LOCUS       KO-first_condition_ready_27523_MGI:101903        22956 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Klra5 targeting project(s): 27523
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 106336
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            8915..9004
                     /db_xref="ENSEMBL:ENSMUSE00001284647"
                     /rank="4"
                     /note="ENSMUSE00001284647"
     exon            7382..7516
                     /db_xref="ENSEMBL:ENSMUSE00000473326"
                     /rank="3"
                     /note="ENSMUSE00000473326"
     exon            5653..5726
                     /db_xref="ENSEMBL:ENSMUSE00000715055"
                     /rank="2"
                     /note="ENSMUSE00000715055"
     misc_feature    4215..9386
                     /note="5 arm"
     misc_feature    9414..9438
                     /note="B1 Gateway"
     misc_feature    9451..9498
                     /note="Frt"
     intron          9499..10373
                     /note="mouse En2 intron"
     misc_feature    10371..10373
                     /note="Splice Acceptor"
     exon            10374..10546
                     /note="mouse En2 exon"
     misc_feature    10558..11151
                     /note="ECMV IRES"
     gene            11152..14212
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14251..14494
                     /note="SV40 polyadenylation site"
     misc_feature    14498..14531
                     /note="loxP"
     promoter        14558..15077
                     /note="human beta actin promoter"
     gene            15103..15897
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16097..16338
                     /note="SV40 polyadenylation site"
     misc_feature    16355..16402
                     /note="Frt"
     misc_feature    16409..16442
                     /note="loxP"
     misc_feature    10388..10465
                     /note="FCHK std_gpr"
     misc_feature    16458..16478
                     /note="B2 Gateway"
     misc_feature    9387..16508
                     /note="Synthetic Cassette"
     exon            19117..19256
                     /db_xref="ENSEMBL:ENSMUSE00001056751"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00001056751"
     exon            16866..17084
                     /db_xref="ENSEMBL:ENSMUSE00001288414"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00001288414"
     misc_feature    16509..19433
                     /note="Critical Region"
     misc_feature    19457..19490
                     /note="Downstream LoxP"
     misc_feature    19434..19513
                     /note="synthetic loxP region"
     exon            22232..22335
                     /db_xref="ENSEMBL:ENSMUSE00001075310"
                     /rank="7"
                     /note="ENSMUSE00001075310"
     misc_feature    19514..22956
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaggaggc tgcagtaaga taggagaaac ttttctatag gatatctgag
     4261 gatattctta gccttcatgt tggtggaagg tagtggtctg gtaggttcat agatttcatg
     4321 aagtttttat ctgccagtga caggatgttt gttctgacac aggttattta tagggctaaa
     4381 cctattaact ggtaagataa ttacttgtta gatccacaaa acaacaaagt tcacccttct
     4441 ctggactttc aatctgtctt tgctgaaaca tcttctttcc aacaattttc atcacaaacc
     4501 aataggaaaa actcatagaa gaaaactcac ctcatctact ctctaaaaag tcaactgtga
     4561 gaattttcac tatttaccaa ttgaatgaaa ataaaggaga tgtcatctga tatcctataa
     4621 tagagttctt taaatgtata tctccttgag tcagatgact tcatgcataa aatgtcttgc
     4681 ttagtatgtt ctaagaatat ctcatatagt ttttaaacta aaaatagcaa aatagtataa
     4741 aacaactagg atattcagta gcattaggtt agctaattaa tttatgctgt acataagtaa
     4801 ttagtaatta tacattactt taaatatata tatatatata tatatatata tatatatata
     4861 tatatatata tatgaaatgc ttgttcttta tttattaacc tggactaaat tggtgtcatg
     4921 gtatacagct ataattctaa aacagaaaag agtgagaact gaggaatgtg agtgtaaggt
     4981 cagcctatgc tacatagtaa cataaaacat ctcaaacaat aacaataaca aacaaaacaa
     5041 aataaaacaa attaaaaaaa aacaccagaa caaaacaaaa ccaagtcctg agtctgtagc
     5101 tcagggaaag aatatttgct gagcattttc attgtcttag atttaatttt cagcaccatg
     5161 acaacataag catcaaaaca ctccatacag aatgacatta atgttcaatg gaaatattat
     5221 ctatgattga agatctagaa aattaaaaaa aaagacttca gcagtgtaaa tgtgagtgct
     5281 taaaagcatt cttaatatat acttagctgc aattagcata attcaaaatc aaggagtaac
     5341 taaaaaatat agtaacataa gaataaattt caaacagtct atgacaaaat tagcctacaa
     5401 acttcagtcc ctctttttcc tatttgtttt gctaaaggca gtgttttgcc ttctctgtag
     5461 gagtgtctat cttctctgtc ttaaaatata aattattcac atttgttttg tccacctgat
     5521 actatatgtt gtttcaaatt gcaataagca atttcctcct tttgcttaga taaagaggag
     5581 gggcagaaaa tcatgaggtt gagtatcact aggtggaaat ttagttccgt ctttcatttt
     5641 tgaaactcgt aggggatata gaccagaaaa cgccaacttt tcacccaact tttccctcca
     5701 ccagaatcac tccggtagag acacaggtaa aaataactgc ttttttctac taaatgatca
     5761 aatataatca agagaatttc aaggggtgtg atatgagatc tggattgatg gggaagcaca
     5821 ctgggttgtg ttagatctgg ttaactctgg cagcatgtga caacggttat tctgcactaa
     5881 ttgttttctt aggagtatac tgtgacttgt gtgctaagaa attaaagtag agggatctct
     5941 aaagttcatt actctataac tgcatcaaat gtttatatat tatccatctg aaagatgtaa
     6001 taataggatc caggtaaaac gggtcagtgt gtaaatgtac ttgctacata aggctgactg
     6061 accacctagt ttgatccctg gacttcacat gaatgtggag tgggaggatt cacttagtaa
     6121 aattgtcttc tggacatgtg tacctcagtt tacccagcca aagagcaatg ataataaata
     6181 agaattttga aaaagaattc tatgacagaa tgattctttg tcaaagcaaa ttgtgaatca
     6241 attctgaaaa cttaataaat tgaaaaacta gttaagaatg ttctcacatg tatatcttgt
     6301 atttgttatc tgctggccct cagcttagtt attaatgtaa gggtaatgtc agttataaat
     6361 actatttaca aatacattga tgtctattat cagatggtat ctatgtactt tagctaatat
     6421 gtactgcata gaggcaaaat ttattttcat atagcctcct cttacaacat catgtgctta
     6481 tttattatat gtgacatgat catgtacatg gaattatgtg agattaaaaa ttcacattgt
     6541 atcctctata cctctctctc tgttgctcct acaccctagc tggcaatggc cctttatgac
     6601 ttttctgttt gctgcagtta ctaaaagtta caaagcattt gtgatgactt tgatccagga
     6661 gtctcagata agatagacta taccacattt ttcttttttc cctactcatt aggaacttat
     6721 ctgtttgaat tcacttacca ggtccaacca tggataagga tgtatagaat atggctaggt
     6781 gggcaggttg gacatctcag ctgattgggg cttcaggcca ataaatattt atgtgccttg
     6841 gatcacctgc agtattaatc ttcaaatatc ttattgggaa attctgagag tcatgtataa
     6901 ataaaaaaac tgaaaaaaca attatattat gtattatcaa aatatataaa taattctaaa
     6961 acaacatttt tatttattct taaattttca actctttgtt ctttgaactg attgtttaaa
     7021 tgtatgtaaa tcatttttag tggtaccaat tttacctaat atttctcttg ctaaatattt
     7081 tgaacaaata ggctgtatca atggtagaat ggcatagtaa atttttaatt ttttttcata
     7141 tttcttagga ataattgctg ttaccattag aaatttaaac aactttagca gaaatctaca
     7201 atgagttctc acatgtaatg gaaatcggga tcaatctatg tcagggcgtt tggattatta
     7261 agtgagctag tcagactcta catttttcca accctctgtc tcatcagctt cagttatctt
     7321 cctataggtg aacactttaa catttttctt agaattcact caaaggccca ttttaccaca
     7381 gaacatactt catgcactcc cacgatgagt gaaccagagg tcacttactc aactgtgaga
     7441 cttcataagt cttcagggtt gcagagacta gtgagccatg aggagattca agggcccgga
     7501 gaagctggct acagaagtaa gtgtttaaag tatctaatat tacatatatt ttaaaatgca
     7561 ctttaaactt gaaaaaaata gaaatattct agttaaaact ataatgatcc atctcaccgg
     7621 ggcttggtta tgcacaagga tgggagggaa gaaaagagta ttagttggat ggtagtcatg
     7681 tacaaaatta gtcaaatcta agttttaaaa agaaagcaag tccagtttta aattttaaaa
     7741 aagaaagaat aatttataac ccaacttcta cactttagtt ataactgact acaaaagaaa
     7801 taaaattggc agaaaataat tatggaaata tataagaaaa aattttcttg ctaatcaaga
     7861 agaaatgaaa aatcattagg aggtttttca ttattcatac tagtcaggaa gtattttgaa
     7921 aaaaataaat aaatctaaca gacaaactag agtttgaaag tacaccaaat gttctagaga
     7981 aaaaaatcct aaggaaacac agatttttca ttttttcctt ctcaaaagaa cttcttaaaa
     8041 atatatctaa cacaacatct aacacaacag gcaataacag ttgactatca gaacaagaaa
     8101 tctcctactg gaaatagttt cagatgaaaa aggaagagat gacaggatag tgggaataga
     8161 aatgaataaa ccccagtaac agttccagaa aatgttgaag aacaatttca tttttaggga
     8221 aaagcagaaa tctcactttt taattttttt ttctcatttt acactgtacc atggtgcttc
     8281 aagctgagat ctttatcttt ttcgatgtag tggtaacaaa aggcatgagt ttcagattcc
     8341 aaatgcctaa gataaaccaa aatgtttagt attacagaga gcacaatcaa acttacaggg
     8401 acaaagagat agttgactta taggcccttt caagaatcgg gatacataca ggtcaagagg
     8461 atctgtggtt ccctaccagt tctgaagaac atacatcact ctttcttcct ctccattctc
     8521 atttacattt cttggtggac tgcatgctcc agtttggcat cttgtactga gattgtatct
     8581 atgccttcag tgccttctat agaatttaat tttctttgtg tagaactggg tcagggcaca
     8641 tgcctgttat ccagctcatt ctaggctgag tcaggagaat gcacaataaa aggacaaggc
     8701 agaaataagt tcaaattttt gtggaaattc acacacattg gatttctaca atgggtgaat
     8761 tttaggtaga aatgaaaatg tcgagagcat attttcagtt tgtttgtatc attcacttgt
     8821 gaaaattttt cgccttgttt ccatgctaca aaaaattaaa attagtcctg agatatgatc
     8881 ttttcatgat tttcctgctt tttttaaatt taagagtgtt cagtaccctg gcagctcact
     8941 gtgagatcgc ttggaatctt ctgtttcctt cttctagtaa cagttgcagt gttggcagta
     9001 aagagtgagt aattgagaga tatgttgctt ttccttctgc ctacagcttt agacatgaac
     9061 tatgcagtta tacacactgt ccatggttct gcctaaaatc aggggataag ccaaaaatct
     9121 ttggtgttat aatgtttttt acccacaaag tggagtgttc tgttctctat catactttat
     9181 ctgttggctc tggggctgtt gagagttccc ctgttctgtc acttttggtc atgttccttc
     9241 tgaggactgg gtggtagtct actttctgag ctcaggggat tgtaaatcta atgtaaacat
     9301 tttttgaaaa acacctatgc aaagaatcga tgaccttcct tttatagttt tttcaatgta
     9361 tactgaagtc ttgcttaagt aaaaacaagg cgcataacga taccacgata tcaacaagtt
     9421 tgtacaaaaa agcaggctgg cgccggaacc gaagttccta ttccgaagtt cctattctct
     9481 agaaagtata ggaacttcga accctttccc acaccaccct ccacacttgc cccaaacact
     9541 gccaactatg taggaggaag gggttgggac taacagaaga acccgttgtg gggaagctgt
     9601 tgggagggtc actttatgtt cttgcccaag gtcagttggg tggcctgctt ctgatgaggt
     9661 ggtcccaagg tctggggtag aaggtgagag ggacaggcca ccaaggtcag cccccccccc
     9721 ctatcccata ggagccaggt ccctctcctg gacaggaaga ctgaagggga gatgccagag
     9781 actcagtgaa gcctggggta ccctattgga gtccttcaag gaaacaaact tggcctcacc
     9841 aggcctcagc cttggctcct cctgggaact ctactgccct tgggatcccc ttgtagttgt
     9901 gggttacata ggaaggggga cgggattccc cttgactggc tagcctactc ttttcttcag
     9961 tcttctccat ctcctctcac ctgtctctcg accctttccc taggatagac ttggaaaaag
    10021 ataaggggag aaaacaaatg caaacgaggc cagaaagatt ttggctgggc attccttccg
    10081 ctagctttta ttgggatccc ctagtttgtg ataggccttt tagctacatc tgccaatcca
    10141 tctcattttc acacacacac acaccacttt ccttctggtc agtgggcaca tgtccagcct
    10201 caagtttata tcaccacccc caatgcccaa cacttgtatg gccttgggcg ggtcatcccc
    10261 ccccccaccc ccagtatctg caacctcaag ctagcttggg tgcgttggtt gtggataagt
    10321 agctagactc cagcaaccag taacctctgc cctttctcct ccatgacaac caggtcccag
    10381 gtcccgaaaa ccaaagaaga agaaccctaa caaagaggac aagcggcctc gcacagcctt
    10441 cactgctgag cagctccaga ggctcaaggc tgagtttcag accaacaggt acctgacaga
    10501 gcagcggcgc cagagtctgg cacaggagct cggtacccgg aagatctgga ctctagagaa
    10561 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
    10621 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
    10681 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
    10741 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
    10801 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
    10861 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
    10921 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
    10981 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
    11041 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
    11101 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac catggaagat
    11161 cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    11221 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    11281 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
    11341 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
    11401 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
    11461 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
    11521 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
    11581 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
    11641 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
    11701 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
    11761 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
    11821 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
    11881 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
    11941 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
    12001 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
    12061 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
    12121 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
    12181 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
    12241 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
    12301 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
    12361 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
    12421 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
    12481 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
    12541 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
    12601 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
    12661 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
    12721 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
    12781 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
    12841 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
    12901 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
    12961 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
    13021 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
    13081 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
    13141 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
    13201 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
    13261 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
    13321 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
    13381 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
    13441 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
    13501 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
    13561 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
    13621 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
    13681 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
    13741 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
    13801 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
    13861 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
    13921 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
    13981 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
    14041 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
    14101 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
    14161 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
    14221 ggcagggggg atctaagctc tagataagta atgatcataa tcagccatat cacatctgta
    14281 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    14341 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    14401 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    14461 aaactcatca atgtatctta tcatgtctgg atccggaata acttcgtata gcatacatta
    14521 tacgaagtta tgtttaaacg gcgcgccccg gaattcgcct tctgcaggag cgtacagaac
    14581 ccagggccct ggcacccgtg cagaccctgg cccaccccac ctgggcgctc agtgcccaag
    14641 agatgtccac acctaggatg tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg
    14701 gggcaggcct ggccatgcgg ggccgaaccg ggcactgccc agcgtgggcg cgggggccac
    14761 ggcgcgcgcc cccagccccc gggcccagca ccccaaggcg gccaacgcca aaactctccc
    14821 tcctcctctt cctcaatctc gctctcgctc tttttttttt tcgcaaaagg aggggagagg
    14881 gggtaaaaaa atgctgcact gtgcggcgaa gccggtgagt gagcggcgcg gggccaatca
    14941 gcgtgcgccg ttccgaaagt tgccttttat ggctcgagcg gccgcggcgg cgccctataa
    15001 aacccagcgg cgcgacgcgc caccaccgcc gagaccgcgt ccgccccgcg agcacagagc
    15061 ctcgcctttg ccgatcctct agagtcgaga tccgccgcca ccatgattga acaagatgga
    15121 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
    15181 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
    15241 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
    15301 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
    15361 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
    15421 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
    15481 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
    15541 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
    15601 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
    15661 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
    15721 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
    15781 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
    15841 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
    15901 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
    15961 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    16021 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc ccggatctaa
    16081 gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt
    16141 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    16201 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    16261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    16321 cttatcatgt ctggatccgg gggtaccgcg tcgagaagtt cctattccga agttcctatt
    16381 ctctagaaag tataggaact tcgtcgagat aacttcgtat agcatacatt atacgaagtt
    16441 atgtcgagat atctagaccc agctttcttg tacaaagtgg ttgatatctc tatagtcgca
    16501 gtaggcggat tgtgccattt cttaacatgt tttttagttc acattttcat agtgtgttaa
    16561 gtattcactg aatattttac ttaagtaatt atctgtaaat ttttgctgta aaattgaagt
    16621 gaatggaagt tacttctacc ataattttat tcagctgtat tatttatttt acagtaaact
    16681 taatttagag aaaaatgaag tatctgtttc tatagtgagt taattgaagt gctaatttat
    16741 gaatgttaaa attccaatga tattcatgat atgtgttggc tatccacatg aagctatttc
    16801 tctaccttaa attttgatat gtatttctta taagcattta gattttatat ctgtgattaa
    16861 tttagttttt cagtatagtc aacacaaaca agaaatccac gaaactctaa accacaacca
    16921 taactgcagc aacatgcaaa gtgacatcaa attaaaggaa gaaatgttga gaaataagtc
    16981 tatagattgc agtccaggtg aggaacttct ggaatccctc aacagagaac agaacagatg
    17041 gtacagtgaa accaagacag atttagattc ctcacaagac acaggtatgt agcagaggaa
    17101 aaccatgagt acagaattgc attattatat attatttctt ctctggaagc tttgacgtac
    17161 atccagcagc tgaagatgct tgtgatcttt gggttaatca tttggggggt cacttacttc
    17221 atgtagagat atgaaaaatc cgaaatccat atttggatga ggagaatctt agagtttcta
    17281 gaagccaaat tctgtttggc actcctactg caccctggag agggaattac aaattgatac
    17341 atttgatact tttgattttt ttttctgtct agcagaaaat ctgagtctca aactttgctc
    17401 ttgacagaat gtggaaggtt ttaataagag tgcatttatg aactttttat gctaaaatgg
    17461 acttagtgca tttacatgga tgcagttata agaagaattt ttgtttgtgc atgtgtgttt
    17521 cccatctgac aggttttgaa agatctgtac tatttgtaaa taggaccctc tctgggacag
    17581 acaagggagt ttaaccatgg tgtttaaatg ttgcaactct gccagtgaca cagtgaatga
    17641 atgaggctgg gaaaatgaaa aaatcaagag tcacaaggaa cctttcttgc ctatgccagt
    17701 gactggtcat ttttactgac acagtttatc aattatcatt aactactttt tcaagatttg
    17761 taacaaagag ctcttgtata taatctcacc actctgtgac aataggatgt gatgattctc
    17821 attatgtcag gattaatata tttaaaactg ttacattata aaatttgtga taggaaaaaa
    17881 tagctccaaa acaatctttg aagtattcca gagccatgga aaaacttttg tgccattact
    17941 ttaaaataac ttaattatct gtataggaca gcacaatgat gtaatcccta tgactgtgag
    18001 attgagggag gagtattata acattcagta atataaggac atcccagaaa tttcaagtcc
    18061 agcctgagct acacattgat tcctcttcac agaaaagtaa tggttagaaa taaacttacc
    18121 aaatagatca tggacattcc cagacactat ctaaacaaaa cattcatatt gagaggatct
    18181 tgcgaaacca actgcacaca ttttaatgta ttcaacttta aagatttggg catgtgggca
    18241 caacactggt ggaatcaaca taatcaggag aacagacttt caacaccaaa acctttctca
    18301 tttggctctt atttctctta aagtaaaaga atttaacaca gatttcggca caatgctaac
    18361 catatacaag tgtgtggatg agtgtgggca caacgcagta ctgcatatct agaattctgt
    18421 cagcagttta actgcctctg agtagcaagt gctcattatc tgcctctagt ccctggtatc
    18481 ttgcaagcct ctgtttcaca gaacttgttt tttttttttt tcaaaaaaaa aaaaaacaga
    18541 tctctatgga atcatattgt gtggccctat gcccagtgtg tttcactttg caaaccatcc
    18601 tcaagttcag tgatgtttgt cacataggtc agcatcttgc ttttggattt gctgtggaac
    18661 aagatgtcat ctgaatccca ggatgactgg gaactcacaa tgtagagaag actgacatga
    18721 aagtcattgt ttagccaagg aaggcctcaa actgttagtg atcttcctgt ctttctcttt
    18781 agagttctca tgtcatagac ataaatcatc ctgccaagct gattcatttt cctttaaagg
    18841 ataaactata tttttgtgtg tatgtgcttc attttctcta atgattcatc catgattgac
    18901 acaggaaata tttttagatc tctagtggta ttaaccatgt tgcatagatc atgcatgagt
    18961 atgcaggggt gtttggccat tatagaattc tactttctga gacctatgag attttacctc
    19021 ttttttttaa aaaaaaaaca gatttaataa atatattcca ctgataacac aataccagaa
    19081 aataataagt ttagacattt accttctctg ttgtagggac aggtgttaaa cactggttct
    19141 gctatggtac taaatgtttt tatttcatca tgagcaaaaa tacatggagt ggatgtaaac
    19201 agacctgcca gcattacagc ttaccccttg tgaagataga agatgaagat gaactggtac
    19261 tgtagaggct cacttgcccc ttattttctt agactctggc tctaaacctg aataatgagg
    19321 tactggatcg attttttgca tttgtatatg attttcctgt aatgtgaaca taactgtggg
    19381 acacaagtct gttaagtgga aactcattat tacaaagaca cttgtgtctt tcagagatgg
    19441 cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat ggtctgagct
    19501 cgccatcagt tcagtacaat ctgttggtat agcatatcct gttaactgag agtcatttat
    19561 ctccatcgag ggccaagtaa acccctcttt tttaggattt gctctttgga acctttaagt
    19621 gagtctgcct gagatgagca atcagatgac aacagggaaa gcagttgtgc tctggtcctg
    19681 tcttcaaaat gagatgatat gagaaatgtg gaattaaatg gttgaccagt tagctccata
    19741 gtgattgcca ctgaaattgt tcacaatact tactgtatag tattcctatg tcctgtaaaa
    19801 aggatatcca tctttagaaa tgactattaa aaaaaagttt tcaagattct gtcatttcct
    19861 cttttaatta ttacttatga aatcttttca aatatgaaga ctgtattttt ctagagaaaa
    19921 aattacagga tcaccttgaa aactttgtta tttataacat ggtatgatat ttatacatca
    19981 atttaataga tttttaaata acattctaaa aggatatttg atgccaaaca atagtgtaat
    20041 gttccttttg atacagtaaa aaaaaagttt ctaaaattta ttattattat tattattagc
    20101 tgccttagta acagtagtat ttgtagttgt agtcatagtt gtagtcgtat ttatattcat
    20161 cgtagtagta ttttatttat tcattttaaa tcctgctcac tgccccctct tggttacccc
    20221 ttcaataatc catccctaac catccttctc ctctggttgg atgggtgcac cctaggtatc
    20281 accacaacct ggcccaatac atttccataa gaataggtga atgtgctccc actgaaacta
    20341 gacaaggcag cccagttaca agaacttaac ccatgtacag gcaatagctt ttgggatagt
    20401 gcctactcca cttgttcaga acatacactg aaggccaagc tgcacatatg cagggaggct
    20461 gaggtccaac ctcacgtatg ctctttgttt gtggttccat atctgagagt cccaagagtc
    20521 ttgattagtt gattctcttg gtcttactat ggagatccta tccctttcat agcccataat
    20581 ccttccacct gttcttccat agatgtcctc aagctccatc cactgtttag ctatgggtgt
    20641 ttgtatatgt atgattcaga ctgtggacca agactcacag aggacatcta tgctagattc
    20701 ctgtttgcaa gcataactgt gtatcatttg ttgggtcaag gattgatgat ttttcatgga
    20761 atgtgactca agttaagttg gttattggtt ggttattccc ttagactttg ctccaaccac
    20821 aatccctgtg tttcttatag atgggataag tttagggaca aagttttgtg gttatattgg
    20881 tggccctgtc actcctccag gattctttat aggccacagg aggcagcctc tccaggttac
    20941 atagagatac agctaaggat tccctggaca attcttgtgt attttcccta tgactggtat
    21001 ctatctcttc tgggagatgt ccaaaaccaa cccccccatc agttgcagat ttccattcat
    21061 tctcatgccc atcaggccat tcctcctccc ctattcacaa cagattatta accctgacta
    21121 atctcttgat ctaatttttc actcagttcc ctccctctat ctaccttcta tgattattcc
    21181 atttctgctt ccaagtggga ctcaagcatt cttgcttgtg catttctttt atccagccac
    21241 tggggtctgt gaagggtatc atggacacca gaattttatg tataatatca actggtacgt
    21301 gaagacatac cctgaatgcc atttttggat ttggttacct cagttatggt gacattctta
    21361 cgttccataa acatgcctgc aaaattcgtg atttttttcc atagctgaat agtactccat
    21421 tgtatagatg tagcacattt tctttatcca tttttcagtg aagggacatc taagatattt
    21481 ccaatttcta ggtattatga ataaatctgc tatgaacata tctgacaatg agtctttttg
    21541 ggatggtgga gaatccttga gtatatgccc tggagtggca taactggttc ttgaggtaga
    21601 accagtatcc tgagaaacaa ccaaattgat ttctacaagt gtacccccca tcagaagtgt
    21661 agtggtgttc ccctttctcc atatccttgt catcatgtgc tatcacttga ctttttaaac
    21721 ttagccattc ctattaggga aagacatgat cttagttttg ttttaattgg catttctata
    21781 gtggataaga gccttgaaca tttattaatg tgcttcttgg tcattcgaga ttcctctgtt
    21841 gagaattctc tgcttagctc actcatagta gatgcaatag aatgaattga tacaacagtc
    21901 aaaaaatgac aaacctaaaa catgccagcc ttaagtagga agattttgag ttcaaggatc
    21961 tcttggttaa gttcagagac ttggtataag aaaaaaaata acaaatcctg tcataaactg
    22021 aggattcttg aagtttttat ttaaatagct tattgaattg acattaattt gatataaata
    22081 ggaaatcagt ttagaaaatg aatatggtgg gactgagtgg tgggaggaca ggacatcaga
    22141 gaatgacata gtgatgctgg gaacttttcc tgcttgttct ctcatccaac agatagaaat
    22201 ttcatcataa gcaatgttgt ttcctttaca gaaattcctt cagttccagg ttatttcaga
    22261 cagttactgg attggattgt catatgataa gaaaaaaaag caatgggcat ggattgacaa
    22321 tggcccatct aaactgtgag tttctgatcc cttcagactc taaccctccc ttggggattg
    22381 agagggttgt ctgcaaaggc ttttctccat ttctcatggt tttcctcatt ttctatggag
    22441 ctcagaagta aggacaagtt gtataaggca gttcctgata aagtgttacc aaaagaagat
    22501 gtatagtcaa ggagatgacc aaaagtgact tagtattgca cacatctaag gatggtgtgt
    22561 tctaacatgg gaagggcact agaaagtcaa taacttaaga tagtttattt atttattata
    22621 tgtaagtaca ctgtagctgt cttcagacac accagaagag ggagtcagat ctcattacgg
    22681 atggttgtga gccaccatgt ggttgctggg attgaactca ggacctttgg aagaacagtc
    22741 gggtgctctt acctgctgag ccatctcacc agcccttaag aaggtttaaa gttagggaat
    22801 ggtcaagaca gatacaatga gtctgagtat aacagatatt catttgactt tttattgttg
    22861 ttagctttta ttattgccag ttatttataa atataagagc atttttatgt atacatacac
    22921 atacattcca tttaaaggtg ttatgtgtaa atctta
//