LOCUS       KO-first_condition_ready_126085_MGI:107567        22214 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Irgm1 targeting project(s): 126085
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo
COMMENT     design_id: 470641
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000049519
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7883..8021
                     /db_xref="ENSEMBL:ENSMUSE00000386056"
                     /rank="3"
                     /note="ENSMUSE00000386056 5' fragment"
     exon            6106..6324
                     /db_xref="ENSEMBL:ENSMUSE00001014949"
                     /rank="2"
                     /note="ENSMUSE00001014949"
     misc_feature    4275..8021
                     /note="5 arm"
     misc_feature    8022..8042
                     /note="oligo append B1"
     intron          8022..8168
                     /note="5' Ifitm2 intron"
     misc_feature    8169..8193
                     /note="B1 Gateway"
     exon            9129..9162
                     /note="mouse En2 exon"
     misc_feature    8206..8253
                     /note="Frt"
     misc_feature    9126..9128
                     /note="Splice Acceptor"
     intron          8254..9128
                     /note="mouse En2 intron"
     misc_feature    complement(8322..8343)
                     /note="LRPCR LAR3_EN2"
     misc_feature    9029..9050
                     /note="seq primer FCHK2"
     misc_feature    complement(8322..8343)
                     /note="seq primer L1"
     misc_feature    complement(9466..9485)
                     /note="seq primer FCHK1"
     misc_feature    complement(8218..8241)
                     /note="FRT3"
     misc_feature    12461..12716
                     /note="SV40 polyadenylation site"
     misc_feature    12531..12554
                     /note="RAF5"
     promoter        12779..13299
                     /note="human beta actin promoter"
     misc_feature    12720..12753
                     /note="loxP"
     misc_feature    13175..13194
                     /note="betact5"
     gene            13325..14310
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    13831..13849
                     /note="neoSphI_5"
     misc_feature    14317..14560
                     /note="SV40 polyadenylation site"
     misc_feature    14577..14624
                     /note="Frt"
     misc_feature    14631..14664
                     /note="loxP"
     misc_feature    14375..14398
                     /note="RAF5"
     misc_feature    14557..14577
                     /note="PNF seq primer"
     misc_feature    9178..9361
                     /note="LF2A"
     gene            9362..12433
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    9143..9182
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9143..9158
                     /note="FCHK QC prefix region"
     misc_feature    9159..9176
                     /note="FCHK QC critical region"
     misc_feature    9177..9182
                     /note="FCHK QC suffix region"
     misc_feature    14680..14700
                     /note="B2 Gateway"
     misc_feature    14990..15010
                     /note="oligo append B2"
     intron          14701..15010
                     /note="3' Ifitm2 intron"
     misc_feature    8022..15010
                     /note="Synthetic Cassette"
     exon            15011..16125
                     /db_xref="ENSEMBL:ENSMUSE00000386056"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000386056 central fragment"
     misc_feature    15011..16125
                     /note="Critical Region"
     misc_feature    16149..16182
                     /note="Downstream LoxP"
     misc_feature    16126..16205
                     /note="synthetic loxP region"
     exon            16206..16611
                     /db_xref="ENSEMBL:ENSMUSE00000386056"
                     /rank="3"
                     /note="ENSMUSE00000386056 3' fragment"
     misc_feature    16206..22214
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaggtat catagtgacc tttttgtctg actccattaa aatgttttat
     4321 ttgcattgga gaaagtgtct ctgtttaagg aaccacctcc ttcactagtc tgtaagttta
     4381 tagttaatga gatgggagct tttcaaatac catcttccca gagcatgtgg gttcagtact
     4441 gtccccattc accgtgagca agtttatcac actttccaaa gataaataaa tatcagaggg
     4501 aattgtgtgg tccctgctac ctttcatgca gctatgggca agtgtgtgcg agcatgctct
     4561 ccgttctgtg tcggttgcga gactcgaaag acatttcctc acgggtctca tcctctttcc
     4621 cagattgaca gccacaagga tctatattct tctttgggga tcttcgcccc tgtgcagaga
     4681 ggtgaaatag gagtataatc tggtcatgat cctcaaaaaa ggaagtggag ggtcacttga
     4741 atcccagaaa gaattctaag ggctctttgt aaatactggg ttggagttct gaggcacggt
     4801 atcccaaggc tgtccacacc aacattcctg tgtggctcct ggaggatcct gcagcttcat
     4861 acagttatcc atgccctttc ccagtctgct tgtaatgtat ggacttcagc agccctcctg
     4921 agagttactg gctcctgcgt gcagctgtga cctgcaccat cccgggtgtg caggcaggtc
     4981 cagggtccag caccgggtgc tctcgggtgt gcaggcaggt gggaagtctc catctacagt
     5041 cacagttaca taaatagttc tgagaaagca tggtttgctc tcatcttcta ctctggtgag
     5101 tggaactcag ttaagtaaaa ccaatacaga actagaccct ggctaatgat aggtgagatg
     5161 gaggggagag aattgccttg aggacaagac actactgaat gtcacacagc ttcttgtctg
     5221 tgtaccccgt ctctgcaccc cattacagaa tgttttcaac ccctgaagtt tgtccagctt
     5281 catccccctg gcttccagcc agctttgtct cagactttat caccagccca ctctggtgcc
     5341 tatacaccca gctcagggcc ccttgacact gaagcaggaa gtacagccct ggatattccg
     5401 tagcttggcc agtctgacat gagaactgct ggagggggac cctgaatggc ttctatagtc
     5461 aaaggctgga caggctggca gcccctatgc agatgtgata tgtgacggca acctttccca
     5521 tgtcctccct ctcactgaat tctggacatc tttgtagtta tttgataaag caccttggtc
     5581 tgttgatgag tttaacccta aaccaagttc tatagagttg gagaagctgg ggagagctcc
     5641 tttatcagtg tagttcagtt atgtggtgct tataagagag ggcagaaaaa ttggtctcca
     5701 acattatgaa atgtgtgtcc atataaaggg taagtgacaa ttggtggcag tgagtagatc
     5761 aaggtttcca aacaaaaccc ctcagcatgc atcaaatcct ctgtttagta ggactgtctt
     5821 catcgtaaag acagaaacgt ggagtcatat tgcagggatc agcctttgga tttcttacat
     5881 actctgtgta tagttcagga cttagacaca gtatacacag gttcttagac acagtgttat
     5941 agccctgtaa tctcagcact agggaaacca agataggacg atcataagtt tcaggtcagc
     6001 ctgggctaca aaggaagacc ctgggggaaa aaaaaagact attttttcat atgtccctgt
     6061 ttctttgccc acaatcaaca ataatgaata ccacttatct aatagggttt gaggagtatt
     6121 aagtgagata aggcattcga aggaaccaac tcagattcac agacagagga cctgtgtgct
     6181 taaagtctaa gagtggagga agaacctgag gagcggcttc ctcagagacc ctaataaaac
     6241 cagagagcct caccagggag ctgaaaggtc cacagacagc gtcactcgga tcttatcatg
     6301 aaaccatcac acagttcctg cgaggtgggt agcatgactt tcaggtgctt gaggcagaaa
     6361 actggagctc caagaatggg tctgtattac aaagagctga gctccccaaa catggaccgg
     6421 tctcctgacc actgtcttct gtagaggatg aatacaagtc ccaagactgg agagcaaggc
     6481 tatggggagc agatggattg tattcaggtg ctgttgcaga aaaggtataa gtgaaagggg
     6541 tgcccaaggg gctggagaga tggctcagcg tgtaagagca ctgactgctc ttccagaggt
     6601 cctgagttca atttcccagc aaccacatgg tggctcacaa ccatctgtaa tgggatccga
     6661 tgccctcttc tggtgtgtct gaagacagac agtgtactta cataaaataa ataagtaatt
     6721 ctctgggagg gagggaggga gggagggagg gagggaggga gggagggagg gagggaggga
     6781 gggttaccca agccagagct gcaaaatgag aagctgtatt gttgggtgta ccatggccat
     6841 gggcatgtcc tacagaaata aatgaattag tattctaggg aaacctcctt cttgatgtga
     6901 ggacaaaaca agtctccaga tgtctccaga tgtttcaagc atgcatgtga gggctgggtt
     6961 ggagaacagc tttcaagagt ccgttcgctc tcccccatgt gggttcctga gattgaactc
     7021 aggtcatcgg attcggcagc aagtgccctt aggtactgag ccatctcact agccctgagt
     7081 tatagaatgt ttttatttgt attcatgtac aagaagaaag gtaaatttta agagaagata
     7141 gcacaacagt cactcatgat aggaggactt ttcaagtcca atgagcagtt tgtgtgttgt
     7201 aaccttatgt agaatccact tggttagccg ggtggtattg gtgcacacct ttaatcccag
     7261 aaggcaaagg caggcggatt tctgagttcg aggctagcct ggtccacaga gtgagttcca
     7321 ggacagccag ggccatccat acagagaaac cctgtcttga aaaataataa ataaataaat
     7381 atccacttgg tgattcccaa accaattctc ttctcagcca tgccctacta ccacgaatgt
     7441 tacagtttca ttgcaaggaa tcagtcaata agaaaaatat catttgaaag ggggaagggc
     7501 agagtatgtg cttttttaga aaagaataat tgaattatac ttgaggcctg caggccctcc
     7561 ctcatcacca tgaggcttag ttaggaaagt tggctactag tgctttcagt tgttgttctg
     7621 tctgctcatt tagattgaca cgggggtctg tgtgatctgg gtaacatctg tcctgctcct
     7681 gtagtagagc ccttcacaca acagctgcac aaccatcccc aggaatgtgc aactgtcttc
     7741 tgaattgttc tatttgtaaa caaagcagtc tttgcgactt ctctggttgt gtgtctgaaa
     7801 ccctgaagct gtgctgtaaa gcctgccgat tcgattcata aaccaccctg tgtctaacgc
     7861 ctcaactcat ctactcttac aggctgctcc actactcccc aacatggcag agacccatta
     7921 tgctcccctg agctcagcct tcccctttgt cacgtcatac caaacaggct ccagcaggtt
     7981 acctgaggtc agtaggagca ccgaaagagc tttaagagaa ggtgagtgtg ctagaggggg
     8041 tgggagggaa tggtgtaaga aggtgtgtgt gagcctgaag cgccatctac tcagatagca
     8101 ccgaggtgtg agtgtggggt cctttctgta agaaagtgtg tgtgcaccct tcctatttgt
     8161 gtgtgtgtac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg
     8221 aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca
     8281 cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg
     8341 ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc
     8401 tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag
     8461 gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa
     8521 ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac
     8581 aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga
     8641 tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc
     8701 tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga
     8761 tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc
     8821 tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct
     8881 acatctgcca atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg
     8941 gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt
     9001 gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt
     9061 tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg
     9121 acaaccaggt cccaggtccc gaaaaccaaa gaagaagaac gcagatcgca gatcgcagat
     9181 ctctagagtc accgagttgc tttaccggat gaagagggcc gaaacatact gtccaaggcc
     9241 cttgctggca atccacccaa ctgaagccag acacaaacag aaaattgtgg caccggtgaa
     9301 acagactttg aattttgacc ttctcaagtt ggcgggagac gtcgagtcca accccggacc
     9361 ggggatctgg actctagagg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     9421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     9481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
     9541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
     9601 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
     9661 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     9721 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     9781 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
     9841 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
     9901 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca
     9961 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
    10021 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
    10081 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
    10141 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
    10201 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
    10261 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
    10321 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
    10381 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
    10441 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
    10501 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
    10561 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
    10621 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
    10681 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
    10741 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
    10801 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
    10861 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
    10921 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
    10981 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
    11041 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
    11101 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
    11161 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
    11221 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
    11281 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
    11341 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
    11401 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
    11461 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
    11521 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
    11581 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
    11641 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
    11701 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
    11761 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
    11821 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
    11881 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
    11941 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
    12001 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
    12061 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
    12121 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
    12181 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
    12241 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
    12301 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
    12361 cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg
    12421 gtgtcaaaaa taataataac cgggcagggg ggatctaagc tctagataag taatgatcat
    12481 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    12541 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    12601 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    12661 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccggaa
    12721 taacttcgta tagcatacat tatacgaagt tatgtttaaa cggcgcgccc cggaattcgc
    12781 cttctgcagg agcgtacaga acccagggcc ctggcacccg tgcagaccct ggcccacccc
    12841 acctgggcgc tcagtgccca agagatgtcc acacctagga tgtcccgcgg tgggtggggg
    12901 gcccgagaga cgggcaggcc gggggcaggc ctggccatgc ggggccgaac cgggcactgc
    12961 ccagcgtggg cgcgggggcc acggcgcgcg cccccagccc ccgggcccag caccccaagg
    13021 cggccaacgc caaaactctc cctcctcctc ttcctcaatc tcgctctcgc tctttttttt
    13081 tttcgcaaaa ggaggggaga gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga
    13141 gtgagcggcg cggggccaat cagcgtgcgc cgttccgaaa gttgcctttt atggctcgag
    13201 cggccgcggc ggcgccctat aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc
    13261 gtccgccccg cgagcacaga gcctcgcctt tgccgatcct ctagagtcga gatccgccgc
    13321 caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
    13381 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
    13441 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
    13501 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
    13561 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
    13621 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
    13681 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
    13741 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
    13801 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
    13861 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
    13921 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
    13981 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    14041 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    14101 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
    14161 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    14221 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    14281 ttcgcccacc ccccggatct aagctctaga taagtaatga tcataatcag ccatatcaca
    14341 tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    14401 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    14461 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    14521 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gggggtaccg cgtcgagaag
    14581 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgtcgag ataacttcgt
    14641 atagcataca ttatacgaag ttatgtcgag atatctagac ccagctttct tgtacaaagt
    14701 gcactgtctg tctctggtgt tggagacctt tctgatagtc acaggatgaa actgcctggg
    14761 gattctgggg ccagactttc cccacccctt caccctttag actggggccc taatctgtgt
    14821 ggaaagtgag gtatgcagcg aatgctggag cttactgtat agtttgtgtt tgtgtgtgtg
    14881 tgaaaagagg ggagggtttc ccggctggag ggagtgagac agtgggcagg gagcagttgg
    14941 ggaaatggag ccagagcacc tggctctcag ctggccagct ccagggctcc ttacatctaa
    15001 tttccttcag gaaaactact ggaactggtc tacggaatca aggagactgt ggcaacattg
    15061 tcccagattc cagtgagcat ctttgtgact ggggactctg gcaatggcat gtcatctttc
    15121 atcaatgcac ttcgagtcat cggccatgat gaagatgcct cggctcccac tggggtggtg
    15181 aggaccacga agacgcggac tgagtactct tcatcccact ttcccaatgt ggtgctgtgg
    15241 gacttacctg gattgggggc cacagcccaa accgtagagg actatgtgga agagatgaaa
    15301 tttagcacat gtgacttatt catcatcatt gcctctgagc agttcagctc gaatcatgtg
    15361 aagctgtcca aaattatcca gagcatggga aagaggttct atattgtctg gaccaagctg
    15421 gacagggacc tcagcaccag tgtcctatca gaggtccggc tcctacagaa tatccaggag
    15481 aatatccgag agaatctgca gaaggagaaa gtgaagtacc cccccgtgtt cctggtatcc
    15541 agtctagacc ctttactata tgacttcccg aagcttaggg acacacttca taaagatctc
    15601 tccaacatca ggtgctgtga acccttaaag accctttatg gcacttatga gaagatcgtt
    15661 ggtgataaag tagcagtctg gaagcagaga atagccaacg agtccttgaa gaattctctc
    15721 ggtgtcagag atgatgacaa catgggcgag tgtctgaaag tgtaccgact gatatttggt
    15781 gtagatgacg aatcagttca gcaggtagcc cagagtatgg ggacagtagt catggagtac
    15841 aaggacaaca tgaagtccca aaacttttat actctccgca gagaggactg gaaactgagg
    15901 ctgatgacat gtgcaattgt gaatgcattc ttccgtttgt tgagatttct cccatgcgta
    15961 tgctgctgtt taagacgctt gagacataaa cgcatgcttt tcttagttgc ccaggacacc
    16021 aagaacatcc tagagaaaat cctgagggac tccatcttcc ctccgcagat ctagtataag
    16081 ggcagcctgg tacccttctt cttccacaga agccaggtta ccttagagat ggcgcaacgc
    16141 aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag ctcgccatca
    16201 gttcacatct cccctgtctc gattctaatg cattgttcca ctgagggaca gggacaagta
    16261 gtgattaaaa ttcattgacc atgattctta gatttggaat atagaaattt tgtttttggg
    16321 ctggagagat ggcttagcag ttaagaacac caactgcttt tccgaaggtc atgagttcaa
    16381 atcccagcaa ccacgtgatg gctcacaacc atccgtagtg agatctgatg ccctcttctg
    16441 agatgtctga agacagctac agtgtactta catataataa ataaataaat aaataaataa
    16501 ataaataaat ctttgggaaa aaaattagaa attttgtttt cagctattaa atgtgatata
    16561 tgtccaaatc aattttcctc tgaaacaata aagtcggttc ctcttcatta atgcctttgt
    16621 attatctctc tccttccttt ccctagatac ccctgttaag gactcatttg ttacagagga
    16681 acacttactt cacgtttgga agcagcagaa gattatggta gcaagtgttt tctggattct
    16741 atttagtact agggacagca ctccagacag tgttcctaaa ccaacctttc acacattcaa
    16801 ctgccaggtc ccaaacaaca gtgccttaag agggaacttg ggtttatgtg agtgactgtc
    16861 atagcaagag agcaatggcc tcaggggact tcgtgctaga ccatttgatg agaatgtgct
    16921 taggatatct cttccaagtg tgtgataagg gctcacagag actgccagga aagactgttg
    16981 gctcttgaag caacacagtg aagactgacc ttcgcaaggc atcaaagggc agcaaagtac
    17041 tcgaggttgg gtgatacaaa gggaaacgag gtttgtaatt cactgttctt ggatctagat
    17101 ggatggatct gtgggtagga catttgctcc tcttgcagag gatctgagat ctgttcccag
    17161 tgctcgcagc aggtggctca caactaacct gtaagtccag ggaatccaat gttatctcct
    17221 ggcctctgtg ggcatccttg cttaggtggt atatgctcac acagacatac acacaaataa
    17281 aaatgcatat tgaaggtggg tgaggtggca catgacctta atcccagcac tcaggaggca
    17341 gaggcaggta gatctctatg cgtttgaggc cagcctggta cacagtgagt tccaggacag
    17401 ccaaggctgt tatacagaga aatcctgtag ggttagcatc tgatgagggc ctcatcctgg
    17461 ataggatcag aagggctgat gcaggatcac atgtggaaag actgtgtagg aaagaggatg
    17521 ccgggcagct ttaaagacct gtctctccct tttttataac aaccaactct tatggggaaa
    17581 taatgcaatg taaaagagca ccattagtct cttccaagga cagtgacccc aggacataat
    17641 tactttcccc caggcactcc tcttaaaggt tcccctactt tcacaccacc acagtgagaa
    17701 ccataagcac atgaaacatc aggaggtgta aaacatacct accccagagc aggaaaacag
    17761 agtggtagat tgttggttct tggcaataat aaccagttac aagaaagctg cactgaggaa
    17821 aagggaagtc acgtggtccc aggtacctcg aaggaacaga agaaagaacc aaaaccctgt
    17881 ccttgctgcc ctctctactc ttcctgtcct tgctgcccac tggccagaat cccaacagcc
    17941 tctctccaga ggagttgatc tttgacattg taacatgaca tggtaaccta caaataggtt
    18001 tcattcataa ctatcctggg atgcaggcag cccaggggct ggtgcgtggc catgcctgta
    18061 aggatgagaa ggcttataga tcctgttccc tacagccagt cagtcactgg cattcccaag
    18121 atcatgaggg aagaacagat tgaatctgga cgagtctaga gaagagccag ccctggagtg
    18181 tgttaaagct attgcaaagc aagtgtgtac caatactatc ggtatacaaa gtagcaaaga
    18241 attggttcaa ggagttgttc cggatagcag cagtaaatga gctcaagcca tgatgtaacc
    18301 cagtctgttg ccttcttaag gtttccaggc ctggagctgg atgctttatg agcacccttt
    18361 tcttctcagt tcaccagtct cttcctactg tatcatttcc agtctcagct ctctcactgg
    18421 ccaagaggcc aagacaaaga gagagagcta cagccaagca tggtggtgaa cttttaacat
    18481 cccaactcca taaaaagcca agcttgcctg atgaagtagc tacaccacaa gactccattt
    18541 tatagaaaca aaacaaagga gacaagaggc tagatatgac tgtttatcac aaatctattc
    18601 ctctcccact cacaaattcg caccatgacc attgcagttg cttaatgtgt accaatgtct
    18661 gacacactca cactcttgct gctgggaatt gaactcatga cctttggaag aacagtcagt
    18721 gctcttaacc tctgagccat ctctccagcc ctgggaattc tacaccttca cccaaaggaa
    18781 gtcaggagca gactgtctta cagggagcta ggaagaaaat ctcaaagccc acctccactg
    18841 tgacacactt cctccaacaa gaccacactt cctaatagtg ccacctcctg gaccaagcat
    18901 attcaagcca ccatattgat cctcaagcag aagacaagag cctgccctca gggacacacc
    18961 ttttccaaca aggccacatc tcctcaaagt tcccaaacag ttctgtaaac tggggaccaa
    19021 gcattcaaat aaatgaatgg ggccattctc attcaacatg cacaactgtg cataaaggga
    19081 cattaagaga caaagaactg tgctatataa ggtacttttc attcttgatc tatacaaata
    19141 atttttttta aacttacact gagcatggtg gcacatacct ttaatcctgg cactcaggag
    19201 atagaagcca gtgcgtttct gtcttagtgc agcctagtct acatagtgaa ttctggaaca
    19261 gccagggcta ggtggaaaaa ctctgtctca aaaaccaaaa taaatgttca cctttaatcc
    19321 cagtactcct gaggcagagg caggaagatc tctgagttca aggccagcca ggtgtacaga
    19381 gtgagttcca ggacaaccag ggctacacag aggaaccctt tctcaaaaca aacaaacaaa
    19441 taaaaaagcc ttacagtggg actttgtcag ataagtcctt atatttttat gtactaaaga
    19501 ctcaattgtg tgaatacttc taaacaaaga agatcatttt gtaacttaat ttttaaaagt
    19561 tttttctaca tatacaatca taatgactgc atatatttat gtgtcaggag ccataatggg
    19621 acaagccagt aactcgcagg tgatcatcct gaaatggcct gcttcggatt attatataat
    19681 ctgtgacagg tgcaccctta ttaagcaagg ctgtagggat tcattttggg aaagattcct
    19741 gctattaata tgcttttcct gttattatta agcatatata ataatcacta agtaatagca
    19801 caccccttta gaacagatct ttgcagatcc atgaagatgt agacaaatag atgacttaag
    19861 tatttatgat gatcctataa gaattcctaa aattaaaaaa aaaaaaatct aaaaacaaac
    19921 tgggcagtgg tggtgcaagc ctttgatccc agcacttggg aggcagaggc aagcagattt
    19981 ctgagttcaa ggccagcctg gtctacagag tgagttccag gacagccagg actacacaga
    20041 gaaaccctga ctcggaaaaa aaaaaaaaaa aagaattcct aaaattgtat tagtgattgt
    20101 taagctcatt tatagtggga ctgccactat gtccttttct gatagtcaaa aaactacgat
    20161 gagaactctg ccagtgtccc aagtgtcacc agttaattgc tcttagatag taaccagact
    20221 ttctcccact cagagcacat tccaagaggt tgtaaaacaa ttaaccaaag accataaaaa
    20281 gagaactaat gatttattaa gaggtgctag gacagaagat agaatattga ctgggtttat
    20341 ctataccaaa ctttacttat aattcagtta tggttttaaa ccttcagtga acctgtgggg
    20401 ctgaggcagg tgatggataa ttgctggata atcactccta atggatacgc aagtaaacat
    20461 tctctgctgt aaacttctat ctcaatttac gatttgattt tttgagtgaa cttgtgatga
    20521 actttgtaac atgtgatcat gtattctgaa agatgcttaa gtgctgagga cagacagaag
    20581 agtcaggact gagggactgg ggtgagagga gtggaggtga gaggaatgga gctgagaaga
    20641 gctgagctga gaaaggagct gatttgagaa gtttttaggc agaacgcttt ttagacagaa
    20701 ctgaactcaa ggagaactta gaaatatagg catggcatca ggagaaaagg catggagagt
    20761 aagagcattg ctgtgacttc agagcaggag gagagagcac gattagaagg agtatgcaga
    20821 gtggaataac ttagaaacta taggtaacat aaaacgctaa gagggcaatg tgcagaatgt
    20881 agagggaaag agggcaaaag acgctgcaga gagcagaagg agcaggctaa ccttttagca
    20941 ttaaaagggc agaggctttt tctttctcta tgcaataaag attggaactc atttttcatc
    21001 cagaatgagt gagttcttct gcaccggtgc ttggtctttt tctccatctg cttatgtaag
    21061 tatgcatgtg tgtgtgtaag tatgtaattg tgaggatgta tgcatgtgtg tgtgtaagta
    21121 tgtaattgtg agaatgtatg catgtgtgtg tgtaagtatg taattgtgag gatgcatgca
    21181 tgtgtgtgtg taagtatgta attgtgagaa tgtatgcatg tgtgtgtgta agtatgtaat
    21241 tgtgagaatg tatgcatgtg tgtgtgtaag tatgtaattg tgagaatgta tgcatgtgtg
    21301 tgtgtaagta tgtaattgtg aggatgcatg caagttggac ccaactgggt ttgaatggaa
    21361 atgtataaat gaataaatga gcatgcatga atctgtatat gaactcatga gattatgcat
    21421 attcacttat gtaaaagttt tttcttctgt gagtgttatt ctcttatcct ggttcaatag
    21481 aagtttattg ctccaaactt ccccctagcc tgacaagcaa gaagcagctg gacaataggg
    21541 aaaaggctca agcaattatt agtcctttat ttactttatc tcctgctgtt ttaggatgag
    21601 gtgctaagcg cctgagactg cagtctctgg cctcagagga tcacagctac atagcatagt
    21661 aacttagact tagataagta aactttttat tatacagcaa ccctaaatat ctcctaagac
    21721 aaagtcttac cccccactga cactctttcc cctcccctgc ctcaagaaga actgaccaga
    21781 aagaagaacc ccacagcggg gctggtcccc ggcacttatg ggttatggca catgtgtgta
    21841 ttaggtaaca attaactcat attctatata tgcattactt caaggtcaca gcacagcatt
    21901 tttatcgatt ctgtcctgga aaaaaagact gcaggagtgt gaggcctgat ctctaggaga
    21961 cagtggggca gttagaggat ctgaggtcta acatctaaca aaacttgaag ttttccatcc
    22021 ccagcagctg aaggagaacc tcgtggccct acctagtgcc tgtttccatg tacctgggca
    22081 tgtagactaa gtcaggtttg tttgcaagcc tggccatcag cctggaccag gccagcctca
    22141 cacagaatcc atgtacactc tgactctttg gcgtattctc cctgcaatag cagcattttc
    22201 ttaacaagtc atta
//