LOCUS       KO-first_condition_ready_98839_MGI:1354709        23027 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Sap30l targeting project(s): 98839
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_GT0_LF2A_LacZ_BetactP_neo
COMMENT     design_id: 462084
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000020826
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7955..8077
                     /db_xref="ENSEMBL:ENSMUSE00000105597"
                     /rank="2"
                     /note="ENSMUSE00000105597"
     misc_feature    4275..9604
                     /note="5 arm"
     misc_feature    9632..9656
                     /note="B1 Gateway"
     exon            10592..10626
                     /note="mouse En2 exon"
     misc_feature    9669..9716
                     /note="Frt"
     misc_feature    10589..10591
                     /note="Splice Acceptor"
     intron          9717..10591
                     /note="mouse En2 intron"
     misc_feature    complement(9785..9806)
                     /note="LRPCR LAR3_EN2"
     misc_feature    10492..10513
                     /note="seq primer FCHK2"
     misc_feature    complement(9785..9806)
                     /note="seq primer L1"
     misc_feature    complement(10915..10934)
                     /note="seq primer FCHK1"
     misc_feature    complement(9681..9704)
                     /note="FRT3"
     misc_feature    13695..13715
                     /note="seqlaczF"
     misc_feature    13910..14165
                     /note="SV40 polyadenylation site"
     misc_feature    13980..14003
                     /note="RAF5"
     promoter        14228..14748
                     /note="human beta actin promoter"
     misc_feature    14169..14202
                     /note="loxP"
     misc_feature    14624..14643
                     /note="betact5"
     gene            14774..15759
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15766..16009
                     /note="SV40 polyadenylation site"
     misc_feature    16026..16073
                     /note="Frt"
     misc_feature    16080..16113
                     /note="loxP"
     misc_feature    15824..15847
                     /note="RAF5"
     misc_feature    16006..16026
                     /note="PNF seq primer"
     misc_feature    10627..10810
                     /note="LF2A"
     gene            10811..13882
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    10606..10631
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10606..10621
                     /note="FCHK QC prefix region"
     misc_feature    10622..10625
                     /note="FCHK QC critical region"
     misc_feature    10626..10631
                     /note="FCHK QC suffix region"
     misc_feature    16129..16149
                     /note="B2 Gateway"
     misc_feature    9605..16179
                     /note="Synthetic Cassette"
     exon            16484..16582
                     /db_xref="ENSEMBL:ENSMUSE00000105598"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000105598"
     exon            18402..18677
                     /db_xref="ENSEMBL:ENSMUSE00000335155"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00000335155"
     misc_feature    16180..18747
                     /note="Critical Region"
     misc_feature    18771..18804
                     /note="Downstream LoxP"
     misc_feature    18748..18827
                     /note="synthetic loxP region"
     misc_feature    18828..23027
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagtctg tgcaaaacag gggactcagg ttcttggagg ccgctcggtg
     4321 ctccgcgacg ctgcccgcgt ccccgagggt gggctgggaa accagggagg gcttggcaca
     4381 ctctccctgc ctgggaaagc gacagcagtt tccagctgtc actgttgcgc cgaatagcag
     4441 agcagagaga ggtgggttac ttttgcaaga attgggcaaa agaggtgaca agggaaccgg
     4501 gaggatcttc tccaacttcg tcctccctcc agggaccttg tcgctctttc agacactagc
     4561 aaggtggccg cgactttcct gtctgccgcg gcctccggtc tctgcagcgc aacacagagc
     4621 ccgcttcgct ggcagcgaat gagtttttct gagatgtggc agaaataagg ccaaggccgg
     4681 gtcttaaatg atttgctgtg gatgtgattg aagggggcgg ggaatcagct ttcttgcggg
     4741 gattagcatc tttggtaatg ccgttggagg tggagaggtg caaatttaaa atggaatgag
     4801 attgccctat gccttgtggc taattataag aaacttcctg agtgatttca cctactctgg
     4861 tcctcaagat gtgcctactg ccaaccggtt aagaactttt cagtttgaca tctgttgctg
     4921 caagccctga gtggcagctt ccaggttagg aattgagact gagttaagga acttctcata
     4981 ttcaggagtt tgaatgatta ctgttcaacg gtttggggaa atcagacatt gcttggtagc
     5041 gggaaaggga gccgatcttt acacttctga tgtggcagaa actgaggctg ggggcggggg
     5101 ggtaggaagc aatggcgcca agagcacact gcacttaaaa gctggctaat ttcctaagcc
     5161 cacttattgc ctggcttgct tccaaacctt acacccagtt ttcattcgtc tataataaat
     5221 aagatcatca tcctttcttc tagactcttc tcttgcacac tcagttttat ttagtcaacc
     5281 agtagaatcc agctcatttg aaatcgtgct gggttttgtt ttttgttttt ttatgattgg
     5341 gaccttcctt tttacaaatg tcttgtcagt aaataaatga gtgagtgaat gaatgaatga
     5401 atgagattac tctgaagcaa agctggctaa accctcaggt gtcccctgct aatcacacat
     5461 gggagtgaaa gtgatcccga gactggggat gagtttatcc acatggaaac ggtgcctgcc
     5521 tcccgtgtgg gctgctggca gtgctcacat ctggatggtt ttgttgttgc agtaaagact
     5581 ccatttatcc actcacccca tggggaagcc atgtggagca gtgttgagca tgtcgatgtc
     5641 acacacactc acacacacat catcatcatc tggaagaaag gccccctctg ccctgtacta
     5701 gcatccccaa aggcagtact tgggaaggct tgtggttaga tgtgcggcct tagtccattt
     5761 caaggtggta gtacagagtc attatctctg aaacagtttc tatttcattc cacagatggg
     5821 caggaattca gtcccaagta agtgctgtgt tactagaatg tctagctgtt gtagcaagtg
     5881 aaacaggaaa gaaaaaacaa acaaacaaac aaacaaaaaa acccagacaa ccacagtgag
     5941 cctttgcttc caccctaagg cccccacctc acccacaaaa gcccctgagt cctgagattg
     6001 ctgaaaagca gattagactt gaacatggtt aaggacagag aatagctgtc tggtaaaagg
     6061 atctggcaaa agccctttta aaatactata gcacccccct tcaggtggcc actggctgcc
     6121 tctgtactgt attagtacat agccaacttg ccgctgatgg gtttcttctt tgcttctgga
     6181 gagtgctcga agctggggat cacataaagg aaacaggctc cagtgtcgct tgggttgctg
     6241 ccccctgctc tggtctggcc tcttcctacc taagctttag aaacacccca ctctgccact
     6301 cattcttccc agtttaccac tatgctgtgg gaagccactt gcgctctctc tctctctctc
     6361 tctctctctc tctctctctc tctctctctc tcttggtttc tcgagacagg gtctctctgt
     6421 agccctgact gtcctggaac tcactctgta gaccaggctg gcctcgaact cagaaatcct
     6481 cctgcctctg cctcccaagc gctgggatta aaggcgtgcg ccaccactgc ccagcaccac
     6541 ttgctctctc aagagccact tacttacatt gtcagcccaa gtgccagctg ccaggaatgg
     6601 agcagaggtc acatgtcctc ttgtttcacc tgggtgttac tctttttggt tttttcgaga
     6661 cagggtttct ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct
     6721 cgaacttaga aatcctcctg cctccccagt actgggattg aaggcgtgtg ccacctctta
     6781 caagttcaca aatttcaatg acttttgtca gattccactc tggcaatgaa cttttggaca
     6841 ttgtccatag gccactgtcc taaagagtaa aacccagcca ggcagtgatg gcccacgcct
     6901 ttaatcccag cacttgggag gctgaggcag gcggatttct gagtttcgag gccagcctgt
     6961 tctacagagt gagttccagg acagccaggg ctacacagag aaaccctgac tcaaaaaata
     7021 aaccaaaaaa aaaaaaaaaa aaaaagaggt ggcttttacc agcaaaagtc atttggccgt
     7081 ttgtagatat gagagggcct acattggtgc atcagcactt atttctcgcc tgctgtgtac
     7141 aagacagcat cacagtgtaa actcggaatg actcatgact caccaatcca accacaagaa
     7201 actgataatc atcaaattaa acagttttca ttgttccctc cacaaagcag cctgaattca
     7261 ttgatggtga cttcctgcca gcttactttt atctttaagg atttaaggac caaactccag
     7321 ttcagatgtc tccctagaac atttgtgcac catctgtccc tacccaggcc aattacacag
     7381 gccctgggtc ctgcagggtc tttgagtggt tcctccaggc aactgtgcta cctacattct
     7441 taaatgaaac cctgttcttc actgctgagc tgcttttggc gcctttacta atatactctg
     7501 gtgtttatta gaaaatcaga gtgatgtcca ctctctttta tgtggtcccc ctttgtgagg
     7561 gttctgtctc tctctgctcc tttgtggatc agatgtgaag cagcaggagt caagttttcc
     7621 ggtcttaccc acagtaggag taggcgctgt gagtaccatc cactgtcctg cgacctgtct
     7681 ctgcccattg caagttcata attcctcctg tacctgtaag gctcgtttgc aggggctggg
     7741 ggggggggga tatccaagtt gaacatgtac tttccactga gctgtactgc cagccctcta
     7801 tgtttcttct tctgcagcct atatagaatg gttgtaagct gttgtctgcc tttcactctt
     7861 ccatctctct ctagtacctg gccaaaagag aaggctcatg atctcacagg ttccatgggg
     7921 ttggtaacac tgactttagg tttcttccaa tcaggtaagg cacctgtata tctgcgactt
     7981 ccacaaaaat ttcatccaga gcgtccgaaa taaaaggaag aggaaggcaa gtgacgatgg
     8041 cggagactcc cccgagcacg atgctgacat ccctgaggta aaggccagag tgctggtaga
     8101 gagagcaggg agaatagata gtgagtggcc caagcctccc tccctccttc cctcccagcc
     8161 agagaatgag aggagtgggt ccagactcca gctttctccc tggctattgt gtgttcagaa
     8221 ctagtcagtc atctttgtct gcttgccttg ctgcggtgtg tttgaagaga gttctctaga
     8281 ctttcagtaa cactgactgt gaaagcttgg tgatgagctg ctctgcctgc ttgcctggca
     8341 ctctccagac aggcccgagg ctccgaggtc ccaggtggta ctacaggctc cgctcgcctt
     8401 ctttgtagta gactgcaggg ctttgccttt gagcccaacc ggtgcttaac atgatgttgc
     8461 ctcttttaac accctgatct tgcctttaaa gttaatgttc agtctgtgat ctagacagct
     8521 cttgctgcct tctccgacct gaatgtaaag gttcatcttg tcagtgtcac cagctgggct
     8581 aaggcttgtc cccagagaca cactgcagcc atttagccag tgctgaaatt tgacatttga
     8641 ggggaggggc acatcatgag aatgatagga tcagcagcac acacacacac acacacacac
     8701 acacacacac acacacacac acaccttgct agaggccagt gacattgttg tgacagtgca
     8761 aacatgttct gcacacattg ccacatatcc tttgggatgt gcccccaaat taaagagcac
     8821 tgtataagac catgcatccc agcttgtcgc ccagaaggtt ccatgtgtac taagggagct
     8881 ttcaaggtta tcggcgttcc cttccaaagt gttcccactt gggctttgtg acgaggaact
     8941 gtgcccagtg tgcactgagc atttgctgcg tcacacttca cggcttcgag tagaaggagg
     9001 taggccttgg tctgcgggtg tggagaggcc tgggaagctc agccgtgtcc ccacacctct
     9061 ccctatggcc ctccccagcc ttactttctg atatcttttg cctgtagcat tgctgtcatt
     9121 tcataaagac tgaatccctt tgaccaagca ttttagaaaa gttttctaag aatgttttga
     9181 agtgaatgga ggattctgga ccctcatatt ggtcgttttt tttttttttt tttttttttt
     9241 tccttcaaat gaattccact tgcctgaatc aagagcctct cggaggctgg tagaggcaac
     9301 caaaggatct ggcccttctg tatgtcatga gtaggtagct taccctcttc ctaggttagc
     9361 tgaacaagag acaggcgccc ttcttgtccc ttatccagct ttctgcagag acagcagccc
     9421 tgacctgcag gaggatcact gtgcagcctt tcaggaaggt gacctctgag gctgagcacc
     9481 cctgctcctc cactgctgtc ctgtgagctc tgcccagcca cacgctagct tatctccttc
     9541 aggggtccct cagctggcct tcctggatac actggtgtcc tttagcactg cccgcagtct
     9601 gctcaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg
     9661 ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac
     9721 cctttcccac accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg
     9781 gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct
     9841 tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa
     9901 ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc
     9961 ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc
    10021 ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc
    10081 tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg
    10141 ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct
    10201 gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca
    10261 aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct
    10321 agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac
    10381 accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca
    10441 atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca
    10501 acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta
    10561 acctctgccc tttctcctcc atgacaacca ggtcccaggt cccgaaaacc aaagaagaag
    10621 aacgcagatc tctagagtca ccgagttgct ttaccggatg aagagggccg aaacatactg
    10681 tccaaggccc ttgctggcaa tccacccaac tgaagccaga cacaaacaga aaattgtggc
    10741 accggtgaaa cagactttga attttgacct tctcaagttg gcgggagacg tcgagtccaa
    10801 ccccggaccg gggatctgga ctctagagga tcccgtcgtt ttacaacgtc gtgactggga
    10861 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    10921 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    10981 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
    11041 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
    11101 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
    11161 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
    11221 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
    11281 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
    11341 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct
    11401 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
    11461 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
    11521 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
    11581 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
    11641 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
    11701 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
    11761 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
    11821 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
    11881 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
    11941 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
    12001 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
    12061 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
    12121 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
    12181 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
    12241 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
    12301 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
    12361 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
    12421 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
    12481 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
    12541 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
    12601 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
    12661 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
    12721 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
    12781 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
    12841 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
    12901 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
    12961 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
    13021 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
    13081 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
    13141 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
    13201 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
    13261 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
    13321 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
    13381 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
    13441 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
    13501 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
    13561 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
    13621 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
    13681 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
    13741 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
    13801 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
    13861 gttggtctgg tgtcaaaaat aataataacc gggcaggggg gatctaagct ctagataagt
    13921 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    13981 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    14041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    14101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    14161 gatccggaat aacttcgtat agcatacatt atacgaagtt atgtttaaac ggcgcgcccc
    14221 ggaattcgcc ttctgcagga gcgtacagaa cccagggccc tggcacccgt gcagaccctg
    14281 gcccacccca cctgggcgct cagtgcccaa gagatgtcca cacctaggat gtcccgcggt
    14341 gggtgggggg cccgagagac gggcaggccg ggggcaggcc tggccatgcg gggccgaacc
    14401 gggcactgcc cagcgtgggc gcgggggcca cggcgcgcgc ccccagcccc cgggcccagc
    14461 accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct cgctctcgct
    14521 cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac tgtgcggcga
    14581 agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag ttgcctttta
    14641 tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg ccaccaccgc
    14701 cgagaccgcg tccgccccgc gagcacagag cctcgccttt gccgatcctc tagagtcgag
    14761 atccgccgcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    14821 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    14881 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    14941 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    15001 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    15061 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    15121 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    15181 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    15241 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    15301 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    15361 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    15421 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    15481 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    15541 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    15601 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    15661 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    15721 ctggagttct tcgcccaccc cccggatcta agctctagat aagtaatgat cataatcagc
    15781 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    15841 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    15901 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    15961 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ggggtaccgc
    16021 gtcgagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgtcgaga
    16081 taacttcgta tagcatacat tatacgaagt tatgtcgaga tatctagacc cagctttctt
    16141 gtacaaagtg gttgatatct ctatagtcgc agtaggcggc attccctcca gctccaccct
    16201 gaagcacccc acacctgctg ccccagccca ctcagaatca tccccctctc tcctcctcct
    16261 cttcttctac ctcctcctcc tcctcacttc ctctccacta ctgcctctct tctgcctcaa
    16321 cctccctcct ccatacacac agagccctct ccacacccgc ctttggcttc cttgccagtc
    16381 cctagtcctt ggtggacagt gtctctgact tcttccctcc tcagccctcc ctagggcatg
    16441 ccaggcaggg tgggctgaga cataactctc tcctctttcc caggttgatc tgttccagct
    16501 gcaggtgaac acgctccgac gttacaaacg gcactataag ctgcagacca gaccaggctt
    16561 caacaaggcc cagctagcag aggtaggtgg taaagcagag aggcccggcc tacactcctg
    16621 agtcccagct actcctccca caggtcatca agagctctgc acatgggcag agaaagccct
    16681 agttcccaaa ggaagccatg cacagagagg gatggacttt ctttaaaagt cacgtggcag
    16741 cttgaggact cctggttctg agtcgtgaca tcatttttgc aaggttcttg atatgtcctt
    16801 acaggtgtgt cagtgtgatt gtctgctgtg tctttaggtt tagggcttga gttctctttg
    16861 ggtaatgaag aacaagccca ggattctttt ttttttttta agatttattt attattatat
    16921 ataagtacac tgtagctgtc ttcagacacg ccagaagagg gcgtcaggtc tcattatcag
    16981 tggttgtgag ccaccatgtg gttgctggta tttgaactca ggaccttcgg aagagcagtc
    17041 agtgctctta cccgttgagc catctcacca gcccaagccc aggattcttt atatgacaga
    17101 cctactaatg agctttccct tcaacctcca agttcctccc cccctccccc ccccacacac
    17161 actttcttga cctctgatat taatggaagc cttttcttat ataattagtg gcacccaaga
    17221 acactttttc tttgttgagt ttttaactta cttttctgtc tgtgatggtg agtcactgtt
    17281 agcaactctt cccttccctc tcttgtgtgt gatccagcag tgctgctggg ttcaactgat
    17341 cctcctgcct cagcttacct tctagatacg cgacacacac ttggcttcct catccctctt
    17401 gattcaccta ccttttgttg taaaaaaaaa aaaattgaga ggcagagaaa gaaaaacata
    17461 atccagagat ttattatttc caggtctttt cttctgccct gaccacctcc atgcatgcag
    17521 gtttactgaa aagctgctgc ccgctggctc cctttgggct ctggctgatg tgtctccact
    17581 cttcagcctc tccctcacct gcgagagaga ggtacaacag cagcgcacag cattgctgct
    17641 taccattgcc agctacaaag aagagtaggg aagaggaggc agtgtgtttg tccaaggcta
    17701 agacagcaac agcagagtcc ggccatgagc agggagcgcc ctcctttgta ctggcagtct
    17761 ttcccaggag agtctgtccc cacttagagg aattcttggg gccagacaca tctgtttgtt
    17821 catttgacat tttggggtgg tttgttttgt tgcgcgcgca cgtgcgtgtg cgtgtgtgtg
    17881 tgtgtgtgtg tgtgtgtgcg cgtgtgtgtg tgtgcgtgtg cgtgtgcgtg tgtgttgtca
    17941 aggcactgag gctggtcttt ggactcctga cctgtctggc ctcttgagca ctgggatcac
    18001 aggcattcac caccacaccc agcttgacat aatttttaaa gccttttttt tttggtctac
    18061 ttccagagcc tgaaaggctt ggtggcaatt ggccaactga ggaagtctca gcctccttac
    18121 atcacagggt agctgaagtc atgccaggga tggctagatg ctactgaagt acatccatac
    18181 cttggtgccg tcctgggcca tagtggtgta cttctggtag cctgggaaaa aaacagctaa
    18241 gctacggagc aaggggcccg ctacacccta cggctccttg gcaagtctga gtgccaggcc
    18301 atcgctgctg gaagtagctt ctcttccctc tttgcagcgt gtaagatgtg gggccctatt
    18361 tgcacatagc ttaagggact ttggtctttt ctcctgtgta gactgtgagc cgacacttca
    18421 ggaacatacc agtgaatgag aaagagacgc ttgcctattt catctacatg gtgaagagta
    18481 acaggagcag actggaccag aagtcagagg gcagcaagca gcttgagtga gctacagcca
    18541 cctgcccgtg tgaggtgggg gccgtgatga aaggacagcc gggtactgct tgatgacagg
    18601 tggcctctgc aacatctcag ccaataagga ctgttcatat ctactgtaaa taaagtttga
    18661 atgatggata ctgtaaatgt ctcggtcagg aggattatat tctcacaggt tagcatgtgt
    18721 gtaaaaagac tttaagatat ctgctcagag atggcgcaac gcaattaatg ataacttcgt
    18781 atagcataca ttatacgaag ttatggtctg agctcgccat cagttcaaac cagtgttata
    18841 aaaggagttt taaaaccgtg tctagagtgc tcagcaaggc tttaaatcag ttctcctaga
    18901 aatgctgggt gatacgcatc ttggccatca ccatgcagtc ccaggctgtc acaagcaagg
    18961 cccagccctg gcagcgtggc tcggtgacag tggaattaca gagtaggccc atgtttgcgc
    19021 tgacccttgc ccactgggaa cagagggatg tcactttatt gcaagttttg ccatttagta
    19081 gcaaatcagt tggacactag ctcccacctt gccctgtgac ctgtacacag tggacgctca
    19141 gagtgtgctg gccaagagct gtcgcagcta cgcagcttct gcttcacccc tcccaccact
    19201 gagactgggg ctttgtgttc tctctgctta accactagag gtggaggaca ggctgcccct
    19261 ccacaagaag tccaggcaag tgaggatctc ggcacagttg cacctgcaaa actgttgaga
    19321 ttccagcatc aacacaaaga catcacaggc tggccatgat ggtgcatgct ttagtcccac
    19381 cattccagag aatgagacag gatagtgagc tcaagggtaa cctgggctaa agaccctatc
    19441 tcagaaaaca gtgaacctca ggatggctca gtaggcaaag acacttgctg ccatgcctga
    19501 tgacctgagt tcagttcctg gccatgtagc agactgtccc ctgacagaca cacacacaca
    19561 cacacaaaat aagtgattta ttgtttttaa actataaaca aaaccatagc cttccatagc
    19621 agtctttgca ttttacccag agtaaggtgt atagcatgac ctgcatgccc ctggcagtga
    19681 gcgctctgat ggcagggatg gggcgtccgg gctcgggttt ctggtcttta gtacacagtc
    19741 tgcaggagag ctgccaagtc cctgtgatcc agctccgaag cccagctctg gaaagacagt
    19801 gtggctgggc tcggctcctt ggtcctagga ggcaggagat catgaggcta ggcaagaaaa
    19861 gccactgcca cctcaagttc aggggaaaaa atcagaatcg gcctcctttg gggagaatgc
    19921 ttactaaggt aattttttca gtattaaatt ttgtcgttgt tcaaatcaga cccttctatc
    19981 tagttctctc aaaaccagaa gcaccctgga aagcagagct ctcttgtaca aagaattgta
    20041 tagaatttcc agtcagtttg tggggtaaaa ggtgtgtgta ggaggataag aaagattcct
    20101 aatttccaac tatcctgtat gtattgggcc aaatgtctca tctccctatt ccacttcaat
    20161 tctgagtttt tcttaagtcc taacagaaca gaggcctggt ctgacagttt taatgtctcc
    20221 caggtggctt gcctgagcgc catctcagag cagaactaac cccacactga gtgagtggag
    20281 gaatgtgtga tggtttccct ctatggagca gagcgtcacg cttctgtgga gtgggaacag
    20341 ggcccttcgt gttactaaca tgcagtgttc cattacggct gagctcccaa gaacactcaa
    20401 aatgcagtcc tgagcctccg tgcagaagtt ggtctccctc ctaccacgga tggaaacaac
    20461 aggagagccc aggcgaagct ggtgtctgag ggcaaatgga atctttaact taaccggccc
    20521 tgtggaaagt gtcttatcaa gaagaaaatg tagctttcta atactttaac caaaactgtt
    20581 agcatgtaag ccatgtgatt gtttctttaa aatctagttt cctgtgaaga ggctcacttc
    20641 tgggcaagat gtggcattag gctccctcta gtccacgttc taagtccaga tccaagctgg
    20701 ggagtgtctg ccctccagcc agatcccacc tcagaatcct tgtcctctga tgccagcagc
    20761 cttggagaag tagagacctg ggctatcatt ttcacccttt cacaacagat cgcctgagag
    20821 aaaaggcaaa gctatttgaa catgctgtgt catgaagtgt gctgtgtcat tggagcagca
    20881 gtccctggcc tcccatctcc ctgcctagtc atggccaaga acccactgcc ttcaagaaag
    20941 gcccaagcct ggcttaggga gcagcaggga ttattgagag agtagtgctt atctaggctg
    21001 cttgtatttt catatcaccg gccatgcctg cctccctcac acatgcatgc gcgtgcgcac
    21061 acacgcgcgc acacacacac actcatctgt gatggctcag catggtctct ctacttggtg
    21121 cttcttgaca gaactggaat ttcaagccca gaagtggtat ggcttcctgt ctctgatcca
    21181 gcctggtgtg gttcttcaat acaccctgaa ggggtcacat ggagacaccc ttagcctgcc
    21241 attcatttgg ggcttgaaaa tttttacaac acaaatgatc agaattctct catctcatgg
    21301 aactaactga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga
    21361 gagagagaga gagagagaaa tggtaaacca aaattccagg tggggattgt gactctgtga
    21421 aggaacttgg cttctgtgtc ctgcatgttt ctgccataag tagctatact cataatagcc
    21481 tctcatagag caaagcttta atgtgcatgc gtgtgtttgt gtgtgtgtgt gtttgcacac
    21541 ttcctgttaa tattttacca agtgactgcc tggtttgtgg tggcaggata gcttgtccac
    21601 tgcagcaggt ggtccctggc atagtcccaa agaagttgcc tcattgatac catctgaaag
    21661 ctctctgtgg tccaagggat ccaaaggttg caaagagtct tgagtcctcc cgtccagctt
    21721 ctccattggt gctgacattc tgcctatacc catcccagcc aagaagcagt ctgcacctgg
    21781 tcacctccaa ggacagagag ctcactacct cctagggaag actgttctgt tgttagcttc
    21841 tggcttttcc aggctctcta agttgggggg ccatgtgggc agagtctctc aatgggcaaa
    21901 ggacccagtc ttagcattgt ctcacacatt gtatcctttt tgcaaaaggg aacagctgga
    21961 tacaatgtgt tttcacgccc cctgacccct cagtatgggt ctattggtct tggttccatg
    22021 ttggaagtgt tggagaaatc cctcagccta actcatgcca aagttgccag gggggggcac
    22081 cagcctgcta acaagagtcc acattaatgg aggttgtagt cccatgctgg ccacacaaaa
    22141 tctgtggcat ctctgagggg taacattagc tggagggttc taactaaaca gcatagggct
    22201 ttgcctggag tttaaggtcc ctggcttgcc gagggctcgt ctcttctggt tcccatgtgg
    22261 acgcagtcac tctgaaagct gaaggtactg ttccacatag tcacttaagg ttgctatccc
    22321 attgtctttc taagtgaatg agggaaagac tttccccaga gatctgtcaa ggtcatgtta
    22381 gggaggggcc tttatggcct tcatttgcac actgtagact tttgcttgtg acaggtgacc
    22441 taaaataagc tgctctggaa agtctcagag cttatccctg ggagcttctc aatggcagtt
    22501 gggctccagg gaccaataga cctgcagaat tgcaggagag tggggattgc tcagcttagc
    22561 aagatgactg gccaaggagc agaccagctg tatttaaaac ccagcaggca tgcccaccac
    22621 agctcccagt cattcaagat agcaatgaat gtgacccaac agagagtcac aattacttag
    22681 agcattatga gacttacctt tcaatttttg aaactcagtt gtgtcattct tgagcatgag
    22741 ttttgttgat aaccattagg cccagacaag tcaagattgg accaacaccg gtggggtgta
    22801 atgttagcct gccttctcta gccttataaa gtgggttcta tctctgccca actcctgcct
    22861 tccatcctgt tgctgaaact ggaggatggc cctggctcat gggaagtttg gtgcctttgg
    22921 ggtgcctttc cactgtttga ttggatcaca gactgcttcc agtttatggt tggtaaaaga
    22981 aaagtcactg tgatgtttaa ggtgggcaaa gtgggcctgg ggcttaa
//