LOCUS       1001421#LoxP#L3L4_pD223_DTA_T_spec        19632 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_lncRNA_xpA3_BactNeo
COMMENT     design_id: 1001421
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000181458
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10635
                     /note="5 arm"
     misc_feature    10659..10692
                     /note="Downstream LoxP"
     misc_feature    10636..10715
                     /note="synthetic loxP region"
     misc_feature    10716..11222
                     /note="Critical Region"
     misc_feature    11250..11274
                     /note="B1 Gateway"
     misc_feature    complement(11287..11291)
                     /note="SwaI blunt end"
     misc_feature    11281..11286
                     /note="remains of XmnI end"
     misc_feature    11292..11298
                     /note="unique 5' site"
     misc_feature    11299..11300
                     /note="buffer"
     misc_feature    11349..11350
                     /note="Buffer"
     misc_feature    11351..11357
                     /note="For 5' end of rabbit b-globin(BbvCI replaces
                     NotI)"
     misc_feature    11301..11348
                     /note="FRT "
     misc_feature    12528..12531
                     /note="PmeI for 3xpA"
     misc_feature    13282..13283
                     /note="NdeI for 5' Bactneo"
     misc_feature    15461..15494
                     /note="LoxP"
     misc_feature    15496..15500
                     /note="unique SacI site"
     misc_feature    15501..15506
                     /note="SalI for cloining into XhoI end"
     misc_feature    15401..15406
                     /note="unique SpeI site"
     misc_feature    15407..15454
                     /note="Frt"
     PolyA_signal    11358..11370
                     /vntifkey="21"
                     /dnas_title="pA %DF-globin"
                     /label=pA %DF-globin
     Intron          11362..11370
     misc_feature    11371..11372
                     /note="SanDI cloning site for rabbit B-globin"
     misc_feature    11358..11372
                     /note="rabbit betaglobin"
     misc_recomb     13289..13320
                     /note="rox"
     polyA_signal    13329..13566
                     /note="SV40 pA"
     misc_feature    13568..15355
                     /dnas_title="for bActNeo"
                     /note="for bActNeo"
     misc_feature    13574..14094
                     /note="human beta actin promoter"
     misc_feature    complement(13648..13667)
                     /note="hBactinNde_R primer"
     misc_feature    13970..13989
                     /note="betact5"
     misc_feature    14120..15105
                     /note="neomycin resistance "
     misc_feature    14289..14308
                     /note="neoPstI_5"
     misc_feature    14626..14644
                     /note="neoSphI_5"
     misc_feature    14352..14934
                     /note="synthetic fragment mutates RsRII"
     misc_feature    14764..14764
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    14919..14938
                     /note="neoBstB1_5"
     misc_feature    15170..15193
                     /note="RAF5"
     misc_feature    15112..15355
                     /note="SV40pA"
     misc_feature    14986..15005
                     /note="Neo_F"
     misc_recomb     15357..15388
                     /note="rox"
     misc_feature    13284..13287
                     /note="NdeI"
     misc_feature    15390..15394
                     /note="XmnI site for 3' inserton of Bactpromoter_neo
                     BLUNT"
     misc_feature    11471..11490
                     /note="Bglobin_F1"
     misc_feature    complement(11468..11487)
                     /note="Bglobin_R3"
     PolyA_signal    11373..12515
                     /vntifkey="21"
                     /dnas_title="pA %DF-globin"
                     /label=pA %DF-globin
     Intron          11373..11934
     PolyA_signal    11986..12499
                     /label=pA Terminator
     misc_feature    11960..11979
                     /note="Bglobin_F2"
     misc_feature    complement(11961..11980)
                     /note="Bglobin_R2"
     misc_feature    12522..12526
                     /note="NsiI 3' cloning site for rabbit bglobin"
     CDS             12532..13280
                     /label=New_PmeI-x3pA_AscI
     misc_feature    complement(11453..11476)
                     /note="Bglob_LRPCR1"
     misc_feature    complement(11661..11684)
                     /note="Bglob_LRPCR2"
     misc_feature    complement(11282..11305)
                     /note="IRNA5_seq"
     misc_feature    15406..15429
                     /note="IRNA_LRPCR"
     misc_feature    15480..15505
                     /note="IRNA3_seq"
     misc_feature    15522..15542
                     /note="B2 Gateway"
     misc_feature    11223..15572
                     /note="Synthetic Cassette"
     exon            19255..19632
                     /db_xref="ENSEMBL:ENSMUSE00001139750"
                     /rank="1"
                     /note="ENSMUSE00001139750 5' fragment"
     misc_feature    15573..19632
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacaaggt tagggacacc gtgtcggccc cgggctcagg tgggcactat
     4321 gccccaggcg ggcgccagga cttggccctc tggccttctt ccttcccagc cacgagcagg
     4381 gccacgagca gggtcccggg cggctcccgc catccgccgc aacaatgtgg ccattgtttg
     4441 cgcgcgcgga gcgggcgggc gcgcaggtgg gaggccgcgg gcgctcgggc acggaatgtt
     4501 cccgggaccg ggcgcgcttg gcgccgcccg gacacgcggc ctgcgcggcc ccaccgggac
     4561 ggcggaacct cggaggggcg gggcgggaag ggatggggcg gggcagacgg gcgggggtgg
     4621 ggcggaggag ggagagacaa agaggagcag gggaggaaag gagggatgaa gagggtatgg
     4681 gaaggggggc aggggagaag aaagtggaga caagaagaag aaaaggaacg atggggaaaa
     4741 gagagaggaa gttaggaagg ggagaaaagg acagaagaga ttgaaaggag ggagggaggg
     4801 agggagggag ggagggaggg agggagggag ggagagagag ggataaaccg ggaagaaaga
     4861 ataagtgaga cacccagcaa cccagaagta tgagggccga gtaagacgag gaaagagcaa
     4921 gagttcaccc tcatacagaa aaaggactcc aagccctgcc aagtcagcag ctttaatcac
     4981 acccggggct ttgcctaatt acagggtgaa agccaggtct agaggtaggg agatacttaa
     5041 ctgtgtccca gggaaagcag agggcagaga caggctgtgc aactgtctct cctccaccca
     5101 tgccaacccc tcagagcctg gatcctggag gaaaaggctg gggggctgca gcaagactgg
     5161 ccaccggcct ctgagacccc gggttttacg ttcccttgag tgggaatcca gtctgtatag
     5221 ggaccaccct ctggccttcc tgagttctct cactggagga ctgggaccct ctgcacccaa
     5281 agaggcgatt caatcctagc cccgggctga taaattaaaa agttctcttt atttcatttg
     5341 ctggtttgag gttcattaaa ttttccacag attcgcagag acgaaagcag tcaataaact
     5401 cggacaaaag tgcccgaacc aaaaagggca gagaattggg aaaaggcaag ataaaagtgt
     5461 tatctgatcc cagatccgca ggcagcaaag gagcagggac tgccgcaaga atctcaatgg
     5521 gccatcaccc ttctcagcgt gcctcccgtt agtaccaact cctcattcaa cacaaaagcc
     5581 aaagcactaa aagtggtttt cttatgctaa tgttgaaacc cagcactcag ttaaaacaca
     5641 ctgtccagaa acaacctgcc tttttaaaag acacacatgt gaaacacaca cgaaagaaag
     5701 gaagaaagaa agaaagaaag aaagaaagaa acccacgttg attcgttcta tttaaaccca
     5761 agtcccgaaa aaagaaaaaa aaaattaatg ggtcctgtct ggtcacatct tgaaagagtt
     5821 atttcccagc tcatgtatga tacgtaacaa gccagaagga aaacctaagt agaaacaaga
     5881 cttcttcagt tgttggaagg attcaggaca gtaagcctct ccgagagaga ggtcatctcc
     5941 ctgggttttc ccaggattta aaatgtgtgt tttaaaagtg acacttttgt caccctcaaa
     6001 aatctaaaaa caatggccaa agttttcatt ttctaagtcc tttacatttt ccacaagcct
     6061 tcagatttgg aaaaaaaaaa aaaaaagaca agtgctgagt atggatggag ctgtgtttac
     6121 ttgaaagcca cagaatcata gagaaggtgg tcttctttac tctaagatcc tatcggtcag
     6181 ctgacagcca ctgctgctaa cctcagtcca tacctatcag caaaggacac cagagtactt
     6241 attctcttcc cctaagttct ttaaaaatga gagcacgctg ggcactaagt atctgggtag
     6301 cctggctgcc gacccagtca ccgactctac ccctcctggt gttctagaat ctgagaacac
     6361 agtctttagt tggcattcct cacatgtcca tgcctctgat gcttagtgaa aagaagaaag
     6421 gagggagttg cttccctggg gttccctttc aatgggccct ggcccagcag ctgcagctgt
     6481 ccacagtgaa gtccacctgg cctctggccc ctgatgtgcc agttaacagg ctacaggtag
     6541 acctcccacc caagaccccc gcctaaagac acagggaaag cagccccacc ttgttccctg
     6601 cggtttgcac cagcgaaggc cagctgcacc cacaaggttt taggactctg tttttgagct
     6661 ttaacaagcc agctgggtgt gaggggtcac agccgcattc acagtgcata caacaacctc
     6721 atggtctcat ttaaagggaa ctgctagtgt ctgggcactg aagttttccg ggagtggaat
     6781 gctgagggaa gaagagtact tagtgtaggc aaatatccaa ttttgtctgc ctgcttgcag
     6841 actgtcccgg attcactaat ccagccccag gggctgcact gtgtacaggg ctcttgtaga
     6901 agtacaaaaa aagagtgaag tatttgtgta tttttcaggc tgcagtgcaa acaagccagc
     6961 tacagaacac agcccctgag acccccacag cagctaggaa atgagcctgt gaccagtaac
     7021 cacacacaca cacacacaca cacacacaca cacacacaca aacacaccct cttacaatta
     7081 tgcaactcaa aagcacaccc tctttgattg ctcagtgctc aatgttcttc caagcctgca
     7141 aggcccaagg aacatcctga gaaagtgaag ctaactccat atacacttca aggtggtcct
     7201 ccttccaagc aagcccggct tgaatttggg agaagacctc agtgagtctg aattctgtgc
     7261 atatttccac catctcagtt cccacctaag cacagaaaca gatctatcaa tcccagtccc
     7321 agttcgttca tggaggagtc cagccctggg tgggaagata cttgcttccc atcggcctct
     7381 cccacagata caggacaaag acaagagcag atctcctaca gggcacagta cacagaacct
     7441 tcttctgtgt gttgtgactc taagccccct agaatcagga aatacctcat gacatttggg
     7501 ggcatcctaa gtctccctaa acataattaa tgttaaaact catcaggtga gctttccata
     7561 cagcaggcag cgtctgagta gaaactgtat cctgaagagg ccatacccaa ctcttggaac
     7621 cctctagaga gaaggagctg gttggagaaa gggaaagcaa gatgtagaga ctctaggagt
     7681 gaagccttta gcaggagggg tctgcgtggt ttagattaga cattctggga ttagtgccca
     7741 ggatagaaca gaagctggga gaaagcaaga gcctcaaaac tcaaagcgag taagcatcag
     7801 gaatgtttcc tcctacttgg gggagaccaa tccaggtagg aacacaatgt attccctccc
     7861 caattccaga ccaactgagc cattgaaggg gctagtcccc cagacacacg gaagcctact
     7921 caaaaaggga aaccgcagcc aaggtgagga aatgggcggg gagggggctc gaaggagagg
     7981 tggctagaac aaacagataa catcagggag acttgtgaca agcccgccta ttctgcccgc
     8041 tatccccccc gtgctgtata ttttggattt caccgtttat ttacttttct tttttggcac
     8101 cgagcccccg gagcatgcca atacaatatt tgtttaaaca ctattttatg ccgcttgcga
     8161 gggaaacgtt tactttcaaa ttctccaaca tcccccagag cctcgtctgc aaacagcata
     8221 tgagcgtttt acaactgttc cctttatctg aagctgtggg gattaaacct ttacacgccc
     8281 agcgagggcg gaggggcaag gggcccagga catcgtgcgg ccaccgaaca gcaagatgaa
     8341 ggatgcccca gctggggaac agagaggtac agaacccatc ccttcccagt ctctgaagga
     8401 tgagactacc aagagaagtc tctcctaccc ctgctccaac tgggatcggc tctgggaatc
     8461 acaggacagt tacacacaca gacacacaca caaagcctgg aaggagaggc gccctggggg
     8521 atccccacaa actccttgcg tctctgtagc caggtcactc gaggtgaagc gcaagggctc
     8581 catccttagt gtcctcaagc tctaaaagtc aggtttccta ggaaatgaga gcaattgctg
     8641 ccgggctgaa aaggagccca gttgggtgga cttccagctc ccggcgcggc gggagccctc
     8701 tcgaactcac tcaagcacac ggtccctccc cccaccccca atcccggagg aaaaaaaacg
     8761 ctcccctctc tctaaaccct taataggatt ggcgcccaac tctgtccgcg ccgctcctca
     8821 tccatcacac acagatcaag ttgcagtagt ggatttttca aattcctcag caaatatact
     8881 tgttgagaga ttgcttgggg ggtgttaagt gggggctagg atgaaagcga ggagtagggg
     8941 gaaccgatcc ctcggcgaag gattacgcgt ggatccattc tggaagtcaa ggggatctgg
     9001 agacctagga taagagagaa aggtaaggaa gccagaaccg aggtccagag actggcggcg
     9061 gagggaaggg gcaacagggt tcaacgagag ggaaagaagg aagaaagaaa agactggctc
     9121 ctggacgcgg actcgcagcg cggagaagta gagttgcaag tttccaagtg gccggcggcg
     9181 gttgcagcac accgcacagt cccacaggcg cgctcccagc tgcccatcta agggagcttc
     9241 ggcgcacccc agcccccacg tcgcccccaa acagttccag gctccaagcg gtaccaccgg
     9301 tcaaagaccg gcctacccga gcaacccaag aacaagaagc acttcccctc aagtggaggg
     9361 ctgtggtaat aaaatcgacc ccggccacga gttctgatcc ccaagatcct ccttaagccc
     9421 tgccgctgct ggaccagggc actaatcagg gttgccttct ttagccggcc cccctaaaat
     9481 cagtgtcacg atctcgattc cggtaaacgg atgagagaca ctccaagacc gccacagcat
     9541 ctcagccgaa gctggccagg ctaggaagtg cttcgccacc aagagtaagg ggaggaaaaa
     9601 aaaaaaaaga aagaaagaaa gaaaagaaaa aaggaagggt gggaaggcca atgcaacttt
     9661 accgaggaga caaaagtttg cctctagcat gcaggtggcc tctcgggcgc cgggcagtgg
     9721 ttgcagggcc ccatcccaac ttggctggag tggggctgcc gagtgcgggg gctccggctg
     9781 ccggggcggg gctggccagg cagaggaggg gaccaaggcg ggcgggggac tcgggcagtc
     9841 cctaaggaca ccaggccagc agctccgggc ggctgcgatg ctacgcacga cccgtgctcc
     9901 ttcctctggc tcctcctcgc cttcctccgc gtcctctgta gccgccgccg ccgcctcccg
     9961 ctcctcctcg aagtctcatt gatgagagga gtttgaaaaa aagtccgcgg agccgtgctg
    10021 cctcggctgg cgaacgccgg cactcatgca atccatcagc gtctctggga tggcgcagcg
    10081 cccccgcgcg ctgagcttgg gcaaaccctg atcgtcgcgt ccttagcttg cccgccccgc
    10141 cgcgtgcacc ctgcctctga ggctgggggt ggggggacac caccctggag ggggattgcc
    10201 agcgggcgaa cacagactct cccccacctc agctccagca aggaaagcac cccttcggaa
    10261 agttccagcg ggggaccaga gaacccagga gttagggagt gggggtgggg ggtgacgacc
    10321 cagacgccac tctggaggag aagagctggg actatcgcag attcttccaa cccagttcgg
    10381 aggaaataaa gcacccctac cagaaagttc cagctacagg agcacacgcc agtctcaaac
    10441 actttttcta aggcgccggg ttggggggtt gctaactcaa gtcagatgag ctccggagct
    10501 cgggcgctgc gactgctggt ttcaatatct gaactccccc tttcttccca accccctcag
    10561 ccctccgctg gttgttccca aaactcccgg atcctctgtg cgccgtagca gaaggaagac
    10621 acgtgggttg ggaaggagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt
    10681 atacgaagtt atggtctgag ctcgccatca gttcactgga ttcacgcagg ccctcgtcgc
    10741 ccgggcagct agtgcaggtg cccggggtcc caggcgcccc tcgccatggc tcgctcacgc
    10801 cgccatctga gagcacgccg gcgacaagtg acgccaacct taggcttgct gcttatgttt
    10861 tgcagtgaga ggggtctttc tttctttctt tctttctttc tttctttctt tctttctttc
    10921 tttctttctt tttctttttc tttttctttt ttttctcttt tttttttaat cccggagact
    10981 ccattgaagc tgctgcagcc agatggaagt aatcagaggg agaaggaaga attaggctca
    11041 ccgaagttgc aggccaaagt gtagatgagg ctgttcatga aagcatttgc ttaagtagcc
    11101 ccggctttcc atacagtcca gaactgtcca ctccaagaag caagcaggca gcaaatggcc
    11161 gtgccctccc aagccatggg actcagcttc ctctcctggg ctctgctctt agaaagctgc
    11221 ccaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc
    11281 ggaaccaaat tgctaagcag gaagttccta ttccgaagtt cctattctct agaaagtata
    11341 ggaacttcct cctcagccag ggtgagtttg gggacccttg attgttcttt ctttttcgct
    11401 attgaaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
    11461 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
    11521 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt tttcgttaaa
    11581 ctttagcttg catttgtaac gaatttttaa attcactttc gtttatttgt cagattgtaa
    11641 gtactttctc taatcacttt tttttcaagg caatcagggt aattatattg tacttcagca
    11701 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
    11761 tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt aatcatcctg
    11821 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
    11881 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg
    11941 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac tcctcaggtg
    12001 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
    12061 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
    12121 acttctgggt aataaaggaa atttattttc attgcaatag tgtgtgggaa ttttttgtgt
    12181 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
    12241 gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt ggctataaag
    12301 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
    12361 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
    12421 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
    12481 catagctgtc cctcttctct tatgaactcg actgcacgcg tatgcatgtt taaacttaat
    12541 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
    12601 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    12661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    12721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12781 ggatcctgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
    12841 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    12901 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12961 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    13021 tctggatcct gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
    13081 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    13141 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    13201 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    13261 atgtctggat ccggcgcgcc ccatatgata actttaaata atgccaatta tttaaagtta
    13321 ggatatctaa gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
    13381 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    13441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    13501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    13561 atgtctcgaa ttcgccttct gcaggagcgt acagaaccca gggccctggc acccgtgcag
    13621 accctggccc accccacctg ggcgctcagt gcccaagaga tgtccacacc taggatgtcc
    13681 cgcggtgggt ggggggcccg agagacgggc aggccggggg caggcctggc catgcggggc
    13741 cgaaccgggc actgcccagc gtgggcgcgg gggccacggc gcgcgccccc agcccccggg
    13801 cccagcaccc caaggcggcc aacgccaaaa ctctccctcc tcctcttcct caatctcgct
    13861 ctcgctcttt ttttttttcg caaaaggagg ggagaggggg taaaaaaatg ctgcactgtg
    13921 cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg tgcgccgttc cgaaagttgc
    13981 cttttatggc tcgagcggcc gcggcggcgc cctataaaac ccagcggcgc gacgcgccac
    14041 caccgccgag accgcgtccg ccccgcgagc acagagcctc gcctttgccg atcctctaga
    14101 gtcgagatcc gccgccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
    14161 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
    14221 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
    14281 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
    14341 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
    14401 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
    14461 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
    14521 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
    14581 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
    14641 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
    14701 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
    14761 ggccgaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
    14821 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
    14881 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
    14941 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
    15001 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    15061 ctcatgctgg agttcttcgc ccaccccccg gatctaagct ctagataagt aatgatcata
    15121 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    15181 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    15241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    15301 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccctaac
    15361 tttaaataat gccaattatt taaagttagg aatttaattc actagtgaag ttcctattcc
    15421 gaagttccta ttctctagaa agtataggaa cttcgtcgag ataacttcgt atagcataca
    15481 ttatacgaag ttatgagctc gtcgacgtcg agatatctag acccagcttt cttgtacaaa
    15541 gtggttgata tctctatagt cgcagtaggc gggcgggact tctttatatt tcttcctgag
    15601 gtctagttcc tttgtattgc ttagcctgcc atagggtatc ctccttttaa aggtggagtg
    15661 actgatgcct gaggacgctt agaaacttgc ccacaataat ctaaggtcgg tccatttaaa
    15721 tagaggtgtc ctgttgagaa tgttggtcac atctctttcg agcccctcct tctcaaagca
    15781 agagaaccaa gcctccagac caattccacc ctgccttaga gaaagctcct tctcagccca
    15841 tgttactcac tgattggatc actgttggtg gacggcacag tttacaccgt gggctctccc
    15901 ttagctttat ttgtttctgc aagtcagtat ctattttatg aaatagccca aggcattcca
    15961 aatgctatgg atactctcct tccgaggtcc acttccaaga ccctgagttt tacatttaaa
    16021 gtttatattc cacatattga ttttgtggga cagggtgtga ggtcacagtc ctctggcagc
    16081 agtcagagga cagggcagag ggggctgttt ctctccttct gtcatgtggg tgctgaggac
    16141 cccacccagg ctcgcagcaa gcacctttgc tggcctagtc ttttactgac cttgacttac
    16201 tttttaacag aaggggaagt gctgtgtccc attcttcctt tcttgttacc ttccttcttg
    16261 atctagagcg gggaagggca gagatgctac aaggatttga tgctaacttt gtcttcccca
    16321 aagtcatctt cccaggttgg aagtcagggg gagtgggggt ggggaggtga caaggtgttc
    16381 aaatggaaaa ctttctggtg tggcgataca tggtggggtg tgacaggacg tttttctgag
    16441 aagatgcagc ttgtgtctac ttctctgccc ctcacaccca gggaggaacc cacaatagtc
    16501 caaagtgtgg ataccactgc agtctgactt ggtgaaccca tgaggtttgt gtcttacagg
    16561 ctgtggatga ggagttactt acaggaacgg aaatgactca aagaccagct gcacactgaa
    16621 gcccacccca gtgtggggac agcttaccaa gctgggaacc tgtgcaccag gcggctcaac
    16681 agactggaga gtgtcctgtg ctctccatgc ctcagtgggg ctaaacctct tccagacaac
    16741 cagccgctgt ctgcttcttt caggctgctg ctctggtgtc cagactttgc ccctttggct
    16801 tgtctgagcg tctccttggc agcccaattt cttcatgtga gggagactct caacttttat
    16861 tatttatgct ggcagggagg ggcctagtga ttctggtaag ttccagggcc ttcctgaagc
    16921 tattttgagt tgtttacctt cctgttcaaa gagtttccct gtagggtgga ctctctttca
    16981 atctcagagg aaacctaagt taggcaagtg ccttcggtcc taagactcca gaggcagaca
    17041 gacctctgag ggtgagaagc tagcctggtc tacactgcca gttccagacc agccagggtc
    17101 atacaatgag accctgtctt taaaaacaaa aagaataata ataaaaataa aaccttacag
    17161 aagtcagtac ggaaaatgtg ggataaaggc catggagaag ggccagaagt cagggcactt
    17221 ggtgtggatc tattctgact tgattggatc cctaggactc acgtaaagtc aggacagact
    17281 ccagcagatt gtcctctgac ctctacgtac catctgtgac atttgagccc cgcccccaat
    17341 acatgtaaac aaaccccaga aaaacagaaa gagatatctg aaggaggctg gctttttcct
    17401 ttaaatgtca gaacatcaga tggtcactcc tcccggctgc ttcttctcac tcccgctgag
    17461 ctttccgaac atagacagca aaagtgaacc ctctccctcc cttggctgca tttaatgacg
    17521 ccattcatat gggccctgtg aagccaggac tcctttctcc tggctggaat attagcggag
    17581 tcagctgatt acttcattaa agcttgtctc tgtgggtttc tttgcgttgt aaagtttgtg
    17641 gagaaatcta aacaaatcag catctggaga aagagggaga gattgctctt tctctgtttt
    17701 tcttctcgga ccagctcaac tcctgccaga atccaggccc tcaccaaacc ttcctggctt
    17761 attttggcaa gacagtggta gcagagatga aggtggaaat gtttggggtt tgaggggagc
    17821 tgagaaaaca gcacatccca ctgtctgcag atggtcagaa gaggtgctcg cttatcccag
    17881 agcttatact cagtgtaggg cattagtcaa gtccggcttt cttctgcagc tttctgtgta
    17941 gccagtgcta gtgtcgtgac acccaccatg gtgagtagca cccacagcat ccctccttcc
    18001 ctatgcttca tagtgataaa gatggacagg gatggaggaa gtggaaatgg cttttcttgt
    18061 ccttcttggt gatttgaata ggatgtttcc tgtagtctca tccactgcaa tgcttagtta
    18121 ccagtgaatg gaattgtttg ggaagaatta ggaggtgtgg ccttgtagga aaaggtctgt
    18181 cactgggagt gagctttgag gtttcaaaac ccaccaatac cctcttcttt tcctcctctt
    18241 ctctttccct ctcctcctcc tgccattatg ataatgaact aaccctctaa aactttatgc
    18301 aagccccaat caaatgcttt attttataat ttaccttggc cattttgtct cctcttagca
    18361 atagaccact gactaagaca ccatctgaat gggtgaaacc ctgttccccg gggaagctgt
    18421 ctgcaattca gagccttctg aaccatgttt ccatccccat atttgtgtgg gagtttaggg
    18481 agagggtaca ttaaaagaaa aacttcttga aggcaggatt ccctgttact ctcaaactca
    18541 ctatgtagcc aaggatagcc ttgaacttct aattttctcc ctccagccct gaatgctggg
    18601 gttctaggta tgcagcatga actatacagt ttgatgcatg caggggatga aatgtggtac
    18661 ttcctaagtg ctaggctagc tgtctactgc gctacgtgct cagtactata tgtcttagat
    18721 ttttatatga ctggcagtgt ggtttcttca agccattgtc accactcaaa ggtaacacat
    18781 caaaacaagg cattacaatg tctgtgacac tgtcaggcaa tgatatatct ttttttttct
    18841 ttttagggat catatgtttg tatgtacaga ggccagaaca cagcatcagt gtttttcctc
    18901 agaagccatc caccttcact ttgggagggg ctgtttgttt tgagaggtta cttttagatt
    18961 tagttatgtt tacgttctgt gcatgggtgt tttgcctgcc tgtatgtctg tgcaccacat
    19021 gtgtgcagtg cctgtggagg ccaaaagagg gtactggatc ctttggagct acagttacag
    19081 ataattgtgg gctgctattg agtgctggaa acctggccct ccacactagc cacaaacgtg
    19141 ccaactgctg agccatctct tcaggcccac ccttaccttt gtgttttcaa tcagggtctc
    19201 ttcctgagac ctgggctcac ccatgaggct ggcctggctg gccagcaaac cccagggatc
    19261 ctgtctctgg ctcccgcagt taagattgca tgttctatga cgttactaca ttcagctttt
    19321 tacctgggtg ctgaggcttg aaccagaatc ctcatgcttt ccaccagcta agctgcagcc
    19381 agcccttcat tgcagtctta tgagacagct gtcatttacc aaatcgtagt tagttacaca
    19441 gctgcaccag gaagcgtatc acccagagcg taatgacata aacacacaac ttcagacaag
    19501 atagacaaga aaagcaggag tttggctctg ttccacagtg ttggggactc agctagaagg
    19561 ctctgaggct ggctggcacc aagggatgga aatgaattat tctcaatata aatacaacag
    19621 agggactaga ga
//