacc
    21481 agtacatgat ttgtaattag gcatcgtgga aggcactagg aggcaaagta aagagaggag
    21541 aaatgagtgg aatcctggtg tctggagtgg gcgagggttg gggccgtctt aggcatattg
    21601 agtttgtgca ttgtgtgagt gaggggtctt gttccagaca tggaggtgac aaccctaagg
    21661 gtggaaggtg aacaaggaat atgacaggta ccttggtcat gaatttgaat ttttgtcttg
    21721 agagacatgg gaagctatgt tcttcgaggc aatgaaaaga aatctgggtc tctccgaggc
    21781 cattgacttt cagggttaga aaacatgaaa gtgaggtttg ggacaggaag ccagttaagc
    21841 aac
//