LOCUS       KO-first_condition_ready_44797_MGI:1920708        23381 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector 1700055N04Rik targeting project(s): 44797
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_GT0_LF2A_LacZ_BetactP_neo
COMMENT     design_id: 467949
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000179433
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5007..5112
                     /db_xref="ENSEMBL:ENSMUSE00001053912"
                     /rank="1"
                     /note="ENSMUSE00001053912"
     exon            10038..10200
                     /db_xref="ENSEMBL:ENSMUSE00001094253"
                     /rank="2"
                     /note="ENSMUSE00001094253"
     misc_feature    4275..10352
                     /note="5 arm"
     misc_feature    10380..10404
                     /note="B1 Gateway"
     exon            11340..11374
                     /note="mouse En2 exon"
     misc_feature    10417..10464
                     /note="Frt"
     misc_feature    11337..11339
                     /note="Splice Acceptor"
     intron          10465..11339
                     /note="mouse En2 intron"
     misc_feature    complement(10533..10554)
                     /note="LRPCR LAR3_EN2"
     misc_feature    11240..11261
                     /note="seq primer FCHK2"
     misc_feature    complement(10533..10554)
                     /note="seq primer L1"
     misc_feature    complement(11663..11682)
                     /note="seq primer FCHK1"
     misc_feature    complement(10429..10452)
                     /note="FRT3"
     misc_feature    14443..14463
                     /note="seqlaczF"
     misc_feature    14658..14913
                     /note="SV40 polyadenylation site"
     misc_feature    14728..14751
                     /note="RAF5"
     promoter        14976..15496
                     /note="human beta actin promoter"
     misc_feature    14917..14950
                     /note="loxP"
     misc_feature    15372..15391
                     /note="betact5"
     gene            15522..16507
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16514..16757
                     /note="SV40 polyadenylation site"
     misc_feature    16774..16821
                     /note="Frt"
     misc_feature    16828..16861
                     /note="loxP"
     misc_feature    16572..16595
                     /note="RAF5"
     misc_feature    16754..16774
                     /note="PNF seq primer"
     misc_feature    11375..11558
                     /note="LF2A"
     gene            11559..14630
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    11354..11379
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11354..11369
                     /note="FCHK QC prefix region"
     misc_feature    11370..11373
                     /note="FCHK QC critical region"
     misc_feature    11374..11379
                     /note="FCHK QC suffix region"
     misc_feature    16877..16897
                     /note="B2 Gateway"
     misc_feature    10353..16927
                     /note="Synthetic Cassette"
     exon            17148..17258
                     /db_xref="ENSEMBL:ENSMUSE00001043838"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001043838"
     exon            17497..17617
                     /db_xref="ENSEMBL:ENSMUSE00001007281"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001007281"
     exon            17736..17821
                     /db_xref="ENSEMBL:ENSMUSE00001053426"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 3 ENSMUSE00001053426"
     exon            17934..18015
                     /db_xref="ENSEMBL:ENSMUSE00001063364"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 4 ENSMUSE00001063364"
     exon            18313..18699
                     /db_xref="ENSEMBL:ENSMUSE00001082562"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 5 ENSMUSE00001082562"
     misc_feature    16928..18811
                     /note="Critical Region"
     misc_feature    18835..18868
                     /note="Downstream LoxP"
     misc_feature    18812..18891
                     /note="synthetic loxP region"
     exon            19121..19287
                     /db_xref="ENSEMBL:ENSMUSE00000984690"
                     /rank="8"
                     /note="ENSMUSE00000984690"
     exon            21191..21290
                     /db_xref="ENSEMBL:ENSMUSE00001029754"
                     /rank="9"
                     /note="ENSMUSE00001029754"
     exon            22512..23187
                     /db_xref="ENSEMBL:ENSMUSE00000145636"
                     /rank="10"
                     /note="ENSMUSE00000145636"
     misc_feature    18892..23381
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatcaggg aggttaaata actcaacgtg gctccacata gaaataggaa
     4321 ataaagatat aagtgacggg ctggtgagat ggctcagtgg gtaagagcac ccgactgctc
     4381 ttccgaaggt ccagagttca aatcccagca accacatggt ggctcacaac catctgtaat
     4441 gagatctgac tccctcttct ggtgtgtctg aagacagcta cagtgtactt acatataata
     4501 aataaacaaa tctttaaaaa aaaaagatat aagtgacatc caccttcgta agtgcccccc
     4561 accggcccct ctcccccaac tgtatgccta ccaggtctcc tctaaaactg tcagggcccc
     4621 ttcttctgcc ctgtgtgtgc catttgatca taggaagttg gaaatgtagt tttcttggtt
     4681 agccagacag ggccagcctg gttgtgcatg tgtgtgaggg cagatggtga cttagcccgt
     4741 gggggctttc tggaggagat aggattgctg gtggagctct ggtgagattt gcaccaagac
     4801 atttttcctt ccagcttgct tcccagctgc cctccatgcc ttggaagcct gcccatcctg
     4861 tcaatccagt tcccttcagt ggagtcaggg aagagttggc agaggaaggg cgtgaggttt
     4921 caaagcagct gccctgcgag gacagaatca ccatgccatt aggtgtgggt gtaggagtgt
     4981 ggtccaagga cacttagagt gattggcaga ggcagaggca gaggcagagc aggagccagc
     5041 gggataggag aggagctgta catctggagc cagaatgtcc accaaaggca aacacccacg
     5101 agcagatcag gggtaggtac acctgctgag aggagcctta gaaccagaga gcagggccaa
     5161 tggctgacag aaatcggatg gggaggtcat aggtcagggg aagggccaag agcccagggg
     5221 ttgagaccac taaggagcct agaacttcag agtgataggt ggaccaggag gctcatggca
     5281 gaggtggggc cagggggagt gggtggtggt cttgatcttc cttgttcttc tgagcttcac
     5341 aaccctgtga gaagataggt tcctggaagc acccagcttg ctgggggtgg ggcaagccct
     5401 gtctgtcctg gtggagttcc agatgacatg aggcagcttt gtgggtctat ggggacctgg
     5461 ttgaactgtg accaggttgt taaaaagaaa gggacttctt tggagaccag cccagagctg
     5521 agtttctggt ttcaagttta aaaagccaca gtccagagta gggcagagta gcacaaacct
     5581 gtaatcctag cattgtagaa actgaggcag gaggatcctg gactcaagaa ttcagttatg
     5641 tattaaattc tgtatcaaaa aagcaaacaa agacaggcgt ggtggcgcac gactttaatc
     5701 ccagcactcg ggaggcagag gcaggcagat ttctgagttc gaggccagcc tggtctacaa
     5761 agtgagttcc aggacagcca gggctataca gagaaaccct gtctcgaaaa accaaccaac
     5821 caaacaaaca aacaaccaaa caaacaaaaa tgaggctggg ggtgcatctt agcacagact
     5881 gctagcctag tgtgcaagaa gcagctgctg tgggttgccc tggtgctgtt agtatgtggt
     5941 gttagctctg cttcctcaag aggggttgca gtagatcact caggtgatgt cacagggcct
     6001 tttccccgtg ctgactggtc aaaaaaaggc tacagccaat gactgggcag tggaagggaa
     6061 aggtgggtct ggaggtctca gagggagagg agaaagcagg agggagggag agggagagag
     6121 agagggagag agaggaggtg aaaggaagat ggagcagatc caccgggcct ggcaaagctg
     6181 caaatagctg gggaatagat gagtatagtg atagatcagc ccaatctagg catatagctt
     6241 ataaatataa taactgggtt ctgtggtttc ttatatgggc ttattggggt aaaaaattac
     6301 cgtagcacct agcactgcat aaatggatca tgctgacaca agttagccat tccagaactt
     6361 gggaggaaat ggaggcagga agattgggtt caaggttatc cctggatgca tatcaagttg
     6421 gaggccagcc caagctattc atataagatt atatgtcaag gggagagaga gaggaaagag
     6481 agagggagag agagagagag agagcgcgct ggggttgggg catgttcctg tgtttccaga
     6541 actgtgacat tcttttcttt ttttttttaa acatttattt agtgtgtgtg tgtgtgtgtg
     6601 tgtgtgtgtg tgtgtgtgta gcagcaagaa tgcgcaagtg ctgtggaatg cctgtagagg
     6661 gtggggatca gagcccagcc tgtgtgggtt gttctctcct ttgactgcga tgtggagctt
     6721 caaactcagg tcatcaggtt ttgtggtcac ttactgagac agcttaccga tgggaagtgt
     6781 cttttttttt aagattcatt tattttattt atatgagtac actgtagctg tcttcagata
     6841 caccagaaga gggcaccaga tcccactgca gatggttgtg agccaccata tagttgctgg
     6901 gaattgaact caggacctct ggaagagcag tcagtgctct taaccgctga gccgtctctc
     6961 tagcttgaag tgttacattc ttattgtaga aaatcttgaa gcttctgaaa ggtgttaatg
     7021 ataaatggtg tgatcctagc acataggagc agagacagga ggattgctac aagctcaagg
     7081 ttactagaga ataccaggta gctaaggctg catagtgaga cctatctcta cacacacaca
     7141 cacacacaca cacacacaca cacgcacacg cacacgcgca cgcacacgca cacgcacacg
     7201 agcacatgtc cacacccgtg catgtaaggc tgaagagatg actccgtggg taaagacctt
     7261 gccacacaag tatgaaggcc aaagttcaga tctacactca agaaaaagcc gagtaggcag
     7321 tggtggcgca tgcctttaat cccagcactc gggaggcaga ggcaggcaga tttctgagtt
     7381 caaggccagc ctggtctaca gagtgagttc caggacaccc agggctacac agagaaacca
     7441 tgtccaaaaa aacaaaacaa caacaacaac aacaacaaac ctctaagccc ccacccccca
     7501 aaaaaaaaac caaaaaagga gaaaaaagcc cacacgttaa tccactgctc aaacgtcaga
     7561 caaagggcga ggaccgctga gactgcctaa cgaacccagc caaatccgtg agctgtggca
     7621 gtaggagacc ctccctcagt aaacagagca gaccattgga gaaagcactc agctttaccc
     7681 tgcagccccc acacacgcac ttgtacatac acatgagctc acatacattt ggacacatac
     7741 accatggact gcacataggc acatgaccat gatgttagtg agagaagaga tctgttgggt
     7801 ccttgaggtc tattggccag cagccactgt gagtaggaca aatcagctgg cctcttggag
     7861 ccttggtgtc ctcatcctta caatggggta acagtaactg ccgggcactg ttatcccacg
     7921 caggaagggt aaacgtcgct cttcctccca gccttgttcc ctgttgcatg tggtgtgcac
     7981 agaatccacc ctggctcccc gtgaagcagg tcccatcaag acaactgtcc ctgctacacc
     8041 ccatttctca cactctgcac cccgaagcct ttgccccgct gctcaacatc cacagtgcag
     8101 tgtccctgtg tcttcactgc agttagagcc tgcatgacat gacagctctc tggcctgcct
     8161 ccagagatgc cgatgctgct gatcctgtat aattacagac tcgtgctatg taactgacat
     8221 gtcagtcagt gacagtcagc gtgcacaaca gtaaccctac agattataac agagcggagt
     8281 ggaataggct gccccacatt aaggagcccc agggacctta ctacatccca gcttcagtcc
     8341 cagggaggca gtaggaagga tggacactgt tagaaccaca ggcaatgtga ccaggagctc
     8401 atggagcctg ggcaggggca ctgtgagggc tggtgacatg gtgacatggc accccagaag
     8461 gagctctagg gcatacagtc taggccaatt gttccagttg tccagggact agggaaccaa
     8521 gtagccccag ccaggcccag gtcctccagg gagtccctgc agggaatagc ccctggtctt
     8581 cctggctagg ggtctaggtg gggactcccc tagttctgac tgtaaggaac gagggcttgt
     8641 gtccccatgc aggcctgaca ggaggtgagt ctgctcaggg acagccctgc aggccctgaa
     8701 tgtggaggcc tcagttggga aatcagttgc tctgtctgtc atggggaaga gagcaagtct
     8761 gcactctcct gtctccccac atttctcagc tgggacagcc cctgagagcc tggagtgact
     8821 gttcaattta gcctcttgtt cttaataggc tctgcctaag agttctgtcc acagggggcg
     8881 ctgtcctgag agtcctatca acagggggtc ctgtcctgag agttctgtcc acagagggca
     8941 gcgtcctgag aagatagatt actggagaga tgtgatgttt aagcctggca gatactgtca
     9001 tccattgtat gtgaggatga ggagactgag atgcagagac ccactgtccc tacaggagct
     9061 ctactccaaa gtcttgtagc tgtccactgt ggggcactgt cctgatttaa actctgttca
     9121 cagggagtgc tatttcaaga gctctaatca caagcctctg ctggcattag aggaactcta
     9181 tccatagggg atgctgtaca caagggtgtg tgtctggctt tcagcgaaga aggtgatgga
     9241 ctgctgaagc ccaggggaag atacagatgc tgatgagaac agggcctaaa gggcacacag
     9301 tagggatact attcctctgc tccctccaag ggccatgacc aggtctcagc atgggcagct
     9361 cccgactaca cagatgcaga gctcacacct gtattcactc aggtcaacac aacagctaga
     9421 caagggtaca ggtacacacc cacacccaca catcccccac acccacacac atgcacaccc
     9481 acacacccgc aaacacaggc acgcacgcgc acacgggtgc gcacacacac acacacacac
     9541 acacacttcc acagactcag aatacagatg cttgtttaga cacataaaga cacaaggaca
     9601 gaggcactca tttgcacatg ggcatacaga cagcaacagc tataaaagat gagtcttgca
     9661 ggttggctca tacttttctt ttcagctgtg tgggaagtag agacaggatg atcaggattt
     9721 cccaggtcac ttctaacaat ataacaaaca cttcggaggc cagcacgaac tacgtgagac
     9781 cttgtctcaa aaaaacaacc aaccacacaa ccacacataa cagcaaacat acaaaacaca
     9841 ccaagaacaa gacaagcgct atgtccacca gcgttcagaa gcactcagca ccactgcagt
     9901 gcccaacctg ccaggtcact ggagagaagc ccatggaagg ccacaagctt ctggaggcgg
     9961 cacgggccat gggtgattag agtgtgtctt aatgacggtg tcagtctcaa ccactcagtt
    10021 aattgcccgt atttcaggac ggaccctttt gaagaaaagc tacagaggct gaaggaggcc
    10081 ttcaacacag ggaagacaaa gacggctaag ttcagggctg aacaactgca gagcctgggc
    10141 cgcttcctgc aggacaatag caaacagctt catgacgccc tggatggaga cctgggcaag
    10201 gtgctgtgag ggttggggat ggggcatgca tccttggaca cacagaggtg ctgttctatc
    10261 actcccgggc tagccatatc ctttctgaga tcaggagtcc ctaaggagag tcatacacgg
    10321 caaggtatgt gcccaaaatg tgtgcccaaa ataaggcgca taacgatacc acgatatcaa
    10381 caagtttgta caaaaaagca ggctggcgcc ggaaccgaag ttcctattcc gaagttccta
    10441 ttctctagaa agtataggaa cttcgaaccc tttcccacac caccctccac acttgcccca
    10501 aacactgcca actatgtagg aggaaggggt tgggactaac agaagaaccc gttgtgggga
    10561 agctgttggg agggtcactt tatgttcttg cccaaggtca gttgggtggc ctgcttctga
    10621 tgaggtggtc ccaaggtctg gggtagaagg tgagagggac aggccaccaa ggtcagcccc
    10681 ccccccctat cccataggag ccaggtccct ctcctggaca ggaagactga aggggagatg
    10741 ccagagactc agtgaagcct ggggtaccct attggagtcc ttcaaggaaa caaacttggc
    10801 ctcaccaggc ctcagccttg gctcctcctg ggaactctac tgcccttggg atccccttgt
    10861 agttgtgggt tacataggaa gggggacggg attccccttg actggctagc ctactctttt
    10921 cttcagtctt ctccatctcc tctcacctgt ctctcgaccc tttccctagg atagacttgg
    10981 aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga aagattttgg ctgggcattc
    11041 cttccgctag cttttattgg gatcccctag tttgtgatag gccttttagc tacatctgcc
    11101 aatccatctc attttcacac acacacacac cactttcctt ctggtcagtg ggcacatgtc
    11161 cagcctcaag tttatatcac cacccccaat gcccaacact tgtatggcct tgggcgggtc
    11221 atcccccccc ccacccccag tatctgcaac ctcaagctag cttgggtgcg ttggttgtgg
    11281 ataagtagct agactccagc aaccagtaac ctctgccctt tctcctccat gacaaccagg
    11341 tcccaggtcc cgaaaaccaa agaagaagaa cgcagatctc tagagtcacc gagttgcttt
    11401 accggatgaa gagggccgaa acatactgtc caaggccctt gctggcaatc cacccaactg
    11461 aagccagaca caaacagaaa attgtggcac cggtgaaaca gactttgaat tttgaccttc
    11521 tcaagttggc gggagacgtc gagtccaacc ccggaccggg gatctggact ctagaggatc
    11581 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    11641 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    11701 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
    11761 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
    11821 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
    11881 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
    11941 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
    12001 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
    12061 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
    12121 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
    12181 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
    12241 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
    12301 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
    12361 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
    12421 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
    12481 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
    12541 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
    12601 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
    12661 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
    12721 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
    12781 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
    12841 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
    12901 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
    12961 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
    13021 gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
    13081 aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
    13141 gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
    13201 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
    13261 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
    13321 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
    13381 ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
    13441 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
    13501 ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
    13561 tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
    13621 cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
    13681 ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
    13741 tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
    13801 gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
    13861 tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
    13921 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
    13981 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
    14041 atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
    14101 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
    14161 ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
    14221 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
    14281 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
    14341 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
    14401 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
    14461 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
    14521 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
    14581 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
    14641 gcagggggga tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag
    14701 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    14761 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    14821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    14881 aactcatcaa tgtatcttat catgtctgga tccggaataa cttcgtatag catacattat
    14941 acgaagttat gtttaaacgg cgcgccccgg aattcgcctt ctgcaggagc gtacagaacc
    15001 cagggccctg gcacccgtgc agaccctggc ccaccccacc tgggcgctca gtgcccaaga
    15061 gatgtccaca cctaggatgt cccgcggtgg gtggggggcc cgagagacgg gcaggccggg
    15121 ggcaggcctg gccatgcggg gccgaaccgg gcactgccca gcgtgggcgc gggggccacg
    15181 gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg ccaacgccaa aactctccct
    15241 cctcctcttc ctcaatctcg ctctcgctct tttttttttt cgcaaaagga ggggagaggg
    15301 ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg agcggcgcgg ggccaatcag
    15361 cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg ccgcggcggc gccctataaa
    15421 acccagcggc gcgacgcgcc accaccgccg agaccgcgtc cgccccgcga gcacagagcc
    15481 tcgcctttgc cgatcctcta gagtcgagat ccgccgccac catgattgaa caagatggat
    15541 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    15601 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    15661 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
    15721 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    15781 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    15841 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    15901 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    15961 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    16021 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
    16081 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    16141 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    16201 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    16261 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    16321 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    16381 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    16441 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccc cggatctaag
    16501 ctctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt
    16561 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    16621 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    16681 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    16741 ttatcatgtc tggatccggg ggtaccgcgt cgagaagttc ctattccgaa gttcctattc
    16801 tctagaaagt ataggaactt cgtcgagata acttcgtata gcatacatta tacgaagtta
    16861 tgtcgagata tctagaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag
    16921 taggcggatg gctgttcttg ggtaacaaat agggaaactg tgacacttgg gttaagtcac
    16981 ttacctaaag ttacaagacc aggcagtgac aggtcagaat agcagtcccc atgggagcac
    17041 cgtgcttccc ttacactgtg ttatatacat gctcaaggga actgtcccaa gccctatcca
    17101 aacagtaggc ccagcccagc cttgaacctg acttccttac cccacagtca ggcttcgagt
    17161 cagacatgag tgagatcatt ctgtgcgaga atgaggtgga cctggccctc aagaacctgc
    17221 agacctggat gaaggatgag cctgtgtcca ccaacttggt gagtcctgtt ggctgaggga
    17281 tggtgagtga aggcaaaagt ggggtggcag ggcacggggc agcctgcccc aggcccattc
    17341 ctgcccttga gtccaaagta agccagtctt ctcatgtgga gactccatca cccaggcagt
    17401 ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgttag gcgggctctt
    17461 actcatctgg cacctgacta tggttctgca tcctagctca ccaagctgag cacagctttc
    17521 atccggaaag agccctttgg cctggtgctt atcattgcac cctggaatta tcctgtgaac
    17581 ttgatgatca tacctttagt gggggccatt gctgcaggtg agtggggttc tgagccctcc
    17641 tagtctgatg agtcttccca aagaaggctt ccagaggtcc aagaagtgtg caagcaccag
    17701 cctcccctca ctacttcccc actggctcct ggcaggaaac tgtgtggtgc tgaagccatc
    17761 agaaattagc aagaacacag agaaggtgct ggcagagctg ctaccccagt atctggacca
    17821 ggtgagcatg gctttgcctg tcaagcagct ggcacaccca cacccctgtg ctggccagtg
    17881 gggaggtcct cctgccctga gtctgcctcc tgagctgtct tcctgccttg tagagctgct
    17941 ttgctgtgat gttgggagga cccgaggaga ccgggcagct gctggaacac aaatttgact
    18001 atatcttctt cacaggtaag gagagccctg tccaggacct ggggtagggg gacagcagat
    18061 aatatggtgc aaaagcattg tgggtaaggc cataggccgg ggaggggatg aatcaggagg
    18121 tggaggtgtc cctgagggtg gctctccagg ccctctgaga atcttggaga ttggcttctg
    18181 ggtggtcagg atggaggcag actctaggag gtaggtggaa cagcccagaa cttgggttga
    18241 gaaaggtctc tgtggccagg ggaggccaaa ggtaatgcta gttgaccagg tgcctcttga
    18301 ccatgttctc aggaagtcct cgggtaggca agatcgtcat gactgctgct gccaaacacc
    18361 tgacacccat caccctggag ctggggggta agaacccctg ctatgtggat gacaactgcg
    18421 acccccagac agtggccaac cgtgtggcct ggttccgcta tttcaatgcc ggccagacct
    18481 gtgtggcccc cgattatatc ctgtgtagcc aggagatgca ggagcagttg gtgcctgccc
    18541 tgcagaatgc catcacgcgt ttctatggag ataacccaca gacctccccc aacctgggca
    18601 gaatcatcaa ccagaagcat ttcaagaggc tccagggatt gctgggctgt ggccgtgtgg
    18661 ccatcggtgg ccagagtgat gagggagagc gctacattgg tgagtctcct gtccctgacc
    18721 ctggcacaca gcccacctgg gctgagaccc ttcatactgg aggcaccttt ctggacctaa
    18781 agtctgagtc ctcaactgag ttctagcttt tgagatggcg caacgcaatt aatgataact
    18841 tcgtatagca tacattatac gaagttatgg tctgagctcg ccatcagttc atctcctcac
    18901 tgtgtttctt ggtctgtgac tcctcaattc caaatagctg tcccaggact caaaccctct
    18961 attgtcccat acctcataag gtcaaaattc attaaatctt gagtcctcat agctcagacc
    19021 ctgagattcc ctatcctgga gagcaggctc tccctgtcac agcccgatcc ctctgcaagt
    19081 cctgcctgtg gagaggctga gtctattcct gagtctgcag cccccacagt gttagtggac
    19141 gtgcaggaga cagagcccgt gatgcaggag gagatctttg ggcccatcct gcccctggtg
    19201 actgtgagaa gcctggatga agccatcgag ttcatgaacc agcgggaaaa gccgctagcg
    19261 ctttatgcct attccaacaa cgcagaggtg ggtgcagttt ccagaagtgg gctatggcat
    19321 aggaggcagc agagagcaca gcataatcac agaatttcag gcatggtggc ttcttaggct
    19381 ctgtccttgc cctccctctt aggaggcact gaggtcccct cccatatgct atccagacca
    19441 tactggtttt cctgggcacc acccctggct gaaccaagtg ttcccccact cccaccccac
    19501 ctgccgcttt tttttaaaga tgaggtctta tatagctcag gcctcatacc cacttgtagt
    19561 tgagatagct tcaaactcgt gattctcttg ctttcacact catagtaggg tgataacaca
    19621 ggttcacacc attcctggtt tcaggagcta agaaagctct gccaacaccc acacgccctc
    19681 aacactggtg ttgtaaatac actttttatt tgagaataca tttatggaaa agccacaaag
    19741 gcgatagatt tcctgtgtat cccacactca gttgccccat gacacagggc caggaacctc
    19801 aggaaccagg atgtcttgct ggccacaagc agctctctgg ctcacaggta gcttttacct
    19861 ccagggcagt ctctctggaa aacctatgtc ttttattgaa ttgagaggaa ccaagaaact
    19921 ggggtgttgc aaagaaccag gaaaggacct caaatttcac cactgctggc cctgcacccg
    19981 tccccctccc tctatgcagc ctgtgctttc gatcccactg ggcttcagcc tgtgcagcca
    20041 gatctttgca acacccccgt ttcttggttc ctctcaattc aataaaaggc ataagctttc
    20101 tagaaagata gccctagata taaaggctgc ctctgagtgc cagagagctg ctcatggcta
    20161 gagagattag taatcacttg ggcaaggcct tgtctgtcag cgtctctgct gggagctgtc
    20221 acaccacttc ctgagtgttg gggcaagcat ctagctgacc tctagggagg tgggagatgc
    20281 tacacatctt caaaggatgc taatccttag gctactgcag ttatggtccc aactccaggg
    20341 ggcagaggat ttctcccaga gccatttatc tagacagtgt gtgtccaggg ggcagaggat
    20401 ttctcccaga gccatttatc tagacagtgt gtgtgtgtgt gtatgtgtgt gtgtggtata
    20461 gtttgttttg ttttctgagg caggttcttg tagattaggt tggtgtgatg aattccctat
    20521 gtagctgatg acaaccttta actcctgagg acaatgtcag gctgataagc ccaaacttga
    20581 ccttattggc ccaaactcgc tccataaagg cgttcacttc tctctctctc ctttcttttc
    20641 tttctttctt tctttctttc tttctttctt tctttctttc tttctttctt tcttttttct
    20701 ttttcttttc ttttctttct tttttttttt tttttttttt tttttggttt ttggagacag
    20761 ggtttctctg tgtagccctg gctgtcctgg aactcacttt gtagaccagg ctggccttga
    20821 actcagaaat ctgcctgcct ctgcctccca ctgctgggat taaaggcctg cgctaccacc
    20881 acccagtctc tcttttcttt cttgaagaaa tgtctcagtt ataataagcc tcagcagccc
    20941 gctcatctcc ctcccctggg aaacctccat gaccacctgg tacctgactt actatgcttt
    21001 ccctctgcct gcctctagcc tcaggctatg ttaggactat gtcccaacac cctgctaggg
    21061 ccactcacag gcagacacac agggaacaag ttcccagcgt cagggtccag gaatctatcc
    21121 taccagagcc cccttggccc tcactgaaac aaggtccgtc caggagtgct ttgcatcttt
    21181 tctctcccag gtcatcaaac aggtgttggc ccggaccagc agcgggggct tctgtgggaa
    21241 cgatggcttc atgtacatga ccctgtccag ccttcctttt ggaggagtgg gtaggtagca
    21301 gcagagctct gccctcctgg gctgaccccc tacctgcatc cccatcctgc tacctgacca
    21361 cattaactgc agaagatgtg cccccttctc agtgcccagt gcagcaggtg ggagcataag
    21421 gccctcccca ctgactgtcc aactagcctg cctctctccc acctccctac agtctgtggt
    21481 ggaaatcttg tgtgggtgag gaccaaagag gttgttttgt gcattttaag aaagcccacc
    21541 tgtagctgcc agccactgag catcctgact ttcatcaagc aacacttgtc ctctgctgcc
    21601 catcccccac atggctgtgg gtgttttcta atgacggggc tgcatacttg cctcttcagc
    21661 cttcccatgt ccccgtgaac ataggcctgg ggacttccac ttccaggatg cctggaatgt
    21721 tgaaatgcaa gccccacaga cagaggtgac cttggcaacc actttccctc tgagcccacc
    21781 tcattactga aaggctatcc tgttccctcc acccggtaac tttcagtggt ggaatgagtg
    21841 acagatgagc ctggtccctc ttggtctgtc tagaggacct gctcccatct ctctccctca
    21901 gttttgtctg ctgtggcagc cactgagctt ggtcctaaca tgtcaccgac ggccaaggct
    21961 cttgtcccca ggactgattc ctttgggctt tcattcacaa cattcatctg gctgactcca
    22021 caaccctctg gcctctttca ccccagaaac cccagcttat ggccccctac tttacccaaa
    22081 ccattcccca gaactctcct ggctggatcc ttgtcccttc ctagacagtg ggaactctgt
    22141 tgagtctgaa gattcactta atttgtagca cagccctaga aggaggatgc caccatcaca
    22201 gaagacagag gcaaagtcac ttactcaagg tggcactgct attcaggaca gagctgggat
    22261 ccgtccccca cccccacctc agcagcctgg gttcagaata taacccatca gtcactgtct
    22321 gcttactctg caccaacacc tggttagctg ctgctggggc tggctcaggg ctcagcattt
    22381 tgcacacagt tctttattta ccccactgag gaaacagagg ctcagatagc ctccctgtca
    22441 gagctaggtc tgtgcgatat cgtctcaact gtccactgaa ggcctaaccc tatgtcctat
    22501 gctgcccaca ggatcgagtg ggatgggcag gtaccacggc aagttctcct ttgacacctt
    22561 ctccaaccag cgtgcctgtc tgctgagctg ccctgggatg gagaagctta atggcctccg
    22621 ttacccgccc tatagtcccc gtaggcagca gctgctaaga tgggctatcg gctctgagag
    22681 ctgcaccctt ctgtaaacac cacaccgact cctctctcct gctgccctgc cccaacctat
    22741 ttaagctttc atctgcttcc aagtccccag tggttcctga aaggcgagtg cctagtccag
    22801 cctgtgaaca ccccatttct ggaatttttt ctgagctacc tgtagccagg catgacatgt
    22861 agatggctgg tccagcccct tctcatagca tagcaccctc agcagccttc caaccttctg
    22921 cttagacaga gaagccccct caataagttt gggcagatct agtgactaag ggggacttac
    22981 tttgtcagat cgtaacagga tcccaaaaga cagagcagtt gctcacaact ccatccacac
    23041 ctggagggtg gctttgtccc acctgaagtg acatgaccat ggacccagat cagattattt
    23101 ggtggaatct gcctgtctga agtgtgttca ttagttgctt tcctgtgact tctattaaat
    23161 gtcctgatac ggacaagttc aagagggaag gattctcttg cagctcatgg tcagaggatt
    23221 cagtcagctt caacatctga tgaagttaag gatcttgcca agatcctggc aatccattca
    23281 atgctcactt cagtcacagg tgtcatcctc agagagaagc agagggtgag gatgtagttc
    23341 agttgtattt tgtcaagtgt gtgtgaggcc aggagaaagg a
//