LOCUS       438229#L1L2_Bact_P#L3L4_pD223_DTA_T_spec        22036 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 438229
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000058548
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6188..6730
                     /db_xref="ENSEMBL:ENSMUSE00000898710"
                     /rank="1"
                     /note="ENSMUSE00000898710"
     exon            7064..7079
                     /db_xref="ENSEMBL:ENSMUSE00001208964"
                     /rank="2"
                     /note="ENSMUSE00001208964"
     misc_feature    4275..9211
                     /note="5 arm"
     misc_feature    9239..9263
                     /note="B1 Gateway"
     misc_feature    9276..9323
                     /note="Frt"
     intron          9324..10198
                     /note="mouse En2 intron"
     misc_feature    10196..10198
                     /note="Splice Acceptor"
     exon            10199..10371
                     /note="mouse En2 exon"
     misc_feature    10383..10976
                     /note="ECMV IRES"
     gene            10977..14037
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14076..14319
                     /note="SV40 polyadenylation site"
     misc_feature    14323..14356
                     /note="loxP"
     promoter        14383..14902
                     /note="human beta actin promoter"
     gene            14928..15722
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15922..16163
                     /note="SV40 polyadenylation site"
     misc_feature    16180..16227
                     /note="Frt"
     misc_feature    16234..16267
                     /note="loxP"
     misc_feature    10213..10290
                     /note="FCHK std_gpr"
     misc_feature    16283..16303
                     /note="B2 Gateway"
     misc_feature    9212..16333
                     /note="Synthetic Cassette"
     exon            17092..17189
                     /db_xref="ENSEMBL:ENSMUSE00001226692"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001226692"
     exon            17407..17566
                     /db_xref="ENSEMBL:ENSMUSE00001254499"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001254499"
     exon            17641..17780
                     /db_xref="ENSEMBL:ENSMUSE00001304550"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 3 ENSMUSE00001304550"
     misc_feature    16334..17916
                     /note="Critical Region"
     misc_feature    17940..17973
                     /note="Downstream LoxP"
     misc_feature    17917..17996
                     /note="synthetic loxP region"
     exon            18816..18884
                     /db_xref="ENSEMBL:ENSMUSE00001295970"
                     /rank="6"
                     /note="ENSMUSE00001295970"
     exon            19624..19690
                     /db_xref="ENSEMBL:ENSMUSE00000652389"
                     /rank="7"
                     /note="ENSMUSE00000652389"
     exon            20578..20753
                     /db_xref="ENSEMBL:ENSMUSE00000652388"
                     /rank="8"
                     /note="ENSMUSE00000652388"
     exon            20843..20890
                     /db_xref="ENSEMBL:ENSMUSE00000652387"
                     /rank="9"
                     /note="ENSMUSE00000652387"
     exon            20972..21017
                     /db_xref="ENSEMBL:ENSMUSE00000652383"
                     /rank="10"
                     /note="ENSMUSE00000652383"
     exon            21116..21270
                     /db_xref="ENSEMBL:ENSMUSE00000652379"
                     /rank="11"
                     /note="ENSMUSE00000652379"
     misc_feature    17997..22036
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattaatt aaaacctctg ctagtcatta cccccccaca cacgcacctt
     4321 tttggtctat tttgctctct gtagctgaga atgaccttgt actttacctc cctaaagaac
     4381 aaaccaagga ctccagtgca tgctagccaa gcaatctgcc agctgaagca tgtgctgcag
     4441 tccctagtaa atgcaatttt aaaaattata tttatctgtc tgcatgtcat ggcatgtgtg
     4501 tagcggtcag aggacagctc acaggagttg gttctcttct accatgtgag tcccggggac
     4561 ccagctcagg ttaagacacc gccatcttat ctttctgtgc cttctcattt tgctaccctc
     4621 ccaccagctt tacattttaa tgcatgcaaa gctagaggag acttttaggg taacaggtag
     4681 attggagact gcagataaga gccaatagat ttaggattgc caaaagaagg ccttggctat
     4741 gctactgaag ggtagcccag actgtgtctc agagacacga tgttggagct gggaggaagc
     4801 tttgctggga gagtgctggt ccagcatgaa atcctggatt cagtctgtag cactgaagaa
     4861 ctggacacag gggttcatgc caggaatctc agcacttggg gagtggaggt aggcggacct
     4921 ggcaggcaag gatatctttg ggctacatag agaatttgag gccaacctag gacatatgaa
     4981 acactgtcat gataataaca atcatgggag tggtgagaag gttcggtgtg taaagtgttt
     5041 gctgcataag catgaagacc tgagttccga ttcacagcac ccacaatgcc gggccctggc
     5101 agaatgtagc tgtagctcca gcactgagta gagaagacag gtgcgtccca ggagcttgct
     5161 agtccaccag cctcgctgca accgcaagcg aagagccagg ttcagtgaca ggtcctgtct
     5221 caaaacaagt cggacaccaa gcagggacca attcatttca tgaatgcaaa agcaagagga
     5281 gactttcaga gtaataggta aattggaggc tgcagatatg agccagtaga tttaggactg
     5341 cctctggtgt cagcctctgg cctccgcatg aacctgcatg gacaagcaca cctgcccacc
     5401 taaatgccca ccatgacaca cacgcacacc taagttttta aaaaaattta attttaaaac
     5461 agagacaagg aggacaatgg tggtgtgtgc ctttagaccc aacaccgcac ttgggaggca
     5521 aagatggatt tctaagttca aggccagcct ggtctataga gtgagttcca ggacagccag
     5581 aactacacag agaaaccctg tcctgagggt gtgtgtgtgt ttgtgtgtgt gtgtgtgtgt
     5641 gtgtgtgtgt gtgagagaga gagagagaga gagagagaga gagagaggcc cccatggaca
     5701 tgccctgaac tttcctcccc tcgggcattg ccaaggaagg agagggctgc tgcagctcaa
     5761 gtgctccatg gtaagaggaa actgtaagat gatggggatg gggagggtcc ccacaaagcc
     5821 tggagccgtg tcagcctttt tcctgcaccg gaaaccacag gaaaccaggt cagtgcgtgg
     5881 ggctgctccc tctgctccag agctcggctg tcagctctgg ggcttcctga acaagccgta
     5941 tcatttagtt ggtcttcaaa gatggagagc actttgacag ctggaaaggg ctagtgggca
     6001 cagagagaag gctgggcttt agtgggaaga cagccgggtg gggagcccaa gtgcatgtgg
     6061 ggtacgcagg aggcttattt gtgttttata atgtttactc atttttgact tcagcaaatg
     6121 atgccccaac cacttcttta tcgtcagcat tcagcgtaga caaatggaga gtaactgttc
     6181 atttctcaaa gagaaggatg tggaactgtc tgtggcagag tatactaatg taatatgtgt
     6241 gtcagatcgg cagtatttga ggtgcaggag gggttaagaa atgggtgcac ctccacaaca
     6301 aggtgcaggt ccctttcctg ttaactgtgc cacaccaaga gcctcctgtg actccagggc
     6361 agtaaggctt tttacagagc aagcaagtgt tcctcagtgc cctggctcag gagaaggaat
     6421 gtgaccaagc ctgctggtta ggatcaagga ggagcttcgg ggcagggtgg atctagggca
     6481 gggttaggtg agagcgcctg ctggtactgt gtgcttctca cagctccagg gccaggccct
     6541 gctgccctct tgcagacagg aaagacatgg tctctgcgcc cggatcctac agaagctcat
     6601 ggggagcccc agactggcag ccttgctcct gtctctcccg ctactgctca tcggcctcgc
     6661 tgtgtctgct cgggttgcct gcccctgcct gcggagttgg accagccact gtctcctggc
     6721 ctaccgtgtg gtaaggtcct gctgccaagt gaaggggcac ttgcttggca cagactagaa
     6781 cccctctttc tcttgcctgc cctttcctac ctctgaaatc ctgggctctt agtggcagcc
     6841 tcagtgtgtc ctctatgtgg tcttgaaggg atggctaagg ttctggtgag cagaggtcct
     6901 aggcagggtg ggactgagag aaaaacagct ggatggatgg actgagctga gggtctcaga
     6961 cccagatggt tggatgcaga gtgcagggag aatgcacctt atgcgactcc cttctaatga
     7021 ctcctctctc ctttcttttc ccttctgggc cctgtatctt caggataaac gttttgctgg
     7081 taagtcttat ttcctgtcct gctcctcttc cctgggctcc ccagtggtcc aggacccaga
     7141 gagccatcct tggcagtttt ggttcccaac ccaaggagtc tgctatcagt aagaaagtgc
     7201 aggactagtc tagattcctc agattctata aatccttagg cagagctctt cactcctcta
     7261 tctgaccttg gcttctgagc accagagtgg aggagaaatg actagaagat gagggccgtg
     7321 ttgcttcctg ccaatgggga agataaaatg agcaacatgg ttaatctcca ggcatccacc
     7381 caagtggctc ttcctatcac tggcccctca ctctatcagg aacaatactc ttttatactt
     7441 tatgatacat tcaactttac caagctttcc cagatgcatt gcattactta agactcttga
     7501 tgaatgagca gtctggcacc tgggtctaat aactttctca agatcatact caaaagattt
     7561 atcaaagctc agccctaaat tcagttggaa ggaggtaaga gggacctcct tagacccaac
     7621 tgtttctctt tttgtttttg ttttgagaca gggtctcact atgtaatacc aaagcttcca
     7681 tcctttaatc ctcctgtctc agcctaagca atgggcttac agccatgtgt ctctatgctg
     7741 actttccttt ctctgacaca ggaagttttg ctcaaacccc ctgccctact gtcctagcca
     7801 caaagccttg gcactggtct tgctgtttgt gtctggtttc tggtttgtgt ctggtgtctc
     7861 attcttcagg taggaagagc agctttgagc cattcccagt ggaggtatgg attagggaca
     7921 gttgaagggt aagtacctgg gctgacacag tgtctgttgt catattggaa caggtttttg
     7981 tcttgttaac ctcaacaggg acttagagca tcctaaaaag tacatcacaa caaatacttt
     8041 tgagacaact gtatcattgt ggaggtgcca tctccacaaa gggcatctat ctccaaaact
     8101 gtgtgttcta tggacctgct gttgttactt ggcatctgta aagtgggatc aatttaccct
     8161 gctgtttcat tgcctcactt ggtacacacc gaacaaacag cagttataag ggggtagaga
     8221 gatggctcag tgattaagag cactcaatgc tcttgcagaa aagagttcaa ttcccaggat
     8281 ccacatcagg cagctcgaaa ctccacctcc agggaacctg atgtttctgg ccttcaagca
     8341 cctgcgctca ttcgctcatt agcacccacc tatacacagg cacacacaca tgcacataat
     8401 taaacataaa gtcacaaaac gttagtttaa attttttatc attagaaatg atgctaccat
     8461 gcctttaatc ccagcactca ggagaaagag gtaagcgaaa ctgcattagt ttgaggctag
     8521 cctggtctac aaagtgagtc caagacagcc aaggctgttt acactgagaa atcctgtctc
     8581 aaaaaaaaaa aaaaaaaaga cagaaatcat gctacagcta cagccagaca ctggtggcac
     8641 aggccagcac tcaggaggca gaggcaggaa gatatctaag ttcatagtta acctggtctg
     8701 tagagtaagt tctaggacag ctaaagctac atagagaaac cctgtcttga aaaacaaaac
     8761 aaaacaaaac aaaacaaaac aaaacaaaac aaaatcatgc tacagtgaat acctaccttt
     8821 gtggaagtgt gtgtgtatct tttttttttc tacttaagaa tttttaaatt gttttattta
     8881 aagtgtctca ttgaggctag agagatggct ttttataatt ttataattgt tataaacact
     8941 tgtgctcttc cagaggctct agacttgatt cccagcaccc acatggtggc ttacctctaa
     9001 ctctacagtt ccagagagtc cagtgtcttc ttctgatctc agtagacacc aggcatgcac
     9061 atggtgcaca tacatgtgtg caggcaaaac atccatatgt gtaaaataaa ataaataaac
     9121 ctaaaattgt ataaagaaaa gaaattcctt ttatttatta ttttaaaatc tcataacgca
     9181 tacactctat cttgatcaaa cccacctaca gaaggcgcat aacgatacca cgatatcaac
     9241 aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg aagttcctat
     9301 tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca cttgccccaa
     9361 acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg ttgtggggaa
     9421 gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat
     9481 gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag gtcagccccc
     9541 cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa ggggagatgc
     9601 cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac aaacttggcc
     9661 tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga tccccttgta
     9721 gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc tactcttttc
     9781 ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga tagacttgga
     9841 aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc tgggcattcc
     9901 ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct acatctgcca
     9961 atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg gcacatgtcc
    10021 agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt gggcgggtca
    10081 tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga
    10141 taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg acaaccaggt
    10201 cccaggtccc gaaaaccaaa gaagaagaac cctaacaaag aggacaagcg gcctcgcaca
    10261 gccttcactg ctgagcagct ccagaggctc aaggctgagt ttcagaccaa caggtacctg
    10321 acagagcagc ggcgccagag tctggcacag gagctcggta cccggaagat ctggactcta
    10381 gagaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
    10441 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    10501 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    10561 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    10621 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    10681 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    10741 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    10801 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
    10861 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
    10921 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaaccatgg
    10981 aagatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    11041 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    11101 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
    11161 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
    11221 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
    11281 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
    11341 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
    11401 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
    11461 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
    11521 tgatggtgct gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
    11581 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
    11641 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
    11701 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
    11761 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
    11821 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
    11881 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
    11941 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
    12001 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
    12061 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
    12121 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
    12181 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
    12241 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
    12301 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
    12361 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
    12421 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
    12481 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
    12541 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
    12601 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
    12661 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
    12721 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
    12781 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
    12841 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
    12901 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
    12961 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
    13021 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
    13081 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
    13141 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
    13201 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
    13261 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
    13321 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
    13381 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
    13441 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
    13501 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
    13561 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
    13621 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
    13681 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
    13741 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
    13801 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
    13861 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
    13921 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
    13981 aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat
    14041 aaccgggcag gggggatcta agctctagat aagtaatgat cataatcagc catatcacat
    14101 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    14161 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    14221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    14281 tgtccaaact catcaatgta tcttatcatg tctggatccg gaataacttc gtatagcata
    14341 cattatacga agttatgttt aaacggcgcg ccccggaatt cgccttctgc aggagcgtac
    14401 agaacccagg gccctggcac ccgtgcagac cctggcccac cccacctggg cgctcagtgc
    14461 ccaagagatg tccacaccta ggatgtcccg cggtgggtgg ggggcccgag agacgggcag
    14521 gccgggggca ggcctggcca tgcggggccg aaccgggcac tgcccagcgt gggcgcgggg
    14581 gccacggcgc gcgcccccag cccccgggcc cagcacccca aggcggccaa cgccaaaact
    14641 ctccctcctc ctcttcctca atctcgctct cgctcttttt ttttttcgca aaaggagggg
    14701 agagggggta aaaaaatgct gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc
    14761 aatcagcgtg cgccgttccg aaagttgcct tttatggctc gagcggccgc ggcggcgccc
    14821 tataaaaccc agcggcgcga cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac
    14881 agagcctcgc ctttgccgat cctctagagt cgagatccgc cgccaccatg attgaacaag
    14941 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    15001 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    15061 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
    15121 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    15181 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    15241 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    15301 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    15361 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    15421 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
    15481 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    15541 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    15601 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    15661 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    15721 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    15781 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    15841 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccccgga
    15901 tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag aggttttact
    15961 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    16021 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    16081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    16141 tgtatcttat catgtctgga tccgggggta ccgcgtcgag aagttcctat tccgaagttc
    16201 ctattctcta gaaagtatag gaacttcgtc gagataactt cgtatagcat acattatacg
    16261 aagttatgtc gagatatcta gacccagctt tcttgtacaa agtggttgat atctctatag
    16321 tcgcagtagg cggcttcccc tgaataactc ccaaaacatc cctgtcccat attcatatct
    16381 ttttaaaaaa gcatttatgt tttgtgtagt aatgttttcc tgtatgtgtg tgtgtgtgtg
    16441 tgtgtatgta tatattacat ccatgtagtg cccatgggag catcagatac ccccagcact
    16501 ggagttatag atggttgaga gccaccacgt gggtgctagg aaccaattct ggatcttcta
    16561 agaacagcaa gtgtcacatg tgtccctcca cgttttgaga cagaggctca ctgtgtagtc
    16621 ctgactcact tggaacttgc caagtacacc aggctggcct caaagtcaag ggctctgccc
    16681 gtctctgcct cctgatacaa gtataaaacc acaccttgct ctgttgtttc ataattcact
    16741 gagtccaatc agcggggctg cctactgggt attgactgat cttgctggct tgaccttgca
    16801 caggtcttgc gcaggtgatc accctgcagc gagtgaacac aaccttcata gtgtgtctag
    16861 aagacttgag aggactcctc ctctttttct gcctcttaca ttctttctac cttttcttct
    16921 gtgttctctg agcctcaata ctttttcact gaattataat ttttgccacc aagaatctga
    16981 atatcatttc tttccttgat ttataataaa tttctaccca atattggatc aagtctcagg
    17041 aaagacagag gccatgaata cccagataag aagtttgttt ccctcctaca ggccttcagt
    17101 ggggctggtt ccctctcttg gtgaggaaat ctaaaagtcc tcctaaattt gaagactatt
    17161 ggaggcacag gacaccagca tccttccagg taccctgtaa tgtatggctc taccctatgg
    17221 ttccaacccc tttctttttt gccctttact ttttctccat cgacaaaagg gagtttgaaa
    17281 atgtaaacat tacacagcaa gggcaactga ggccaaaaga acttgagcaa ttggcctgtg
    17341 gcctctcaag ttccaaggca gacttcctgg ctcctggctt aacaccactt tgttctctgc
    17401 ttgcagagga agctgctagg cagcccttcc ctgtctgagg aaagccatcg aatttccatc
    17461 ccctcctcag ccatctccca cagaggccaa cgcaccaaaa gggcccagcc ttcagctgca
    17521 gaaggaagag aacatctccc tgaagcaggg tcacaaaagt gtggaggtag gcatgatggg
    17581 tgaccggaga tggtttcatc cttacctgag ccagggtcac cgagtcttcc ttctccctag
    17641 gacctgaatt ctcctttgat ttgctgcccg aggtgcaggc tgttcgggtg actattcctg
    17701 caggccccaa ggccagtgtg cgcctttgtt atcagtgggc actggaatgt gaagacttga
    17761 gtagcccttt tgatacccag gtatgtagtg ccttgacaga tggcacaatt cctgagcaaa
    17821 gagactatct tacaattcct aagtaaatgg ggggaggggg gaaactaagg cccggacaga
    17881 gagtaattgc tcaaaactta tatcatgagg tatatagaga tggcgcaacg caattaatga
    17941 taacttcgta tagcatacat tatacgaagt tatggtctga gctcgccatc agttcaaagt
    18001 tgtgggctat gttacttgag aggagagacc agaaagaagt aagtatggta tatactccca
    18061 gggagagaat ccttagagac tagggctgcc cagttggtgg agtgcttgcc taccgtgcat
    18121 ggcctctggc attgtacaga tggggtggga ggtagaaata ggagaatcaa gagttcaagg
    18181 ttattattgg agatacaatg agtctgaggt cagctttggg gtacatgagc ccctgcctta
    18241 agaaaaacaa caacaaaagc tttgttattt tctcaagagg ttgtttccct agtaacttga
    18301 caaaatggcc acatgcatct tggtactcgt ctctcgtacc ttttgtgtag ccctcaggaa
    18361 attatatccc ctaacccccc cagaccaggt cctagcagcc cactacaacc tccaatcatc
    18421 acagatgctg ctgtgcatag cttggcctca tgtgcaaaga ccgaagccat atcctagagt
    18481 ctttccgtct cttcttgtct acaatggcta taccttctaa ctccgtggtg atgctagcta
    18541 gcctttagta gagttgatgc ttctaacacc ctacatggca tataactgga actcaagaat
    18601 agtatattcc cttccctaac cccactcctc ataaaatatc ccaagcaaat ggccagagta
    18661 tagaaccaag atcctatgag aaaaagaagt aagttttata tagtgggaaa ataaggtata
    18721 ttgtctgagg tcttagcttc ctcctacttg gtctacaata tccatgcttt gtcttaggtg
    18781 tctctttttc tcttcttttg tggttttatc aacagaaaat tgtgtctgga ggccacactg
    18841 tagacctgcc ttatgaattc cttctgccct gcatgtgcat agaggtgaga ataagggaat
    18901 ttgggctaat catggctatt tgcatagtca tatctccagt aaatcaattt acaaaggacc
    18961 agggatggat ggaaagacag caagccttac agcaccaaag agaaactcca acgggaccat
    19021 ggctgcttag gaactggtgc agtcttggct gtgagccaca gacgctcaga ggtgggaggg
    19081 tcctaggcag ttccacctca gccctgcctg gatgttcttg agagtcaagg ttgcatattc
    19141 ctctcaaagc cacgccctct ctagccctcc gttgtagtcc tccatctttc cttataagtc
    19201 ttaagataaa gatgttttgg acatcactgg gctaagagga gtcctgaaag aaggatagag
    19261 acccagggag gcaagaaact gccagagacg acgacagcac gtggactctc agggagtcca
    19321 gaatgaggaa tgacacaggg cactgggtac ggtgagaaag cccagaatgg gtgtcagcaa
    19381 agcctaaaat tgagagatgt ggcccacagg agttctctgt tgctgggaat agttctcttt
    19441 ggagtccaga ggacaccgcc gtattcgctg ctgtttcctc ggctctagag ccctaactcc
    19501 acagagctcc ctgattgatt agtgtcctga ggctcagggt acttcaccat agtgagctag
    19561 agccagaact aatgatgttg gaaaagctat ttccttgatg ctctctatgc tttgtcttcc
    19621 caggcctcct acctgcaaga ggacactgtg aggcgcaaaa agtgtccctt ccagagctgg
    19681 cctgaagctt gtgagtgttg tattaatcct gatgcaggat gagtgttctc cttccaccct
    19741 ccctccctcc ccgacctgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg agagagagag
    19801 agagagagag agagagagag agagagcaga tggcatgagc acatttcttt ggggggagca
    19861 gtgtctagcc atcaacctgc agatgagctc cctaagtgct gagcgttgtg aatggaggag
    19921 tctcacccag ggagtggtga gtgaaaggac cacctagaga cctaggctca cttcccagtt
    19981 ttgccataga ttatccccag attcccccct ttccttcatc agtaaaatcc caaactattg
    20041 atgaggcttg aaggaactat ttttcagcaa agtggctggt attgtctaga gtgctgtgtg
    20101 cacagcaccg accttaagag aaaaacactc agatacaggt aacaaagcct agtcctcagg
    20161 cagggttatc ttcaagggct gtctgcagga cgtcgtcatg gggaaggagg agcttgagac
    20221 actgttgtga gcagagaggg tctcaggggt gccctcgcct gagctgagct caggaaatgg
    20281 acaggattca gaaagaagag tcagggaaga aggcatttag cttctgagag gagcccgcac
    20341 agattttgag tacgggatgt tttgagtaca gtgtggagac ctggaatgga aagggaaagg
    20401 caagaaggcc aggtagaaag gtaggtaggg aaagtggagg catgctcacc tgctgagcac
    20461 ctttgcgcag gacttgtgct ggaactttgg tattttctcc tgatgccctg gagaggggtt
    20521 ggggagatgg agctacagct gggctatggc ttcatccttg gacctgccac ctcccagatg
    20581 gctcagactt ctggcagtca atacgcttca ctgactacag ccagcacaat cagatggtca
    20641 tggctctgac actccgctgc ccactgaaac tggaggcctc cctctgctgg aggcaggacc
    20701 cactcacacc ctgcgaaacc cttcccaacg ccacagcaca ggagtcagaa ggagtaaggg
    20761 cagccacctc cctccaggac tgagatgggc aggggcaggg cccagggacc ttctccctga
    20821 ctaagtcccc tctccctaac agtggtatat cctggagaat gtggacttgc acccccagct
    20881 ctgctttaag gtacaaccat gggcaggggg ctggccgtgg gagaaggggt gggactgaac
    20941 cctgctgacc tgctatgcct gtgtttctca gttctcattt gaaaacagca gccacgttga
    21001 atgtccccac cagagtggtg agtcagtagg aaatcccccc tcccacatct cacccccact
    21061 cgcctgtgac ctccaggtcc agaactaccc agctctgatc acctcccaca aacaggctct
    21121 ctcccatcct ggactgtgag catggatacc caggcccagc agctgacgct tcacttttct
    21181 tcgaggacat atgccacctt cagtgctgcc tggagtgacc caggtttggg gccggatacc
    21241 cccatgcctc ctgtgtacag catcagccag gtagggccta gctagggttt gcttcccacc
    21301 aggtcctctt ggccaatgcg tatccctagc cttcctgtta cttcagtgag gcagaccctg
    21361 cctgcgtggg tggagccaca aagcccccat cccggcatct gtcatagatt attttcttga
    21421 ttaaccttgg atgactagcc ctctgtcaat ctgtgggacc ttcaggaggg agttcctaag
    21481 cctttcagtg cctcagttta cagagagagc agtgtccctt cttcagctac tctccagccc
    21541 actgacgcca ctgctgcatt ttgtgtgtgt ggtgtacgca tacgcatgtg taggtgtgca
    21601 aacctgtggg tgtaaacaca gagaccagag gtcttctatt catctctcta cattattcct
    21661 ttgagaccag gtctctcagt gaacttggag cttgctgttt tcagctacgt tggctgtcct
    21721 gggattcacc tgtctctgac ccagtgctga ggttgcatgg atatatggct gtgcttggct
    21781 tccctgcacc tccgggcagg gcttctctat gtagccctgg gtgtcctgga actcagtctg
    21841 tagactcaga gatccgcctg cctctgcctc ccaagtgctg ggattaaagg tgtgcactac
    21901 caccaccacc accaccacca ccaccatcac caccaccgag ctctttaaaa aaaaaaaacc
    21961 aaaaacaaaa acaaaacaaa caactctgga tacttagggt ttaaattcaa gtgctcatgt
    22021 ttgcatagac aatgca
//