LOCUS       KO-first_condition_ready_47692_MGI:101863        23317 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Aldoc targeting project(s): 47692
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 96091
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8527..8781
                     /db_xref="ENSEMBL:ENSMUSE00000825326"
                     /rank="1"
                     /note="ENSMUSE00000825326"
     misc_feature    4275..9814
                     /note="5 arm"
     misc_feature    9842..9866
                     /note="B1 Gateway"
     misc_feature    9879..9926
                     /note="Frt"
     intron          9927..10801
                     /note="mouse En2 intron"
     misc_feature    10799..10801
                     /note="Splice Acceptor"
     exon            10802..10974
                     /note="mouse En2 exon"
     misc_feature    10986..11579
                     /note="ECMV IRES"
     gene            11580..14640
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14679..14922
                     /note="SV40 polyadenylation site"
     misc_feature    14926..14959
                     /note="loxP"
     promoter        14986..15505
                     /note="human beta actin promoter"
     gene            15531..16325
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16525..16766
                     /note="SV40 polyadenylation site"
     misc_feature    16783..16830
                     /note="Frt"
     misc_feature    16837..16870
                     /note="loxP"
     misc_feature    10816..10893
                     /note="FCHK std_gpr"
     misc_feature    16886..16906
                     /note="B2 Gateway"
     misc_feature    9815..16936
                     /note="Synthetic Cassette"
     exon            17001..17124
                     /db_xref="ENSEMBL:ENSMUSE00000661700"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000661700"
     exon            17215..17426
                     /db_xref="ENSEMBL:ENSMUSE00001238533"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00001238533"
     exon            17520..17574
                     /db_xref="ENSEMBL:ENSMUSE00001260473"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 3 ENSMUSE00001260473"
     exon            17660..17820
                     /db_xref="ENSEMBL:ENSMUSE00000109906"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 4 ENSMUSE00000109906"
     exon            17940..18023
                     /db_xref="ENSEMBL:ENSMUSE00001312558"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 5 ENSMUSE00001312558"
     exon            18253..18427
                     /db_xref="ENSEMBL:ENSMUSE00001231974"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 6 ENSMUSE00001231974"
     exon            18546..18745
                     /db_xref="ENSEMBL:ENSMUSE00000286043"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 7 ENSMUSE00000286043"
     exon            18873..19070
                     /db_xref="ENSEMBL:ENSMUSE00000826116"
                     /type="targeted"
                     /rank="9"
                     /note="target exon 8 ENSMUSE00000826116 5' fragment"
     misc_feature    16937..19070
                     /note="Critical Region"
     misc_feature    19094..19127
                     /note="Downstream LoxP"
     misc_feature    19071..19150
                     /note="synthetic loxP region"
     exon            19151..20357
                     /db_xref="ENSEMBL:ENSMUSE00000826116"
                     /rank="9"
                     /note="ENSMUSE00000826116 3' fragment"
     misc_feature    19151..23317
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaatatg tatgtgaggt ccaagagtta aggaaagact tgtgagttga
     4321 gaaaagaaac ttctccaagg cctgtctatg aggccttgat catgatccag aagaaatggt
     4381 ttcccaaagc ctgaccatca ttgtcaaaca gtaaagtaag ggctagaaag tacctgttgt
     4441 gtttgaaagt aaggatgcca ggcctggaag agggtttagt gattaagagc acatactgtc
     4501 cttgaagagg acctgagttc agttcccagc acccacatct gatagccaca gctgtctgta
     4561 acgccagtct caggccgtct gacatcctct gtcctcactc atatgtacac atacacatac
     4621 ataaatgcta agaaagaaag aaagaaagga aggaaggaag gaaggaagga aggaaggatg
     4681 gatggatgga tggatggatg tcagtggcgc actactgaca aggtagtaag gtaagaatta
     4741 taggcctgcc tgccttcctc cctcctcatt aaatagaaga gaaaggacag taggcactaa
     4801 aacatttaag ggcttttctt ttaattactg ttgttttagt gggtgagact taaacacata
     4861 agaatgttca gggaaaggca ttcatgggga gctagagaga gaactgctgt aaggtagatc
     4921 gtggatgctc accctggaag gacaattcag agctcaggaa atgatctaaa gttgtaaagt
     4981 acagcggaac agtggatggt tatttttttt ttcctttgtc cccaggctga atcagagatc
     5041 attctgccca gcacaggctc tgctccagcc caggaacacc ctctgtccaa tgacagcagc
     5101 atctcagaac agaccccgac agcagcagta gatgaaggtt aacgaccttg gcatctcagg
     5161 gagcggaaag tgacctgctc ttgctctcag cagtacagat gcacactgcc agacatgctg
     5221 gtcttacccc tctctatggt gttgggtttt tgtttttgtg tttatttaag aggtagggtc
     5281 ggtgctggag agatggctca gaggttaaga gtactgactg ttcttccaga ggtcctgagt
     5341 tcaaatccca gcaaccacat ggtggctcac aaccatctgt aatgggatct gatgccctct
     5401 tctggtgttt ctgaggacag ctacagtgta cttatatata ataaatgaat cttaaagaaa
     5461 aaaaaaaaaa aagaggtctc atgtggttca ggatggccat gaatttctat gtggctaaat
     5521 aaccctgatt tgtttttgct ttagtgccag aaccagctcc tgtgccattg cttggaagtg
     5581 ttaagagtgc tttcacccga gtagcctcaa tggcttcctt tcagcctaca ggttaggatg
     5641 ggctccaggg accatccctc atttctcaaa ggtcaggtag tgatgtgcaa agacaaaagc
     5701 agtcagaagt gctttgctct aaaaacctca attctacttg ggctcatttt atttctgtct
     5761 cttgggatct gtaatgttcc ggatagttaa accatgtggg tgggaaacat aaagttgtgg
     5821 gagcagtggt tgtgtaccct gtccttgttg gcagaagggc tatgtctcca tctagaactt
     5881 tgtctttcag agaccccaga cttggagaag agcctggcag aaatgagtac tgtgttacaa
     5941 gagcttaaga gcctgtgttc cctgctgcaa gagtctaaag aggaggccac tggggtcctg
     6001 cagagggaaa tgtaagcggt gtcagtgtta atgatgggga gtgatgagag cagccctcat
     6061 gccaagggca gagcatgaga atgatgccag attctcctgc cccccctcac cccccccagc
     6121 tgtgaactac actcgagact acaggcccaa gaagaagagc atcaggaagc cctgaaggca
     6181 aaggaagcag acatggagaa gctgaaccag gccttgtgct tgctccgcaa ggtgaaggag
     6241 ggaccttaaa gtggggcaat gaggaactgg ggcagctccg ctggcccatt gggaccccct
     6301 ttctaggaat ctaggtagtg ggggggttcc aactactgct ctagttttgc ccttttgggg
     6361 tcagacactt cctatctgag aactagtact gggttgacct gtcttctcca agcagaccca
     6421 ggagaaaagc ccagaactga agcagagttc tgaaaccaag acgtgccatg cttgacttgg
     6481 tcctgaataa gtggacggat atgaacgagt ccccggggct gagcatggct cgggcattca
     6541 gtcctgtctt ctggctgaaa agaagtgagc ctttcctcag tgcttctgtc cttcttcaca
     6601 gaatgagaag gagctcctgg aagtgataca gaagcagaac gagaagatcc tggggcaaat
     6661 agacaagagc ggccagctca tagtacgttt ctcctgatgc tatggccagg tctctgcttt
     6721 tcccacctga tggaggaagg agagaaagaa cctctgagta ctgttgggtt atctccgtta
     6781 cccctgagtg ctgttgggtt acctctgctc ttgttcccag aacctcagag aggaggtgac
     6841 ccagctcaca cagtcacttc ggcgtgcaga gacagagact aaagtgctcc aggaagccct
     6901 ggaaggccag ctagatccca gctgccagct gatggctact aactggatcc aggaaaaagt
     6961 gtttctctca caggaggtaa cacagagtta acaaaggagc tgtacaaata atggggaccc
     7021 cacgctatgc ccctattaac gtggacatat ttataggtga gcaagctgag ggttatgttc
     7081 ctggagatga aaactgagaa ggaacagctg atggacaagt atctgagcca tgtaagcagt
     7141 tctgtttcct tccaccacag aagcaccctg ccatatatat ttcctctgtt ctctctagct
     7201 gtgttcttta cgtgtagggt tcatgaagcc tgtttgcatt gtagaggcac atcctggagg
     7261 agaatcttcg gcgctctgac acagagttaa agaaactcga tgacacaatt cagcatgtct
     7321 atgaggtaaa agtgaaggag gtagtgggac ctggctgccg gaggggctga ggagcatggc
     7381 tccggttctc agcctgacta cttttttttt tttttaaaga tttatttatt tattatatga
     7441 aagtacactg tagctgtcct ctgagggagt cagatctcat tacagatggt tgtgagccac
     7501 catgtggctg ctgggacttg aactcaggat cttcggaaga acaggcagtg ctcctaaccg
     7561 ctgagccatc tctccagccc tcagcctgac ttcttacagt ggcttcattt tcacttttcc
     7621 agcatcacag gccaggagct ttggcttcag gcttgtgatt cagaactgtt ttctgtctct
     7681 tcccagactc tgttgtctat cccagagact atgaagagtt gcaaggagtt acaaggattg
     7741 ctagaatttc tgagctaaga aactgaaaat attggaatgc agtggtgtgc acctgtagtt
     7801 ccagctgctc cagacgccaa agttggagtg ctgccaaatc ccaggaattt gggaacctaa
     7861 cacagacaac acagcaaaaa ccctgaaaaa tgaattccac tccactccag tttccttcag
     7921 ggctctttat cccaaccctt atcccaataa taagaccaat tggcatagag ccgaccatgt
     7981 tttatgttat ttaaataaag tgtatttaat gtagtccttc cagtccactg aatcttattt
     8041 tgggggtagg agtgggcaga tcagcacttc agcctaggca cccttcacca cttcctgctg
     8101 cagctaaagc caaggctgac tgcttttctt tgcttgattc tcttcttcta ccaacaaaaa
     8161 aagggggtgg gggggagggc aatggactgc ctgttccctg gaccactgcc tggggggaac
     8221 agaaaagact ccattcactt actttcccca tcccccttgg gggtaggcac tggtaaatat
     8281 ggggcagagc acagaaactc aattccatta ttccatgtct tgaagtgggg cactgttagg
     8341 tgggcctctg ccctacctgc tgatgtttag agatctctcc tctaaaccag gcttaatccc
     8401 caacattcca cataatgcct tacacagcaa aggactgatt gtggtagaga caaacacaag
     8461 tccatctctt ccctctaggg ccctgttggc agggttccat gatgtgggaa gggcatcgtc
     8521 tctaacaacc ttccggaccc tggcagagga atcactccac ctcactactc cacctctgag
     8581 ggcgtggcct taccctagac cagtcctggg gagagggcgg gaccaggacg ggaggtatct
     8641 gtcacgcccc cagggagtca cgtagctctg cggcaagtgc tgccacctta tttactccag
     8701 cttggaccga gctaatcaga ggaaaagtga gctgtgctgt ggctcccgcg gctgactggc
     8761 tgagtgactg gctgagtata ggtaaggtct ccaagcgagc ctgagatgct cgcgtccggt
     8821 ttgtcttccc atgctgtcct tgccctctcc ctgggaccca agttcctctg ctttctgtgg
     8881 ttttgaaacc ccatgcatgg ctcaggaaaa ccgtgcttct cttcttcctt ctagttctcc
     8941 tttccagttc caagtcatat gcctccagga ctttttcatt ggggtcagtg cattccaccc
     9001 aggacatttt aatctcagtc catttctggt tcttctaggc ttgtcctcta ctctagggta
     9061 taggcccagg aagagcatca ggaccggtta gcttgtatca ccattacccg tctcacctct
     9121 tccaggtctc cttcctgctt ttcctgcatc tctccctctt tcccataatc cactcactca
     9181 gccttaattc ctcgcctctg gggtctctac taggaggctg agttgtgggg agccaagcct
     9241 ccctatttcc tgtgcctctt gcatatgagg gaatgccggg tactagggat gatctggtga
     9301 tgtctctttc ctgtcctctc atgtatgctg atattgcctc tctcctacca tacttgcagc
     9361 cctgggagtc aatctccacc ataggtaggg gagtaggcag tagcaaaggc agtcaggttt
     9421 tcagtacctg taccactgct actccctgcc ccctgccttc cactagactt ggggaaggat
     9481 gctgtaggtt aaacgtctca ttcacaaacc tttgagaaaa gtgatccccc cccccccccg
     9541 ccttagggac ccttttgaag aaaagaaagg ggaagcagga aagcaaaaat cagaaacaac
     9601 aggttttctg tttacctcag gaaggggcca gattcagtga aagaagagct ggcattctca
     9661 acctggcagc agtttccagg aagacctgtg gcttcagggc tgtcgcctcc catcccccac
     9721 gctgcatctc ttctggagct gggaagggtg cccaccagga acaaaaggag atgtgggaac
     9781 tggggcccta actctgttag ctgtcaaaga gattaaggcg cataacgata ccacgatatc
     9841 aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt ccgaagttcc
     9901 tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc acacttgccc
     9961 caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac ccgttgtggg
    10021 gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg gcctgcttct
    10081 gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc aaggtcagcc
    10141 ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact gaaggggaga
    10201 tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga aacaaacttg
    10261 gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg ggatcccctt
    10321 gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta gcctactctt
    10381 ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta ggatagactt
    10441 ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt ggctgggcat
    10501 tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta gctacatctg
    10561 ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag tgggcacatg
    10621 tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc cttgggcggg
    10681 tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg cgttggttgt
    10741 ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc atgacaacca
    10801 ggtcccaggt cccgaaaacc aaagaagaag aaccctaaca aagaggacaa gcggcctcgc
    10861 acagccttca ctgctgagca gctccagagg ctcaaggctg agtttcagac caacaggtac
    10921 ctgacagagc agcggcgcca gagtctggca caggagctcg gtacccggaa gatctggact
    10981 ctagagaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
    11041 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
    11101 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
    11161 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
    11221 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
    11281 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
    11341 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
    11401 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
    11461 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc
    11521 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaacca
    11581 tggaagatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    11641 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    11701 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg
    11761 caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg
    11821 tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct
    11881 atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc
    11941 tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg
    12001 gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca
    12061 gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg
    12121 cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat atgtggcgga
    12181 tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt
    12241 tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc
    12301 agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa
    12361 cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt
    12421 atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa
    12481 tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag
    12541 cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga
    12601 acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc
    12661 aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta
    12721 acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct
    12781 acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc
    12841 gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc
    12901 agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg
    12961 gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc
    13021 agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc
    13081 gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt
    13141 cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca
    13201 gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg
    13261 gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt
    13321 ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg
    13381 gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc
    13441 agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc
    13501 gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa
    13561 gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac
    13621 taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg
    13681 cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa
    13741 acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa
    13801 tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc
    13861 tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca
    13921 cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg
    13981 cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt
    14041 gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc
    14101 aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg
    14161 cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga
    14221 actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa
    14281 actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca
    14341 tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga
    14401 attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac
    14461 agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga
    14521 atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg
    14581 cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat
    14641 aataaccggg caggggggat ctaagctcta gataagtaat gatcataatc agccatatca
    14701 catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    14761 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    14821 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    14881 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccggaataac ttcgtatagc
    14941 atacattata cgaagttatg tttaaacggc gcgccccgga attcgccttc tgcaggagcg
    15001 tacagaaccc agggccctgg cacccgtgca gaccctggcc caccccacct gggcgctcag
    15061 tgcccaagag atgtccacac ctaggatgtc ccgcggtggg tggggggccc gagagacggg
    15121 caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag cgtgggcgcg
    15181 ggggccacgg cgcgcgcccc cagcccccgg gcccagcacc ccaaggcggc caacgccaaa
    15241 actctccctc ctcctcttcc tcaatctcgc tctcgctctt tttttttttc gcaaaaggag
    15301 gggagagggg gtaaaaaaat gctgcactgt gcggcgaagc cggtgagtga gcggcgcggg
    15361 gccaatcagc gtgcgccgtt ccgaaagttg ccttttatgg ctcgagcggc cgcggcggcg
    15421 ccctataaaa cccagcggcg cgacgcgcca ccaccgccga gaccgcgtcc gccccgcgag
    15481 cacagagcct cgcctttgcc gatcctctag agtcgagatc cgccgccacc atgattgaac
    15541 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    15601 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    15661 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
    15721 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    15781 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    15841 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    15901 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    15961 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    16021 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    16081 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    16141 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    16201 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    16261 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    16321 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
    16381 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    16441 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccacccccc
    16501 ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt
    16561 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    16621 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    16681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    16741 caatgtatct tatcatgtct ggatccgggg gtaccgcgtc gagaagttcc tattccgaag
    16801 ttcctattct ctagaaagta taggaacttc gtcgagataa cttcgtatag catacattat
    16861 acgaagttat gtcgagatat ctagacccag ctttcttgta caaagtggtt gatatctcta
    16921 tagtcgcagt aggcggtgtt tggttaggag aggaagtaga aggtcctcgg agtctagagc
    16981 ttatgtcttc tccattctag acaactgtca tcatgcccca ctcataccca gctctttctg
    17041 ctgagcagaa gaaggagttg tcggatattg ctctacggat cgtgaccccc ggcaagggca
    17101 ttctggctgc agatgagtcc gtaggtaagt gggagcctgt ggtcagggtt gaggagaggt
    17161 tgccaaggaa ttctagccaa agaaccagcc tgtctcccct ttgcttgctt ctaggcagca
    17221 tggccaaaag gctgagccaa attggggtgg agaacactga ggagaatcgc cggctgtacc
    17281 gccaggtcct attcagtgct gatgaccgtg tgaaaaagtg cattgggggg gtcatcttct
    17341 tccatgagac actctaccag aaagatgaca atggtgtccc cttcgtccgc accatccagg
    17401 ataagggcat tctcgtaggc atcaaggtac acagtccctg gtggtgcctg ggggctaggt
    17461 gtggaaacca gtgtatataa gagggtgaca agccagacct ctatctgttt cccttgcagg
    17521 ttgacaaggg tgtagtgcct ctagctggga ccgacgggga aaccaccact caaggtacct
    17581 gttgggtggg tacaggggtg ggacatgttg atgctggcaa gatggaggca gggtggtaag
    17641 gctggcactg tgttttcagg gctggatggg ctcttggaac gctgtgctca gtataagaag
    17701 gacggtgctg attttgccaa atggcgctgt gtactaaaaa tcagtgatcg cacgccgtcg
    17761 gcactggcca tattggagaa tgccaacgtg ctggcccgct atgccagcat ctgccagcag
    17821 gtgtgtgctg gggggttggg tggttggatg agggccccgg ggccggtgga gatgtgcttt
    17881 catggctttc tcccatccta tcttctactt cttccagtta accctgctct tgctttcaga
    17941 atgggatcgt gcctattgtg gagcctgaga ttctgcctga cggagaccat gacctcaaac
    18001 gttgccagta tgttacagag aaggtaagac caaacatagc cgggagggac agcctagcag
    18061 gggtgcctgt accccatccc tgctaagatc caggcacact ttcccttaag gatctttact
    18121 gatacccaaa cgggcttcat agactaagtc tatgctgagc ccttggtttc ctgtacccac
    18181 ctacccctgg gtgaggagca cagtgcgcta ggcagtggtc ccagccctgt acaaacctcc
    18241 tctgctcccc aggtcctggc tgctgtatac aaggccctga gtgaccatca tgtatacctc
    18301 gaagggactc tgctcaagcc caatatggtg acccctggcc atgcctgtcc catcaagtat
    18361 agcccagaag agatcgccat ggcaactgtc actgccctgc gtcgtactgt gcccccagct
    18421 gtcccaggta ctaaactgct cctatccctt caggtctgac ttgagggttt tccatgacac
    18481 cggggtcctt taccctgggc aagacagaga atgatcagtc agtctgtctt ctccttctac
    18541 tccaggggtg actttcctgt ctgggggtca gagtgaagag gaggcttctc tcaacctcaa
    18601 tgccatcaac cgctgcccac ttccccggcc ctgggccctc accttctcct atgggcgtgc
    18661 cctgcaggca tctgcactca atgcctggag aggacaaagg gataatgctg gggctgctac
    18721 tgaggagttt atcaagcggg cagaggtagg gagctagagg tgatgacttg gggagggatt
    18781 ctgtggacca cagaggtccc aaaccgggca gcgtgcattc cacatccgtg gtctggctca
    18841 gtctgctcat tgaatcccat tccgttttgc agatgaacgg gcttgcagcc cagggcagat
    18901 atgaaggcag tggagatggc ggagcggcag cacagtccct ctacatcgcc aaccatgcct
    18961 actgaggcac gctgactcta cccacagccc ttggcctagc aatgcacatc ccattttgcc
    19021 tatagtcaca gccagggcca aatagctatg cagaacagag atatctttat gagatggcgc
    19081 aacgcaatta atgataactt cgtatagcat acattatacg aagttatggt ctgagctcgc
    19141 catcagttca agaacagaac atgtggacct ttttcctgtc tccaccagcc cttacagtta
    19201 cccttgatgg tatagtttgt ccttgggctc tgcttgctaa ttttctctct tgggatcagg
    19261 gggacgtgaa ctccagccct gactcgtgct ccttatgctc tgagtacata gcaaataaat
    19321 gagagcaaaa cacagcacac cgctctgtct gtctgtctgt tgcacatacc aaagtctacc
    19381 tccatgtgtg cgacctcttg ctgatcctat ctcagagccc ctctgattgt ggtggtggtg
    19441 gtgaggtgag atctgagttt tcctgagctt caagttttgt ggaggaaggg ttcacctggc
    19501 tcaccatccc tagctcaggc tcctacctgc tgatttccaa agctggagtt gttgttagag
    19561 ggcctggggg tggagaggag ggtacaggtc ttccagggac tgacacatta aaggatccag
    19621 caggatgaga aactccattt tgtgcaaggc tcaaagggga caagcaggga tcagagtggg
    19681 ccagttctcc tccatctctg aagaatgtgc acttaggtct tttccaataa ggaaaccttc
    19741 caagcctcgg ggctacagag ctgagtgtct agaagcttgc caagggctat gacccacttc
    19801 catgtagaga agggattcat cggagggttc tggacttgtt agaactgata gggctttgtg
    19861 gtgaatgatt acccctgggc tagagtgtga ggtagggtga cactagacag atgtgcactg
    19921 gaaaagtcat tgatgttctg ctctgtgtgg cctgacctta taatcaaagc agctaactca
    19981 gcaatttcca tatgcttctg ccaggcactg ttcttctaac cattattata tgcattaaca
    20041 cagctctgta aagtaggtac cattgtactt ttcataggag gaaacaggct tcaaagagag
    20101 gctgagaggc tatttaggat tcgaacagca ggcattacta tatagaggct gtgctcagga
    20161 atttttcaat gaagcatcct caatcgctcg aattggaatc gatttttaaa tcctagtcta
    20221 tagtctcggc tagttgggag gctgaggtat cagacattag ggacaggatc actttaaccc
    20281 tctggatcgt atagggagat ctgtctcaac attaactaaa ataaaattaa tcacatagta
    20341 gccaaccatg ctaaaaagtt accgttttgt cagttttatc tttaagcttt gaagagctgg
    20401 tgctctgaag aaaagaccta tgttagaaat gacattaggg gctggtgaga tggcgcaggg
    20461 ggtaagagca cccgactgct cttccaaagg tccgaagttc aaatcccagc aaccacatgg
    20521 tggctcacaa ccacctgtaa cagagatctg atgccctctt ctggtacgtc tgaagacagc
    20581 tacagtgtac tcacatataa taaataaata aatctttaaa agaagaaaaa aaaaaaaaag
    20641 aaatgacatt agaaaacttt tcgggagtca ggttcttgat aagctcgact tcatttaagt
    20701 aaatattccc cttccgctga acgtggtggt gcatgtaatc ccctgcattg gagagggccc
    20761 agagtccgag gccagcctgg gctacatgga gattatcgcc ttctctaaac tggcgttgta
    20821 gagctaaaca gtaccaggtg cactcaatga aaagccaggg tcagattgaa agtgtagccg
    20881 agcgcgtacc ctctcacggg ctccgcctgc gcactggcgt gcgtcagcag tgttgctcgt
    20941 gattggcagc ggtcgcgcac cgtggtcccg ccccctcatt ctggagtttc cgattggaga
    21001 aagctcatga ggccccgccc catcggcgac ctgggcagcc ggaagggacc atggcggccg
    21061 caggagctgc ggccacggac ctaggtgcgg tggatttcag gtggagggcg cagtggggag
    21121 gtctcgcggg cagaatgtgt gggtggggcg cgagaagcct gacagctccc tccagcctgg
    21181 ggttgggggt ccgccacgcg ggagcggctt gctcggggtt cccggccgcc cgcactcctc
    21241 cacgatcccg gtgtggcggg catcgcaccc tagttcccgc gtcttagtcg gccctctgtg
    21301 ccctcagagg tggtccgggg caagcgttcc gcgcttttct tcgccgcggt ggccatcctt
    21361 ctgggactgc cgctctggtg gaagacgacg gagacttacc gggccccatt gccttactca
    21421 gacatcagtg ggctgaatgc tctgctggtg agactgggtg gggagaaccg gggctggggg
    21481 accggggggc agcccagctg taaggtgcag acattgctaa agaccccgat gcccctcatc
    21541 cctcgcgcag ctccggctca tggtgccagt cacagttgtg tttactcggg actctgtgcc
    21601 cctggacgac caagagaagc tacctttcac cgttgtgcat gagcgagaga tccctctgaa
    21661 atgtgagttc ccatgaagag gtgtcagggc tgctttttcc atctgagcca ggcccaaaac
    21721 ccagagttgg aatgcatagg aggggcgagg gcgactcata acttacttta aaatgtttta
    21781 ttacatttac ttagtgtgcg cgcgcacatg tatgtttgtg tggatgtctg tgctgtggag
    21841 catcttgtgg aggtcagagg atcacttgcg aaagtcattt ctcctcccac catatgggtc
    21901 ctggtcttgg ggatccaact caggtcatca ggcttggtat caagagccat ccgcctgctt
    21961 cccccaaatc ctaagaaaag agatctcctt tggaatgttc acttgggggt ttgtgttagt
    22021 ctaaagtaca gtgcctgtcc ttatgacagt gatttatgtc atacggtaaa ttatgccacc
    22081 ttatccttag caattgatac ccatcagtgc atagggaaag caggttgtag ctgaaagaaa
    22141 acgggagttt gaagttacat cggatgtgta ttcagacctt tgtcatttta acatcacaca
    22201 ctgtattgtt aggcactttg ttaggtacca agaacttgag ggtgaataat aaattggtaa
    22261 tattttaaag atgtttatag ttaacatcag aaagaaagaa actagcaaaa tactgtaagg
    22321 caggaggttt ggtctcgtgg tggagtcacg ggagagcctg aactccattc tcacctgccg
    22381 gggagtagaa aaaggtgcag aagggcttgg gcaatgtgat ttttgaggtt gggtgtttct
    22441 ggctttttga gacagggttt ctttgggtag ccctggctgt cctgggtctt gctgtgtaga
    22501 ccagacttgc accacacctg gcaaggactc tgatctttat tggatgcata atctcatgtt
    22561 cagttttgtt ttttcatcag tatttttttt ttttttttac taaatgattt aggttttatt
    22621 tcggtttcat gtgttctttt ttttttttta ttttaaatta cgtattttct tcaattacat
    22681 ttccaatgct atcccaaaag tacccccccc ctactcccct acccgcccat tcccattttt
    22741 tggccctggc attcccctgt actggggcat ataacgtttg cctgaccaat gggcctctct
    22801 ttccagtgat ggccgactag gtcatctttt gatacatatg cagctagagt caagagctct
    22861 ggggtactgg ttagttcata atgttgttcc tccgataggg ttgcagatct ctttagctcc
    22921 ttggatactt tctctagctc ctccattggg ggccctgtga tccatccaat agttgactgt
    22981 gagcatccac ttctgtgttt gctaggcccc ggcctagtct cacaagggac agctatatca
    23041 ccgtccttgc aacaaaggct tgctagtgta tgcaattttt tttttttttt tttgatgtta
    23101 gaatgtgatc tttggttcta gaattaaaat aacaatttga gagtcatcaa ggacctttta
    23161 taattccctc gcttgcttct cagcagcagc tgctaacagg acacagcttc cccggcactc
    23221 cttcaagcac cctttgtttt ttgaagcagc agcccctggt gtttagaacg attcctttct
    23281 agctcactct ttaaatcact ttagccacct gtctggt
//