LOCUS       KO-first_condition_ready_39556_MGI:101899        20387 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Pla2g5 targeting project(s): 39556
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt1
COMMENT     design_id: 39187
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            7941..7990
                     /db_xref="ENSEMBL:ENSMUSE00001271923"
                     /rank="3"
                     /note="ENSMUSE00001271923"
     misc_feature    4112..9099
                     /note="5 arm"
     misc_feature    9127..9151
                     /note="B1 Gateway"
     misc_feature    9164..9211
                     /note="Frt"
     intron          9212..10086
                     /note="mouse En2 intron"
     misc_feature    10084..10086
                     /note="Splice Acceptor"
     exon            10087..10125
                     /note="mouse En2 exon"
     misc_feature    10126..10142
                     /note="Frame 1 insert"
     misc_feature    10143..10196
                     /note="'self-cleaving' 2A peptide"
     gene            10197..13274
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13275..13328
                     /note="'self-cleaving' 2A peptide"
     gene            13329..14120
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14319..14561
                     /note="SV40 polyadenylation site"
     misc_feature    14578..14625
                     /note="Frt"
     misc_feature    14632..14665
                     /note="loxP"
     misc_feature    10101..10216
                     /note="FCHK std_gt1"
     misc_feature    10101..10118
                     /note="FCHK QC prefix region"
     misc_feature    10119..10198
                     /note="FCHK QC critical region"
     misc_feature    10199..10216
                     /note="FCHK QC suffix region"
     misc_feature    14681..14701
                     /note="B2 Gateway"
     misc_feature    9100..14731
                     /note="Synthetic Cassette"
     exon            15151..15295
                     /db_xref="ENSEMBL:ENSMUSE00000181793"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000181793"
     misc_feature    14732..15465
                     /note="Critical Region"
     misc_feature    15489..15522
                     /note="Downstream LoxP"
     misc_feature    15466..15545
                     /note="synthetic loxP region"
     exon            19125..20387
                     /db_xref="ENSEMBL:ENSMUSE00000661792"
                     /rank="6"
                     /note="ENSMUSE00000661792 5' fragment"
     exon            18328..18434
                     /db_xref="ENSEMBL:ENSMUSE00001228294"
                     /rank="5"
                     /note="ENSMUSE00001228294"
     misc_feature    15546..20387
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg actacaggct cctgagaaat ttggggtcgg
     4141 caagttagaa aggagagaaa gaagggctgc cctcgagctg aggccgaaga agacagaaca
     4201 aaatagaagt ggttctgtgg gctgggaaac cacaaggaac caagggctgg atcattccac
     4261 aaaggcccac tgtgggagcg gaagtgaggc tgtgcctggg aggggatttc agggctctgt
     4321 ctctggatgg ggactagtct cagacccacg tcagctcaaa gcagccactt ccagatatct
     4381 cccctgtgag cgccccagct ctgagggctg tatcaagaat ctagagaagg agaaaccaca
     4441 gggggtcttg aagtgggcct ccctgggtcc ccaggaatct tggaggcggc ccacgcagca
     4501 gtgtgggagg cgacaggact ggtttaaaac caagcagacc cacctgccgc ctcttctccg
     4561 gaactcagga cagagactag caatcaggtg agactctcat tatgggactg cccaatgggg
     4621 gcggctgggg gccctgtccc tctgtcggga agcagccagc acagcgggca gatatctcaa
     4681 agctgagtgg accattggac tctttcccag gcaagagatg aaaatggagg tccaaggcct
     4741 acagggctcc cgcaggtggc cttgaaggac ccgttcctca cgttgatctt ggcagctcca
     4801 tcacttccac atcaccctcc attctcccgt cttgtccata tcggctctcc tcacctttca
     4861 tcatcgtagc ctggctccac cctgaaatta tctcgcagtg tccctgtgcc cctgtggtca
     4921 tcggataaaa acagacaagc cataaccttg tctcagggct gccttggggc tgcagagatg
     4981 ctctggactc ctgcacagcc gtttccaggc ccagccactg ccgacacctc cctggaattt
     5041 gtcttatctt tgcaaatcct tttataagga ctcctgcagt acccacgcag caacagccca
     5101 gcccagggag gtctgggtga ggacagtcca caggagaaga gagttggctg tcacgggtgt
     5161 gagtctgcag ggaaagagag caggatgggc taactagcga gattcacctc agtagctcta
     5221 ggtttcctgt cagaggccat tgggctaaca ttgactggcc tggcctgaaa gggttttaag
     5281 tatgtgggtg ctgggatttt gaaggttttg actggtctga ctggtatgta aaaatcatac
     5341 ctgcaggaga gccaggtctt agctggagga actggctagc ctacaggggg cacattctct
     5401 ttatcaagac ccttgagatg tcaaagcgtt atgaaataga atacccaggc agggaatgta
     5461 gctcagttgg cagagcactc gcctcgcgtg cagtaggctc tggggtccac ccagcaccgt
     5521 acaaattggg catggtggtg catgcctgta atcccggctc tttcttgagg ggtggaggca
     5581 ggaggatcac gaatttgact acatagttca aggtcagtct gggctccatg acaccctgtc
     5641 taaatgaaaa ggaaggaaga gaaaaaagaa agaaaggaag gaaggaaaga tgagaggaaa
     5701 agaacaagga aggaaaaaaa ggcaaaaaga aaacaggaaa agaggagtgg aggtcgccat
     5761 agagagatgg aggagaatgc tcaggacagg attctctagc ttgggctaaa cctaaacctt
     5821 tgggcaactg tttcacctac atggggcttg ttcatcctct cctctcctct cctctcctct
     5881 cctctcctct cctctcctct cctctcctct cctctcctct cctctcctct cctctcctct
     5941 cctctcctct cctctccttt cctctcccct tccctcccct aacccaatct cccctccctc
     6001 cccgcctcat atatatatat acacatatgc atatataatg tatatatata tatatacata
     6061 tatatatata tatatgtaca gaagttgaat ctggagagtc tggagtcagg ctgacagcca
     6121 gccagtccca gtgaaccccc tgtctgtgcc ccacaccact ggggttagag gcagaggcaa
     6181 atacccactc agcttgctta tgtggccact agaaggcaaa ctcagattct caggcttgca
     6241 cagtgagggt ttttaactca ccaaagctac ctccccagcc cctggcctga gcctgacagt
     6301 tcctcagtct gtaaaatcaa taactcttat ccattcatcc attcatctga cacagatccc
     6361 cattgaaggg gacatacagt ggcttgccag tgtgtaccca tcaaggccat gagtggtttg
     6421 agagtgacgt ggatggtgtc tggcatcctg aagataccca gtgtgtagtc tctggtgtct
     6481 ctgcttcaaa aaagcaaaca gcgagattga ggtggaaagt aacatctagg gccatttacc
     6541 ctgggtgctt agagccctca gagcagaggt tgcatgggga cagaactgtc aggaatgtcc
     6601 ttggaactca gaggtgctcc tggacaaagg ccagctcagt agccaggagt ccctggagtc
     6661 tgagatgctg tgtttgcagg ccaggtcttt gaggatgcca gggacctggg tggagagcag
     6721 agacccatcc actccagaac ctgaagtcat tgtggcacca gggaggaagt cctggtcaac
     6781 cacaggccag ccaagcagct gtggagacgc agggagtggg ggatcctggg agggcgggga
     6841 aagcaggcag ctaccagcag agggacctca tagagaccac attggaaacc tcgcatttcc
     6901 tctgcgtatg gccacagctg tttccagact tccagccagg agtcgtgttt ttgctaaaga
     6961 cagacactaa gatggaagcc ttagaagatg aacttcatga aagacagaac tccaggatcg
     7021 ggagaatttt cccctctagg aggggtggca tggttcagtg aggggatcaa agacctgggt
     7081 tcacatctgg ctgagtgacc tctggcatag tcctggcagg cccttggcaa atggggacag
     7141 tgacagtagg actgttgaaa ggcctgcagt gtgcctagag accacaaaac ccccagtggc
     7201 catgactcac tacaggggct agactccatt ctggggcact cgttctggtt ggtcagtgca
     7261 gtaatagtgg ggagaccaac agataaagcc ctcagctaac catcagggaa cccagagaca
     7321 ccagtcccat gaactacagg aacaagatgc aagctccggc tccctccacc ctcagtgccc
     7381 accactgata tggcctctga aggctgtgtc ctggctccct cccctgcccc gcccccctgc
     7441 catttacatc tttagtgtgt ggtgtgtatg tgtatgggcg tgtacctgga gatcagagga
     7501 caacttgtgg agatctgttc tctccttcta ctatgtaggt cccaggccat cgggcttaga
     7561 ggctggcacc ttcacccact gagccatctc actagcagcc ttccatgttg attcttagct
     7621 tcgtctaggg cctgtcaagt cccgccctta atcttgtgtt aacaatatcc atgtatttgt
     7681 ctaccgaggg gacagaagtg agggtgcttg tcactgccac ttttggttgg agactctcat
     7741 gactctctgt ctccagggaa ttccacggat caagccctga ggttttgcct cgtgtcctcg
     7801 tcctgtcttt tccagctgga gcagttggat tacccaatga gggagggaac aggcagacct
     7861 agtttggagc cgggtggggt ccgaacaggc cttggcagtg acaacttaag agatgccatg
     7921 ggatgtcttt tgtttttcag aacccaagag atgaagggtc tcctcacact ggcttggttc
     7981 ctggcttgca gtgagtactg gctctatgac ctctaaatag gaccctcccc cctcccccaa
     8041 ccttgggagt gaccgggaag tggaaaagaa ggcgatcatt attagccttt gaggtgagga
     8101 gatctgtgtc tcctgcttag ctctaccctc atggtgcagg cagagatatc ggcaaaggcc
     8161 ttggcctccc cgggtccttg cttttgcgtt ggtggacacg gtgctgtgtc atggttggaa
     8221 cactgtaggc catggagtga aggctgtgaa catgaagata gcttgcgaat gtaggaggca
     8281 gatatttccc tgatgtgtaa gaatagaata aggacaatca gtgggaacaa agtggaacac
     8341 aaaaccctta ggaaacatcc cagctccggg ctgggagcag ctcagtagtg gaggatgatg
     8401 cattaggttt aaggaaggaa gggggagatg gatggacaga tggacggaca gacagacaga
     8461 gacagactcc aatagatgat accagagtca tcttctgttc agcactgaat atcaccccac
     8521 ctgagaggtg tctgaaatgt ctttgggttg tcactggaga aggctgtgga tgtgggttta
     8581 agccgagaca aagtattcat tagctggctg gttgctctca gtgtagcccc gagccttact
     8641 cagggcgagc ttttaacaca aaacctgtat cccggggttg tcatgcttca gttaacaaga
     8701 acaattagcc agaagtagaa ctaccaaagc caaaaggcaa ggttagtaca gtgagagact
     8761 ttcccagaat tctggacttt gatggattac gtcattgttt tcattttggc tggcggtgct
     8821 gtctacacgc ctgctttaca gcttcaatgg tgcgtccatc atggagtcag ttgtgctaag
     8881 gtctgggggc ctgttacagg acatctagct ttaggcttca ctgacacccc ctagcctctc
     8941 atacaagccc catggtgagc taccctaccc tttgatgtct tgctggctgt ctggagagct
     9001 aggagaaact tgagatctga agcagagaaa aggcccctgg ctccttgcat caaaagtcct
     9061 aggcgagcta cttccttcta gatttccagt ttcccaatta aggcgcataa cgataccacg
     9121 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa
     9181 gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact
     9241 tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt
     9301 gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg
     9361 cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt
     9421 cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg
     9481 ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa
     9541 acttggcctc accaggcctc agccttggct cctcctggga actctactgc ccttgggatc
     9601 cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta
     9661 ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata
     9721 gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg
     9781 ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac
     9841 atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc
     9901 acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg
     9961 gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg
    10021 gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac
    10081 aaccaggtcc caggtcccga aaaccaaaga agaagaacgc agatcgcaga tcgcagatct
    10141 ccgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctggga
    10201 tctggactct agaggatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    10261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    10321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct
    10381 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg
    10441 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca
    10501 acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt
    10561 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
    10621 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
    10681 gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa
    10741 accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata
    10801 tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa
    10861 tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg
    10921 ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc
    10981 agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc
    11041 gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga
    11101 gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc
    11161 tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc
    11221 tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc
    11281 tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga
    11341 acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt
    11401 gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc
    11461 caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc
    11521 gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat
    11581 caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc
    11641 gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga
    11701 tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa
    11761 aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga
    11821 tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt
    11881 tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg
    11941 gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct
    12001 gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac
    12061 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
    12121 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
    12181 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
    12241 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
    12301 aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc
    12361 tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca
    12421 ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt
    12481 caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg
    12541 atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg
    12601 accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt
    12661 tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt
    12721 ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg
    12781 gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga
    12841 ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc
    12901 cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat
    12961 tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc
    13021 gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct
    13081 acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca
    13141 catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt
    13201 cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
    13261 aggggatccc ccgggagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
    13321 ccggccctat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    13381 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    13441 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    13501 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    13561 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    13621 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    13681 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    13741 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    13801 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    13861 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    13921 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    13981 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    14041 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    14101 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    14161 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    14221 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    14281 cttcgcccac cccccggatc taagctctag ataagtaatg atcataatca gccatatcac
    14341 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    14401 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    14461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    14521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgggggtacc gcgtcgagaa
    14581 gttcctattc cgaagttcct attctctaga aagtatagga acttcgtcga gataacttcg
    14641 tatagcatac attatacgaa gttatgtcga gatatctaga cccagctttc ttgtacaaag
    14701 tggttgatat ctctatagtc gcagtaggcg gtatgagtga gctagcttca agctatggcc
    14761 tactctgctt ctaaatttaa ttaaaaaaaa tggtttttta gatagcagct cacgtagacc
    14821 aggctagcct tgactttgcg tagccgagga tgaccacaaa cttcttaacc tcctgcttct
    14881 acctcctgag tgtaaggagg ctacacattc agtttacacg atgcctgtgg tcaaagccag
    14941 gttcagtcac gctaggcgag tcttctgtca gctgtgccac atccttcggc tcccaagtgc
    15001 aaacccttct gttccaggct gcatgctgtc atctgcccga ccctcctccc attaactggg
    15061 ataataagag agggatggga gggtggcggg gagcaagcag atctgcagca aggtgcctgg
    15121 tgtcacctgc aggcttgggc tgttttccag gtgtgcctgc agtcccaggg ggcttgctag
    15181 aactcaagtc catgattgag aaggtgacca ggaagaatgc ctttaagaac tatggcttct
    15241 acggctgcta ctgtggctgg ggcggtcgcg ggacacccaa ggatggcact gattggtgag
    15301 ctgacatcat caccacactt aatgttctgg gctccaccct catcacctcc cctgactcca
    15361 tgtctattct aaaagacgag aattacagct gaggctcagg accagagaga gagctcagtg
    15421 gttagagtga acaccactcc cgctaggcga cctaagttcg tttctgagat ggcgcaacgc
    15481 aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag ctcgccatca
    15541 gttcatgcac ttgcacacgt acgcacacac aattaaaact acaataaatt tctatttaac
    15601 tttttttgga gaaggggtct gactgtgtag ctttggctat cctggaactc actatgtctt
    15661 acactcaaag agattcatct gcctctgcct ccgagtcctg agactaaaac tgtgcgccac
    15721 tctgcctggc ttctaaaaga aatctttaaa ataagtaaag ccaaggcttt ctggatgtaa
    15781 gcagctttta aaggagcgac acatgactct caggagaccc tagatctcag tggcctaatg
    15841 tgtcacttgc tttagtgaaa tagaacaaat gatccttctg tgttggctta atgcaaccat
    15901 gactgtttac tttgctcatg tgcctgctgc ctgggaggcc atggctcagt gtccttttgc
    15961 aaatccatcc agtgatgaag ccagttatca actctcatga tctaatctct ggaaaagccc
    16021 tcagagacac atccaagaat gggattctat aatttcctag gccgctatta atccagtcaa
    16081 gttgatggtc aaggtcaaca ccgcattgtg cctaccacta tgtctagata ctgtgtctca
    16141 gctttcagag agcaagaggg gatctggact tttgctagag cagtgtagcc atcctagtag
    16201 actatgcgag atgggacatg ttgttccttg gaaggctcag tgtacatggt taacagtttc
    16261 atgccggcgg gagtcatgat ggagggcatc agatgacatt agggagctgg ggccacagtg
    16321 aagctcgact ctatggagaa gcaaggggag ggcttagaat gacctatgca gaaatggatt
    16381 gtagttgaac acatcgttat aaactcctat ttagcttagt ctgtgattat agaaatttat
    16441 agatatgggg ctggagtaat gacttggtca gcaaggtctt gctgtacagc atgaggctct
    16501 gaggttcgga tgctgttgag cgaggcggtg gagacaggcg catccctgga tctcgatgga
    16561 cagccacatt ggctgaatca gagagtctca ggttccagtg agagaccgta tctcagagag
    16621 taaggtagag agtggctgat ggagacacgt gacattgacc cctgaccttc acgggtgttc
    16681 agggccactg ctgacatcat gcctggcctc agcagctctc cttagtcata gagggacatt
    16741 ctacagcccc tttcctggat cgttaactct aaagccagaa tcacatggct gaggctgcca
    16801 agttcggctg cttccttgga gccagagcct gaccccctcg ttcaaatata ttttcaccag
    16861 ctttctcttt ttgtttgttt ccttcactgc ctaagcctgg aggtcctgga tctcctctgt
    16921 agaccaggct ggctttgaac tcagagattt gccagcctct gcctccccag tgcagattaa
    16981 agggattaaa agtgtgtgcc acagttactt ggccctaaac tgttcctgaa ttcctttcac
    17041 aagctgggtg ggactgtgcc gtgaggacac ctttccccat attccacccc acttcaggct
    17101 tttctttaat ctatatatct ccttgaaacc caggatttag taccattcca cttcctggtg
    17161 cctctttcat cctccaactg tacatttgat cagcttgccc ctcttcatta cagatctgca
    17221 taagatcagt gttctattgc tgtgaaggga caccatgacc acagcgactc ttgtaaagga
    17281 aaacattcaa ttgggggtgg cttacagttc agaggtttaa tctgttatcc tcacagctgg
    17341 aagcatggct gcatgcaggc agatatggtg ctggagaggt atctaagagt tctacatcca
    17401 gatctgcagg cagcaggaag gggatgtcac actagacctg gcttgagcat ctgagacttc
    17461 gaagttcacc ctcagtgaca cacttcctcc aggaccacat ctactccaac aagaacacac
    17521 ctcctaatag tgccagtccc catgagcctc aggggggggg gtagggcatt ttcattcaaa
    17581 ccaccacacc tccactgttt agctattagg aggcaaaacc aggtctgtga acttctgacc
    17641 agtggcagtt ctagtgggtg agaggagcta acttaccagg caccatcgct gctgggtcac
    17701 acccacttgc cacaggttct accaaaggga tgtggctgta gtttcatacc tgctaggggt
    17761 ttcagcctaa aaagcattca gactctccaa agaatagcta ttgatttggg aacagggctg
    17821 cattgcagta agattatgaa tgctgaaacc tatttgagga gggtgagcta aggggaagtt
    17881 taaattagca ggagagcaca acacctttac ataagctgct tagataccaa gctcattggt
    17941 tgtggaaacc agaggcaaga gctgccgtct gtttattggt ggagattctg ttgctaagca
    18001 agccttctat gtagagcatc ttgcctgagc tgtcgcagtc ttagagaaaa gtcaaacttt
    18061 gtcccaggta tctgtgctca caggcaaggc atgggaagct tggagtgcat acgggacgag
    18121 agagaacttc tcatcagggt gagaggttta aaaaaaacct tcagagagaa tttagcttta
    18181 acatccaagc acagtgtccc caaggtacca ttggctctaa ttttgtttag atttggctgg
    18241 aactctctca ctttggactc tcccacaggt gtgggggagt caggagggtg gctctggact
    18301 cagcatcccc atctctcacc tcgacaggtg ctgtcagatg cacgaccgtt gttatgggca
    18361 actggaggaa aaagactgtg ccattcggac ccagtcctat gactacagat acacaaatgg
    18421 cctagtcatc tgcggtaagg gtggcttctt attcagcctg ggagttctct gcccgagagg
    18481 tgggcctgcc tatatcttgt gaagcagagt gaggttgagg gacacagggc tgtgacaacc
    18541 tctttagact tccaggaaag ggaagtccca gttgagactc agtattcgcc agtactgtgg
    18601 gcttatagac ttcctctccc tggagagagt tggtttcatc ttctttactt cacaagtgta
    18661 atgacagctc aaaggttggc cagtgtgcaa caggatccct gcccaggccc ctaaatccaa
    18721 agccagttgc atccaggtgc atctctattg aatgcctccc acagaaactg gttccaccct
    18781 caagctaggc actctaccca aagggggtgt gtctatggca ccaggctggg gactccctga
    18841 atagaaattt cagtcatgat caagaagagc tgagcatcta gttctgcagc gggactcctc
    18901 cctcggatta cacatctcag ttcaggacct gaggctcttc cattagatta tgtatggccc
    18961 cgattcagag tcctcctcca cacactatga gatccctcag agcaggaccc agacggggag
    19021 tctatgcaga ctagaggcca tcctctgata cgaagttgaa caagtcagca ttgatgggtc
    19081 cagtcttcct atgctcattt tgtcctcggc atcccttttt gcagaacacg actccttctg
    19141 tccaatgagg ctctgtgcct gtgaccggaa gctggtctac tgcctgcgga gaaacctctg
    19201 gacttacaac cctctttacc agtattaccc caacttcctc tgctaatgtc cgctgtgcgc
    19261 tctgccctgg gggcacctcc cacggtggca gtctccctct gctgtattcc tgatgtgtcc
    19321 cgcctccccc acaaggtctt ggatctgcct tcctctgtgc accagtggac tggacagagc
    19381 cccccccccc ccccagggct acactcttcc ctccagagtc cgaggaaggg actttgatct
    19441 agagttggca gactctcagg atggctgagc ctgcacttgc taagttggct ctgggccctg
    19501 aagctccccc ggctcaggcc agccgtgttg acttttcttt caatttctgg aacccaactg
    19561 tcattagcac cctccagaga ccttttactg gaggagaagc caaattaact ctataaatct
    19621 gctatgtaga tattaaataa aacccattct caaggctaga aaaacaccac cccagcactt
    19681 cctctgacgg ggctggggtg ggattgccca cgcttttcca accccccccc ccccccgagg
    19741 catctgtatg ctctcgggga tggaggtcag gaaacaggtg ggagccttga cgccttgatg
    19801 cctttcatgg tttgtcttga gttcattttc ttcaacctta ggctccttca agccagacct
    19861 ggggtttatg tttcttctgg aggaagatga gacacagccc tatgccacct tgagctcctg
    19921 gctagagagg gcaacaatca gctcccagcc ctggcttctg gcagctgtat ggtcttttaa
    19981 acctttggat agtccgaatc tctctggctc tcctcaaaat ataaaacaag gctccttcca
    20041 ggagtatact ggtgcacacc ccaatcccag cacctggaag gtggaggcaa gagaatcggg
    20101 agttcaaggc cagcctctgc tccctagcag ggatagtagc ctagactatg tgagactgtg
    20161 tttcagaaag gaccctggta cagatacagg ggagctgggg ttctgagatg tcactcagtg
    20221 agaaaaatgg tttaaataag attcgggggg ggggggtaga gatatatcaa tggcggaatg
    20281 tctgtctatt tttcaccgag ttaggggctc tgaaaatctc aagtcatcta tcgctttata
    20341 aaagacagaa ctttctcata gcaacaagac aggaggaaga gatgtat
//