LOCUS       KO-first_condition_ready_23954_MGI:101842        21454 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Pla2g1b targeting project(s): 23954
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 39386
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4432..4486
                     /db_xref="ENSEMBL:ENSMUSE00000189769"
                     /rank="1"
                     /note="ENSMUSE00000189769"
     misc_feature    4275..8507
                     /note="5 arm"
     misc_feature    8535..8559
                     /note="B1 Gateway"
     misc_feature    8572..8619
                     /note="Frt"
     intron          8620..9494
                     /note="mouse En2 intron"
     misc_feature    9492..9494
                     /note="Splice Acceptor"
     exon            9495..9667
                     /note="mouse En2 exon"
     misc_feature    9679..10272
                     /note="ECMV IRES"
     gene            10273..13333
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13372..13615
                     /note="SV40 polyadenylation site"
     misc_feature    13619..13652
                     /note="loxP"
     promoter        13679..14198
                     /note="human beta actin promoter"
     gene            14224..15018
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15218..15459
                     /note="SV40 polyadenylation site"
     misc_feature    15476..15523
                     /note="Frt"
     misc_feature    15530..15563
                     /note="loxP"
     misc_feature    9509..9586
                     /note="FCHK std_gpr"
     misc_feature    15579..15599
                     /note="B2 Gateway"
     misc_feature    8508..15629
                     /note="Synthetic Cassette"
     exon            16047..16206
                     /db_xref="ENSEMBL:ENSMUSE00001281065"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00001281065"
     misc_feature    15630..16362
                     /note="Critical Region"
     misc_feature    16386..16419
                     /note="Downstream LoxP"
     misc_feature    16363..16442
                     /note="synthetic loxP region"
     exon            17206..17333
                     /db_xref="ENSEMBL:ENSMUSE00000189767"
                     /rank="3"
                     /note="ENSMUSE00000189767"
     exon            19722..19935
                     /db_xref="ENSEMBL:ENSMUSE00000189771"
                     /rank="4"
                     /note="ENSMUSE00000189771"
     misc_feature    16443..21454
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaggcgca aatctgtggc attattttct cttacaggca aatccctctg
     4321 tgttgttccc ccgatagacc aaggccagcc cagagtggct aagagcaaag caaacagcct
     4381 tatcttggga gcgtggggcc ccatgtataa aagagggaag tcactggagt tctcccctca
     4441 ctccttctga agatgaaact ccttctgctg gctgctctgc tcacaggtag gcccctctgt
     4501 cgcctctctc tgagcacctt acggtctccc tcttctccaa tgcctccctc ccactggtca
     4561 cgctggaatt gtttggcatt ctccttaggg tctaatagcc tctgggttgg ggtctcctct
     4621 tcaccccagt gttcaggaca gctggtgctt ttcgacctct gggtctgaac tcagatgacc
     4681 ccttagaaaa tgctccctca tttgcctacc caagcaaagg aagccccctt ctgcctctga
     4741 tgtcttcttg ccctggcacc actttccttg gccttgccag tgccctaaca acttttcctt
     4801 ttctttttct ttaaaaaaaa atttttttcc ccaagtagta ctaatgtctt taatcccagc
     4861 tctcagaagg cagaggccag cagatctctg taagttcgag gccagtcttg tctacaaagc
     4921 aagtttcagg acagtcagag ctacacagag aaaccctctc tctctatata tagatataga
     4981 tagggtttct ttgtatagtc ctggctgtcc tgatatatat atatatatat atcattatgt
     5041 gtctgagcgt tttgcctgca catatggtat atattgtgtg agcagtgcct gcagagacca
     5101 gaagaagggt cagatcctgt agcactggag ttgtaaataa ctatgagctg cctgccatat
     5161 gggtgctaga accagacctg aatcccatgg aaggaacgaa agtgttcatg acttctgagc
     5221 catctctcca accgaccccc ttatcatcat caccatcaat agtaatcatt attattttat
     5281 tgatatgacc ttgctatatg gctcaagatg tctcctctcc acagtatagc ccagccttgg
     5341 gaccctcctc tctcagcctc tgaagtgtta ggattccatc tgtgcctggc ttgagtctgc
     5401 aatttttgtt ttattttgtt ttggttttcg agatagggtt tctttgtata gtcctggctg
     5461 tcctggaact cactttgtag accaggctgg cctcgaactc agaaatccgc ctgcctctgc
     5521 ctcccaagtg ctgggactaa aggtgtgcac cgccaccacg cccagctgat tcatttacat
     5581 attcaggcac catctgcata caggcattac atccccagct ccgtgaacag aaacagattt
     5641 gggccctgtg cacggagtca cctgtagttg gcacgtaagt cgtttctatt cacccagcaa
     5701 tgattgagac ctgcaatcag gctgtagact gagcctcaga gataaaagcg tcgtgtgcct
     5761 gttccaaaag cacatgtgtt tgcgcacacg cgcacactct gtgcgttagt tatgactgtg
     5821 tctagcttct gtaaagcata tgtctagctg tgtcacctag ggatacagcc cctacttact
     5881 gccaccacag gggtcaccca catcgtggga aaagccagct gctccctttc tttgtccctg
     5941 gggtctcccc acaaatggtt agccagaatg agcccaaaaa tagagtaagc agggcagggt
     6001 cttacgcgcc cttgatctcc acgctcagga ggctgaggca ggaggatttc tagtttgatg
     6061 tcagccttgt ttacatagga agaccccatt gtggtgctca cactcatcat tctagctctc
     6121 cagaggctga ggcagaagga ttgtcatgaa ttcaaggcca gcctattttt acaatgttag
     6181 ctcctgtctc agaaagaatg aataaaggag gccaagcaag gtgctgctgc gtgccttcaa
     6241 tcccagcact agggaaacag aggcaggaaa ctgtgaattc aaaaccagcc tggtctacag
     6301 agtgagttca tgccagccag gacagtatag tgagtgagac cttgtctcaa aaagataaaa
     6361 aagagaatgg aagaaagatg aaaagatgga actggacaac gtgtgagacg ataaggcagc
     6421 atggtgactg aggagatggg accccgaaag tcccttgttc tctgtgctct ttatgcagca
     6481 ggactctgtg caagcggctg acggcctctg ctccacgagc tgctctgcaa agcctgtgct
     6541 ttgggcaagc gcagtcatgg cacaggtttg ctcattcatt caacagaatt ttgtggagtg
     6601 tctactaggt accaggcaca tacttcactc actgacttta ttcatggctt atctagagtc
     6661 acatgcacac acacacaaac acaaatacac tcactgcata gacacaaaca cagatatacc
     6721 acacacacaa aacacacacc acacacaaat atgcacaaac aacacacaac acaatacaca
     6781 cacatataca cacaaagaga cacacacacc acacacgcaa gcagatatac accacacata
     6841 tacagacata cacaaacaca cacaaacaac acataacaca atacacacac atacaccaca
     6901 cacacaggca cacacatacc atacacaaat acatatcaca cacacataca ccacaacgtg
     6961 tgttaagcac agtgataggc tttaaggaga atgaaagcca ggagacctgc tcttgagaac
     7021 ctcctgtcct accgactgag tgggaacaca ttagctagga tgccttagca cccacagaaa
     7081 aatccaggca cagcagcgac acccctacaa ttccatgact tcagagatgg agacaggtgg
     7141 aagctctccg gggcttgctg cttagccacc atagccaagc ccccagctct cagccaccat
     7201 agccaagtgg caagccccgg gctcactgag agactcggtg tcaagaataa gagagtgagt
     7261 taagattcca gatgttgacc tctgccctcc acctacatat gcacacatac acacacatat
     7321 gtcagtccaa acacaaaccg aagtactatg aagcaaaaaa acatgggaca gtagagacac
     7381 tgcttggatg tgtggaccgg aagatcctct ttaatgggga agcatcgagg ccaagatctc
     7441 tctagccatt caggaggctg aagcaggagg attgagaatt taagacttgg actatagggg
     7501 ctggtgagat ggctcagtgg gtaagagcac ccgactgctc ttccgaaggt ccagagttca
     7561 aatcccagca accacatggt ggctcacaac catctgtaat gagatctgac tccctcttct
     7621 ggagtgtctg aagacagcta cagtgtactt acatataata aataaatctt taataaaaaa
     7681 aaaaaaaaaa aaaaagactt ggactataaa ccgggcgtga tggagcacgc ctttaatccc
     7741 agctctcggg aggcagaggc aggtgaattt ctgagttcga ggccagcctg gtctacaaag
     7801 tgagttccag gacagccagg gctacacaga gaaaccctgt ctcaaaaaaa ccaaaaaaaa
     7861 aaaaaaaaaa aaaaaagact tggactatag gtagactcaa aaaaagaaag agggggagag
     7921 agagagagag agagagagag agagagagag agagagagag agagagagga ttaaataaat
     7981 agtaacattt cagtcagtcc aggaactctc agaaagtgac tcagagagtg gcaagcctat
     8041 ttgtgcttcc ggccacttgt ccattggtga cctgggcagg agtggcagtg gagagaaaga
     8101 agagatgaag tggagtccca gacccatgga agggagaagc ctcaggctga ggtgcagggc
     8161 cgacactggg gctgagggaa atccaggatg tttgtgcatt tatgaacaca gccctgaagg
     8221 gcacagcgag acaaactcag aactggtcca cactggcctg cgctgaccca gcttcggctc
     8281 agacctactt acttactgat tgccctcctg ttggctgttc ctgcgtgtgg gaggcactga
     8341 gcaccacacc attctttcat ccttgcctcc atcttcctcc tcctccaact tcttttcctg
     8401 aggcagggta acacgtagcc caggctcccc attcaacaac aacaaaaaga agttcaaatt
     8461 gaagttatct tcttacaagg caagtttaag gctttcccag gttatgtaag gcgcataacg
     8521 ataccacgat atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct
     8581 attccgaagt tcctattctc tagaaagtat aggaacttcg aaccctttcc cacaccaccc
     8641 tccacacttg ccccaaacac tgccaactat gtaggaggaa ggggttggga ctaacagaag
     8701 aacccgttgt ggggaagctg ttgggagggt cactttatgt tcttgcccaa ggtcagttgg
     8761 gtggcctgct tctgatgagg tggtcccaag gtctggggta gaaggtgaga gggacaggcc
     8821 accaaggtca gccccccccc cctatcccat aggagccagg tccctctcct ggacaggaag
     8881 actgaagggg agatgccaga gactcagtga agcctggggt accctattgg agtccttcaa
     8941 ggaaacaaac ttggcctcac caggcctcag ccttggctcc tcctgggaac tctactgccc
     9001 ttgggatccc cttgtagttg tgggttacat aggaaggggg acgggattcc ccttgactgg
     9061 ctagcctact cttttcttca gtcttctcca tctcctctca cctgtctctc gaccctttcc
     9121 ctaggataga cttggaaaaa gataagggga gaaaacaaat gcaaacgagg ccagaaagat
     9181 tttggctggg cattccttcc gctagctttt attgggatcc cctagtttgt gataggcctt
     9241 ttagctacat ctgccaatcc atctcatttt cacacacaca cacaccactt tccttctggt
     9301 cagtgggcac atgtccagcc tcaagtttat atcaccaccc ccaatgccca acacttgtat
     9361 ggccttgggc gggtcatccc cccccccacc cccagtatct gcaacctcaa gctagcttgg
     9421 gtgcgttggt tgtggataag tagctagact ccagcaacca gtaacctctg ccctttctcc
     9481 tccatgacaa ccaggtccca ggtcccgaaa accaaagaag aagaacccta acaaagagga
     9541 caagcggcct cgcacagcct tcactgctga gcagctccag aggctcaagg ctgagtttca
     9601 gaccaacagg tacctgacag agcagcggcg ccagagtctg gcacaggagc tcggtacccg
     9661 gaagatctgg actctagaga attccgcccc tctccctccc ccccccctaa cgttactggc
     9721 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     9781 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     9841 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     9901 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     9961 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
    10021 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
    10081 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
    10141 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
    10201 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag
    10261 cttgccacaa ccatggaaga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10321 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
    10441 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
    10501 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
    10561 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
    10621 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
    10681 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
    10741 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
    10801 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag
    10861 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
    10921 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
    10981 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
    11041 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
    11101 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
    11161 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
    11221 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    11281 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
    11341 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    11401 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
    11461 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
    11521 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
    11581 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    11641 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
    11701 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    11761 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    11821 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    11881 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
    11941 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    12001 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
    12061 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
    12121 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    12181 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
    12241 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
    12301 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
    12361 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
    12421 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
    12481 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
    12541 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
    12601 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    12661 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    12721 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
    12781 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
    12841 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
    12901 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
    12961 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    13021 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    13081 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
    13141 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    13201 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    13261 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
    13321 tgtcaaaaat aataataacc gggcaggggg gatctaagct ctagataagt aatgatcata
    13381 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    13441 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    13501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    13561 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggaat
    13621 aacttcgtat agcatacatt atacgaagtt atgtttaaac ggcgcgcccc ggaattcgcc
    13681 ttctgcagga gcgtacagaa cccagggccc tggcacccgt gcagaccctg gcccacccca
    13741 cctgggcgct cagtgcccaa gagatgtcca cacctaggat gtcccgcggt gggtgggggg
    13801 cccgagagac gggcaggccg ggggcaggcc tggccatgcg gggccgaacc gggcactgcc
    13861 cagcgtgggc gcgggggcca cggcgcgcgc ccccagcccc cgggcccagc accccaaggc
    13921 ggccaacgcc aaaactctcc ctcctcctct tcctcaatct cgctctcgct cttttttttt
    13981 ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac tgtgcggcga agccggtgag
    14041 tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag ttgcctttta tggctcgagc
    14101 ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg ccaccaccgc cgagaccgcg
    14161 tccgccccgc gagcacagag cctcgccttt gccgatcctc tagagtcgag atccgccgcc
    14221 accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
    14281 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
    14341 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
    14401 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
    14461 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
    14521 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
    14581 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
    14641 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
    14701 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
    14761 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
    14821 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
    14881 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
    14941 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    15001 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
    15061 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    15121 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    15181 tcgcccaccc cccggatcta agctctagat aagtaatgat cataatcagc catatcacat
    15241 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    15301 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    15361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    15421 tgtccaaact catcaatgta tcttatcatg tctggatccg ggggtaccgc gtcgagaagt
    15481 tcctattccg aagttcctat tctctagaaa gtataggaac ttcgtcgaga taacttcgta
    15541 tagcatacat tatacgaagt tatgtcgaga tatctagacc cagctttctt gtacaaagtg
    15601 gttgatatct ctatagtcgc agtaggcggg cacaaagtcc tggcttccat ctgcaaactt
    15661 cttatttttt ttaagatttg tttattatta ccaggcagtg gcacatgcct ttaatcccag
    15721 cacttgggag gcagaggcag gtggatttct gagttcaagc ccagcccggt ccagtggtgc
    15781 tcgtttatag tcctagcaca aggggaggat gaggcaggag ggttgctgct gagttggagg
    15841 ctagacacct tcctgctcag gttcgactta gctccaagat ctaaataatg aaaagagaca
    15901 atcatccgca cattagtagg cttctgggaa ccgatgctct cccgaggcag caactgatag
    15961 gaagcgagaa agaagataac aggcaggctt cggaccccgg gtaaagcagt tcggagtgga
    16021 tttgaatgtg ctgtacactc ttgcagcagg cgctgctgca cacagcatca gccctcgggc
    16081 tgtgtggcag ttccgcaata tgatcaagtg caccatcccc gggagtgatc ccctgaagga
    16141 ttacaacaac tatggctgct actgtggctt gggcggctgg ggcaccccag tggacgactt
    16201 agacaggtga gtggtctatc ccatgggaga ctggagcgtg ggcacggggg tggggccaga
    16261 gaaccggcca atcacatctc gtctcgagag gcaggaaggg gaggcatgca cagtccctga
    16321 gcgtcgtgtg tattcttttt gtttagttat tcattattta cagagatggc gcaacgcaat
    16381 taatgataac ttcgtatagc atacattata cgaagttatg gtctgagctc gccatcagtt
    16441 caagcatata ttttcacctc ttgttatgta aagaaagagg tcctagagga cccattaatc
    16501 tagtggtctc cgaaagtgga aagtggaaat gtaaagtgcg cgatgctacc gtctagtggc
    16561 agagcagggt actgccaacc atccacatgt cagccttggc tttaaaaaag tcatcgcaac
    16621 ttcataactt gtaatttcac tgtattcctg cagctttgaa aaaaaaaaaa aggaggagac
    16681 agaggcaggc ggatttctga gttcgaggcc agcctggtct acagaatgag ttctaggaca
    16741 gccagaacta tacagagaaa ccctgtcttg aaaacaaaaa acaaaaaaac aaaacaaaaa
    16801 agtcattttt tgagacaggg tcttgctgta tagccctggc tgatctcaaa ctcagagatc
    16861 cacctgcctc tgcctcaagt actgggattg tctttttttt ttttccagag ctgaggaccg
    16921 aacccacagc cttgttcttg ctaggcaagc actctaccac tgagctaaat gcccaaccct
    16981 caagtactgg gattgaataa aggcgggggc caccacacct ggaaaaagta gctttcaata
    17041 aatatatgct aatagcgatc acagaatctc gagtcattct ccagccattg cttctggaac
    17101 attggtgctt aaatcagcct cgcttgcttg agaaagactg caagctgaga accgacctcc
    17161 ggtgggtgca ctaccccacc ttcagccatt tcttcctctc tccaggtgct gccagactca
    17221 tgaccactgc tacagtcagg ccaagaagct ggaaagctgt aaattcctca tagacaaccc
    17281 ctacaccaac acttactcct actcatgctc cgggagcgag atcacctgca gcggtagggt
    17341 taccttctga gacctcggga ctctctacct ggcctctggc gggtttggaa gggggaggga
    17401 gggacaggga cagtcaccat ttagtgtgcc cacatttgac agattttatg tatttacggg
    17461 aggcatgtgt atggcattac acacgtgcac agctttcggg agtcagttct ttccttccac
    17521 catgtgaggc ccgggaatgg gacccaggct cagacttagc accaagcccc tttgttccct
    17581 gagccacctt gcctccccaa agcttctccc cttaactgct atgttatata ctgattttgt
    17641 attttctaaa cacgtggaag ctgtatcatt gcttatatgc caaacactga actaaacatc
    17701 tattcgggaa agatttctca tccccacgtt acagacgagg aatcagaggg ttgggaatgt
    17761 aactcagtta tagatcgctt gtctagcatg cacagagccc tgagttccat ccctggaacc
    17821 tatacattga tatggtggtg catacctgta accccagcat gtagaggcag gacatataat
    17881 ttaaaaaaag atttatctat tatatatata catatgtata tatataatag ataaattttt
    17941 tggtatatat atatatatat atatatatat atatatatac cattgctctc ttcagacaca
    18001 ccagaagagg tcatcagaac ccattacaga tggttgtgag ccaccatgta gttgctggga
    18061 atttgaactc aggacgtctg gaatggcagt tagtgctcct aactgctgag ccctctctcc
    18121 agcccaggat atataattta taagtggtag ttgatgtaag aattaatatt acaaatgtat
    18181 tcaggaaatt ggtagagcag cagtttttct taaaccttgc ttgtccccaa ataatgacat
    18241 agagactaag atttatttgt gtaaaagcac aataactaga cagtataatt ctagtctaat
    18301 cctctgatct aaccttgcta cttcccagcc aaagtcccca taatccttgc acttagagcc
    18361 ttccacgctc aagttaaatt ctcaagcctg ttccttcctc cttcctcgtg cccaagtctc
    18421 cctgcctctc tgaatccttt ctcctcccac aagtcccgtc tctcctagct attggctttt
    18481 ccagatttta ttgacaagtc agagaataaa tggtgtgcat tgtttacaca aacttgagac
    18541 aggagatgta cataactagc tgtcttcaga cacaccagaa gagggcgtca gatttgatgt
    18601 atgagtatct gcacgtacac ctgctcgcca gaagagggcg tcagatctca ttacagatgg
    18661 ctgtgagcca ccatgtggct gctgagattt gaactcagga cctctggaag agcaatcagt
    18721 gctcttaacc gctgagccgt ctctcccgcc ccttgagaca ggagattctt aatataagca
    18781 ttgcaatacc ggctccagat taatggtggg atagagagat cagcatttgc atactacaag
    18841 ggtaatcttc acacaattca caaaaatatt attacagcca atatttgtac ttttctgatt
    18901 tttaggttta tctgcttatg tttttttttt gtttttgttg ttttgttttg tttttttgag
    18961 atagggtttt tctgtgtagc cctggctgtc ctggaacttg ctctgtaaac caggcaggcc
    19021 tcgaactcag ggatcctcct gcctctgcct cccaagtcct gggactaaag aggtgcacca
    19081 ccactcctga caatgtttct ttattttatg tctaagaata agttggcatc ctggtgtctt
    19141 ctatcactct ctgtcatatc tttagagacc cagcctctca ctaatcaagg cactcacctg
    19201 tagcctgaca ggccaacaag tgctggcgac ttgtgctctc cacttactga cagagttgca
    19261 gatgccccaa ccacaccttg ctcaagggct cggggatcct cagttagagg ctggagcttg
    19321 cgtgttatcc acttgatcca ctgagcccaa gaagtacctt tctttctttt ttttttaaga
    19381 tttatttatt ttatatatgt aagtacactg ttgctgtctt cagacacacc agaagagggc
    19441 gtcagattcc attatagatg gtcgtgagcc accacgtggt tgctgggatt tgaactcagg
    19501 acctctggaa gagcagtcag tgctcttaag cgctgagcct tctctccagc acaagaagta
    19561 catttctatt ataatttggc aaccccaacg tactttcgtt ttgcttctgt ctctcaaccc
    19621 tacatctgca acccacggaa tggtcaccag gttctttcca ttctgtcatg tggcgtcctg
    19681 ggctccactc gattcgattc tctgttcttg tttctctcta gccaaaaaca acaaatgcga
    19741 ggacttcatc tgcaactgtg accgtgaggc cgccatctgc ttctccaagg tcccgtacaa
    19801 caaggaatac aaaaaccttg acaccgggaa attctgttag cctgtcacct cacttcctgc
    19861 ccacgccgac cccgcccacc ttgctgtctt atttcaccct gcgccctcta ataaagtacc
    19921 tgctgtcaga gcctggtttt ggtgattgtt atctctgtcc accgagcagg gctagatcct
    19981 cagaggaaag cattccgtct agccttggaa acgcaagatg aaagagaaca tttccaggaa
    20041 tcaagcgcct cctagaatgg acgataatcc gggtgatggt agtgcacacc tttcatcccg
    20101 gcacacagga ggcagaggta ggcaggtttc tgtgagtttg aggccagcct ggtctacagg
    20161 gtgagtttca ggaataacca actgtcttgg aacaaaagaa taagaaaagt ggctgctggt
    20221 ttgcaatcac gatcaatgga ttaccctgca cacagcctgt gcctctctaa ggtctcgatc
    20281 gtctgctgta tggcgactat gaggaatctt gtttttctaa gctgagaccc ggtccgatag
    20341 taggatggga agccggctct gatttgtttg aaccagtttt catctgagct catccttctg
    20401 cttctatttg caataatctc aacccagtct ttttatttgt ttgtttttgt ttttccttct
    20461 ttgagatagc ccggttggcc tggaactcat tatgtatgat aattttttga gacagggttt
    20521 taccgtgtat tcctgggact cactatgtaa cccagtcact cgtttccagg tgacaggcta
    20581 agcttagaac ctcaatcttc ggaagtctcc cgagcttgtg aaccctaagg aggcagagtg
    20641 aggcagctta gccacctttg aatctctggc tcccagggtt ctttggaaac ccctgctgag
    20701 cgtcgaggct cagagtagca accctaatcc tcaaagaact gtgaaggccc tgcggaaaga
    20761 agctggaagc ttagtgtatg gcagaaaaat aggcagtcag gcgtttgttc ccatagttct
    20821 ttcacgtgat tgaaacaaaa gagaacggag aacacagctc aggtgacagt gtgctggcct
    20881 aggatgcagg aggccctggg ttcgactccc aatagctaat aaacctgggc atgggcgcat
    20941 gcctgtaatg tacccagcac ccagaaggtg ggggcaggag gatcaggagc ttcgggtccc
    21001 cttagctaca gagtgaatta gaagccagcc tgacatacaa aacaccagat cgtctagacc
    21061 tgcgcacagc ctggttaatc tctgtccctg tctcccctgt cccgtgcgca caacctggtt
    21121 aatccctggt tccccagtca acggatttcg gagtcaggtg tctgattggc tgctgtgagg
    21181 acacgtggtt cttgattgcc cctccccttc acctccctcc cccccaacct cctctgtctc
    21241 tctcgcgcgc tctctctctc tctctctctc tctctctctc tctcacttta gttatccacc
    21301 cctcccttcc caaaggtcag gccaggccac ctgcgcaggc actgtgatcc gtgaccccgg
    21361 tagtagctcg taattgctgt ccagaatcca gctggaaggg ggtagaagtt tgaacatgaa
    21421 accccacatg gtttctatcc tggtaagggt gaga
//