LOCUS       KO-first_condition_ready_32420:120602_MGI:101813        19988 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Coq3 targeting project(s): 32420:120602
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 88294
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6600..6752
                     /db_xref="ENSEMBL:ENSMUSE00000177575"
                     /rank="3"
                     /note="ENSMUSE00000177575"
     misc_feature    4275..8553
                     /note="5 arm"
     misc_feature    8581..8605
                     /note="B1 Gateway"
     misc_feature    8618..8665
                     /note="Frt"
     intron          8666..9540
                     /note="mouse En2 intron"
     misc_feature    9538..9540
                     /note="Splice Acceptor"
     exon            9541..9713
                     /note="mouse En2 exon"
     misc_feature    9725..10318
                     /note="ECMV IRES"
     gene            10319..13379
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13418..13661
                     /note="SV40 polyadenylation site"
     misc_feature    13665..13698
                     /note="loxP"
     promoter        13725..14244
                     /note="human beta actin promoter"
     gene            14270..15064
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15264..15505
                     /note="SV40 polyadenylation site"
     misc_feature    15522..15569
                     /note="Frt"
     misc_feature    15576..15609
                     /note="loxP"
     misc_feature    9555..9632
                     /note="FCHK std_gpr"
     misc_feature    15625..15645
                     /note="B2 Gateway"
     misc_feature    8554..15675
                     /note="Synthetic Cassette"
     exon            16087..16186
                     /db_xref="ENSEMBL:ENSMUSE00000177581"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000177581"
     misc_feature    15676..16335
                     /note="Critical Region"
     misc_feature    16359..16392
                     /note="Downstream LoxP"
     misc_feature    16336..16415
                     /note="synthetic loxP region"
     exon            17277..17519
                     /db_xref="ENSEMBL:ENSMUSE00000177577"
                     /rank="5"
                     /note="ENSMUSE00000177577"
     misc_feature    16416..19988
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattgcca aggatttcta tttgtcattc cacccatatt cctaaaaaaa
     4321 attaggaaag tgatacttga atttaaattt tttaaaatta caattgtttg tttgcttgtt
     4381 tggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtagggaggt
     4441 gagtcatgga taccaaaccc ctttgtggag gtcagagaat aacttgcagg catctgttgt
     4501 ctccttcaac catatgggtc ccaaggatca aaagcaggtt gtcagacttg gtagcaagta
     4561 cctacacact gaaccatttc actggcacat gatacatgaa tttaaattaa aaaaaaagag
     4621 agactaatga ctgcagaatg tgctctctaa atgtattcaa gagaaacagc aaacatttga
     4681 gtggtattat tttttatgac ttaacagtct aattatcttc tgctgagcag aactttggag
     4741 ttgttctgca ttgcactaac atgtgcttca gtgttgtgcc tagttttgtt ttgcttcccc
     4801 ttctcatggg attaaaaaac tgaaaacaaa ttatatgttt ttcttatccc ttttcttcaa
     4861 attatctatt tgacaattat ctcctgaaga acgatgctct aggaactgtt ctagtcactg
     4921 gaaataaatg atattcaaga tcagtccctg ccttcccttt atagtaggta tgctagggag
     4981 taacagacgt cagatgactg acagacagat gactgtacag cccagggata agcacagagt
     5041 gatgcaggag ataaaacagg agaatgtgac caacatttga gcaccaagga agagttttgt
     5101 gataacattt tatttaaaat aagacttgag ggatgagaga cttaggtgct tagtagagga
     5161 aagaaagggc tgtagggaaa atggtcttga agaagaaaca atatacacaa ggtgcgggaa
     5221 gaaagagtga ttgtagccaa ttcacaggca atatgactaa tgggggaaga tcaggaggaa
     5281 atgagtttga gaaagaagga agctgggtgc tccagtcctt cttagaaggg ggaacgaaat
     5341 actcacagaa ggaaatatgg agacaaagtg tggatcagag actgaaggaa aggccattca
     5401 gagactgccc cacctaggga tccatacagt tgccaaaccc agacactatt gctgatgcca
     5461 ggaagtgctt gctgaaagga gcctgatata gctgactcct gagaggctct gccagtgcct
     5521 gacaaataca caggcagatg ctcgctgcca accattgaac tgagcacttg gtccccgatg
     5581 gaggggttgg agaagggact gaaggagctg aaggggtttg cagccccata ggggaagcaa
     5641 cagtgtcaac ggaccactgg agctcctggg gattggacca ccaaccaaag agttcacatg
     5701 gcagaaaggg gccttgttgg ttatcagtag gaggagtggc ccttgggcct gagagggttt
     5761 tatgccccag tgtaagggaa tgccagggtg ggaaggcggg ggtagggggg tgggggggtt
     5821 ggggggagct ccctcataga ggtaggggga ggggggtggg atagggagtt tccagaaggg
     5881 agacctggaa aggggataac atttgaaatg taagtaaaaa aaatatccaa taaaagaaag
     5941 acaaaaaagg aaggaagctg gggactccat aattctgagc tttctgtcat tccaaggatt
     6001 ttagtctttt ctctaatgac actggaatcc ttgaaagcat tttatgtgag aaacaaatta
     6061 actagatttt tttttacttc agaataacgt ctcttttgtt tatcatggaa atcacattag
     6121 aaggtggtga tgtatggagc agcagactcg ttagaagagt aagaccgtag tcagggaggg
     6181 gatggtagag tttgatgaga gccgtggaaa tggaggcaga gggagaatgg taggctctct
     6241 ggtgcttaac cactttcccg agttctctga acttttctca gccacaaaat gaagtaatag
     6301 tattatagtc taaaaataac gtgtttctta gctagtaccc agcatttagt aagtattcaa
     6361 caagtgatag ctcttgttgt tgttattatt gttattgtct taagtgtgac ccagaacagc
     6421 aggcttggtc ctcaaaagtt tgtgaacata tatgggcatg tgatggtaat cttaaagtat
     6481 caaaattgag ccatctatat gtttagaatc taataaatta gaatctttct atgtaatata
     6541 ttctgggaat gcatttttag aacaatgata atgcttggtt ctttgttttc atcccttagg
     6601 accccttgga agagattgta cagtacttca caaacaacag ttgacagcag ggaggtcaaa
     6661 aacttccagg ccctggctca cacgtggtgg gatgagtacg gaaagtttgc gcctcttcat
     6721 tctatgaatg acctacgggt gccattcatt aggtaacagt ccaggaactc cctttaagtc
     6781 ttaaaagtac atccttcgct tatttgcatt cacttccctt ttgctttatt tacattcaaa
     6841 attttacatt ttagggttgc tttggaaatt agaaatattt ctcatcatga gttaaaagta
     6901 ttagctttag ggtggagagg tggttcagta gttaagacct cttgctgctc aaatagggtt
     6961 tggttcccat cacccacatc aggcagctta caaacactcc agctccacgg ggtctgacat
     7021 cctcacttgg cttccaaggg caccctggaa gcataattta aatctaaagc ataatttaaa
     7081 attaaaaaat taaaattaaa aaaaatcttt aaagccatta acttacagaa acaatactaa
     7141 aatcccacgt tgcttataca atgtaactga aaacattttt gaccctatta ggtcagttag
     7201 aattcagtcc tgatgaaatg gaactagcat gatgtgtctt gaaggtcgtt ctatagttgc
     7261 tgacctgcgg tggccttggt gacctttagg ggattctgat aaaggagtca ttgcaaagac
     7321 agattggctc accagactct gagagcttac attgatgggt ctctagtacc agcctttgca
     7381 acccacttgg ataagcgaaa tgggaaccat gtgtttcaat gggaggtaaa gaaaggaagg
     7441 agttgagagg gcttgccagt gatgcagctt gcatttcagc aagtcttctg ggagattcca
     7501 gcattactac gtgtgagaac tgtggtcaaa caccagtagc atctttattt gtttacttac
     7561 ttatttactt tctatgtgca atggtgtttt tcctgcatgt gtgtttgtat gagggtgtta
     7621 gaatctggag ttacagacag ttgtgtgttg ctttgtgggt gctgggaatt gaacccaggt
     7681 cctctggcaa agtagtcagt gctcttaacc actaagctat ctctccagct cttatttatt
     7741 tatatatcac ataaaacaga tcagctgggc gtggtggcgc acacctttaa tcccagctct
     7801 tgggaggcag aggcaggtgg atttctgagt tcgaggccag cctggtctac agagtgagtt
     7861 ccaggacagc aagggctata cagagaaacc ctgtctcgaa aaaccaaaaa aaaaaaaaaa
     7921 aaaaccaaaa aaccaaaaaa caaaaaacag atcatctagt atgatgcaaa ctgtgagtac
     7981 atgcttggat tttgcttttg ttataactat aagccatcag aagaaaaaaa atatgatttg
     8041 aaaatagtag catccttttg tgactgtttg aagaatggat tggacttcat tggaaacaga
     8101 tcatttccta tgaaatcctg aagtgatcta cttagctaat gtctgtatta ttttaagaga
     8161 ctaagatgtt cattttatat atatgtttat tttagtagat gtataattaa atttgtgtcc
     8221 aatttaaagg ttgatttata attccatacc atgaattatg taacagatta tgtaggtgcc
     8281 aatacgtttg tgtgtgtatt aaagcattac tgtgattatt gcagaatacg tttatttgac
     8341 tttggtataa gtgtacatag tcatacatga gaaagccaac agtttagaag attaaataca
     8401 tgtggaactc ttatctgtaa ataatgcatg aacttatctt agaaacttct gaaaacatag
     8461 tcaacagagg ctttaaattt tcatcacttg tatttaaaca gtttgactga aaaggtattt
     8521 gagtaaagtt catttgacta ctaggctgaa tttaaggcgc ataacgatac cacgatatca
     8581 acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa gttcctattc cgaagttcct
     8641 attctctaga aagtatagga acttcgaacc ctttcccaca ccaccctcca cacttgcccc
     8701 aaacactgcc aactatgtag gaggaagggg ttgggactaa cagaagaacc cgttgtgggg
     8761 aagctgttgg gagggtcact ttatgttctt gcccaaggtc agttgggtgg cctgcttctg
     8821 atgaggtggt cccaaggtct ggggtagaag gtgagaggga caggccacca aggtcagccc
     8881 ccccccccta tcccatagga gccaggtccc tctcctggac aggaagactg aaggggagat
     8941 gccagagact cagtgaagcc tggggtaccc tattggagtc cttcaaggaa acaaacttgg
     9001 cctcaccagg cctcagcctt ggctcctcct gggaactcta ctgcccttgg gatccccttg
     9061 tagttgtggg ttacatagga agggggacgg gattcccctt gactggctag cctactcttt
     9121 tcttcagtct tctccatctc ctctcacctg tctctcgacc ctttccctag gatagacttg
     9181 gaaaaagata aggggagaaa acaaatgcaa acgaggccag aaagattttg gctgggcatt
     9241 ccttccgcta gcttttattg ggatccccta gtttgtgata ggccttttag ctacatctgc
     9301 caatccatct cattttcaca cacacacaca ccactttcct tctggtcagt gggcacatgt
     9361 ccagcctcaa gtttatatca ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt
     9421 catccccccc cccaccccca gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg
     9481 gataagtagc tagactccag caaccagtaa cctctgccct ttctcctcca tgacaaccag
     9541 gtcccaggtc ccgaaaacca aagaagaaga accctaacaa agaggacaag cggcctcgca
     9601 cagccttcac tgctgagcag ctccagaggc tcaaggctga gtttcagacc aacaggtacc
     9661 tgacagagca gcggcgccag agtctggcac aggagctcgg tacccggaag atctggactc
     9721 tagagaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     9781 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     9841 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     9901 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     9961 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
    10021 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
    10081 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
    10141 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
    10201 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
    10261 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccat
    10321 ggaagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
    10381 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
    10441 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
    10501 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
    10561 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
    10621 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
    10681 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
    10741 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
    10801 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
    10861 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
    10921 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
    10981 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
    11041 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
    11101 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
    11161 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
    11221 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
    11281 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
    11341 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
    11401 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
    11461 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
    11521 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
    11581 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
    11641 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
    11701 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
    11761 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
    11821 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
    11881 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
    11941 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
    12001 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
    12061 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
    12121 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca
    12181 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
    12241 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag
    12301 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact
    12361 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc
    12421 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa
    12481 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
    12541 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
    12601 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
    12661 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
    12721 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
    12781 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca
    12841 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
    12901 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa
    12961 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa
    13021 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
    13081 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
    13141 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
    13201 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
    13261 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc
    13321 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata
    13381 ataaccgggc aggggggatc taagctctag ataagtaatg atcataatca gccatatcac
    13441 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    13501 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    13561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13621 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggaataact tcgtatagca
    13681 tacattatac gaagttatgt ttaaacggcg cgccccggaa ttcgccttct gcaggagcgt
    13741 acagaaccca gggccctggc acccgtgcag accctggccc accccacctg ggcgctcagt
    13801 gcccaagaga tgtccacacc taggatgtcc cgcggtgggt ggggggcccg agagacgggc
    13861 aggccggggg caggcctggc catgcggggc cgaaccgggc actgcccagc gtgggcgcgg
    13921 gggccacggc gcgcgccccc agcccccggg cccagcaccc caaggcggcc aacgccaaaa
    13981 ctctccctcc tcctcttcct caatctcgct ctcgctcttt ttttttttcg caaaaggagg
    14041 ggagaggggg taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg
    14101 ccaatcagcg tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc
    14161 cctataaaac ccagcggcgc gacgcgccac caccgccgag accgcgtccg ccccgcgagc
    14221 acagagcctc gcctttgccg atcctctaga gtcgagatcc gccgccacca tgattgaaca
    14281 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    14341 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    14401 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
    14461 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    14521 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    14581 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    14641 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    14701 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    14761 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
    14821 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    14881 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    14941 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    15001 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    15061 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    15121 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    15181 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg
    15241 gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta
    15301 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    15361 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    15421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    15481 aatgtatctt atcatgtctg gatccggggg taccgcgtcg agaagttcct attccgaagt
    15541 tcctattctc tagaaagtat aggaacttcg tcgagataac ttcgtatagc atacattata
    15601 cgaagttatg tcgagatatc tagacccagc tttcttgtac aaagtggttg atatctctat
    15661 agtcgcagta ggcggctggg attaaaggag tgtgccacca cacccggcta atttttttct
    15721 ttagatgagt attagagcat taatgttgga ttatctcaga actttgatta ttgaaaatgt
    15781 acaaattaag cagatcattt cattctgaaa aataaaagat acgcttctaa aaccttcagt
    15841 gattctattc aaatgaggac tgtgaactag gcatggtggc agcactcctg tgattcccga
    15901 actccggtgt ctgagcaagg aagattggga gctcaaagta cgtctaagcc ttatagcgag
    15961 cctctggctc aattaagaca gtgaaataaa caagttaaat taaggggaat atttccttga
    16021 agactgtata tattgttgac aaatgtagct gtctgttttt aaatattttg aattttgttt
    16081 ttaaagagac aatcttttga aaacaagtgc tagtcaccat ccaggaaagc ctttatctgg
    16141 gatgaagatt cttgacgttg gctgtggcgg tgggttatta actgaagtaa gtgcctcaga
    16201 gagagagccg tgtccacttt tcatgcacat gagaattttc taatgtacct cataatgcta
    16261 gtaagagtaa ctaaattctt acatcattca aacaataatt aagtagatcg catcaggatg
    16321 tttacagaaa caatggagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt
    16381 atacgaagtt atggtctgag ctcgccatca gttcaaaaat gagaagtggg tttctgcttc
    16441 atttatcctt ttagcaaata atattcttag tggatagtag tactaagata gtaattttaa
    16501 tattattgta aaagttctgt tttcaaaatt aaaaaaaaca aagtttcctg aaactctact
    16561 gaagttttta ggttttgctt ttagtagtaa atgggcatag gtcattcaaa tagctttgtt
    16621 tgatctcctc tatcccaaga aattaggcag tttctatgag attaaataca ggattagttt
    16681 gtttttttaa tatataccaa agcctgcaag aactttgcta cttgctggga ttcgttcttt
    16741 taggtgctct ggagtactta gatgtgtgct tattagccct gggagggaga gctacccctc
    16801 acaactctat tttctagtct ttattagcac agaggatctt ttgcatcata attttaatct
    16861 ttgatagata ttttcagtta tagttttagt attttatatg ctgccctttg aaacatggga
    16921 tagcagtttt gatttacaga aaattttaat taaattaatt ttttaaaatt tcatgaatga
    16981 atactatatt gacatcattt ctctcccctc cttctcttcc ctctcccttc aatcctcccc
    17041 atgtctccta tattaaatct tcggactctt ttaaaaaatt attattgtta tacatatgta
    17101 ttagaatgaa tatatgaata caacctgatg gttctgttga ttctgtttag tgttgcttga
    17161 atgcatatgt ttttagggct gactacttgg tactcggtaa ccaatttgga taaaatttgt
    17221 atttgttaac aactgttccc ttttcttctt gagcataaca gtttttatgc cattagcctc
    17281 tagggagact gggagcttca gttgttggaa ttgatcccgt ggctgaaaac attaagatag
    17341 cacagcacca taagtccttt gatccagtcc tggataagag gatacagtac aaagtgtgtt
    17401 ccctggagga ggccgtggat gagagcgcag agtgttttga tgctgtcgtg gcttctgaag
    17461 ttgtggagca tgtgagccac ctggaaatgt tcatccagtg ctgctatcaa gtgctgaaag
    17521 taagagttgg cttctggttg gcttcctaaa gtgtggctcc atagcgatgt attgtgctgg
    17581 ggtactttta gcagttcagc aagtaagacg ggagagaaag gctgacaaag aatgattaag
    17641 gcctgctgtc tctgccagag aggacttttg cccctgagtt accttccata gcaagtcttt
    17701 ccaaagtctg atgaggcctt aatgctgcag tgatctatga cctataaata tttttaaaga
    17761 ttgtaaaatt atgcttatgg gtatgcgtat gtgagtgctg gtccctgcag aagctagaac
    17821 tacctgaccc tcaatcctcg tggatctgga gttacaggaa gttgtggact tcccagtgta
    17881 ggcattggga agtaaacttg ggtcttctgc aagagcaata tgcactctta accactgaat
    17941 tgcctctcta acattggtcc atgtttattt ctttaaaatt tgccttcctt tattttgtat
    18001 tatctctgtg tgtttacttt catgtatgtc tgtttaccat gtttgcattg tcctcagagg
    18061 ccaggagagg gtgtcggatc ccctggaatt ggagttaaat atggttgtga gccactatgt
    18121 gggtactgga cttcaaacct ggggtctctg gaagagcagc cagtgctctt aattagagtg
    18181 gtccctctag cccctgctca tatattttaa agaaagtttt atttcatttt attttatttt
    18241 catttattga aaatagtttt tttcttgcat actatatcct gattatggtt tccctccctc
    18301 tactcttccc atttcctccc cacctctcat cccttccgga cccattccct gtctcttact
    18361 gaaaacaaac agtcttctaa gggacaatta ttatatatga catggtatta gatatgatat
    18421 gatatgatat gatatgatat atgatatgat atgatatgat atgatatgat atgatatgat
    18481 atgataaaac aaaaaactaa cacatcagtc ttggacaaaa caaaaggaaa agcacccaag
    18541 agaagacaca agaatcagaa acatacttgt ttgcacgtgc aggaatccca taaaaacact
    18601 aactggaagc cataaaatct gcacagagga cgtggtgcag acctgtgagt ctctgctgct
    18661 ccatttgctt ttgatgtcct ctgtcgcctc tgaatcttat actcttcctg cctcctcttc
    18721 ctcaggcttt ccgagggaaa ggattggatg gagacattac atttagggcc gggtctctca
    18781 ttttctgtac agtgtctggc tgtgggtctc tgtatttgtt tccatctgct gcagaggaag
    18841 cttctctgat catggctgaa tgaggcactg gtgatctatg agtatagcca gcacagtatc
    18901 attaggagtc atttcattgc catgtttgtt tttttccttg tggttttggt tttagcctag
    18961 ggccctggga tatctagact caggtctttg gctaagtagt gttgtatatg ggttccatct
    19021 tgtggagacg gtcttaagtc aaatcagata ttggttggtt cctcctccag acttagaaca
    19081 aagcatttgt ggttacttag tttggtcttt gcatttctct tttggtttca tgcaaagtac
    19141 atgcctgttt cagagacact agtttatagg gaagaaggct ttatattgcc ccagttagac
    19201 ttctccatct tcagtgagtt gtgtggctgt tgcttgctgt cagtttgtga agagcaacct
    19261 atagtcttgg caacagcctg ggttgttttg ggagtctcat gaaacctctt tggccaacaa
    19321 atcaacaaaa gatgtccatt tagggccctg acttcccccg ttatttggtg attttattta
    19381 gattgctttc atgtgtgtat ttattttagg aaacttctac tgtattatat ttctatttta
    19441 tcccccaaat tgcccctaat tttaactgtc tctccctgtc ttctttcctt tgtccccctc
    19501 tccctgtcac ccactggtcc tcccattcta gccccacccc ctccatccat atctgtctat
    19561 tcggtttccc tttcttagtg agatctatgt gtccctgcta gtcccttact gtatacctat
    19621 cttctctgtt gctaaggatt gtagcttgct tatcatagac ttaatgagaa catagagcta
    19681 atatctatac atttgtcttt ctgcatctga gatacctcct caggataatt tttctatcca
    19741 ttttccattg acctgaaaat tttatgatta cattcttttt aacaggtgaa taacattcta
    19801 gtgtgtaaat gtaccacatt ttctttatcg attttcctgt ttcatgacat ctaggttgtt
    19861 ttcaatttct tgttattatc aatacaatag cagtgagcat ggttaaggac gcgtctctgt
    19921 gataggatga agcatccttt tgttatagct gatcttgagg tagatagact tccatctttc
    19981 tgagggac
//