LOCUS       KO-first_condition_ready_47370_MGI:101884        22061 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Ppard targeting project(s): 47370
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_P
COMMENT     design_id: 199202
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8758..8912
                     /db_xref="ENSEMBL:ENSMUSE00001303718"
                     /rank="4"
                     /note="ENSMUSE00001303718"
     misc_feature    4275..10092
                     /note="5 arm"
     misc_feature    10120..10144
                     /note="B1 Gateway"
     misc_feature    10157..10204
                     /note="Frt"
     intron          10205..11079
                     /note="mouse En2 intron"
     misc_feature    11077..11079
                     /note="Splice Acceptor"
     exon            11080..11081
                     /note="mouse En2 exon"
     misc_feature    11293..11854
                     /note="ECMV IRES"
     gene            11865..14911
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14946..15201
                     /note="SV40 polyadenylation site"
     misc_feature    15205..15238
                     /note="loxP"
     promoter        15270..15777
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            15864..16655
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16853..17096
                     /note="SV40 polyadenylation site"
     misc_feature    17113..17160
                     /note="Frt"
     misc_feature    17167..17200
                     /note="loxP"
     misc_feature    11094..11171
                     /note="FCHK std_gpr"
     misc_feature    17216..17236
                     /note="B2 Gateway"
     misc_feature    10093..17266
                     /note="Synthetic Cassette"
     exon            17640..17778
                     /db_xref="ENSEMBL:ENSMUSE00001276960"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00001276960"
     misc_feature    17267..17945
                     /note="Critical Region"
     misc_feature    17969..18002
                     /note="Downstream LoxP"
     misc_feature    17946..18025
                     /note="synthetic loxP region"
     exon            18869..19071
                     /db_xref="ENSEMBL:ENSMUSE00001300552"
                     /rank="6"
                     /note="ENSMUSE00001300552"
     exon            19176..19626
                     /db_xref="ENSEMBL:ENSMUSE00001312231"
                     /rank="7"
                     /note="ENSMUSE00001312231"
     exon            20194..22061
                     /db_xref="ENSEMBL:ENSMUSE00000242569"
                     /rank="8"
                     /note="ENSMUSE00000242569"
     misc_feature    18026..22061
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagacgtg aggataagtc aatagtgtgt gtttgctgag cttgtagtct
     4321 gatggcaaga ctgattgccg tgtggggtgc cagggccagg attactcaga gttcaagtgt
     4381 ctaaggaccg acctccgcca tcagtgctga gctgctctag agacaggaag attgtgcctg
     4441 tgggcagcag gagagtgcgc accgccaccc cgatcagggg ttcccagata gacagaccca
     4501 ataaagggag gggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt agagctataa
     4561 tggaggtggg gtcccctctt ccaggaggaa agaggaccca gccattctct cgaagccttc
     4621 gcctatagac agagagccac atgcattctg aactgttagc actctgtact ccagtctcct
     4681 gagttagatg acagtcacgt ctttatcttt ctctcctttt tgttttgttt tgttttttgt
     4741 ttgttttgtt ttgttttttg agatagtctc tctatatatt cctggctgtc ctggaactca
     4801 ctctgtagac caggctggtc tcaaactcaa agatccacct gcctctgcct cctaagtcct
     4861 gggattaaat gaatgcagac tagcctgtga acggaaacct ggctatcaca gcgcagccag
     4921 atcaggaaag ccgtcctcac cactatcttt aaccccatca accacagttc tcccgcctgt
     4981 tatagagggg cacagtgcct gacttgaagg gttatttcga agctttgcat gaaggaaggg
     5041 atgggcagta cccagtagag ttctggaaca caggcagtgc cagtgcaccc tctgctgcag
     5101 gtcccagtcc taagcacgac cagcaagtga ccttgcacaa aatgaaacga gccaggcatg
     5161 gtggcatttg ggaggcagag gcaggcgcag caccgtgagt tcacaactaa tccaggctac
     5221 ttaattagac catctcagac ccaaacaatc caaaaagacc cttggctagt aggtggggcc
     5281 tggcaaaagc cacctcttct cctgttcctc ccggtaaaca aagacccttg ggaggtgtct
     5341 caggcccctg gcagggattc tgggaccagc tgcaggcgta tcaggtgaac aaaggtatct
     5401 cctaagagca gcaggcagga gtgagaggag tgttaggtcc attgatgtgt caggatacct
     5461 catacagaca gccttaaaca gaaaggcttt attcaggaag acaatgctga aggttccagc
     5521 ccagccagcc aaccctacta ctttggggtc tatggtgagt catgcattgt aaagaagaga
     5581 cagggttcaa cgtgcctctc taaggctcac ctccagtgac cacggtagcg ggaggtcctg
     5641 cctcccgtta gcaccacaag ctttgaacat ccgggccttt agtcaacatt cagaatctat
     5701 gctatgacca ggcgtgtgac tatacgccta tagtcccagt tcccaggagg ctgagttaca
     5761 ggccagcctg gactccagaa atgagactgt gagtcaaggg aagaaaagag gctgaggatg
     5821 gggctcagtt ggtagagtgt taacctgctg tgctgcaggt gtggccccag cagtggggag
     5881 gctaaggagg cagaaacaga gctttaaggc cattacggaa atgactgcac acatacatga
     5941 ggacctgcac tcagatactt agcactcaca gaaagagcta ggcctgggcc agtgaggtgg
     6001 ctcagagggc gaaggcgctt gctgccaagc ttggctacca gggtttgatc ctgggactca
     6061 cacagtggaa gaagaaaaca agcttctcaa agttgtcccc cggcccctac atgtgcacac
     6121 gcaaatacat aaacttaaat gcagttttaa acattagaat caggggccga gagacggtcc
     6181 cacatttaac atctcttgcc actcctctag gggaaccaga ttcggttcct agcacccacg
     6241 tggtggctca caactgtcta taactccagt ttaggtaatc tggtaccctc ttctggcctc
     6301 catgggcact gcatgcacaa atgttcaggg agggagggag ggagggaggg agggagagag
     6361 agagagagag agagagagag agagagagag agagagagag agaacccaga cacatgtata
     6421 taaagaaaaa aagaaagaaa gaaagaaagt aaaaactttg gaattagaac taaatccaga
     6481 gtgttcgtat gacctgtgac cccagtgctg gggttaggag acaggagacc cctaggctca
     6541 ctgctcagtc agccaggcca ggcagagagc tcctggtttg gtgggaaacc atgactcaaa
     6601 aaatacagtg gaagtcatgc gtggtgtcca tcacctttaa tcccagcact caagaggcag
     6661 aggcaggagg atctctgagt ttgaggccag tgtggtctat aaatctagtt ccaggacaac
     6721 caggtctata cagagaaact gtgtctcaaa aagaaaataa aagaaaaaaa gacaacagtg
     6781 aggagtgata cacacacaca cagacacaca cacacacaca cacacacatg catgcatgtt
     6841 caaggctgac ctcagtcaca tagtaaattc aaaactagcc tgggtcacaa gagctattgt
     6901 ctcaaaaaaa aaaaaaaaaa gaggaaagag agaattcagg aagggaaaat agtgcccaca
     6961 gagcagaccc aaccttttgc accctcgccc tgaagagctt ggggcagtgt tctgtgtgaa
     7021 gaagggtctt atagaatccc aacgtccact tgtgcagtca ccgatctgcc acactctgat
     7081 cagggtgtaa cagagaaggc acaggaccat cacctccagg agctgtgcct gcagtggggt
     7141 cattctcctg ggtgtgatat tgtggtggta tgaggacaaa tcctcaagcc agtggccagc
     7201 caggaagaag cagaaacatt gtttttggaa aagccaccca gacactattt ctatccactc
     7261 agcacacata ctcagatgac gccatacccg tccccacagg tcctgcttct ctggccttgt
     7321 gtgtcaagtt acacatggct tggagccctc actcaagctg aggcattcca ccactcactg
     7381 gtgagctggg cctgtttctc tgtggtagta attcccctgg cctacctcgg gtagccagta
     7441 ggacctggtt atcttggcta atcacagggt tctggaggtg tacagacacc ccaccccatc
     7501 cccgagccag agagcactcc aggacagcca tggatgtggg gagacaagag gctgtaccgg
     7561 gtttctcctg gccctgcctg cacaggaggg gcttgccgtg gagagcacag acccagagag
     7621 ctggggagct tgccatgagc ttggtatcca caacttctca gccttgggcg tcagtgtgcc
     7681 cacatgagaa cgcacagacc ccatccccat cccttggtga aactaccagg aagtccaaag
     7741 ctgctttacc ctccaggagg gcctgccaga gaaaatgagg ggatcagctc tcctcagctg
     7801 gggccggagg gcatttatac ctttggaatg acagtcgtcc tgaactaggg ttccaggcca
     7861 cttatgacct tatgtaacac acgcaaactt tagcgtctgg gatggctata gcccccatgg
     7921 tcccttgctg cctgagttcc cctgctgtgg ccagtgtaca ggctgggagt gctggtctgc
     7981 gctgatcacg tggctgtggt ctctctcctg cttgagtgtc tctcactggt ctggggagtt
     8041 ggtgcctgtc tctgaggaag tgagtctgca gccccaggag ccagcccaga ctgtaagtaa
     8101 ggccagcaaa cggaagtgga tgggtagtac tgtgaagcca gtctcatcgc ttgtctcctc
     8161 cttaaatctc aaagtagact cagaagaggc tcgctggcgt ctagacagtt gtgtgacagc
     8221 aggtccatcc ctgtgaccgt gcagttacag cctcccactg tccttgccag ccttgaaacc
     8281 tcagcccaaa cctctgtgta cgcagtagaa cagggcaagc tggtgctgca cctgagctca
     8341 ctccagcagg acttctggga gagtcctgac agcaaccctg gggtccacag agacttgaac
     8401 tcctgggatg ggagccagct gggcggagct ccgcaccccc acagttccca acagacacac
     8461 tccaccccac catcatccgg gacccactga agatgctatt tacccaaagc ccaccaggcc
     8521 ccctcctctc ccagtcaggg aggctgtgag cggcagctgc tcagctgcct gcctagcggg
     8581 gcttcccccg accctgaact ggagaggagg ctgggccctt gaagcttttc cccagtcaca
     8641 cagcccatgg ctgctgagct cttcagctga gctctaggct gcagctcttc cttcccccac
     8701 ctggcttgta ggcctggtag ctgattgttt aactgcccct ccgtcctgtc ttcacagacc
     8761 tctcccagaa ttcctcccct tcctccctgc tggaccagct gcagatgggc tgtgatgggg
     8821 cctcaggcgg cagcctcaac atggaatgtc gggtgtgcgg ggacaaggcc tcgggcttcc
     8881 actacggggt ccacgcgtgc gaggggtgca aggtacagat ggactgaggg gcgtggggat
     8941 ttgcctgctt caggtaggac cccgaaaata gctccagcaa agctctttgg ttccttagag
     9001 gcctgagctc tgggtcatcc aagggaagaa aagacaggtg tccaaatgga cacctgccta
     9061 ctgacccagc tatccactca gacgctgtgg atggccagct ctggggcctg ggctccagca
     9121 ggcaggcagg gaaagagcaa gcttgggctg agtgtccatt gaagtcagtt tccagggcct
     9181 tgggtcttaa gaggatcttt ggcaagagag tgatgcttct aagttgttct aacgacacac
     9241 catcagaatt tcacaaagcg ccatctgaaa ggatggcgag aggcggctca gtgctgaaga
     9301 gcgcttgctg ctcttctgag gatccgagtt tgatttccag caaccatgtc tggtggttca
     9361 atctcctatt accccagctc cagtcctctt ctggcctgaa gtcacccaca ctcaacatgc
     9421 gtgcacgtgc ccacacacag gtacacatgc gcccacacgc aggcacacat gcattaaagt
     9481 atatctttca aattgccata tggggttgga aatgtgcttg cctgcctggg agaagccctt
     9541 ggtttgagcg acatcaccac aaaaactggg tgtggtgcta tacacggtaa tcttagggat
     9601 ttggagctaa aggcaagaag atcagctcaa agttgggctg gaaagatggc tcagcggata
     9661 gagctgctgc cttaagcctg gtgacccgag ttccgtttcc aagaaccaac tggaaagagg
     9721 gaaccaattc ttgtctgctg gccttcacat gcatagcccc ccacaccccc caaataaata
     9781 aataaataaa taaatgttaa ataaaccaag ttcttatgaa aacagaaggg ttataattat
     9841 aaattattac taattttgtt tgttttgttg agacggggtt ttactgttta gccctggcca
     9901 tcctgggact cactatgtaa actaggtggc cttaaactca caaagatcct ctgtctccca
     9961 agagctggga ttaaggccat ggaccagtat acctagccct taaatttttt tgggtgctgg
    10021 aggaatggct tagtggttaa gaacactgag tgctttccag aggttctgag ttcaattccc
    10081 agcaaccaca tgaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca
    10141 ggctggcgcc ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa
    10201 cttcgaaccc tttcccacac caccctccac acttgcccca aacactgcca actatgtagg
    10261 aggaaggggt tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt
    10321 tatgttcttg cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg
    10381 gggtagaagg tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag
    10441 ccaggtccct ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct
    10501 ggggtaccct attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg
    10561 gctcctcctg ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa
    10621 gggggacggg attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc
    10681 tctcacctgt ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa
    10741 caaatgcaaa cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg
    10801 gatcccctag tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac
    10861 acacacacac cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac
    10921 cacccccaat gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag
    10981 tatctgcaac ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc
    11041 aaccagtaac ctctgccctt tctcctccat gacaaccagg tcccaggtcc cgaaaaccaa
    11101 agaagaagaa ccctaacaaa gaggacaagc ggcctcgcac agccttcact gctgagcagc
    11161 tccagaggct caaggctgag tttcagacca acaggtacct gacagagcag cggcgccaga
    11221 gtctggcaca ggagctcggt acccggaaga tctggactct agagaattcc gcccctctcc
    11281 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
    11341 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
    11401 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
    11461 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
    11521 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
    11581 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
    11641 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
    11701 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
    11761 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
    11821 tttcctttga aaaacacgat gataagcttg ccacaaccat ggaagatccc gtcgttttac
    11881 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11941 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    12001 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
    12061 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
    12121 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
    12181 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
    12241 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
    12301 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
    12361 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga
    12421 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
    12481 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
    12541 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
    12601 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
    12661 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
    12721 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
    12781 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
    12841 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
    12901 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
    12961 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
    13021 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
    13081 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
    13141 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
    13201 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
    13261 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
    13321 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
    13381 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
    13441 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
    13501 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
    13561 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
    13621 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
    13681 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
    13741 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
    13801 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
    13861 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
    13921 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
    13981 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
    14041 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
    14101 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
    14161 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
    14221 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
    14281 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
    14341 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
    14401 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
    14461 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
    14521 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
    14581 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
    14641 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
    14701 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
    14761 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
    14821 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
    14881 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggggggatc
    14941 taagctctag ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg
    15001 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    15061 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    15121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    15181 tatcttatca tgtctggatc cggaataact tcgtatagca tacattatac gaagttatgt
    15241 ttctagcttg gctgcaggtc gtcgaaattc taccgggtag gggaggcgct tttcccaagg
    15301 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
    15361 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
    15421 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
    15481 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
    15541 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
    15601 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
    15661 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
    15721 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
    15781 agcctgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    15841 ggaactaaac catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
    15901 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    15961 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    16021 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
    16081 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    16141 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    16201 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    16261 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    16321 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    16381 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    16441 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    16501 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    16561 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    16621 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
    16681 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
    16741 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    16801 tctcatgctg gagttcttcg cccacccccc ggatctaagc tctagataag taatgatcat
    16861 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    16921 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    16981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    17041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgggg
    17101 gtaccgcgtc gagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
    17161 gtcgagataa cttcgtatag catacattat acgaagttat gtcgagatat ctagacccag
    17221 ctttcttgta caaagtggtt gatatctcta tagtcgcagt aggcggcttc tggtgtgtgt
    17281 ctgaagacaa tgatggtgtg ctcacataaa taaaataaat taatttttta aattaaggat
    17341 atttttcttc tttcctatcc caaacaaaca aacaaaattc ccctggggtg tggagagcaa
    17401 tctcattggt taagagcact agctactctt ccagaggacc caggatcaat tcccagtgcc
    17461 tacatggctg ctcaaaacct tttgataccc ctcacacata cataaatgca agcaaagcaa
    17521 caatgaaaat aaatcatttt tttaaaaata ttccccatca ctgccaattt cagccttttt
    17581 gtgaccttac gtgccactgg gctctggggc agcgcaggct aacgtccttg gtctcgcagg
    17641 gcttcttccg ccggacaatc cgcatgaagc tcgagtatga gaagtgcgat cggatctgca
    17701 agatccagaa gaagaaccgc aacaagtgtc agtactgccg cttccagaag tgcctggcac
    17761 tcggcatgtc gcacaacggt gagggcgcct gcgcagtctg tctcaggacc ccaaagggcg
    17821 cctgcgcagc acagctaacc cagggtccca ttctgtcaga gggcgcccag cacctgcccc
    17881 tggcacggat gggtgtgtgc acttgctgtt gatggctgat acaaatgcca ggacctcaga
    17941 ggcctgagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt
    18001 atggtctgag ctcgccatca gttcattctg cttcaagtga caagcccagc agggatgggg
    18061 ttgccaacac tgctccccac acttgtgcct gacagcaggc aaagctgagg ccagggttcc
    18121 ccggtcctga actgttgagg gtaggcagca gccaggaccc agttaggaat ttcttggtgg
    18181 ccagagactc gcaggagcca tccttgcctc aggcttccag agaactggga gcctcctgcc
    18241 attctctcct tggctgggag ctcctaggag tcacctctgt ttgcctcttc acccccagct
    18301 gtttcatatg agttcagtgg cctcaggtcc tcaaggtacc actccagccc gcacctctgc
    18361 ccagagtgaa tgcccctggt tgtcctagta agtgcaaagg atttccattt tccctgacct
    18421 acaaagacag ggaaggaaga aagccatata gccgggcgtg gtggtgcatg cctttaatcc
    18481 cagcactcga gaggcagagg caggcagatt tctgagttcg aggccagcct ggtctacaga
    18541 gtgagttcca ggatagccag ggctacacag atagaccctg tctcaggaaa aaaaaaaaaa
    18601 aaaaaagcca taaaatggaa aggtaaagag ccggagctca ggcatgggcc ctggtcacca
    18661 caggatcttt ctagttgcca tgcctggcca gcccccggcg gagtcagcaa aggctctcat
    18721 catggagctg cttccccgct gactgtgggg cctctggggg cccctggctc ctaaaggatg
    18781 atccccctca cctccacacc tgagaggagc aggggagagg aggggtgctg gcccggcccc
    18841 ccatgactgg ctcccacccc ctatgcagct atccgctttg gacggatgcc ggaggccgag
    18901 aagaggaagc tggtggcggg gctgactgcc agcgaggggt gccagcacaa cccccagctg
    18961 gccgacctga aggccttctc taagcacatc tacaacgcct acctgaaaaa cttcaacatg
    19021 accaaaaaga aggcccggag catcctcacc ggcaagtcca gccacaacgc agtgagtgtc
    19081 actggccagc ccagccatga ggtggggttc aggggacacg agggtggggt ccccgtcctt
    19141 ccccaggcct ggcctctgat gggcctggtt ttcagccctt tgtcatccac gacatcgaga
    19201 cactgtggca ggcagagaag ggcctggtgt ggaaacagct ggtgaacggg ctgccgccct
    19261 acaacgagat cagtgtgcac gtgttctacc gctgccagtc caccacagtg gagacagtcc
    19321 gagagctcac cgagttcgcc aagaacatcc ccaacttcag cagcctcttc ctcaatgacc
    19381 aggtgaccct cctcaagtat ggcgtgcacg aggccatctt tgccatgctg gcctccatcg
    19441 tcaacaaaga cgggctgctg gtggccaacg gcagtggctt cgtcacccac gagttcttgc
    19501 gaagtctccg caagcccttc agtgacatca ttgagcccaa gttcgagttt gctgtcaagt
    19561 tcaatgcgct ggagctcgat gacagtgacc tggcgctctt catcgcggcc atcattctgt
    19621 gtggaggtga gggggcggag ccgcactgcc agggaccaaa cctgacctca gacagtgtag
    19681 cacagagcac atgtgcagaa ccagctgcat ctggttggac agacagaggt gtaccttgct
    19741 gggattatag ctggatggcg ggatgcttgt ctagcaagcg ggaggcgctg agtccaatcc
    19801 ccagtactgg ggcgtgggga aaagaaatcc accatttgtg ggtagcggag aaatttctgt
    19861 gttactttca catttttcta gtccagtata atacacaggg aagcctgctc ccccacagga
    19921 gcagagaaca cacctcaggc cagaggtttt taactcattt ggtcctcaca gcaactgtgt
    19981 taaggacact gaagcccaga gccaccagga tgtccttcca cagagacagg ctcagaatcc
    20041 gggtgccagg tgtcctagct aggaatcatg cagccctata gatcaggaaa aacaactggt
    20101 cagaaacagg atgaatggag tccccagggg tggggttagc cacagagcaa gacggtccct
    20161 gcctgagatt actcataccc cgtgtcccca cagaccggcc aggcctcatg aatgtgcccc
    20221 aggtagaagc catccaggac accattctgc gggctctaga attccatctg caggtcaacc
    20281 accctgacag ccagtacctc ttccccaagc tgctgcagaa gatggcagac ctgcggcagc
    20341 tggtcactga gcatgcccag atgatgcagt ggctaaagaa gacggagagt gagaccttgc
    20401 tgcaccccct gctccaggaa atctacaagg acatgtacta aggccgcagc ccaggcctcc
    20461 cctcaggctc tgctgggccc agccacggac tgttcagagg accagccaca ggcactggca
    20521 gtcaagcagc tagagcctac tcacaacact ccagacacgt ggcccagact cttcccccaa
    20581 cacccccacc cccaccaacc cccccattcc cccaaccccc ctcccccacc ccgctctccc
    20641 catggcccgt ttcctgtttc tcctcagcac ctcctgttct tgctgtctcc ctagcgccct
    20701 tgctcccccc ctttgccttc cttctctagc atccccctcc tcccagtcct cacatttgtc
    20761 tgattcacag cagacagccc gttggtacgc tcaccagcag cctaaaagca gtggggcctg
    20821 tgctggccca gtcctgcctc tcctctctat ccccttcaaa gacatgagcc atccaaagaa
    20881 acactacgct ctctctgggc ccagctttcc aagaagcctg gcctggacca actgccatcc
    20941 cagcttgtgg tcaccaccac agggttcctc ctccagagag caagtgggca gggagcctgg
    21001 gccgggagcc atattcccag gctgtctcag ccctaggcac accactctct gacacttcct
    21061 ttcttctcgc cggcgtccta ggtcattgtc acagatgacc cttgtgctgc ctaggagatg
    21121 acccctccag atgtcccctc cagatgcggt ccaacggccc cactgaaggg aagggggtag
    21181 aggcaggccg gaaggagcag cggcacactt aggtcccagg gtcagaagct agacagcgag
    21241 tgggcaggcc ctccatcagc acccctcctc taccctgtag cagcatccag actggcagat
    21301 cccagtacca ggaactggac catagctgtt ctttcttctc ctgggagatg ctggcacacc
    21361 tgcccccccc ccccccttgc agctgccccg gtgtagccat gacactggct cacctctcgg
    21421 tcaccacaga gtccctccca ttccctcccc aaggccactg gggtacagct atggccctgt
    21481 tcttaggact ggtgatctgt gagcaggcag ggatatccta ccaggtcacc cctgccagct
    21541 cacaggcaga gttgctaggg ttcctctgac cctgtcctct ctcccactca cttgtaccag
    21601 tagctctgtg gccttctctt cttttgcctg gctggtcacc tgctcccatc tgctgcttca
    21661 agtggcttga aacttgctgg gtgctcccat actcagcccc agcccggcag atcctgcctc
    21721 taggcccata ggtgatcagc ccaggctcgg ctcctgccaa cacagaatgc tgccagattc
    21781 cccgctagca cactccctgc ccctcacctc tactgatcag gtcttggggt gttccttgtg
    21841 gggcccaccc aggctgagaa tggagctaca tcacccgccc tgcccccacc tgcccagccc
    21901 cgcccaggtc tggtgctgag gatgcagctc ctctcagggt ctgaagtctc caaatctgaa
    21961 atgtatattt ttgctaggag ccccagcttc ccgtgttttt aatataaata gtgtatacag
    22021 actgacggaa ctttaaataa atgggaatta cgtatttaag a
//