LOCUS       KO-first_condition_ready_63827_MGI:101912        24575 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Dad1 targeting project(s): 63827
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 212975
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     misc_feature    4215..9559
                     /note="5 arm"
     misc_feature    9587..9611
                     /note="B1 Gateway"
     misc_feature    9624..9671
                     /note="Frt"
     intron          9672..10546
                     /note="mouse En2 intron"
     misc_feature    10544..10546
                     /note="Splice Acceptor"
     exon            10547..10719
                     /note="mouse En2 exon"
     misc_feature    10731..11324
                     /note="ECMV IRES"
     gene            11325..14385
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14424..14667
                     /note="SV40 polyadenylation site"
     misc_feature    14671..14704
                     /note="loxP"
     promoter        14731..15250
                     /note="human beta actin promoter"
     gene            15276..16070
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16270..16511
                     /note="SV40 polyadenylation site"
     misc_feature    16528..16575
                     /note="Frt"
     misc_feature    16582..16615
                     /note="loxP"
     misc_feature    10561..10638
                     /note="FCHK std_gpr"
     misc_feature    16631..16651
                     /note="B2 Gateway"
     misc_feature    9560..16681
                     /note="Synthetic Cassette"
     exon            17038..20245
                     /db_xref="ENSEMBL:ENSMUSE00000753567"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000753567"
     misc_feature    16682..20463
                     /note="Critical Region"
     misc_feature    20487..20520
                     /note="Downstream LoxP"
     misc_feature    20464..20543
                     /note="synthetic loxP region"
     misc_feature    20544..24575
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaatctta tatattagtt ttttactagc aaagtgagag tagccaggag
     4261 tctgttcatc ttctagtggc tcacacctgc tcctgtgcct ctgctgtttg attccctccc
     4321 atgtcagctc ttgcacttac atacacagag gcaccagtct tctcttggtg gctgaacagc
     4381 ttctgtggtg atcccttcat tcagcattgt gggttttctc tattgagtca ttctcaagtt
     4441 ttagacttgc tctaatattg tctaatctta cagttgaacc aggcacggtg gtgcatactt
     4501 ttagtcttag ctcttgagtg actgaggcag gaggatatct atgaatttga ggccaggctg
     4561 gtttacatag tgagttccag gccaaccaag accctgtctc aaaaaggaaa aagaaaataa
     4621 gttccagtcg atcccaggca ctgctagcaa ccatcgctag catcttttcc ttctcccttg
     4681 ttgtagagct tcttgaagga gtagttgctg tgtctccctt acccccacat atttctcagt
     4741 gctcttgtgc tgtggcctgt ccccaccact tcttcaaact attttcccat gtggccagat
     4801 ggagtagtca tctgcctcac tttacctgac tgctcagcat tagtatttag ctcaaatggc
     4861 tggttctttc tgagtgaaac actcgcttct cagcgctctt gtgactgcct cttcctcgtt
     4921 cctcctcacc tcctgctggc ttttccttcc cactgtgctg ggcacttcat tttgtatttc
     4981 tttccatagg tgaatttttt tttttttttt ctcaacaggg tttctctgtg tagccctgct
     5041 gtcctggaac tcactctgta gaccaggctg gctttgaact cagaaatccg cctgcctctg
     5101 cctcccgagt gctgggatta aaggcgtgcg ccaccactgc ctggcaccat aggtgaattt
     5161 aagttctgta tcctgtccag ttggttcaaa ataccaaatc cacttgctga cgtatttctc
     5221 tttgatgtct cttcaatcgc tcatgtttac atatcccaaa tggacattcc ccttaccctc
     5281 cttcccagcc atctgctttt ttaccagcca gtgtacagtg tcagccctgg gtaggttgtt
     5341 cagccagata tataggaggt ctttttgatc tcataatttc tctcaactcc cacatccatt
     5401 tcaccagcaa gctttgtctt ctctggaata taggctgtct ctgtctctgt ttctgcctac
     5461 ttttaccacc ttaaccctga gctcttcatt tcctaagtca ttcatcttta gtcctgcaaa
     5521 ccattcttat gttgcagtct ggccttttaa agaatgtgag tcgttgttgc ttggctagtc
     5581 gaacccttga ctgttgcttc attgcccttt ggctaagtct agactctgtc ctgagtcctc
     5641 aatatcagtg accagatagc ttggaagtgg caagactgtt tttactatct gtattgctct
     5701 gccccagatc tttatgtgcc tggctcttta gcctttaaat ggtctttagt agcattttta
     5761 aagacacgac tactggatac aacagccttc tagtctcctt atcttgctac attttctcca
     5821 cagtgtgtac cccacacaga atttccttgc tggtttgcag acttgtgact ggagacactt
     5881 gtttgtctcc acggactata ggtccctcgg gagccagagt cctgtatctg gtcctgccat
     5941 cttcccagcg tctagagtga tgcttgactc atagcaggcg cttgttacag cttttgctaa
     6001 acacatgagc ctttaaggag atgatgagca gaggtgctgg atgggcagtc aggaggctgt
     6061 cggtttccgt ctctggaagt cctcagggag acgctgaacc ctcatttctg gagcactttc
     6121 tttatctgtc tgtgtccaag ccagctgagg gatatggcat acttacgagt gacacaggat
     6181 ttggagggtt ggtggttctc atgctcatca aatccaggac ctggtaagtg tccttcagac
     6241 aatttcccag gctcttcttt ggaggctgct tccagctcac ctgtctccca ccacttcctg
     6301 gacattgctc tagtttgctt tctatggctg tgataaacta cgaccagtag caacctgggg
     6361 aggaaagggc ttatttgact tacatggcat agccctttat caagggaagt aaaagcagga
     6421 actgaagtca agaccatgga ggactgctgc ttactgcttg ctcaactcgt tccttacaca
     6481 accaggacca cctgtctagg ggggatagtt gcactgccta cagtgagctg ggtccttcca
     6541 tattagtcat taattaggaa aatgcaggag ggatgactca gcagttatga atacttttat
     6601 agttgctttt ctagaggacc caggttcgaa ttgaacctgg caccttgagg gggtttgaca
     6661 tgctcttctg gcctcctatg acaccaggta cacaagtgat acacagactt acatgaagac
     6721 aaaataccca ataaataaaa taaatttttt aaaaggaaaa aaaaatgccc ccaacccccc
     6781 cagcctagtc tgatagaggc aacccaaagc cccacatgca gttccctagc tgaggttctc
     6841 atttcagctg actagtttgt gtcaagttgg caaaaagaaa aaaagaaaag aatccaacta
     6901 gcataggcat tttatgcacc agatggggtg tttgaagggc tagagaatgg ctcagcaatt
     6961 cagagtgttg gctattcttc cagaggaccc aggttccatc cccagcaccc acatggtttc
     7021 tcacaaccat ttatgtaact tcagttccgg ggtatccagt gctgtccagt aacctcaatg
     7081 ggcaccaggc gcacacagat gatgcatgta tacacatgta ggcaaaacac tcatacacat
     7141 acactaaaca agtttttaaa aatggggtgt tgatctcaat ggcctctgag gtccactgga
     7201 tagttgtggg gttgacttgt gcactgtaac ttaatttgag ttgttatagt cattttaagg
     7261 tgactagcag ctcatgactg tatccactgg cacaaagctt ttctcttgtc ccctcatagt
     7321 tactccagac actacctcat tttctatccc agtgctgtta acagttctgt atctatttct
     7381 ctagagtttt tgctagctga caagtaagtg atagactaga tgtaaagtaa atatgaacat
     7441 agtaattata tataaaagtt acagttttag aaagagcagt ttggggccca cggcagagca
     7501 gctgcagaag caggcagacc tgagttaagg tcccagcact ccaggtctgt cacccagggt
     7561 ggaggaaggg gaaggcaggc agaccttgaa cctcattggc tagccagacc agggagtcta
     7621 agcttcaagt agaatgtctg tgagaatcca tgagttcata agaactccct tctcaaaaat
     7681 aaggtaggtg gactggaaag atggctcagt ggttaagatc acttcctgct ttcagaggat
     7741 ctgagtttga ttcccagaat ccacatccaa cctccaggaa tgccctcatc agccttttag
     7801 gagcacctgt atacacaagt catatagaca tggcacacag tgaagtgcgc gtgcgcgcac
     7861 atacatgcac acacagatac acagagacat acacacagat acacacacac acacagatac
     7921 acacacacac gcatgataaa aatgaatctg aaaaaaatta ggtgggatgt gaatgaggaa
     7981 gattcctgaa tttaacctct gacctccaca cacatgtatg tgtgcaccta cccttatgtt
     8041 cagcacatat gaacatgtac atatacatac acaaaataca gacatttaca cgcaatacta
     8101 cagacacata caaaaagaga gctgacatga gttagtgatt ttgactattt ttttcactca
     8161 gtgaaagtgg atagctttct tgttagtgta tagagaattt cccttagtct gaataattgc
     8221 ataggatttc attgcaggtg tgtagtatga tttaattatt ccccttggtg acatttggag
     8281 catttctttt tgttttcttt caattttgca aacctactgt gttttgtaag acacattcat
     8341 tgttaaaagg taaaaacatt ttaaatacta agtggtattg ccagtggctt tcccccagag
     8401 gtggactagc ctggcctttt acagcaaggc tttaagtggt tttcttactc cagccagtgc
     8461 ccttcataaa ggtcagtgca cttccgcagt gtgtctgtgc ttaggttgat gggaactgtt
     8521 gccatggtga tgaactctgg aggactaatc tgatcattct gtcttctcag tggattcttt
     8581 gttaatgttg gagtggatgc tagcctgaag caggtgcaat aatttattga agccataaaa
     8641 ggtgtttgag cagagaatgc ccctggagct gcttttcatg tctgggtgac tggctgccgc
     8701 ctcgttagag acttcaggga tacagaaacg tgggagtaga gatcactgtc aagagtcttg
     8761 tgtgttggat cttctgttgt cttttgtgac aaataaagcc actagaactt taccaaagac
     8821 agtgtatcta cagcaggagg cccttgtgga ccttcagctg tactcctgat ctctgccgtg
     8881 tatcttctgg tagaagatga ttaagatgct atagggtcat cattcatccc agtcttcgtt
     8941 actgctgcat ctgtagcccg tgtaggcata attagacacc ttgaagtcaa ccagcttaag
     9001 agtgcttagg cagttaggag gtgtgagtaa gcttacagct ttagcacctc tgatgtgttg
     9061 ttttccccct ttattgttcc tttaataacg gaaagttaag attgtaaagg tatataattt
     9121 tatgagtttt agttttctat ttactttttt cagtttttag ttattggatg tgtgtgtgct
     9181 gtcaggcttg tcggcaaatg tcctccccag aaagctacct cacaatctta gtgtgatatt
     9241 ttttgagaca gtgtctgatg tagctcaggt tccccttgga cttgctgtgg acttggagat
     9301 gaccttgaat gattcgccct tacacctgct cctgcagtgc caggctgcag cggtgcagca
     9361 ccatacctag tttggggtga ggttttagac actccggtct aactatgatg acttggatca
     9421 ggacagaacg ttatcactac tgtacaggtt tcttgcattt taagccatga ttctgtcctg
     9481 agagatgaaa gtcactatct gtcttatgct actgtagatt tactcagcct attttgttta
     9541 tttgtttttg ttgtcttgaa aggcgcataa cgataccacg atatcaacaa gtttgtacaa
     9601 aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt
     9661 ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact
     9721 atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg
     9781 gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca
     9841 aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc
     9901 ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt
     9961 gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc
    10021 agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac
    10081 ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc
    10141 catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg
    10201 gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt
    10261 ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt
    10321 ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt
    10381 atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca
    10441 cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga
    10501 ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggtcc caggtcccga
    10561 aaaccaaaga agaagaaccc taacaaagag gacaagcggc ctcgcacagc cttcactgct
    10621 gagcagctcc agaggctcaa ggctgagttt cagaccaaca ggtacctgac agagcagcgg
    10681 cgccagagtc tggcacagga gctcggtacc cggaagatct ggactctaga gaattccgcc
    10741 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
    10801 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
    10861 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
    10921 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
    10981 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
    11041 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
    11101 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
    11161 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
    11221 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
    11281 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aaccatggaa gatcccgtcg
    11341 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    11401 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    11461 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
    11521 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
    11581 ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca
    11641 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
    11701 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
    11761 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg
    11821 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
    11881 gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
    11941 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc
    12001 gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt
    12061 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg
    12121 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
    12181 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
    12241 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
    12301 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
    12361 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
    12421 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
    12481 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg
    12541 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
    12601 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc
    12661 acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg
    12721 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg
    12781 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
    12841 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga
    12901 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg
    12961 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact
    13021 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg
    13081 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg
    13141 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc
    13201 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
    13261 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc
    13321 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga
    13381 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag
    13441 aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc
    13501 tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg
    13561 agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga
    13621 ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg
    13681 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct
    13741 ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac
    13801 ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat
    13861 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg
    13921 ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc
    13981 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc
    14041 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
    14101 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc
    14161 agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa
    14221 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
    14281 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga
    14341 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggg
    14401 gggatctaag ctctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt
    14461 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    14521 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    14581 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    14641 tcaatgtatc ttatcatgtc tggatccgga ataacttcgt atagcataca ttatacgaag
    14701 ttatgtttaa acggcgcgcc ccggaattcg ccttctgcag gagcgtacag aacccagggc
    14761 cctggcaccc gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc aagagatgtc
    14821 cacacctagg atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc cgggggcagg
    14881 cctggccatg cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc cacggcgcgc
    14941 gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct ccctcctcct
    15001 cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag agggggtaaa
    15061 aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa tcagcgtgcg
    15121 ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta taaaacccag
    15181 cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag agcctcgcct
    15241 ttgccgatcc tctagagtcg agatccgccg ccaccatgat tgaacaagat ggattgcacg
    15301 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
    15361 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
    15421 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
    15481 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
    15541 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
    15601 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    15661 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    15721 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
    15781 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
    15841 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    15901 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    15961 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    16021 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
    16081 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
    16141 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    16201 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccccggatc taagctctag
    16261 ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
    16321 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    16381 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    16441 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    16501 tgtctggatc cgggggtacc gcgtcgagaa gttcctattc cgaagttcct attctctaga
    16561 aagtatagga acttcgtcga gataacttcg tatagcatac attatacgaa gttatgtcga
    16621 gatatctaga cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg
    16681 gtttgcctgg tgttaaggct ccttatgtat gaatcatcta gtgtgcactc ttcatgtttg
    16741 agttgtgctg catagcgtgg tgaattccat ctaacttgga tgtagcagaa gttccttttc
    16801 attgccgttt agttttttct tgtctacatg tggtatgtag tctccctgta ctgttaatag
    16861 acattaactt ctagacggtg tacagacatc acaggggtca ctcctgtgcg tgcctcttgg
    16921 ctgtgctgct ttcattttat tagagacttt ccatcacggg atatatttgt gtattgatta
    16981 gcaatcattt gttttatatt tctgaataat gacttttgtg ttttgtttct tacccagttt
    17041 gcctgagaat acagatcaac ccccagaaca aggcggactt ccaaggcatc tctccggagc
    17101 gagcctttgc tgacttcctc tttgccagca cgatcctgca ccttgtcgtc atgaacttcg
    17161 ttggctgaac tccgtctcct taccgtggag ttggaggttg gcggagcgta agtggccatc
    17221 ccagccttgt gctgctgtgc tgattccagc aggtggatta gatgggctgg ttctggtcct
    17281 ggtcggccga gttcccatgg tacagtaggg tgtttgcagg cttgcccagc ttcagcgggc
    17341 agcagtttca tcgcgctcgt cagcctgtgg gttacaggtg tgctcccaaa ggcttttgaa
    17401 gaaagataaa ctgcaagttt tgcttttaga tttgttcttg agttttttcc caattctgat
    17461 tgccattgat gttaaatatt aattatggcc attgttgcct ggtaggtact cagagtagtg
    17521 tgaatgtatg acagctgctt cttaggaatg tgtgccctga actcaaatgg taaagttgga
    17581 ttaacttatg agaagactta ggaccagatg ggtaacattc ggtttgtttg cttcatccct
    17641 tgttaagcac gaagccatct tgcccccccc aaacaaactt tacagaattt taatagggaa
    17701 atgcaggtag gcctagatct cttttgtaga gctgaaaatt ctctctgccc tcccttcata
    17761 ggttgtgtgc cctcctctcc actctcccgt cttgtctcag ttacttcatt aatgctgtga
    17821 caagacacca tggcagaggc agcttgtgga agaaagtgtg taatttggta ttgattgctg
    17881 caaagagtta gagtctatgg ctgtcagggt ggggagcatg gcagcaggca ggcttggtgt
    17941 tagggcagta gctggcagct tacatcttga gccacaagct tgaaacaaag ctaactgaga
    18001 gtggcatggg cttttgaacc tgaaagccta ctcacagtga catacctcct ccaacaagga
    18061 cacacctcct aatccttccc aaacagttcc acctactggg tggaagcatt caaacatagg
    18121 aacctgtggc cattctcatt cagccctcta catcccagaa gcagagtctt aaagcagtga
    18181 aacggacatg atttttccca acacatattt ttccttgatg agagaaaatg agccgggaac
    18241 aatgcagttg cttcccagat gcacaggcgc acgatgtaga gtatcctctg ccgcttgatg
    18301 tttggcccta tgctcatgca actcagggac gaatgcctgt gcttttcctt tcctttgcac
    18361 aataagattt cgtactattg cttccaggtg gcaagagaag agaatcttag gaaataataa
    18421 ccttttggga gaaaatgtcc caggtccacc ctctttacat cactacatgg aaacctgtgt
    18481 gttggtactg agagcctcag aaacctcaca cgagaaccag ggatgcatgg tgtgtgcaga
    18541 atcgcccagt cccagatcgc agtgcccgtc ctttgttgaa agtagtaggc tcctagaaaa
    18601 ggtggggaaa cgctcttgtc tggggatcgc cctgcacatt ttccagagac ctttgacccg
    18661 atacacgcta gctaccctgt ggtcccacac atctgtctgt cccatctgtg tggttttcat
    18721 tcagctgtgt gctctaatgg tcacactaac tcagaaggta ggatgaaaag gaaaacaaaa
    18781 acaagaccac tcagtgcgct tataaaacac tcattcctaa gacactggcg gtgttgatgc
    18841 ccttattctg tgaaatgaat tacggggcta tagggcagca cagttggggc tagattctca
    18901 tgttgctgcc taacagtctc cttattttcc agttgacaga tgggcatttt aaaaaattta
    18961 atgaatttga aggtggtatt taaaaccagg aagaaaaggc atatcaaaag aaaatggcat
    19021 tgcgctgtaa aagtcagtgt gacttagggg ggccagtgaa gggcagttgc ttcaagtggc
    19081 gctcactgca tgtcctacaa gtgcttgtga gtatcatttg actttcaaat ggcctcaagc
    19141 aggcgagcct gccattccca tttgcagact gggcactgag agacccagag agcacaagga
    19201 gaaatctttc atttgttttt gctttgaaac agactctcat gtaggacaag gtcgcttcaa
    19261 atcagttatg tactgaagga tggccttgtg ttcttgaccc tccaaccctt gccacgtgct
    19321 gggattgcaa gtataggtcc tcagttcctg ggcccaattt tagatccttt ttctaattta
    19381 aaaaatttta attttaattt gcatatatgt atatgtcttg tgggtttatg tatgttagtg
    19441 caggttccca tggcgaccag aagtgatggg tctcctggag ctagttatag gcagttagga
    19501 actgctcatt gtcgtgttgg gtaccaaact tgggtctttt gaaagaccag tttataatct
    19561 taactgctga gtcatctctc cagctccagt ttcaagattg ttggcttgtt tgtttgtttt
    19621 tctagacagg gtttctctgt gtagttctgg ctgtcctaga acttgcttta taccagccta
    19681 gcctccgact cagagatcct cctgtgtctg ggattaaaga tgtgtgccat agtctgcccg
    19741 gtttcagggt ttttaaaaga aaggatgaaa agggagatgg cgcaccattg ggttgcattt
    19801 ggggagttca catacctggc gatatgggcc atgggactgg ggaaagtgac gtgggcacca
    19861 agaataacac aacgttagtg tgcttgcctt cctagatgga ggttgttgcc agaagcctgg
    19921 gctgaaattt tgttagttct gcttttttaa tattatgggg agagtctctt actatcttta
    19981 aaatttcgta gcatttaatc ctagctagtg ctagaaggct acctttaatc ctagctagtg
    20041 ctggaaggct acctttgagc tgacagtact gtttattact aggcaattct ctttctttct
    20101 ttcttttttt aatttatcat aatttagtaa tctagagaat taccttggga agggactcaa
    20161 actgtgttcc ttttttccat ctccatcttt atttttaatt aattaagaga cagggtctcc
    20221 ttatcccagg cttgcctgga acttgaaaaa aaaattgttg tttaaaattt attagatttt
    20281 attgaggggg gaggagctgg tgtgtgtgtg tgtgtgtgcc tagaacacat gtggtgaaca
    20341 ggactgacta cctctgaaag ctctctccac cgtgtgggtc ctagggatca cactggggca
    20401 tcagcatcca gggaagtgcc catcctccct gagccagccc acttgccctg gactttaaag
    20461 aaagagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat
    20521 ggtctgagct cgccatcagt tcacccctac ctagtacccc accacatgct cttttcaaat
    20581 tattttataa cctaccgagt ccatttaggg cttcctgcat gcatatgggc atggggccat
    20641 ctcttcagtc tagatggtac cctctgcact ctgccgccat ctagtggatg cacctttcct
    20701 atgctgggag tggatcacaa agcataggaa ttgtattttg gtggaacaac taaagctagt
    20761 tcctttcccg tcatgggagg gcagcaaagg cccaggtaag tgtaaatgcc gaccaggggt
    20821 ttctttccat cctggctagg agatggcagt gctgagctag ggatccttag caggacccag
    20881 gcttgtcttc ctcacctgtg ctgataaatg caggattcac agggttccct ttgtcagtgt
    20941 ctggacttgg ctttcattca ttctagaccc tttattgcct ccagctcgga gtgccccctg
    21001 gcaatactgt tcagtgttta gttgatgagc gagtgggtcc cctaactgta tctcggtgcc
    21061 tcagccctca gggggctgga aagtcaggta acttctttat gattggtctc aatagttcct
    21121 aaaaagcatt aattgattct tttaatttgt ggctgaatta ttctggttgg tcttttgaag
    21181 gcagagagct agacagatgt agttagattt attcgtgtgt gtgtgtgtgt gtgtgtgtgt
    21241 gtgtgtgtgt gtgtgtggtg ggggtgtgtg tctgtggata tgggcagcat ggcagatgat
    21301 ccaaggacaa tgttcggggg ttgctctctc cttccaccat gtgggtcctg gggatggagc
    21361 gcaggttgtc aggagtagca ggagtaccca ctgatccagc tctttttttt tttttttttt
    21421 ttgagatagt cttgttctgt ggcccgggtt cttcttgaac tcacgatcct ctcctttgcc
    21481 tcctgagagc caggatatag tttactgggg gatccgtgaa gaattcggtt cctgtcctga
    21541 cctagctgtt tacctccggc tctggtgact ccagccctaa gtcattgtta cccactgggc
    21601 tgcacaggcg cctggtccct gggctctcag gcccacagtg cacacctctt gaaccctgca
    21661 ttgtcacatt gctttaaagt tgttaaatcc tgttcctgct ccaccccccc cccacccccc
    21721 gcccccaggt tcctgaaggt agggacctta aggccgatcc caggcctcag tttccctgcc
    21781 tgtacagtga ggagcttggc cttcattgta atctcttaca gctgttaact ttcactaata
    21841 cagttatgat gggcactgtg ctgtggactc cattttggtt cgagttctct ctctctctct
    21901 ctctctctct ctccccccct cccttcctcc ctccctccct ttcccccctc cagttgttac
    21961 tttttattca tattctcatg ggtgactgaa gggagaacat taaagttcaa aataaaatgt
    22021 ccagtgttac agaaccgtcc atctataaga tgcttcgtca gcaccacatc tttaggattc
    22081 ctgtgtcttc cacatgcacc ttatatcagt gcatataatt cccctgtctg ccctgtgcgc
    22141 ggggagacag atagctgtgt atgtgcctgc tttgaacctg aaggaacttg gcctccgcaa
    22201 aatttcagtt ggctttagtc cttggtacta agtgactgag tgggcactta ataatgaatg
    22261 tttggatcct aagctctgat gttcattgct ctctattttc aaggactttt gagtaatgat
    22321 tttatatata tatatatgtg tatatgaatg attatatatg tatgtttatg tgtacacaca
    22381 tacttagagg attgtgtttg ttaaaatatt aaacttgctt aaatgggaaa ggttacgagt
    22441 ggtttataag aagggataaa gacagataac ccaggtgttt ctgcaaaggc ggcatcatta
    22501 ccgtggtaca ggagagttgt aggagcatgc agcttgggag agctgaggtg acctcacaca
    22561 gcacctgggt cctctgagtg tgtgtgcgcc ccacccacaa cagtggtcat tggtcacatg
    22621 attaatgttg cttggtggac ttctgaaaag ggactcacca accaaggaac taagttttaa
    22681 tttccttaat ttatttatat tttattttta tcatacagac tcttctgaat ccttagctag
    22741 ccttgaactc aatccatagt taagttctga ccctcctgcc tccccaaggc ctgggactgc
    22801 ggacgtgagc cagcatgcct gacactgtgg gctctgggtg ttgtgatgct acagcaccct
    22861 gccacctgtc cccgcagccc tgcctgcccg tgatttaagg cggttttaac cagagttaaa
    22921 tacatgtgtg tagctgctgg gtactgtgtt ggacagaata gctgtggata aaattcccaa
    22981 gctaccaagt tgtagattta tcatttctaa aggagaggat tggcttagtt gctttctgga
    23041 gtgtccttca ttttagaagt tatgatctgt gccttgtaat attagagtga ttcaacagtg
    23101 ctctgctctc gggagttgag cacagttgtc tctgatttat atcaccctag ttccagctct
    23161 gattgggtgg tcaggatcca gggaagggca gcatttgatc gtatctgtcc aataataatg
    23221 acagtctaga gaaggttctg ggaaagagcc cacagtccca ggagctgttg ttattttcat
    23281 ctatagaatg aaatatagaa aatgtcattt tcattatttc ttgtattttt ccttgctttc
    23341 tattagggtg attagaatag gtgggtcaga tggtactgtt tcttcctggg ggacagactg
    23401 gggaccccct cacctctctc agtaggtgtc ttccattctg cgtgtttcag tgacatcagg
    23461 gaggagattt tgaggcagtt tttgatagca gcctttattc tgcttgtaga tataaaagca
    23521 ttcttgtgag ttgctcactg gtccattgtg ccagcatttc agaaacccca aggcctctgt
    23581 ttgagcactt gccaagtttg tcctcactgg agaagacaaa acagggtttt ctggaaacta
    23641 ccaccaaaga cccgttcttt cctgagcttt tcctttctgg tggactggcc ttggtgtgta
    23701 agggtcacat gttttagtat cagaatccac cctgctctta gcagcaacct ggccccactg
    23761 caggcattgc tcaccttacc caaagtaact tatgagaagt gagaactaaa ttacctcttc
    23821 acagacctga ctctttctga ggtttctgag aagttttatc cacgctcttt agtctgtcct
    23881 caaactggac attagtgctt tgaggaatgt gagggccagg aaggtgagtg gctggtggag
    23941 agttaggatt ggtaagtcaa gagctggctg ccagtctcca gcacctcaac ggcctttttt
    24001 tccttttctt tttgtgagct tggaatttct gtgtgagcat agtccttcta gaggctactc
    24061 tcatacaacg cttgtgaaac attggccatg ttctggttat tgtagcctgg gatgtaacac
    24121 tgagatgcac ttaggagtgc ggcagctgtc atgtgggttg agagtttaga tggaaggatt
    24181 tgggacatga cacacctttg tgcttctttc aggctcactc tttgacgtcc cctggatcag
    24241 cattcttgag ctggcagctt gttgcacatg gcttcttcag attcgtgctt gactacgagt
    24301 ctcactggtt gtgaggtggc acgtccagag aactcccttc gctctatcgg accaactcca
    24361 cagtgtacgg ctgttaacac agaatctgcc tcccctcact gtagccctta ctttccatat
    24421 taaaaacatt tcagcctcca agtgccattc gttccttcat gtttgtgtgt ccgtttccct
    24481 ccgctcagtg cgctctcatg acttgctccc agctgtgact cttcacctgt gacccttcac
    24541 ctttgttttc attaagtagt tgtcattgct gacaa
//