LOCUS       KO-first_condition_ready_89885:79704_MGI:101914        22821 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Calb2 targeting project(s): 89885:79704
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 92184
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     misc_feature    5434..11415
                     /note="5 arm"
     misc_feature    11443..11467
                     /note="B1 Gateway"
     misc_feature    11480..11527
                     /note="Frt"
     intron          11528..12402
                     /note="mouse En2 intron"
     misc_feature    12400..12402
                     /note="Splice Acceptor"
     exon            12403..12575
                     /note="mouse En2 exon"
     misc_feature    12587..13180
                     /note="ECMV IRES"
     gene            13181..16241
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    16280..16523
                     /note="SV40 polyadenylation site"
     misc_feature    16527..16560
                     /note="loxP"
     promoter        16587..17106
                     /note="human beta actin promoter"
     gene            17132..17926
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    18126..18367
                     /note="SV40 polyadenylation site"
     misc_feature    18384..18431
                     /note="Frt"
     misc_feature    18438..18471
                     /note="loxP"
     misc_feature    12417..12494
                     /note="FCHK std_gpr"
     misc_feature    18487..18507
                     /note="B2 Gateway"
     misc_feature    11416..18537
                     /note="Synthetic Cassette"
     exon            18956..19032
                     /db_xref="ENSEMBL:ENSMUSE00000313226"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000313226"
     misc_feature    18538..19190
                     /note="Critical Region"
     misc_feature    19214..19247
                     /note="Downstream LoxP"
     misc_feature    19191..19270
                     /note="synthetic loxP region"
     exon            21175..21264
                     /db_xref="ENSEMBL:ENSMUSE00000313219"
                     /rank="3"
                     /note="ENSMUSE00000313219"
     misc_feature    19271..22821
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggataaatga cagctcatgg gaccttaagt
     5461 gaaagacttg tgaaatcaag agtacaagac acagtaggtg ctatgtattg cactacttag
     5521 tatgtgtgtg tgtgtgtgtg cgcgcgcgtg tgtgcacacg cgtgcacgta ttacatgtag
     5581 actaactatg ctgactgcag ttcaccagaa ccactatcat aagcccatcc taggctcaga
     5641 aaccccaggg ggcagtgcag atgactccgt gtaaatcgct ggctttgcaa acaggacgac
     5701 ctgagcttga tccttagaaa gcatacttaa aaacaaatca aagagctttg atacacactt
     5761 gcaatctcga ggtggtgaga ggaaggtggg tccctggagc tcactgacct accccaggtc
     5821 agcaagagac cctgtctaaa agaaaaacaa acagacaaac aaaaccccac aagcaaacac
     5881 caccaccacc accaaaaaga tggatggcac ctgaggagtg acacctgagc ttgacctctg
     5941 acctcagcgg cacacatacg ttggggtctc agttcattac agtcagtggg ggtctaggtc
     6001 tgggagcaag gtgttatatg catgaagatg gctttcttct aattcagtag tcaaactaac
     6061 cctgtcgcca tcttggcccc agcttggcat ccgtgataaa ggctcgctgg gaaagcctcc
     6121 tggctcacgt agggtagggt ggcttctaga atctgcctgc tccctctagt ctccttcacc
     6181 actacactga ttattccact aacgattccc tttggtgtgg cgtgtactcg gtctatcaac
     6241 tcggagccgt ggaacctctg ggtatccaac acctcacttc ttggactgcg attgcaccag
     6301 ccagaagtag ggaagctggg tctgtgaaga gcagggggag gagggctccc tggagaggca
     6361 gtcaggggca gggctgaggt actgtgttta tactgacaag atacttcagc ttgcccttcc
     6421 ccaggcagcc accaacccag gtgttctatg tccccacccc ctgcttgttc agacacaggt
     6481 gcttttatga gctaaggcct ttggggaggt tgctggggct caggagacct catagggaaa
     6541 tcttccccaa ttcccacaga gccagaatta atagagcagg cactagactc ttcaagaaca
     6601 gagcctctgc agcacccggc cggctggcat ctctcctggc ggattgcatc agatgccctc
     6661 ctgtgcccag ccagccccat ctggaccctc aggctctggc ctcctcccct cctatttggc
     6721 tctctgcagt cttacctctc tggctcaatc tgccccttcc ttctggcctc cccatgcctg
     6781 gtactcagcc cagggtcagg ctgccagctg cccacaggtt cgggggaccc agcccagctc
     6841 ccgagcccca ttacagaagt agtagattaa aaacgggagt agccaaatgt tcttctgtga
     6901 gctgcagatc tggggcattt taaaccaagg agccactagg agagaggtgt ggggagcgta
     6961 cataggcatg tgatggacag agaaatccag acaagtcgag ctatggacaa accttatccc
     7021 taagatgaac aaaaggagat tgtgatctct gttagccagg tgatggaaac acccaacctc
     7081 caccaggcct cttttccagg ctaggattat aacgtcaatg tgtttgtgta gcagaaacct
     7141 aaggagaaca gggcagaaat ggaagggctt tggaactggc cagtgcctgc tacgcaaaat
     7201 aacacaaaca ctagcaagct tcccgagcac gcggatgctg ccaccacagg aaacgagaga
     7261 aaccctgagg tgtagttaaa aagatccatg ggtgccacac ccggtgagag attcagggtg
     7321 ttgagagagg ggacctatga ttctgccctt gtgagaggtc agggttagga attaagcccg
     7381 agctggactc tgcgtttcta accagctccc aggttaccta ccgccagtcc acataaccac
     7441 gcaggaacag ccagctcttc tgtggtactc taaagttacc aaactaaact tttttttttt
     7501 tttttttttt tttttttttt ttttttttta ctttaacaag atccctaggt gatggctgta
     7561 caccttccta tttgagatgt actgccagcc tggggagctg ggaagacagg agcacctcgt
     7621 caattaagtc agagagcctg gcatggggcg caggcagtgg cattgctgtt tagcttccca
     7681 gggctctggt tgagaatggg cactctaaag agaccagaag ttgtcagaaa cccttagggc
     7741 tgaagaggtg agggaggaaa aaaggatgct ctatttatag gagatgttac attttccccc
     7801 gaacgttact ttgtatggct ctcctgatga cttatcaagc agaaagggtt taagcagttt
     7861 tgctcacttg agagagaaat gggggcccaa gaggaactgg tttgttccgg agcaagggac
     7921 agtcgtgaaa ctggaggcac agtccctctc cttgggtacc ttttgtctgc tcacctcgga
     7981 aaatctgcac ataggaacct cagggaggaa caaataaaac ctttctgggg tacagactcc
     8041 ttccgtcaag gacttccaat cctctgagct cagattaacg acaacgaaat gctctctgtc
     8101 cttagctttg gggtacacag tggtgaaatg tggaagcaaa agcaggctca ggaaggatgc
     8161 aaacctcccc ccgccccccg ctccctctca gtcccctctc cctaatgttc atggtttgaa
     8221 ggaggttagc aatgatggtt actatggtaa ccacatcttt tataaatgcc cattcacatg
     8281 agatatcctt ggtgcccccc aactccccga actgtaacaa ggtcgcagaa aaaagcaaac
     8341 cataccctca gcacttaaat cttcctagaa ggatgttggc taacggcttc tgtatcccag
     8401 ccttacatga aagggttaaa tgatggctgt ggtggccttg gagggagcac ctgtcccctc
     8461 agtggtgaca gcccttcttc tggacctgtg tgtctatttc ccacttctca ctctccacct
     8521 tcagtagcct gtccggcctt ttcaatggct ggtcacaggc ttttggacca ctctgtgatt
     8581 tgtcggaaca gggccatgcc tacagcctgg aacgattgtt ccctttgaag cagaagctca
     8641 ttgcatggag ctaaattttc tccttttgtt caggaaggca catggggatt tttatcacgc
     8701 ccccatgggt gtgtgtggga acccccttgc tgctcgatgt atccgagaag gggaccatcg
     8761 cagctcctga tcccctctcc ctgcctctaa gtttcttctt ccttctcttt cttcctccca
     8821 ttgcctggga ctgttcactg agccctttct tgtccatcct gcccaaaggt ccttggaggg
     8881 aaaacacctc tatttcatta catcatcatt tagcagtcca aggccaaagt gttcctaata
     8941 tggtgcggag gcaggagcca cagtggtgat gatcaatgcc tatagcacct gcaccattga
     9001 ggcttgtgtg ggctctgtaa gttcactgtt cttcagttgc ctctctccag tggtcacagg
     9061 aggaggaact cccgttggtc tccttttaca gttgaagaaa ctgaggttta gagaggtgat
     9121 gctcaaagtc tgcatgtccc aggaggacaa acaggtgtca gtccctaggt aggaagggct
     9181 ggtaacttta ccaaccccct atgcagcaaa tagagggaca gagaaaggac acaccatcac
     9241 ctgccatgtg aagtgggtct ctcggggagg gcttttggta agggatgctc tgcagagctg
     9301 agtggatgca gctcttcctc atgtggatgc ttcatcttcc catcatccac cgtctgatac
     9361 cctcgggaaa cacacactcc tccacacacc caacctcagc tcctgccccc aacccccccc
     9421 ccatggcctc tacattgtga gctgcgattc atcctcatag gtccagctcc taagctcctg
     9481 ccctcagctc taacaccact caccctagcc ttgggcttct gggtgacaat aaactcagag
     9541 ctgagctctg cctccctacc taactttgta aagcctccta ggggaagagg gtggggacca
     9601 aggctctgca ctcagccatg gccaagggga gtctgcatct cagccatggg ataattgtca
     9661 ggtcagagag atatcactag gtaaccactc aggattgtca ccaacatctc tgcttctttg
     9721 cctagaaagc tcctggtcac cacacttcac cccagctggc ctcacaggcc aacaagcccc
     9781 accccccttg tgaatttcca actctcctga agtactttgt agaacatcct agaagaaaag
     9841 acaattgcct cctcccagag gggcccagta atcacctttt ctttgcggac aaaaataaat
     9901 gcataattca tctcatgtaa ttctctctat actagattta ttggagcatc ttccccagga
     9961 ggagtggcct ctgaggttat ttattttgga cttaagacct cagggacact aggaagagag
    10021 gttggaaaga tcctcccatc cccatgttac ccacagaagc caaggcaaat ccttctgtgt
    10081 ggttctttta ttaagtctgc agctccacac gcccttgaag aaagtgatca ccttcttaac
    10141 cattcaggtt gggcctcctc catggcgacc ttgctcccct gctattgatc agattaggaa
    10201 cttgatgtta gttgattgtc taacattgct gtacaaggaa atcatctggg agccttaaga
    10261 aagaaaaatc acgatgttga gttcacagcc caagcgagtg ataccaggat gtctagtggt
    10321 gagagccaga cattcatatt atttaatgat cctcaagaga tcccaacatg cagtaaattc
    10381 tagagccatt gccttggccc tgcctgagtt cctcccgtaa gtgctagcac agaccggtga
    10441 ccttggccat agactcatct cagcaccagt gtctggacca ttcaggctcc agttgtgaga
    10501 acctatatga cttctcactc atgtgatctt cagacaagcc aggccttgac cttcagacct
    10561 ggctatgatt gctgcactct ttcctcaggt ccattcccat catgaacaca gactgtgtgg
    10621 tctctatgca gaaagctccc acctcgaagc tggagagact actgcacaag catgaggacc
    10681 tgagtttgat tcacagcttc tgtgtcaaag ccagatgtgg ctgtgcacag cagtagctcc
    10741 agaacttttg gggagagaca ggcagatcga taaattcatg aggatctagc caaaataatc
    10801 agatccaggt tcagtgagag aataaggcag aaaatgacag agggaggtat gcaacattga
    10861 cctttgtttt catatgtgca tacaacctgc gcatccatgt gtgcatacac ttgttctctc
    10921 tctctctctc tctctccaaa agtctcacaa cataagccac atgactataa tcttacaacc
    10981 tgggaggcag aagcagtaag gttgtaagtt tgagaccagg ctgggctata tggtaagacc
    11041 ttgtctaaaa taacaaccag cagaactatt taactatagt aagccattct gcagatgaga
    11101 tggcttgaat aatttttccc atgggtccct tctatatttg tttcttgaga ctgccacagc
    11161 aaagtactac agaatgggta atctaacaca atagaaataa atctttctag tttttgatgc
    11221 tagaactatg aaatcaagtt tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
    11281 tatgtgtgct tatgcataca ctataggctt tgactgttgc atgcctctgc ttctagaggc
    11341 agcagcaatc cacaatattc tttagcttgg aaacatgttg ctctaatctt tgcctctgtc
    11401 ttctctatgc tttgaaaggc gcataacgat accacgatat caacaagttt gtacaaaaaa
    11461 gcaggctggc gccggaaccg aagttcctat tccgaagttc ctattctcta gaaagtatag
    11521 gaacttcgaa ccctttccca caccaccctc cacacttgcc ccaaacactg ccaactatgt
    11581 aggaggaagg ggttgggact aacagaagaa cccgttgtgg ggaagctgtt gggagggtca
    11641 ctttatgttc ttgcccaagg tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt
    11701 ctggggtaga aggtgagagg gacaggccac caaggtcagc cccccccccc tatcccatag
    11761 gagccaggtc cctctcctgg acaggaagac tgaaggggag atgccagaga ctcagtgaag
    11821 cctggggtac cctattggag tccttcaagg aaacaaactt ggcctcacca ggcctcagcc
    11881 ttggctcctc ctgggaactc tactgccctt gggatcccct tgtagttgtg ggttacatag
    11941 gaagggggac gggattcccc ttgactggct agcctactct tttcttcagt cttctccatc
    12001 tcctctcacc tgtctctcga ccctttccct aggatagact tggaaaaaga taaggggaga
    12061 aaacaaatgc aaacgaggcc agaaagattt tggctgggca ttccttccgc tagcttttat
    12121 tgggatcccc tagtttgtga taggcctttt agctacatct gccaatccat ctcattttca
    12181 cacacacaca caccactttc cttctggtca gtgggcacat gtccagcctc aagtttatat
    12241 caccaccccc aatgcccaac acttgtatgg ccttgggcgg gtcatccccc cccccacccc
    12301 cagtatctgc aacctcaagc tagcttgggt gcgttggttg tggataagta gctagactcc
    12361 agcaaccagt aacctctgcc ctttctcctc catgacaacc aggtcccagg tcccgaaaac
    12421 caaagaagaa gaaccctaac aaagaggaca agcggcctcg cacagccttc actgctgagc
    12481 agctccagag gctcaaggct gagtttcaga ccaacaggta cctgacagag cagcggcgcc
    12541 agagtctggc acaggagctc ggtacccgga agatctggac tctagagaat tccgcccctc
    12601 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
    12661 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
    12721 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
    12781 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
    12841 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
    12901 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
    12961 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
    13021 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
    13081 ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
    13141 gttttccttt gaaaaacacg atgataagct tgccacaacc atggaagatc ccgtcgtttt
    13201 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    13261 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    13321 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
    13381 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
    13441 gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
    13501 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
    13561 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
    13621 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
    13681 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
    13741 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
    13801 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
    13861 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
    13921 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
    13981 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
    14041 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
    14101 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
    14161 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
    14221 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
    14281 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
    14341 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
    14401 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
    14461 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
    14521 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
    14581 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
    14641 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
    14701 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
    14761 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
    14821 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
    14881 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
    14941 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
    15001 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
    15061 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
    15121 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
    15181 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
    15241 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
    15301 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
    15361 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
    15421 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
    15481 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
    15541 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
    15601 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
    15661 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
    15721 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
    15781 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
    15841 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
    15901 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
    15961 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
    16021 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
    16081 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
    16141 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
    16201 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcagggggga
    16261 tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag aggttttact
    16321 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    16381 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    16441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    16501 tgtatcttat catgtctgga tccggaataa cttcgtatag catacattat acgaagttat
    16561 gtttaaacgg cgcgccccgg aattcgcctt ctgcaggagc gtacagaacc cagggccctg
    16621 gcacccgtgc agaccctggc ccaccccacc tgggcgctca gtgcccaaga gatgtccaca
    16681 cctaggatgt cccgcggtgg gtggggggcc cgagagacgg gcaggccggg ggcaggcctg
    16741 gccatgcggg gccgaaccgg gcactgccca gcgtgggcgc gggggccacg gcgcgcgccc
    16801 ccagcccccg ggcccagcac cccaaggcgg ccaacgccaa aactctccct cctcctcttc
    16861 ctcaatctcg ctctcgctct tttttttttt cgcaaaagga ggggagaggg ggtaaaaaaa
    16921 tgctgcactg tgcggcgaag ccggtgagtg agcggcgcgg ggccaatcag cgtgcgccgt
    16981 tccgaaagtt gccttttatg gctcgagcgg ccgcggcggc gccctataaa acccagcggc
    17041 gcgacgcgcc accaccgccg agaccgcgtc cgccccgcga gcacagagcc tcgcctttgc
    17101 cgatcctcta gagtcgagat ccgccgccac catgattgaa caagatggat tgcacgcagg
    17161 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    17221 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    17281 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    17341 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    17401 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    17461 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    17521 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    17581 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    17641 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    17701 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    17761 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    17821 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    17881 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    17941 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    18001 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    18061 cagcgcgggg atctcatgct ggagttcttc gcccaccccc cggatctaag ctctagataa
    18121 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
    18181 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    18241 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    18301 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    18361 tggatccggg ggtaccgcgt cgagaagttc ctattccgaa gttcctattc tctagaaagt
    18421 ataggaactt cgtcgagata acttcgtata gcatacatta tacgaagtta tgtcgagata
    18481 tctagaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag taggcggaat
    18541 ggttgctggg aggacactct ccaactctat acaccttctc aagcttagaa acccaacttc
    18601 tgccctagtc gcaggcctac tacctgctga caaatcctga tgtagacgat gcttctcgtg
    18661 ggacctctag cacacagcgc ttgctcagaa acagcagcca tctgtgccag cggaccagat
    18721 gtccgttccc ccagtgttct gcaggcgctg gctgagtact ccaagggtac acattattaa
    18781 tcttgaacat gtgcctgcaa gtgcaccgag gacaaggacc catccttaac gtgactggcc
    18841 aagatacctg tttgcagacg agtgggggga aagttctccg tggaggtggt gacttcctag
    18901 tcttattatt cagctctaag gttaactctg atattctctt tatctatcgt tacaggaaat
    18961 gggtacattg aaggtaaaga gctagagaac ttcttccagg agctggagaa ggcaaggaag
    19021 ggttctggca tggtaagctc cacccccgtg tttgggattt tcttgtcatc ttgacctatc
    19081 tagtcttgag aatggtctga cagaccatac atctagagac ctggggtctc tccaatcttg
    19141 gcactaaaga gtctttcgtt tgtaaggata taaagaaaag ccaggttctc gagatggcgc
    19201 aacgcaatta atgataactt cgtatagcat acattatacg aagttatggt ctgagctcgc
    19261 catcagttca tctatctttt cccgaaaaca ctgcccagaa ggccaagggt ttataattca
    19321 cgagggggtt ctcagaggac cccaaaatgc ctgcaagtat cccagcctca gcactgttgt
    19381 catctgaggc tgaatagttt ttgtcataag ggatgtcttt atgttatagt gtgttatcca
    19441 gcacgtttgc acctacccag gagatagcca agtcataggc atcaaaaatg tcctaagttg
    19501 ggttgtgatg gtgcaaacct ttaatcccag tacttgagag gcagaggcag gcagatctct
    19561 gtgagtctga ggccaatatg gtctacaatg tgagtcccag gatagccagg gctacagagt
    19621 gagaccctat caaaacaaac aacaaacaca ccactaaaaa caaaacaaaa caaaacaaaa
    19681 tgtcccagac attatcaaat gtctcctggt ggagaagagg ggacagatcc agcttctaga
    19741 ccaacaaagg ctagcttctc tgtaaggaaa actaagggct actcagaagc accccctgag
    19801 ccctgaccat cctgttggta tggaggagtc atgacctttg aaccagctgc tggtggggtg
    19861 tggatgtgct tgcccacaga tctctcatca gccctcgtga ctaggatgat atgagcccag
    19921 gatacagaaa tcctggcttt tccaactgac tctgggatca tgagctacaa cacagaagaa
    19981 tgtgtttaat ccagagtttc aaatctaacc atcatcctct tagtcattct ctccctctgc
    20041 cagcacgagg gtaactgaca agtctgtcca ccgcaatccc aattgctgtc atctgggacc
    20101 gattcccaga gcatctgtca tgtggatttg gggggcagga gctaggcttt caatctctag
    20161 ctgtatttac ctgctgggtg aacatgaggg aagtcactgc atcttggagt ccatttacct
    20221 gtgagatgca gagatgactt gcccactggg gaccgtggta aacctctact aggctgccca
    20281 catttataat aagtagttgg cgtcagatct tacgtatttg taaatataga taaacactga
    20341 ctgacgagga ttggtacatt gagatcagat tcaaaggcca gaagtgatac agaagaatcc
    20401 agaaaaagct ctcaggattt gtagaatttc tcttggatca atcccaagaa gctcgaagcc
    20461 tcggtggaag ggccccgggc aagcagcaga agagtagacg gtcctggggc aggggagggg
    20521 ctgcagcatt ggctgaggat aagatagagt tcctgggtgc aggtgacatc tcagccgcac
    20581 ccaaaaactg gggaaaggtt ccagaccgtc aagagaccac tatgtttaag ttgacgggta
    20641 actggctaac aggactctta ataagtaatc ggtttttcta tccttagaat agttgagtgg
    20701 cggaatttaa atctcccctg agctgtgtga tgccctcaca tcccttctag cgtgaaatca
    20761 gataagagta ccgcagaatc gcaagtgtat ctgtaaagag gcgaatgagc gcttcatttt
    20821 gtattggtga aagtggtcgg aacctattgt ggtgtggtgg gtggagcttg gttgtgctaa
    20881 gagctgtaac tcccatcaga gtggtggaaa taaggccgaa cccattatgg agctgtggat
    20941 ctcaaacgtc aaagggtgca tctaatcgca tggtacagaa catgcccacg tgtcctgtgt
    21001 cccttgataa tcatgcgact cccatctaaa gcacagactg accctcggag caccagggcc
    21061 acacgtttct gagccatgtg ttcaggactc aaaacgaagc tcagccctgc ttcactgaga
    21121 tcctcaactc tacataacat ggtagcagga tcagcctcct ctctctgtcc acagatgtcc
    21181 aagagcgaca attttggaga gaagatgaag gagttcatgc agaagtatga taagaactca
    21241 gatgggaaaa ttgagatggc ggaggtgaga gccagggggc gtggctgagg agagccaggg
    21301 ggcgtggctg aggagtgcca ggggtccggc tgaggttgta acaggttctt gtgtccaaga
    21361 aacaagaaat atccctgccg aagcacttaa gccaaccgaa cagaatgaat aggggctttc
    21421 tctgtgtgtc acccaaggaa agagcaagga acaaacaatg gtctaggatc tggtctctaa
    21481 atgctctcag gatccaacag ttgctgtaaa tgtgtgaaaa gccaagctta ggacagtggg
    21541 aagattagta aataggagtc tgcatacccc acctaaacgc atccaaaaat gaatatatat
    21601 attagataat ttacatgcat gacatacaat ccatgtaatt tctacatgcc tacatataca
    21661 tacatgctca gccacacgta tatgctcata cttatacgta aatatgtatg ccaaccatga
    21721 tcatcagttt gtgacctgtg gtcgagaggc caggaatggt agtggtagcc cagaaatcag
    21781 ggccttgacc tcctaggata gattggttca aattctacga tagaccagac gaagctctag
    21841 aaatgaagct tgagacatgc ggcatgcatg ttgagacatg cggcggagat cagacaggac
    21901 gagaggattg tttgcaaagt gcagagagaa gggtacgctt ggagaccatc tctgccttgg
    21961 cctatctcca atagaactct ccgtttcacc tggaggccca tattaataag tcattgggta
    22021 caggctgctc tgcccaagaa ttgtaggaac cacagacctt ccccagtagg gctgttctat
    22081 tttagctgag ggtagttcac ctgcggggga agttgtatct agctggcagt tgcccttcgg
    22141 gtcaactgag gaatgtacac aaatcaactt aaaagaggag gcatgataca acgtgaccat
    22201 tgtaaatctc agggtcgcaa gacatcttta ttagatacaa cctgtaagaa atatttcagg
    22261 gtttacgggc ccgtggtgtc tctgcccaca gctcttcaac actgccgctg taacagggag
    22321 aagatactgg aaataagccc cagaatgggt tggggcgtgt gtgtcactag aactttacaa
    22381 acgtgccttg tgtcctgtgt ggcactgtgg tttacttatt cctcatttaa gccaagagac
    22441 tctcctaaag aacgagtcca tggtcaaggc tctgcctctc ttctcttggc tctgttccct
    22501 gctttaaaac aacaacgaca acagacaaaa acaaaacacc accaccacaa caacaataac
    22561 agcacaccat tgtgttcccc aatcttgtag ccagaggact gcaaacactt cagaaatatc
    22621 atgcggacac agccatgttc atttgcagag tgcgtcccct ctttaaatgc aaggaaactt
    22681 tctccacaag ccacagtagc ccaccttaca tgtcattggc cactttgggt cacgtgccta
    22741 ttccaaagcc aatcgctggt gaggggaatg ggattgtctc atttgaggga ggggttgatt
    22801 aaactaatta ggatttgcct t
//