LOCUS       KO-first_condition_ready_46932_MGI:104669        21530 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Man2a1 targeting project(s): 46932
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 185494
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     misc_feature    4112..9728
                     /note="5 arm"
     misc_feature    9756..9780
                     /note="B1 Gateway"
     misc_feature    9793..9840
                     /note="Frt"
     intron          9841..10715
                     /note="mouse En2 intron"
     misc_feature    10713..10715
                     /note="Splice Acceptor"
     exon            10716..10888
                     /note="mouse En2 exon"
     misc_feature    10900..11493
                     /note="ECMV IRES"
     gene            11494..14554
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14593..14836
                     /note="SV40 polyadenylation site"
     misc_feature    14840..14873
                     /note="loxP"
     promoter        14900..15419
                     /note="human beta actin promoter"
     gene            15445..16239
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16439..16680
                     /note="SV40 polyadenylation site"
     misc_feature    16697..16744
                     /note="Frt"
     misc_feature    16751..16784
                     /note="loxP"
     misc_feature    10730..10807
                     /note="FCHK std_gpr"
     misc_feature    16800..16820
                     /note="B2 Gateway"
     misc_feature    9729..16850
                     /note="Synthetic Cassette"
     exon            17254..17381
                     /db_xref="ENSEMBL:ENSMUSE00000493264"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000493264"
     misc_feature    16851..17593
                     /note="Critical Region"
     misc_feature    17617..17650
                     /note="Downstream LoxP"
     misc_feature    17594..17673
                     /note="synthetic loxP region"
     misc_feature    17674..21530
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg aactttgctt tatcagcaca gttttctccc
     4141 cttgttatgt ctatgctcta aggtgcttta gtgacgctca aaggtcacaa agtgcaccca
     4201 gtctttgacg tcgatgtctt gttcagctct caagtgtctg cctttgtctc tggctgcctc
     4261 ccaccttaca tttcaccctt atgtatgcct ccttaatgcc ctgacttctg gcataccgac
     4321 ctgctgctgt cttcatacgc gttattgttt tatgcattga gtaatttcat acgtaatctt
     4381 tcccatgact cagggaaggt attatcttac ctcagaagcc ttcccctact gactatgttc
     4441 ttcattggac aaatatttac tgtgctaata tccagggtaa gctctcagtg aacaaagagg
     4501 gcacagttaa gcacaccatt ataaaagcat gttgttttca gtgaggtttg gaagcctgca
     4561 gtattcagga tgtgactaaa accccagtca aggagaagat gtgagaggat ggggaagaca
     4621 ctggaggttc tgaggagaaa actgtgatgg gcgagaactt ggcacctcag acactggaag
     4681 agattggcta ggtgcagcct caccaaagag gatataagat acccttccgg gctgttaatg
     4741 ttgatagcag caaaggttgg ggagaaggtg caacaatgtg aacatggtag tggcttgctg
     4801 ttggaactta aaggggtgac ttccaaagac ttccaaatga ccatatgtgt tgtgtgtgag
     4861 gagctgatct agaaatggac ctgagagaga gtcttttcat attcacagca gacttgaaat
     4921 ggttcaggtg tctgcaatca agatggtaga ctaacacacc aatagaatcc tatttggcaa
     4981 gaaaaggtga attagtgaaa cacacttccg tttggctttt ccagacaggg tttctctggc
     5041 tagtgctggc tttcctagag ctctatttgt aggccaggcc tcgaattccg agatcagcct
     5101 gcctctgact cctgagtggg gagagtaaag gcatatgcca ccactgcctg gccttgaagt
     5161 acatttcttg aggaactttt agaaagtttt taatgaaagg gacttagctt agtcactgcc
     5221 agctggtgac cctgcagttt attccttgtc ccttgctaat gcagggtgta tcatttgtct
     5281 gtggtctgac ttttctgagt gactttgggt gtctatgcag actacagttg gatagatcgt
     5341 gggggcagcc tgggctaata cctttttcag acatctggct tctcagactg ttgtacaacc
     5401 ttccgatctt tggaagctga tgggtgagat gtggaatgtc actctccatt cagccaccca
     5461 aatgaggtta cattttgttt tttagagatc agttatttct gaaatctcgg acacttactc
     5521 tttgtttctc ttgtgtgtta ttggtgcccc tttcttacct tgctatgctg ttaggggctc
     5581 ctacatggta aatgatcaac agggagttca tgcccttccc gagcttgtga tgttttggat
     5641 ggaaacgaca gtggagatgc aggtgtataa ttagtgtcag ttatgaatgc tatgaaagag
     5701 agttttaggt tttatgaaag gtgatagctt tgttggggtt tgcataggat gtgacggttg
     5761 gtagtgcgtt cctaagagcc agtgttagac cagtgttaga agggcggatg ctttaggttg
     5821 agaagatact gacaacaggt caaggggagg ggacacaccc aaatgaaatg aggaaggagt
     5881 aatgcagacg actcagttgt ttttcttata ctccatcttt gaaggctggc attcagaata
     5941 ttagagtata caaggatccc agattcacat atggaagggt ttaaaacaag tcttccttct
     6001 gctttcccac tggatactgc acacagaagg cttgtacatc aggtgtctgg gcatgaattg
     6061 attctgtggg agacaccatg taactaattt tgcatttaag aatggaaccc agtgtgtcat
     6121 ctcctatcat ttcagatttc tcacaatgca cgattacaaa ttcatctcgc tttctcatgt
     6181 gagattaatt tgtgactctg tgaagcttgc tagatgatgc attgtttgct accaagttgt
     6241 ctgtaacaat ttccccatta gggagaaaag ctctttgagt atatccttct gtgtgtgacc
     6301 aaattccaaa tgtctgatga ctcttcgtgg aggaagacat gacactctct aaaaatgatt
     6361 tacagcttct tgagcactgc agtcaatcct gaagtgtaaa atctgtgtct tgggcaaaat
     6421 gagcagtgac agattcgctc agcttatctc tgatcagtta cttctctgag cgctttatac
     6481 ccaatccccc agcactgcag tagagacgtg gttaacgtcc tcaaccaagc agctagccac
     6541 cattgtagaa agtgatgtgt gagccttggc ggttttaaac ttagcagctc tcaagtgggc
     6601 tcttgaattc actccatgcg tatctctctt cccctgtttg catgtagctc tcgtgtctat
     6661 tacttaagat catgatgtgt atgttgaaaa gtttcccagg aagctgagag ccttccacca
     6721 gtagagagac ttaagaaacc agctacacag gacagacctg cattgtttgc ttgtccctct
     6781 gcttctcaca gaaacgcttc atccgatgct gctgaaagcc aagtacacag aagtgccagg
     6841 atttaagtga acacttctgt agggacaggt gaatcccacc ctggctcttt tttattatgg
     6901 aagtctagag agttcagtta cagccttatt cattttcatg tgacggcaaa caacagggat
     6961 tttaagtatg acacctcttg tactctcaca cagttttttt tttttttttg cgtgcaacct
     7021 ttttgttctt gttgaccatg gtgagtgggt gaatggtcac agggcaggtt tttctgtcac
     7081 cgtcagaaag cacttctgtc aagctgaacc ttcctgagac atttggcttt ataatctgtt
     7141 ttgggggatt tttttttgcg tgtttttgtt tgtttgtttg ttttgttttg ttttcctttt
     7201 ctaagggtca catggcaggt aaacagacac cgccatggaa taggtaaact cactaacctg
     7261 gtcatttctg tccctgatgt agtacatagg ctgtggctgc atctttaaga ttcaccacca
     7321 atcaaagaaa acacatggtg ggccttgtgg ctctagctgc atatgtaaca gaggatggcc
     7381 tagtcgggca tcaatgggag gagaggccct aggtcctgtg aaggttctat gccccgtata
     7441 ggggaatgcc agggccagga atgggaatag gtgggttggg gagcaggggg agggggcagg
     7501 ggttagggga ttttcagagt ggaaactagg aaaggggata acgaaatgta aataaagaaa
     7561 atatctagta gaatatatat aaatatgtaa atatttatat atataaagat tcacactgtt
     7621 ttcttccact tgatattctg ccttgtcgga ttggataaac gatgctaaga taactacagt
     7681 tgttcctttt atgtcatgct tttgatctgg tttcggcctg ctcatgcaga ttttcttaag
     7741 aacctactgt tttgtggggg agaaaagcac acattttata aaagtccctt ggtttctatg
     7801 ccatgatctt ggctttttca tgtgatttta aaaattctct ctgatgccta tatgaatgcc
     7861 agtatttgaa caacccactt ttcagcttta gagttggatt cgcttttgtg tcctgaatta
     7921 gttactctct tccagatgtt tctttcggat gtacctaaaa cttctcttca gtcgcactcc
     7981 tctcttccta cctgacctgt agctgtcctt aagacttcct ttttaaaggc accatgccct
     8041 tccagcctgt gagacggagg tactcctcat ctgacttccc gttgctctcc atcccccctg
     8101 gaagcccagc tgtccgtcac tggctagccc agactcatgc tcattactgc aaagcgactt
     8161 ctcccacctc tctcgcggta ttcattaata tctctctatt aaacccagac ttgggaactc
     8221 caagccacct ctgtgctatc acagtggtca cctcctcttg ctctagtcct actcagattt
     8281 cccagagata ggtttttatc aactccctct gctcctcttg catgttttct accactgtca
     8341 acataattgg aactccattc taaatgacct gatcctgact gatttatgtt atatgtgagc
     8401 ctgacctaat ttaatctact cgttaattac taatgttaaa cggacagtct cgtgcatcta
     8461 tttttcaaag cagggtactc agtgccaggc atgtgatggc acatgctaat ccctgctctc
     8521 ctggtgcttg ccttctgttg gtgggtagga gggagaggca gataggaaca aacaggcaat
     8581 tttagtttgt gataaaggct atgttgttgt atatggagga ggtatggaga agtgcttaat
     8641 tgcctgggga ctcatggaaa cttctggatc acttatattt gagtggagac actaaggcag
     8701 ctggaaccat ccaggttgat gaagaggagc aacggtgaac ttgcttggta tcatgtagta
     8761 agaaagaggc atagtcatta gggtaaccat ggcgtccaga tgagagccca gtatttatca
     8821 gaggagcatc catggcttcc caaggaaggg gattggctaa taacagctgc agctggagaa
     8881 actcttgctg tacatactat ttagggcttc tcttgctgga ctcttcttgg agagcattca
     8941 ggtctttagg ataggagggt ttttgtttgt ttgttctttt tttttcagtt tttcattgtc
     9001 aaacaaaact tgacatgttg aaaaggggaa aaattgtctt tggacttatt attattttct
     9061 tccatttcac tctagttctt gttaacttgt aggttctgtc tagtcaagcc caggaaataa
     9121 tttgtcttta ggcaattata tctgaacatt tccaaaatac atgttgatta ggatagtcag
     9181 ataatgaaat caatagcatg tccatgtttg atgggcaggg gttatactct gcatgccagc
     9241 ccatgcttac atgtaggtac caacctctcc ttttgaggac aacggtcaga tccgtgtggc
     9301 ctctggaatg tggcaaagat agacctaaaa gtcagcacag agcaaatagc tttaaaaagc
     9361 cttttgtcca tgagagagga gggctgtatt gtcatggacg tagttggctt cttaaataaa
     9421 tgcaagtttt agaaatccca gctccttcag gtcaggtcat gtcttgatga aagcctcctg
     9481 actctcggaa cttccacacc tcacaacctg acagtctttg atattacaga tacaggctaa
     9541 ggctcttcta cttcttcaga tactttcaac ttactggtag ttcataattt tcttttcttc
     9601 ttagagaaag ttatgcgaat aattaacaat tagtactcat tgtcttgtat gatattattg
     9661 caaaggaatg ctcaacagac aaataacagt aatgacacag ctttttcaac ccatcataaa
     9721 aataggagaa ggcgcataac gataccacga tatcaacaag tttgtacaaa aaagcaggct
     9781 ggcgccggaa ccgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     9841 gaaccctttc ccacaccacc ctccacactt gccccaaaca ctgccaacta tgtaggagga
     9901 aggggttggg actaacagaa gaacccgttg tggggaagct gttgggaggg tcactttatg
     9961 ttcttgccca aggtcagttg ggtggcctgc ttctgatgag gtggtcccaa ggtctggggt
    10021 agaaggtgag agggacaggc caccaaggtc agcccccccc ccctatccca taggagccag
    10081 gtccctctcc tggacaggaa gactgaaggg gagatgccag agactcagtg aagcctgggg
    10141 taccctattg gagtccttca aggaaacaaa cttggcctca ccaggcctca gccttggctc
    10201 ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt gtgggttaca taggaagggg
    10261 gacgggattc cccttgactg gctagcctac tcttttcttc agtcttctcc atctcctctc
    10321 acctgtctct cgaccctttc cctaggatag acttggaaaa agataagggg agaaaacaaa
    10381 tgcaaacgag gccagaaaga ttttggctgg gcattccttc cgctagcttt tattgggatc
    10441 ccctagtttg tgataggcct tttagctaca tctgccaatc catctcattt tcacacacac
    10501 acacaccact ttccttctgg tcagtgggca catgtccagc ctcaagttta tatcaccacc
    10561 cccaatgccc aacacttgta tggccttggg cgggtcatcc ccccccccac ccccagtatc
    10621 tgcaacctca agctagcttg ggtgcgttgg ttgtggataa gtagctagac tccagcaacc
    10681 agtaacctct gccctttctc ctccatgaca accaggtccc aggtcccgaa aaccaaagaa
    10741 gaagaaccct aacaaagagg acaagcggcc tcgcacagcc ttcactgctg agcagctcca
    10801 gaggctcaag gctgagtttc agaccaacag gtacctgaca gagcagcggc gccagagtct
    10861 ggcacaggag ctcggtaccc ggaagatctg gactctagag aattccgccc ctctccctcc
    10921 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
    10981 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
    11041 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
    11101 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
    11161 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
    11221 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
    11281 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
    11341 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
    11401 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
    11461 tttgaaaaac acgatgataa gcttgccaca accatggaag atcccgtcgt tttacaacgt
    11521 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    11581 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    11641 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
    11701 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
    11761 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
    11821 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
    11881 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
    11941 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
    12001 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac
    12061 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
    12121 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
    12181 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
    12241 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
    12301 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
    12361 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
    12421 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
    12481 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
    12541 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
    12601 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
    12661 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
    12721 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
    12781 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
    12841 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
    12901 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
    12961 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
    13021 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
    13081 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
    13141 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
    13201 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
    13261 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
    13321 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
    13381 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
    13441 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
    13501 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
    13561 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
    13621 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
    13681 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
    13741 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
    13801 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
    13861 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
    13921 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
    13981 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
    14041 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
    14101 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
    14161 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
    14221 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
    14281 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
    14341 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
    14401 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
    14461 ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc
    14521 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcagggg ggatctaagc
    14581 tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta
    14641 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    14701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    14761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    14821 tatcatgtct ggatccggaa taacttcgta tagcatacat tatacgaagt tatgtttaaa
    14881 cggcgcgccc cggaattcgc cttctgcagg agcgtacaga acccagggcc ctggcacccg
    14941 tgcagaccct ggcccacccc acctgggcgc tcagtgccca agagatgtcc acacctagga
    15001 tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc gggggcaggc ctggccatgc
    15061 ggggccgaac cgggcactgc ccagcgtggg cgcgggggcc acggcgcgcg cccccagccc
    15121 ccgggcccag caccccaagg cggccaacgc caaaactctc cctcctcctc ttcctcaatc
    15181 tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga gggggtaaaa aaatgctgca
    15241 ctgtgcggcg aagccggtga gtgagcggcg cggggccaat cagcgtgcgc cgttccgaaa
    15301 gttgcctttt atggctcgag cggccgcggc ggcgccctat aaaacccagc ggcgcgacgc
    15361 gccaccaccg ccgagaccgc gtccgccccg cgagcacaga gcctcgcctt tgccgatcct
    15421 ctagagtcga gatccgccgc caccatgatt gaacaagatg gattgcacgc aggttctccg
    15481 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
    15541 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
    15601 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
    15661 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    15721 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    15781 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    15841 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    15901 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    15961 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
    16021 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    16081 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    16141 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    16201 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
    16261 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
    16321 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    16381 gggatctcat gctggagttc ttcgcccacc ccccggatct aagctctaga taagtaatga
    16441 tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc
    16501 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    16561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    16621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
    16681 gggggtaccg cgtcgagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
    16741 cttcgtcgag ataacttcgt atagcataca ttatacgaag ttatgtcgag atatctagac
    16801 ccagctttct tgtacaaagt ggttgatatc tctatagtcg cagtaggcgg aactgtgttc
    16861 cagggacaga ggtaaatgcc gctttttata tggtatattg gaacagacgc catccgtgaa
    16921 atggcagctc tccagccaac accaagcaca tggcctgaat acagtgctct gtgagattca
    16981 gaaataaacc acacgactcc tgtcatgagg agtctgtgct gtgcctgtgt ggaagaagtg
    17041 tcctaggaaa agaagggtca cccaagattc tttaatatga gcacttcatc ccaggggcct
    17101 gcagttgaaa ttcaatgact tattaaaaaa aaaacaaaat tccccctccc tcatgtaact
    17161 actgtccatt tgtaaagatt gatcttagac gtttatgtat tgtattttct tctgtgagct
    17221 ctgttatctt aactctattt gtttcctccc tagtttacta cagaatggtc agctggaaat
    17281 tgtgaccggt ggctgggtta tgcctgatga agccactcca cattattttg ccttaattga
    17341 ccaactaatt gaagggcacc aatggctgga aaaaaatcta ggtatgtctt tatatctgtt
    17401 cctcctctcc cccttcattt gggctgtctt gggaagggac actcagggga tctgaactaa
    17461 ttttcctaat aattgttgaa taatttatcc ttcattaatt atatttatta ggcctcaatt
    17521 atatagacat gtgatagttt cattgaatat tatccaattt ttctttatat attacttatt
    17581 gactttcatt tgtgagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat
    17641 acgaagttat ggtctgagct cgccatcagt tcaagacttg ttgctaagag tcaagaccct
    17701 attattagct tcattcaaat tgacggtggg acagaaaaca atagaaggca tttatgtgtt
    17761 gtatcatgga gaatcctgga cctagaggag ttatgggagc tccaggtatg gttccttcca
    17821 ggggacgtaa cttacacttt agagattgtt tccttggtct caccttgttt gtgtgttggt
    17881 ctgtaccctg ctttttctag gtagcttata agaaagatga ccctaggccg tagaaatact
    17941 accccccacc cccgcctcca ggatgagttt tgttttgttt ttttgttttg tgttttaata
    18001 ctttatctca ttggcccaca agccaggtgc ttaattctga gatgcaggac tggtttcaat
    18061 gacattgctc tcctcgggaa gtgactgcat cacacctacc tgtgatggcg gggcctctct
    18121 ctgtgtcact ggacccatgg gtaggatgct ggctgacagc atcagacatc acgtacaatt
    18181 caccaaaacc gagcaaccac tggcatcttc tctatgcctg ccactcgaca ggaagccctt
    18241 tgcaaatatt tcatacctca gtataacttt tatgcttttc tttatgatca gcatctgtga
    18301 tttttattga agttatttgt gggtaatttt attttgtctc cattaggaat caaagtctct
    18361 cattattaca attatttctt tcctttttat tttaacacaa ttttaaatct actgagaatt
    18421 aatcccagaa agcttccaca catgcctgac atggcacatt cacgtcaatc tttgttccct
    18481 tcccacccca ctgtggtggt ggtggttgtt ttggaaacct ggagaattca ctgcagatac
    18541 tgcatctgtg gaaatgcact gctgcatctg ggctgagatt ctaacctcaa aagccttact
    18601 tgtgagtctg tccttggctg ctcctggact cttggattct ttacattcaa gtgatctact
    18661 gcttttaaaa gcatattgtg tgtttaacat ttgcttcccc caggaacctg accccttagt
    18721 gtgtgtgtgt gcttgtgtga gcagtgccct gtggaagttc caggcactga atgtgtgctg
    18781 agtttcttag ttgatggtgt gtcacacagc ttcttcctat tggttgctag aggaattaca
    18841 tgtgcatgtg gggcccacac tttggaacat gacctcctct ggatgtggtc catgttcctt
    18901 ttatatttgg tgcccttact ctatcctaag tcatagctgt gtgatagtgt tctgaagttc
    18961 ctaagtgtgt atgtgtgtgt gtggacaggg gtgggggtgg gggttgggat gtaaaaacag
    19021 gatccatttt tttgctgcat acaagaaaca cacctcaaca tcaaggatag acattacgtc
    19081 agggtaaagg attggatgaa gataattcaa gaaaacagac ctaagaagca agctggtata
    19141 gccattttaa tatctaacaa tagacttcaa accaaagtta atcaaaagag acagggaagg
    19201 acagttcata ctaatcaaag aagtgtccat tgagatgaca tttcaattct tttttttcat
    19261 gtgttatatg cacatattct atattttaaa cttttcattg attctttgtg agttgcacat
    19321 catgtacccc agtcccattc atctcccatc cccttatatc tacattttat cattgcaaac
    19381 tcctcactaa aatgcaacac gcacaccggc acacacacac acacacacac accaccacca
    19441 ccaccaccac caccaccaac aacaacaaca acaacaacaa caaagaccga tgtatagaaa
    19501 atatctcatc atggaagctg tagtgtgtca cagtgtgtcc caccgtgtat cctccttcca
    19561 cacatcttca cttgcaaata ttcattgcaa gagtcattgg tctggttgtt tatcattggt
    19621 ctgtgagacc tctggcttct gtgacagcat cagtattgaa tcctcactgg aactcctcct
    19681 agttatcctg ttgttgccct gtgtcatgga gatcctgcag ctttggatct gcaggacttg
    19741 cccccttcat gtgattttgg ggtaggccag ttccaagcct tcagtctgag cctgggtggg
    19801 agctgctttg gctaggtcac ccaatgttgc catcatcagg aggcagggtc agctcttggg
    19861 gccagctcca ctgtgcaggg ccgctctccc aagtgctaca gccagagagg ggccgggcca
    19921 gctctcctgc tctcaggact ccagggccag ttttcctgag tgctgcaggt ggtgaggcac
    19981 agggacccta ggcccagttc tcctgactgc cacaggtggt gagggatgtt gggaggggat
    20041 agcacaggct cataagctct aacaactcag caagccaagg gtgatagtgt gggactaggg
    20101 ggaaacttac tgacctactc aggacatccc ctttccttaa atattgaaat atgtcctaag
    20161 ctaagagatt cactgtatac aaacatgact ttcagatttt tcttaatgtt taacttttgt
    20221 tgagatatag cttacacatg gttcaatgga taaatcttga gagactggtg agttttgaca
    20281 gatatacatg ctcatttcat ccctttctct ccaccttcca gccaaatgct ccctgttact
    20341 atgactgtag aggatttctg ctttgtgaag aactcacata tggactcatc acatactcct
    20401 ttgcatttag tttctttgtt cagtatactt ttgtcttgat tgaggcagat tggtgtataa
    20461 taaagtgcgt ttacctatag tctgatgggt ttctgtccca ggctccgttt ccctttttgt
    20521 aagtaagccc tgccttcatg gctctgggga ttggttctag aattccccac agataccaaa
    20581 tgataccaaa tgcaaggtgc cttcaacaaa atgatgtagt gtgtacataa aactagatgt
    20641 ggctcagttc aaggccctga gttctatttc cagcatcata taaactgttc atggtggtgc
    20701 gcacctgtag tcccagcact tgggaggtgg aggcagggga tttgggagtt tgaggtcatc
    20761 cttgtctacc tagtagattc caggtcagct ggagatacac aaaaccttgt tggctcataa
    20821 ctcttctaac tccagttcta cagggtctga tgccctcttc ccggctccta ggacacttca
    20881 tgcagcctgt ctgtgcacat ccttttacag gcaggcaaaa cactcatata tacaaaatga
    20941 atgaatgaat gaatgaatgc agggggaata tagggaatcc attggctaag caagaaacag
    21001 tcgcaagtta accaggcaaa acctggtgca caaccataag tcatgtttgc atttgtgtag
    21061 accctcagtt cccctcagcg aggttttcac cgcttttatt gtgaaagctt tgcttctttg
    21121 ttgaatttgg ttctagattc tttattttgt tagctgccat tgtaagtgag atttactttc
    21181 ttgatttcag ttaggactca ttactggtat tgtgtatagc tacgtatatt gaattggttt
    21241 ttggtttttt ttgtatagag atcttatatc tttctttttg tgataggttc aggtatatgt
    21301 tgctaactca cttctgttca tggctgagta gtgtttgatt tttaaactag attcctgtca
    21361 gctgtccgta gggtacttct gggtatgagc tgcatgacac aggcgttcat ggcagttacc
    21421 tgccagctct ttctaggcat gtgtttcatc gtcactgata agtcctggga atagacttgc
    21481 tgtagggctg aggaagcttt tcaaagtgat tttactattg tacgttcaac
//