LOCUS       KO-first_condition_ready_39555_MGI:101899        22040 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Pla2g5 targeting project(s): 39555
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 39187
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8104..8153
                     /db_xref="ENSEMBL:ENSMUSE00001271923"
                     /rank="3"
                     /note="ENSMUSE00001271923"
     misc_feature    4275..9262
                     /note="5 arm"
     misc_feature    9290..9314
                     /note="B1 Gateway"
     misc_feature    9327..9374
                     /note="Frt"
     intron          9375..10249
                     /note="mouse En2 intron"
     misc_feature    10247..10249
                     /note="Splice Acceptor"
     exon            10250..10422
                     /note="mouse En2 exon"
     misc_feature    10434..11027
                     /note="ECMV IRES"
     gene            11028..14088
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14127..14370
                     /note="SV40 polyadenylation site"
     misc_feature    14374..14407
                     /note="loxP"
     promoter        14434..14953
                     /note="human beta actin promoter"
     gene            14979..15773
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15973..16214
                     /note="SV40 polyadenylation site"
     misc_feature    16231..16278
                     /note="Frt"
     misc_feature    16285..16318
                     /note="loxP"
     misc_feature    10264..10341
                     /note="FCHK std_gpr"
     misc_feature    16334..16354
                     /note="B2 Gateway"
     misc_feature    9263..16384
                     /note="Synthetic Cassette"
     exon            16804..16948
                     /db_xref="ENSEMBL:ENSMUSE00000181793"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000181793"
     misc_feature    16385..17118
                     /note="Critical Region"
     misc_feature    17142..17175
                     /note="Downstream LoxP"
     misc_feature    17119..17198
                     /note="synthetic loxP region"
     exon            20778..22040
                     /db_xref="ENSEMBL:ENSMUSE00000661792"
                     /rank="6"
                     /note="ENSMUSE00000661792 5' fragment"
     exon            19981..20087
                     /db_xref="ENSEMBL:ENSMUSE00001228294"
                     /rank="5"
                     /note="ENSMUSE00001228294"
     misc_feature    17199..22040
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggactacag gctcctgaga aatttggggt cggcaagtta gaaaggagag
     4321 aaagaagggc tgccctcgag ctgaggccga agaagacaga acaaaataga agtggttctg
     4381 tgggctggga aaccacaagg aaccaagggc tggatcattc cacaaaggcc cactgtggga
     4441 gcggaagtga ggctgtgcct gggaggggat ttcagggctc tgtctctgga tggggactag
     4501 tctcagaccc acgtcagctc aaagcagcca cttccagata tctcccctgt gagcgcccca
     4561 gctctgaggg ctgtatcaag aatctagaga aggagaaacc acagggggtc ttgaagtggg
     4621 cctccctggg tccccaggaa tcttggaggc ggcccacgca gcagtgtggg aggcgacagg
     4681 actggtttaa aaccaagcag acccacctgc cgcctcttct ccggaactca ggacagagac
     4741 tagcaatcag gtgagactct cattatggga ctgcccaatg ggggcggctg ggggccctgt
     4801 ccctctgtcg ggaagcagcc agcacagcgg gcagatatct caaagctgag tggaccattg
     4861 gactctttcc caggcaagag atgaaaatgg aggtccaagg cctacagggc tcccgcaggt
     4921 ggccttgaag gacccgttcc tcacgttgat cttggcagct ccatcacttc cacatcaccc
     4981 tccattctcc cgtcttgtcc atatcggctc tcctcacctt tcatcatcgt agcctggctc
     5041 caccctgaaa ttatctcgca gtgtccctgt gcccctgtgg tcatcggata aaaacagaca
     5101 agccataacc ttgtctcagg gctgccttgg ggctgcagag atgctctgga ctcctgcaca
     5161 gccgtttcca ggcccagcca ctgccgacac ctccctggaa tttgtcttat ctttgcaaat
     5221 ccttttataa ggactcctgc agtacccacg cagcaacagc ccagcccagg gaggtctggg
     5281 tgaggacagt ccacaggaga agagagttgg ctgtcacggg tgtgagtctg cagggaaaga
     5341 gagcaggatg ggctaactag cgagattcac ctcagtagct ctaggtttcc tgtcagaggc
     5401 cattgggcta acattgactg gcctggcctg aaagggtttt aagtatgtgg gtgctgggat
     5461 tttgaaggtt ttgactggtc tgactggtat gtaaaaatca tacctgcagg agagccaggt
     5521 cttagctgga ggaactggct agcctacagg gggcacattc tctttatcaa gacccttgag
     5581 atgtcaaagc gttatgaaat agaataccca ggcagggaat gtagctcagt tggcagagca
     5641 ctcgcctcgc gtgcagtagg ctctggggtc cacccagcac cgtacaaatt gggcatggtg
     5701 gtgcatgcct gtaatcccgg ctctttcttg aggggtggag gcaggaggat cacgaatttg
     5761 actacatagt tcaaggtcag tctgggctcc atgacaccct gtctaaatga aaaggaagga
     5821 agagaaaaaa gaaagaaagg aaggaaggaa agatgagagg aaaagaacaa ggaaggaaaa
     5881 aaaggcaaaa agaaaacagg aaaagaggag tggaggtcgc catagagaga tggaggagaa
     5941 tgctcaggac aggattctct agcttgggct aaacctaaac ctttgggcaa ctgtttcacc
     6001 tacatggggc ttgttcatcc tctcctctcc tctcctctcc tctcctctcc tctcctctcc
     6061 tctcctctcc tctcctctcc tctcctctcc tctcctctcc tctcctctcc tctcctctcc
     6121 tttcctctcc ccttccctcc cctaacccaa tctcccctcc ctccccgcct catatatata
     6181 tatacacata tgcatatata atgtatatat atatatatac atatatatat atatatatgt
     6241 acagaagttg aatctggaga gtctggagtc aggctgacag ccagccagtc ccagtgaacc
     6301 ccctgtctgt gccccacacc actggggtta gaggcagagg caaataccca ctcagcttgc
     6361 ttatgtggcc actagaaggc aaactcagat tctcaggctt gcacagtgag ggtttttaac
     6421 tcaccaaagc tacctcccca gcccctggcc tgagcctgac agttcctcag tctgtaaaat
     6481 caataactct tatccattca tccattcatc tgacacagat ccccattgaa ggggacatac
     6541 agtggcttgc cagtgtgtac ccatcaaggc catgagtggt ttgagagtga cgtggatggt
     6601 gtctggcatc ctgaagatac ccagtgtgta gtctctggtg tctctgcttc aaaaaagcaa
     6661 acagcgagat tgaggtggaa agtaacatct agggccattt accctgggtg cttagagccc
     6721 tcagagcaga ggttgcatgg ggacagaact gtcaggaatg tccttggaac tcagaggtgc
     6781 tcctggacaa aggccagctc agtagccagg agtccctgga gtctgagatg ctgtgtttgc
     6841 aggccaggtc tttgaggatg ccagggacct gggtggagag cagagaccca tccactccag
     6901 aacctgaagt cattgtggca ccagggagga agtcctggtc aaccacaggc cagccaagca
     6961 gctgtggaga cgcagggagt gggggatcct gggagggcgg ggaaagcagg cagctaccag
     7021 cagagggacc tcatagagac cacattggaa acctcgcatt tcctctgcgt atggccacag
     7081 ctgtttccag acttccagcc aggagtcgtg tttttgctaa agacagacac taagatggaa
     7141 gccttagaag atgaacttca tgaaagacag aactccagga tcgggagaat tttcccctct
     7201 aggaggggtg gcatggttca gtgaggggat caaagacctg ggttcacatc tggctgagtg
     7261 acctctggca tagtcctggc aggcccttgg caaatgggga cagtgacagt aggactgttg
     7321 aaaggcctgc agtgtgccta gagaccacaa aacccccagt ggccatgact cactacaggg
     7381 gctagactcc attctggggc actcgttctg gttggtcagt gcagtaatag tggggagacc
     7441 aacagataaa gccctcagct aaccatcagg gaacccagag acaccagtcc catgaactac
     7501 aggaacaaga tgcaagctcc ggctccctcc accctcagtg cccaccactg atatggcctc
     7561 tgaaggctgt gtcctggctc cctcccctgc cccgcccccc tgccatttac atctttagtg
     7621 tgtggtgtgt atgtgtatgg gcgtgtacct ggagatcaga ggacaacttg tggagatctg
     7681 ttctctcctt ctactatgta ggtcccaggc catcgggctt agaggctggc accttcaccc
     7741 actgagccat ctcactagca gccttccatg ttgattctta gcttcgtcta gggcctgtca
     7801 agtcccgccc ttaatcttgt gttaacaata tccatgtatt tgtctaccga ggggacagaa
     7861 gtgagggtgc ttgtcactgc cacttttggt tggagactct catgactctc tgtctccagg
     7921 gaattccacg gatcaagccc tgaggttttg cctcgtgtcc tcgtcctgtc ttttccagct
     7981 ggagcagttg gattacccaa tgagggaggg aacaggcaga cctagtttgg agccgggtgg
     8041 ggtccgaaca ggccttggca gtgacaactt aagagatgcc atgggatgtc ttttgttttt
     8101 cagaacccaa gagatgaagg gtctcctcac actggcttgg ttcctggctt gcagtgagta
     8161 ctggctctat gacctctaaa taggaccctc ccccctcccc caaccttggg agtgaccggg
     8221 aagtggaaaa gaaggcgatc attattagcc tttgaggtga ggagatctgt gtctcctgct
     8281 tagctctacc ctcatggtgc aggcagagat atcggcaaag gccttggcct ccccgggtcc
     8341 ttgcttttgc gttggtggac acggtgctgt gtcatggttg gaacactgta ggccatggag
     8401 tgaaggctgt gaacatgaag atagcttgcg aatgtaggag gcagatattt ccctgatgtg
     8461 taagaataga ataaggacaa tcagtgggaa caaagtggaa cacaaaaccc ttaggaaaca
     8521 tcccagctcc gggctgggag cagctcagta gtggaggatg atgcattagg tttaaggaag
     8581 gaagggggag atggatggac agatggacgg acagacagac agagacagac tccaatagat
     8641 gataccagag tcatcttctg ttcagcactg aatatcaccc cacctgagag gtgtctgaaa
     8701 tgtctttggg ttgtcactgg agaaggctgt ggatgtgggt ttaagccgag acaaagtatt
     8761 cattagctgg ctggttgctc tcagtgtagc cccgagcctt actcagggcg agcttttaac
     8821 acaaaacctg tatcccgggg ttgtcatgct tcagttaaca agaacaatta gccagaagta
     8881 gaactaccaa agccaaaagg caaggttagt acagtgagag actttcccag aattctggac
     8941 tttgatggat tacgtcattg ttttcatttt ggctggcggt gctgtctaca cgcctgcttt
     9001 acagcttcaa tggtgcgtcc atcatggagt cagttgtgct aaggtctggg ggcctgttac
     9061 aggacatcta gctttaggct tcactgacac cccctagcct ctcatacaag ccccatggtg
     9121 agctacccta ccctttgatg tcttgctggc tgtctggaga gctaggagaa acttgagatc
     9181 tgaagcagag aaaaggcccc tggctccttg catcaaaagt cctaggcgag ctacttcctt
     9241 ctagatttcc agtttcccaa ttaaggcgca taacgatacc acgatatcaa caagtttgta
     9301 caaaaaagca ggctggcgcc ggaaccgaag ttcctattcc gaagttccta ttctctagaa
     9361 agtataggaa cttcgaaccc tttcccacac caccctccac acttgcccca aacactgcca
     9421 actatgtagg aggaaggggt tgggactaac agaagaaccc gttgtgggga agctgttggg
     9481 agggtcactt tatgttcttg cccaaggtca gttgggtggc ctgcttctga tgaggtggtc
     9541 ccaaggtctg gggtagaagg tgagagggac aggccaccaa ggtcagcccc ccccccctat
     9601 cccataggag ccaggtccct ctcctggaca ggaagactga aggggagatg ccagagactc
     9661 agtgaagcct ggggtaccct attggagtcc ttcaaggaaa caaacttggc ctcaccaggc
     9721 ctcagccttg gctcctcctg ggaactctac tgcccttggg atccccttgt agttgtgggt
     9781 tacataggaa gggggacggg attccccttg actggctagc ctactctttt cttcagtctt
     9841 ctccatctcc tctcacctgt ctctcgaccc tttccctagg atagacttgg aaaaagataa
     9901 ggggagaaaa caaatgcaaa cgaggccaga aagattttgg ctgggcattc cttccgctag
     9961 cttttattgg gatcccctag tttgtgatag gccttttagc tacatctgcc aatccatctc
    10021 attttcacac acacacacac cactttcctt ctggtcagtg ggcacatgtc cagcctcaag
    10081 tttatatcac cacccccaat gcccaacact tgtatggcct tgggcgggtc atcccccccc
    10141 ccacccccag tatctgcaac ctcaagctag cttgggtgcg ttggttgtgg ataagtagct
    10201 agactccagc aaccagtaac ctctgccctt tctcctccat gacaaccagg tcccaggtcc
    10261 cgaaaaccaa agaagaagaa ccctaacaaa gaggacaagc ggcctcgcac agccttcact
    10321 gctgagcagc tccagaggct caaggctgag tttcagacca acaggtacct gacagagcag
    10381 cggcgccaga gtctggcaca ggagctcggt acccggaaga tctggactct agagaattcc
    10441 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
    10501 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
    10561 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
    10621 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
    10681 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
    10741 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
    10801 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
    10861 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
    10921 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
    10981 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaaccatg gaagatcccg
    11041 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    11101 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    11161 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg
    11221 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa
    11281 actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg
    11341 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg
    11401 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg
    11461 cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt
    11521 ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc
    11581 tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt
    11641 tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca
    11701 ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg
    11761 agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca
    11821 gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg
    11881 tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct
    11941 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg
    12001 atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt
    12061 tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg
    12121 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct
    12181 gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg
    12241 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg
    12301 atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta
    12361 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg
    12421 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg
    12481 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag
    12541 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag
    12601 agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt
    12661 tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg
    12721 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg
    12781 gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg
    12841 ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt
    12901 tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata
    12961 acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc
    13021 ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg
    13081 agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt
    13141 cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga
    13201 cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca
    13261 tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt
    13321 ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc
    13381 tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac
    13441 gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata
    13501 cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct
    13561 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg
    13621 atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg
    13681 cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc
    13741 gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt
    13801 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
    13861 accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg
    13921 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
    13981 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc
    14041 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca
    14101 ggggggatct aagctctaga taagtaatga tcataatcag ccatatcaca tctgtagagg
    14161 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    14221 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    14281 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    14341 tcatcaatgt atcttatcat gtctggatcc ggaataactt cgtatagcat acattatacg
    14401 aagttatgtt taaacggcgc gccccggaat tcgccttctg caggagcgta cagaacccag
    14461 ggccctggca cccgtgcaga ccctggccca ccccacctgg gcgctcagtg cccaagagat
    14521 gtccacacct aggatgtccc gcggtgggtg gggggcccga gagacgggca ggccgggggc
    14581 aggcctggcc atgcggggcc gaaccgggca ctgcccagcg tgggcgcggg ggccacggcg
    14641 cgcgccccca gcccccgggc ccagcacccc aaggcggcca acgccaaaac tctccctcct
    14701 cctcttcctc aatctcgctc tcgctctttt tttttttcgc aaaaggaggg gagagggggt
    14761 aaaaaaatgc tgcactgtgc ggcgaagccg gtgagtgagc ggcgcggggc caatcagcgt
    14821 gcgccgttcc gaaagttgcc ttttatggct cgagcggccg cggcggcgcc ctataaaacc
    14881 cagcggcgcg acgcgccacc accgccgaga ccgcgtccgc cccgcgagca cagagcctcg
    14941 cctttgccga tcctctagag tcgagatccg ccgccaccat gattgaacaa gatggattgc
    15001 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
    15061 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
    15121 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
    15181 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
    15241 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
    15301 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
    15361 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
    15421 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
    15481 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
    15541 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
    15601 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
    15661 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
    15721 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
    15781 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
    15841 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    15901 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccccgg atctaagctc
    15961 tagataagta atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa
    16021 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    16081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    16141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    16201 tcatgtctgg atccgggggt accgcgtcga gaagttccta ttccgaagtt cctattctct
    16261 agaaagtata ggaacttcgt cgagataact tcgtatagca tacattatac gaagttatgt
    16321 cgagatatct agacccagct ttcttgtaca aagtggttga tatctctata gtcgcagtag
    16381 gcggtatgag tgagctagct tcaagctatg gcctactctg cttctaaatt taattaaaaa
    16441 aaatggtttt ttagatagca gctcacgtag accaggctag ccttgacttt gcgtagccga
    16501 ggatgaccac aaacttctta acctcctgct tctacctcct gagtgtaagg aggctacaca
    16561 ttcagtttac acgatgcctg tggtcaaagc caggttcagt cacgctaggc gagtcttctg
    16621 tcagctgtgc cacatccttc ggctcccaag tgcaaaccct tctgttccag gctgcatgct
    16681 gtcatctgcc cgaccctcct cccattaact gggataataa gagagggatg ggagggtggc
    16741 ggggagcaag cagatctgca gcaaggtgcc tggtgtcacc tgcaggcttg ggctgttttc
    16801 caggtgtgcc tgcagtccca gggggcttgc tagaactcaa gtccatgatt gagaaggtga
    16861 ccaggaagaa tgcctttaag aactatggct tctacggctg ctactgtggc tggggcggtc
    16921 gcgggacacc caaggatggc actgattggt gagctgacat catcaccaca cttaatgttc
    16981 tgggctccac cctcatcacc tcccctgact ccatgtctat tctaaaagac gagaattaca
    17041 gctgaggctc aggaccagag agagagctca gtggttagag tgaacaccac tcccgctagg
    17101 cgacctaagt tcgtttctga gatggcgcaa cgcaattaat gataacttcg tatagcatac
    17161 attatacgaa gttatggtct gagctcgcca tcagttcatg cacttgcaca cgtacgcaca
    17221 cacaattaaa actacaataa atttctattt aacttttttt ggagaagggg tctgactgtg
    17281 tagctttggc tatcctggaa ctcactatgt cttacactca aagagattca tctgcctctg
    17341 cctccgagtc ctgagactaa aactgtgcgc cactctgcct ggcttctaaa agaaatcttt
    17401 aaaataagta aagccaaggc tttctggatg taagcagctt ttaaaggagc gacacatgac
    17461 tctcaggaga ccctagatct cagtggccta atgtgtcact tgctttagtg aaatagaaca
    17521 aatgatcctt ctgtgttggc ttaatgcaac catgactgtt tactttgctc atgtgcctgc
    17581 tgcctgggag gccatggctc agtgtccttt tgcaaatcca tccagtgatg aagccagtta
    17641 tcaactctca tgatctaatc tctggaaaag ccctcagaga cacatccaag aatgggattc
    17701 tataatttcc taggccgcta ttaatccagt caagttgatg gtcaaggtca acaccgcatt
    17761 gtgcctacca ctatgtctag atactgtgtc tcagctttca gagagcaaga ggggatctgg
    17821 acttttgcta gagcagtgta gccatcctag tagactatgc gagatgggac atgttgttcc
    17881 ttggaaggct cagtgtacat ggttaacagt ttcatgccgg cgggagtcat gatggagggc
    17941 atcagatgac attagggagc tggggccaca gtgaagctcg actctatgga gaagcaaggg
    18001 gagggcttag aatgacctat gcagaaatgg attgtagttg aacacatcgt tataaactcc
    18061 tatttagctt agtctgtgat tatagaaatt tatagatatg gggctggagt aatgacttgg
    18121 tcagcaaggt cttgctgtac agcatgaggc tctgaggttc ggatgctgtt gagcgaggcg
    18181 gtggagacag gcgcatccct ggatctcgat ggacagccac attggctgaa tcagagagtc
    18241 tcaggttcca gtgagagacc gtatctcaga gagtaaggta gagagtggct gatggagaca
    18301 cgtgacattg acccctgacc ttcacgggtg ttcagggcca ctgctgacat catgcctggc
    18361 ctcagcagct ctccttagtc atagagggac attctacagc ccctttcctg gatcgttaac
    18421 tctaaagcca gaatcacatg gctgaggctg ccaagttcgg ctgcttcctt ggagccagag
    18481 cctgaccccc tcgttcaaat atattttcac cagctttctc tttttgtttg tttccttcac
    18541 tgcctaagcc tggaggtcct ggatctcctc tgtagaccag gctggctttg aactcagaga
    18601 tttgccagcc tctgcctccc cagtgcagat taaagggatt aaaagtgtgt gccacagtta
    18661 cttggcccta aactgttcct gaattccttt cacaagctgg gtgggactgt gccgtgagga
    18721 cacctttccc catattccac cccacttcag gcttttcttt aatctatata tctccttgaa
    18781 acccaggatt tagtaccatt ccacttcctg gtgcctcttt catcctccaa ctgtacattt
    18841 gatcagcttg cccctcttca ttacagatct gcataagatc agtgttctat tgctgtgaag
    18901 ggacaccatg accacagcga ctcttgtaaa ggaaaacatt caattggggg tggcttacag
    18961 ttcagaggtt taatctgtta tcctcacagc tggaagcatg gctgcatgca ggcagatatg
    19021 gtgctggaga ggtatctaag agttctacat ccagatctgc aggcagcagg aaggggatgt
    19081 cacactagac ctggcttgag catctgagac ttcgaagttc accctcagtg acacacttcc
    19141 tccaggacca catctactcc aacaagaaca cacctcctaa tagtgccagt ccccatgagc
    19201 ctcagggggg ggggtagggc attttcattc aaaccaccac acctccactg tttagctatt
    19261 aggaggcaaa accaggtctg tgaacttctg accagtggca gttctagtgg gtgagaggag
    19321 ctaacttacc aggcaccatc gctgctgggt cacacccact tgccacaggt tctaccaaag
    19381 ggatgtggct gtagtttcat acctgctagg ggtttcagcc taaaaagcat tcagactctc
    19441 caaagaatag ctattgattt gggaacaggg ctgcattgca gtaagattat gaatgctgaa
    19501 acctatttga ggagggtgag ctaaggggaa gtttaaatta gcaggagagc acaacacctt
    19561 tacataagct gcttagatac caagctcatt ggttgtggaa accagaggca agagctgccg
    19621 tctgtttatt ggtggagatt ctgttgctaa gcaagccttc tatgtagagc atcttgcctg
    19681 agctgtcgca gtcttagaga aaagtcaaac tttgtcccag gtatctgtgc tcacaggcaa
    19741 ggcatgggaa gcttggagtg catacgggac gagagagaac ttctcatcag ggtgagaggt
    19801 ttaaaaaaaa ccttcagaga gaatttagct ttaacatcca agcacagtgt ccccaaggta
    19861 ccattggctc taattttgtt tagatttggc tggaactctc tcactttgga ctctcccaca
    19921 ggtgtggggg agtcaggagg gtggctctgg actcagcatc cccatctctc acctcgacag
    19981 gtgctgtcag atgcacgacc gttgttatgg gcaactggag gaaaaagact gtgccattcg
    20041 gacccagtcc tatgactaca gatacacaaa tggcctagtc atctgcggta agggtggctt
    20101 cttattcagc ctgggagttc tctgcccgag aggtgggcct gcctatatct tgtgaagcag
    20161 agtgaggttg agggacacag ggctgtgaca acctctttag acttccagga aagggaagtc
    20221 ccagttgaga ctcagtattc gccagtactg tgggcttata gacttcctct ccctggagag
    20281 agttggtttc atcttcttta cttcacaagt gtaatgacag ctcaaaggtt ggccagtgtg
    20341 caacaggatc cctgcccagg cccctaaatc caaagccagt tgcatccagg tgcatctcta
    20401 ttgaatgcct cccacagaaa ctggttccac cctcaagcta ggcactctac ccaaaggggg
    20461 tgtgtctatg gcaccaggct ggggactccc tgaatagaaa tttcagtcat gatcaagaag
    20521 agctgagcat ctagttctgc agcgggactc ctccctcgga ttacacatct cagttcagga
    20581 cctgaggctc ttccattaga ttatgtatgg ccccgattca gagtcctcct ccacacacta
    20641 tgagatccct cagagcagga cccagacggg gagtctatgc agactagagg ccatcctctg
    20701 atacgaagtt gaacaagtca gcattgatgg gtccagtctt cctatgctca ttttgtcctc
    20761 ggcatccctt tttgcagaac acgactcctt ctgtccaatg aggctctgtg cctgtgaccg
    20821 gaagctggtc tactgcctgc ggagaaacct ctggacttac aaccctcttt accagtatta
    20881 ccccaacttc ctctgctaat gtccgctgtg cgctctgccc tgggggcacc tcccacggtg
    20941 gcagtctccc tctgctgtat tcctgatgtg tcccgcctcc cccacaaggt cttggatctg
    21001 ccttcctctg tgcaccagtg gactggacag agcccccccc cccccccagg gctacactct
    21061 tccctccaga gtccgaggaa gggactttga tctagagttg gcagactctc aggatggctg
    21121 agcctgcact tgctaagttg gctctgggcc ctgaagctcc cccggctcag gccagccgtg
    21181 ttgacttttc tttcaatttc tggaacccaa ctgtcattag caccctccag agacctttta
    21241 ctggaggaga agccaaatta actctataaa tctgctatgt agatattaaa taaaacccat
    21301 tctcaaggct agaaaaacac caccccagca cttcctctga cggggctggg gtgggattgc
    21361 ccacgctttt ccaacccccc cccccccccg aggcatctgt atgctctcgg ggatggaggt
    21421 caggaaacag gtgggagcct tgacgccttg atgcctttca tggtttgtct tgagttcatt
    21481 ttcttcaacc ttaggctcct tcaagccaga cctggggttt atgtttcttc tggaggaaga
    21541 tgagacacag ccctatgcca ccttgagctc ctggctagag agggcaacaa tcagctccca
    21601 gccctggctt ctggcagctg tatggtcttt taaacctttg gatagtccga atctctctgg
    21661 ctctcctcaa aatataaaac aaggctcctt ccaggagtat actggtgcac accccaatcc
    21721 cagcacctgg aaggtggagg caagagaatc gggagttcaa ggccagcctc tgctccctag
    21781 cagggatagt agcctagact atgtgagact gtgtttcaga aaggaccctg gtacagatac
    21841 aggggagctg gggttctgag atgtcactca gtgagaaaaa tggtttaaat aagattcggg
    21901 gggggggggt agagatatat caatggcgga atgtctgtct atttttcacc gagttagggg
    21961 ctctgaaaat ctcaagtcat ctatcgcttt ataaaagaca gaactttctc atagcaacaa
    22021 gacaggagga agagatgtat
//