LOCUS       KO-first_condition_ready_41587_MGI:101865        21029 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Srms targeting project(s): 41587
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 86262
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9146..9267
                     /db_xref="ENSEMBL:ENSMUSE00000170786"
                     /rank="2"
                     /note="ENSMUSE00000170786"
     exon            5531..6344
                     /db_xref="ENSEMBL:ENSMUSE00000398460"
                     /rank="1"
                     /note="ENSMUSE00000398460"
     misc_feature    4275..9703
                     /note="5 arm"
     misc_feature    9731..9755
                     /note="B1 Gateway"
     misc_feature    9768..9815
                     /note="Frt"
     intron          9816..10690
                     /note="mouse En2 intron"
     misc_feature    10688..10690
                     /note="Splice Acceptor"
     exon            10691..10863
                     /note="mouse En2 exon"
     misc_feature    10875..11468
                     /note="ECMV IRES"
     gene            11469..14529
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14568..14811
                     /note="SV40 polyadenylation site"
     misc_feature    14815..14848
                     /note="loxP"
     promoter        14875..15394
                     /note="human beta actin promoter"
     gene            15420..16214
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16414..16655
                     /note="SV40 polyadenylation site"
     misc_feature    16672..16719
                     /note="Frt"
     misc_feature    16726..16759
                     /note="loxP"
     misc_feature    10705..10782
                     /note="FCHK std_gpr"
     misc_feature    16775..16795
                     /note="B2 Gateway"
     misc_feature    9704..16825
                     /note="Synthetic Cassette"
     exon            16984..17150
                     /db_xref="ENSEMBL:ENSMUSE00000170776"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000170776"
     misc_feature    16826..17302
                     /note="Critical Region"
     misc_feature    17326..17359
                     /note="Downstream LoxP"
     misc_feature    17303..17382
                     /note="synthetic loxP region"
     exon            19509..20327
                     /db_xref="ENSEMBL:ENSMUSE00000170796"
                     /rank="8"
                     /note="ENSMUSE00000170796"
     exon            19098..19254
                     /db_xref="ENSEMBL:ENSMUSE00000170793"
                     /rank="7"
                     /note="ENSMUSE00000170793"
     exon            18835..19016
                     /db_xref="ENSEMBL:ENSMUSE00000170795"
                     /rank="6"
                     /note="ENSMUSE00000170795"
     exon            18112..18270
                     /db_xref="ENSEMBL:ENSMUSE00000170788"
                     /rank="5"
                     /note="ENSMUSE00000170788"
     exon            17884..18025
                     /db_xref="ENSEMBL:ENSMUSE00000170781"
                     /rank="4"
                     /note="ENSMUSE00000170781"
     misc_feature    17383..21029
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagatag ctgaagaata atttctcacc agggaactac actgttaata
     4321 atgaggactt ggggataaaa atctccattt atacctgtca agggaagtat agacaaattc
     4381 tccacaaggc tggtcggtgt gagattcggg gttccttcca ttacttctct aattttatat
     4441 ttataattac cttttctaca cacccacttc caacatgcct gtgacatgga ccttgtacct
     4501 tagggctcct tgtcccttcc tggctgctca agccagggtc tgggattcag ccatttggga
     4561 tgttgggagg acgaagccaa tgagttctag gtatctctgg tacaccagaa cttacaaacg
     4621 ccccagatgc ttctctgaag catctgtaga tggagaagga ataggaatca tacctgtttg
     4681 atatgtttga agtataaaca gtacatgaag ggtctgtagc aaatacatat ggaagcttag
     4741 tagtatccac tcaggtgctt aaggtgctgt gcttgggaaa ggaaagacag caaatgggca
     4801 gaaagcctgc aagtgaggtc ccacctcaga ttcaaacatt cccaagaact gagctggctt
     4861 gaacctggtc tcccttacct gacagtgaaa aattgggtgg caggcagctt gcttcattgc
     4921 acagaataag aatcctggac tgggtaagag gctatctgag gctcgcccac tgcccttcct
     4981 ttgcatgctg atccccagtc atatacccac gctgaggtct tagatgtctc agtttccctg
     5041 cagtggtatt aagcatctcc tggttcagaa atcgtacccc atgattacct atgtacctgg
     5101 accttcagtg attcccctag agattactac cagcatctca ttttacaaca caggaatcag
     5161 gatagcaaga gtttactggg cgctgagcgt ttgctccaga ggcctccgct gagttggaca
     5221 ggctgcacat agctacttgc atctggccct gaaagcgggt gggagatggt gttgtgttat
     5281 gttaaagagc caccatttaa aaagtcagtg tttcacagaa atatgacctc tggcgatgaa
     5341 ctgctccctt tgttgacccc tctcccaagg cggctgctag ttgcacgtgc ccagctagcc
     5401 cggcaaagca ggtccctgca acctgcgtct gttagtgggg atgaaaaggg tagcttgttc
     5461 cagaaggccc tcccccctac accgcccttc ccgcagactc cgcccaactc tctctcaccc
     5521 tagtagccta ctgggacacg aatctcaagc ccagcggctt ttaaccccag cagagatcca
     5581 gctcaccaga ccgcacagct gtagaagagt atgtagacta ggaggagcag cccgtgtggt
     5641 cctgggacgg aggtcaccgt ccgccccacg cattgataca ccatccggga aactgactgt
     5701 tgtatgcctg attaaggagc actctgtagt gacctccggg gttagggtta gggtccgtgt
     5761 cacctgtcgc ttcccattct gtaccaaata gggacgtggg cagaaagccc ggggtaagcc
     5821 tggctgtccc accagccttt ccggtttctc tcttgcaccc tgggatcctg gtgcatggtt
     5881 gtagtgctca gcttcttcag cggcagcacg gaggtcccag agtactgacc cgaggtcggc
     5941 tccatgcgct ttcgtccgca accaggcccc ctaggcatgg agcccttcct caggaagcgg
     6001 ctcactttct tgtccttttt ctgggataag atatggccag cggatgaatc ggaggaagac
     6061 atccccagga tccagggaca cgacgacaac ccagtgccgg agcaagccgc tgccgttgaa
     6121 ccttgtagct tcccagcccc acgcgcccga ctcttccgcg cgctctacga cttcactgct
     6181 cgatgtgcag aggaactgag cgtcagccgt ggggacagac tctacgccct caaggaggag
     6241 ggggactaca tctttgccca aaggctctct ggtccaccca gcaccggact agttcctgtc
     6301 acctaccttg ccaaggctac cccggagccg ccctcagacc aaccgtaagt acctgggctt
     6361 gggaggtagg gctgccaagc ccaatgagta aatcggagtt ttgagagtag gcaatacctg
     6421 cttacagtca gtgagggctt attgagtgcc tgctggtacc gggctgagca gacaggtgtg
     6481 cccgggcagt acaaaaggaa gagttgcaca ggacctgctc acacccagga gtgagcaagt
     6541 cctgggaacc agttgttggc tagaacaatt actccccata gtcactgcag gaccctgccg
     6601 ccatacggag gttgcagtga gtggtttacc ttgcttccag accaggagtt gaggactgag
     6661 atgccctgcc aggtgataca gccttgcact gtttgggaag ctggaggggg agggggcaca
     6721 cacctgagcc tccaggtggc tctgggtccc cagtacttgt agatgctgca aaccagatca
     6781 gagccaagta ttcacatgct cctctttatc tatttatatt cacccaaaca cctcaaatgg
     6841 gtttaagtgt aaggcagata tagccctgat ttgctttgct gagtgggtat ggggaagagc
     6901 ttactctttg ccccccaagg ctgggcagtc actgccagtg agtggcatac cagagggtca
     6961 ctcagcaggg ttcatgctgg gcaaaaataa gctgaaagct gaagagcttg tctaccaaac
     7021 tgtccctggc cttacaccca actggtcctg gcctgagtcc tcaggtccta cctggcattg
     7081 ctcaaattca tctctagcct cctgaggatc cctcagcagt tacaacctcc caggagagtt
     7141 agtgtggccg gtgaagtaga gtcacaggaa agagagccag acacaagccc tgtgcctgtt
     7201 tgctggcccc cagcagtgag taggactaag ccaagaagct gttttaatgg aggggtctct
     7261 cagttaagtt atgggagaca gaacataaga acagtcctgt tcacggaaca ggcagcatgg
     7321 aaggcagggc tgggctcctg gaagtagggt tgctaatgga gagctaatga ctgcctgacc
     7381 tagagatcaa agagctgacc acacttatgg tatcattagt accagacacc tggccccatc
     7441 atctagggag agacccctga ggcacatcct ccactgttca ggctgtcaca ggagtccagc
     7501 tgtatcccat agtggcttgc accttgtcat agcttcaagg aaaagctggg gttatgctgg
     7561 agaaactccc acttctggtt cacccccact ccccatcccc acacagtttg actctgctga
     7621 gaagggatct cacccctagg gactcttgac ctgccagatc ttctcagttc cctgcccacc
     7681 aattgtgtag gatccagctt ctcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7741 aggagggtct ctagctctca gtagagagat aagaagcctc aagggactag acctacagct
     7801 tctgaagtga gtttgtggcc tccctaagca tgctgcctag cccaggcatc tgtttccacg
     7861 acagtccctc acatccattc agctggccag tgttaactcc catttctcag gcctcggcct
     7921 ccaccccctt acttgctcat ctttctgcct cagctgtgtt catccctcca gccctagcct
     7981 aggcccacac tctggccatg cgggttcctc cgctgtacat ctgaggggtg gggatgggag
     8041 actggcctgt ctgttctttg tgggttaacc tgaatcatgc cgagtatctc tcactacctt
     8101 gcctccatcc gaagagactg agggactaag ctcacacgtc ttttcatgca ttgctgtggt
     8161 tcccaaccca ctggttcctg aaatctctca gaagtggaaa agcttggatg tgaagttgag
     8221 ccagctatgg aaccttgtct gggccttgga aagcagagat gcattcaata gggtgggtct
     8281 gggactgact ttatctttcc gaggttgctt ctgcctcctg agaatgagga ggccaggcat
     8341 ggtggtatat gcttttaatc ccagcacttg gaggctgaag caggtggatc tctgagtttg
     8401 aggctagcct tgtctatgta gtggccatcc aagactacat actgagatcc tatctgaaag
     8461 aaagaaagga agaaagaaag aaagaaagga aggaaggaag gaaggaagaa agaaagaaag
     8521 aaagaaagaa agaaagaaag aaagaaagaa agaaagagaa agaaaggaag gaagaagaaa
     8581 aagaaaaata taagtgaatg gagccatacc tgaggtacct gatgtgactt agaactcagg
     8641 ggctggccca cctaggcatg gcccttcaga gggcacaagg tacattgcca ccccccagct
     8701 tctactatct gcctctgcta acatcctgac caccctggct tagtctccct cttctggggc
     8761 tcccagctcc caagtagcca acctttagat gagccatacc cagccaggca acacctaggg
     8821 tctctggcca tactagagtt ctatgggtat ttctagggca gcttattgga cccccacagc
     8881 aattaggcag tttcaagttt ggggttttgg tctttggtga ggcctggtag ttatgttaga
     8941 gtaatagatc cagtggccta ggtgagtgga cagtcatacc tgtatgaagg agcttccagt
     9001 cacctgatag tggcggcctg ggacctcagc ttcaggcact gggcacattt ccttcctgag
     9061 agacagcaga gaagttgatg cttcacacct tgccagggtc acatggctgg gcctgaaaaa
     9121 tgacttttgt gtcctggatt tgcagttggt acttcagtgg gatcagcagg gctcaggccc
     9181 agcagttgct cttgtctcct gccaatgcac caggggcctt cctcatccgg cccagcgaaa
     9241 gcagcatcgg gggctattct ctatcaggta cttgaacaac ccctcaagac ttcatttact
     9301 ggaggggggc atcttgctca tctgtcccag gccttggggc agttgattcc cacactagca
     9361 gcctttcata tagacatgct agttattaag taccctccct tcctcccttc cttccctcct
     9421 tcccttcccc cttttttcct tcattcttcc ctccctctct tccttcttct ttccctccct
     9481 ccctgcttcc ctcctttcct ctttcctccc tatcctcctc tcttcttcct tcctcccctc
     9541 cattcctccc tcccttcctt ccttccttgc ttcttctttc tttctttctt tctttctttc
     9601 tttctttctt tctttctttc tttctttccc cacttcctcc attttctacc tagttgagtt
     9661 agcattccat tcaacactaa cctatttaac actccttgtc cccaaggcgc ataacgatac
     9721 cacgatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa gttcctattc
     9781 cgaagttcct attctctaga aagtatagga acttcgaacc ctttcccaca ccaccctcca
     9841 cacttgcccc aaacactgcc aactatgtag gaggaagggg ttgggactaa cagaagaacc
     9901 cgttgtgggg aagctgttgg gagggtcact ttatgttctt gcccaaggtc agttgggtgg
     9961 cctgcttctg atgaggtggt cccaaggtct ggggtagaag gtgagaggga caggccacca
    10021 aggtcagccc ccccccccta tcccatagga gccaggtccc tctcctggac aggaagactg
    10081 aaggggagat gccagagact cagtgaagcc tggggtaccc tattggagtc cttcaaggaa
    10141 acaaacttgg cctcaccagg cctcagcctt ggctcctcct gggaactcta ctgcccttgg
    10201 gatccccttg tagttgtggg ttacatagga agggggacgg gattcccctt gactggctag
    10261 cctactcttt tcttcagtct tctccatctc ctctcacctg tctctcgacc ctttccctag
    10321 gatagacttg gaaaaagata aggggagaaa acaaatgcaa acgaggccag aaagattttg
    10381 gctgggcatt ccttccgcta gcttttattg ggatccccta gtttgtgata ggccttttag
    10441 ctacatctgc caatccatct cattttcaca cacacacaca ccactttcct tctggtcagt
    10501 gggcacatgt ccagcctcaa gtttatatca ccacccccaa tgcccaacac ttgtatggcc
    10561 ttgggcgggt catccccccc cccaccccca gtatctgcaa cctcaagcta gcttgggtgc
    10621 gttggttgtg gataagtagc tagactccag caaccagtaa cctctgccct ttctcctcca
    10681 tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga accctaacaa agaggacaag
    10741 cggcctcgca cagccttcac tgctgagcag ctccagaggc tcaaggctga gtttcagacc
    10801 aacaggtacc tgacagagca gcggcgccag agtctggcac aggagctcgg tacccggaag
    10861 atctggactc tagagaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa
    10921 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
    10981 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
    11041 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
    11101 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
    11161 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
    11221 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
    11281 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
    11341 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
    11401 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
    11461 ccacaaccat ggaagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    11521 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    11581 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct
    11641 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg
    11701 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca
    11761 acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt
    11821 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
    11881 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
    11941 gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa
    12001 accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata
    12061 tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa
    12121 tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg
    12181 ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc
    12241 agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc
    12301 gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga
    12361 gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc
    12421 tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc
    12481 tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc
    12541 tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga
    12601 acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt
    12661 gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc
    12721 caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc
    12781 gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat
    12841 caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc
    12901 gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga
    12961 tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa
    13021 aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga
    13081 tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt
    13141 tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg
    13201 gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct
    13261 gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac
    13321 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
    13381 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
    13441 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
    13501 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
    13561 aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc
    13621 tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca
    13681 ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt
    13741 caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg
    13801 atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg
    13861 accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt
    13921 tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt
    13981 ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg
    14041 gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga
    14101 ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc
    14161 cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat
    14221 tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc
    14281 gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct
    14341 acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca
    14401 catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt
    14461 cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
    14521 aaaaataata ataaccgggc aggggggatc taagctctag ataagtaatg atcataatca
    14581 gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    14641 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    14701 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    14761 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggaataact
    14821 tcgtatagca tacattatac gaagttatgt ttaaacggcg cgccccggaa ttcgccttct
    14881 gcaggagcgt acagaaccca gggccctggc acccgtgcag accctggccc accccacctg
    14941 ggcgctcagt gcccaagaga tgtccacacc taggatgtcc cgcggtgggt ggggggcccg
    15001 agagacgggc aggccggggg caggcctggc catgcggggc cgaaccgggc actgcccagc
    15061 gtgggcgcgg gggccacggc gcgcgccccc agcccccggg cccagcaccc caaggcggcc
    15121 aacgccaaaa ctctccctcc tcctcttcct caatctcgct ctcgctcttt ttttttttcg
    15181 caaaaggagg ggagaggggg taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag
    15241 cggcgcgggg ccaatcagcg tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc
    15301 gcggcggcgc cctataaaac ccagcggcgc gacgcgccac caccgccgag accgcgtccg
    15361 ccccgcgagc acagagcctc gcctttgccg atcctctaga gtcgagatcc gccgccacca
    15421 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
    15481 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
    15541 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
    15601 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
    15661 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
    15721 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
    15781 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
    15841 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
    15901 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
    15961 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
    16021 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
    16081 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
    16141 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
    16201 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
    16261 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    16321 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    16381 ccaccccccg gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt
    16441 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    16501 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    16561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    16621 caaactcatc aatgtatctt atcatgtctg gatccggggg taccgcgtcg agaagttcct
    16681 attccgaagt tcctattctc tagaaagtat aggaacttcg tcgagataac ttcgtatagc
    16741 atacattata cgaagttatg tcgagatatc tagacccagc tttcttgtac aaagtggttg
    16801 atatctctat agtcgcagta ggcggtgtcc tggggtctgt agcttgaccg tgccccctct
    16861 tgcatagact attgtccacc tcagcgaccc catggtcaat ctgagagaag ccacttgata
    16921 tgctggggtg tgggtgtctg aagcaggtgt agccaaggca ctagtttcca tctctgtctc
    16981 cagtcagggc ccaggccaaa gtctgccact accgcatctg catggcaccc agtggcagcc
    17041 tctatctgca ggagggccaa ctcttcccca gcctggatgc actgctggct tactacaaga
    17101 ccaactggaa gctgatccag aaccctctgc tgcagccctg cataccccag gtggacctga
    17161 gactttcccc atacctgggg accactggga accctgtttc agggtatctt ggcaagcctc
    17221 tggcctaggc ttgcccggga atagccagtg tccagtgatt tctctattcc gatggagttg
    17281 gcccccttcg gcggttttat ctgagatggc gcaacgcaat taatgataac ttcgtatagc
    17341 atacattata cgaagttatg gtctgagctc gccatcagtt caaaaactgg ggaccttcaa
    17401 ggggtatagt gtgcacccaa tctgcaggac atcaggagga gttatggcca gtgtggggaa
    17461 ggcacccaca gctgctccaa gccctgaggg cagaagctga gagtagagat actatttccc
    17521 ccagtatctg gtattactgg gtccaggaca gcgatctggt ttctctggtt ctgtaatgac
    17581 agcctagcca gggttgtaat gagtgtttgc aaacggatgt ccggttctgg taaaggggcc
    17641 gcttaagttc ccaaagcaat tagcttccta ctctgagctt ggcctcctgg aggtctgcac
    17701 tggtctcccc tttgtcctag cctgggcctg gagtaggggt gcactgggga gggagaggca
    17761 gaggcctgat gctggggctt ttcctgagac tcacagggag gctaggttca aatattagct
    17821 ctatcttcca gcccactctg ctaggggggt ctaaagcagc acactgaatg tcccttccca
    17881 cagataccct tggttcagga cgagtgggaa cgaccacgtt cagaatttgt ccttcggaga
    17941 aagctgggtg aaggtttctt cggggaggtg tgggaaggcc tgtggctggg ctctatccct
    18001 gtggcagtga aggttatcaa atcaggtgag gctctgtagc attgccctct gaccctggag
    18061 cctggggcag tggtggccat gcacagtcct ggaacaacat ccttttcaca gctgacatga
    18121 agctggcaga cctcaccaag gagattgagg cactgaagag cttgaggcat gagaggctga
    18181 tccggctgca cgctatatgt tccctcggtg aacctgtgta catcgttact gaactcatgg
    18241 gcaagggcaa cttgcaagtc tacctgggca gtgagtaacc tctactcggg cagtgaatac
    18301 tctccctatc cctaccctgt agcagccaca tctgccaacc cttacaatag catccatgcc
    18361 ttgggtctac aaagaagaat ccttaggcct ttgtgagaga cacatgtggc ctaggttgca
    18421 gatacttggt cctaatgacc taatgaggcc aaggatctgg tccaggccta gatttggcta
    18481 aaggctatgt agtcaaatcc tgttagttct gaggctagga ctgccataca gtggtagagg
    18541 ttccctttgt gtcactgacc tctgatgcgt tgccaggttg ggtagtatct cccttggggt
    18601 tacaagcagg tagcaagaag caggttgtgc ttgaggcctt gtggtgactt ccctttccat
    18661 gttattcatt gttgactggg ccttagtagg tgttgtgctc cctccctcag agatggggga
    18721 tcagaggcac agatgaagca tgtgtatgca tgtgtacttg cctgacccgc cattatcctg
    18781 tgtccttatg aggactgcca cccatccctg atccctgagc ccctctctct gcaggctctg
    18841 agggaaaggc cctgagcctg ccccatctac tgggatttgc ctgccaggta gctgagggca
    18901 tgagctacct ggaggagcgg cgtgtcgtcc accgggactt ggctgccagg aacgtgctgg
    18961 tgggtgatga cctcacctgc aaggtagctg attttggcct ggccagactg ctcaaggtca
    19021 gtgaggctct gggctcctaa tccctgcata gaggaaaata ctactctgaa ctcccaacca
    19081 ttatacctca tccacaggat gatgtctact ccccaagcag tggctccaag atccctgtca
    19141 agtggacggc acctgaggct gctaattacc gtgtcttttc ccaaaagtca gatgtctggt
    19201 cctttggcat cctgctgtat gaggtcttca cttatggcca gtgtccctat gaaggtaagc
    19261 catctgagaa gagataaaca agatcatgac tcatcaggga aagtttagtc tgagggaggt
    19321 agcctggaat ccagtctgag ggaggagatg taccctggga gtcactgacc cccaaaggta
    19381 ccagcatagg ggctggagta ccagcacaga ctggccatgt agaggctggg ggtggggtag
    19441 tctctttggt ggcacctagg caggatcaag gcagccgctg actggtcctc ttgctggccc
    19501 cttatcagga atgaccaacc atgagacgct acagcagatt agtcgtggat accggctgcc
    19561 acgcccagct gtctgcccag cagaggtcta tgtgctcatg gtagagtgct ggaagggcag
    19621 ccctgaggag cgtcccacct ttgccatact gagggagaag ctgaatgcca taaacagacg
    19681 cctccatctg ggcctcacgt gaccaggtct ccagaccctc agtagcagct agggctgccg
    19741 cttcctgaag ggtgtctccc aaggaaacat ttgtcaccag tgcccatggg aaatactgca
    19801 caactgagaa tgataggagc ctctgggctc caacactgcg gctccttcac tgacacaggc
    19861 tggtgcctac atatgcacac cagagagact catgcaacta catgtgcaca ccagagagac
    19921 tcatgtacct acatgtgcac atcggatgga tgtgttcaga ctggtcctgg tcatgagcac
    19981 ttacacagat atgaaggctg atgccctcct cctggcacag tctccaaagt gttgataaag
    20041 catagaggag gagccgcaag gatgtgcttt gacagacgag agccagcagt ttcctccttg
    20101 ccctcacctc agctccctcc tagaaccaag gatgaaaaag ctcactttcc ccaggtcttg
    20161 ttgtggattg ggttaagtag ccgatactag ggctggctgt gggccattac atgctatgta
    20221 cttttagggg acagcagcat cacctgcccc tacccagtgc ccacaggtac cagtaaacct
    20281 taggaacaca aagtcagctt caataaacat gttctggtgg tttcacattc tggttcccag
    20341 aatgcaatgc tggaggctct ggaacacggt ggagagagag agagagagaa gacaccgagg
    20401 cattcatggt gaacactgct agatctaagt gagcagtcct ttccccctcc ctggcctgga
    20461 attgaagcca gaagactctt cagcttcagg aagggcagac tggctatttg gtagtcagca
    20521 cgcccaggcc ctttctagat gcagaggaat gttgttttga gaatatctct ttgggtgata
    20581 ggcagtggtt agaaggcaga cttttctggt tggtgggatt aggcagatat agggtatcta
    20641 gtcagccaaa gggagggtgt tggcaaccag atcctctctg gggcaaagcc cccatggacc
    20701 actttttaag ggtaacacct tgaggagttg ggcctcagat cactcaacac ttcagggtac
    20761 ttaggggcat caatcttagg gggcagggtg atgggggctt tgctcaggga cccaagtcca
    20821 tttccctttc ctaccagttg tacccagaag gttgtggggc agagagactg gcagtttcta
    20881 cagtggatcg aatttggggc cagctttgca ctcaccaagg ccaattttct catgattact
    20941 ggccacattt gtatattagc taagaaggat ctggaaactg aacctcatgt gtaaagtgac
    21001 cccttagcta gaacaaagat tttgggcat
//