LOCUS       KO-first_condition_ready_34525_MGI:101805        21701 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Gys1 targeting project(s): 34525
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt1
COMMENT     design_id: 42366
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            5074..5265
                     /db_xref="ENSEMBL:ENSMUSE00000229424"
                     /rank="3"
                     /note="ENSMUSE00000229424"
     exon            6451..6636
                     /db_xref="ENSEMBL:ENSMUSE00000203786"
                     /rank="4"
                     /note="ENSMUSE00000203786"
     exon            6877..7021
                     /db_xref="ENSEMBL:ENSMUSE00000531844"
                     /rank="5"
                     /note="ENSMUSE00000531844"
     misc_feature    4112..8946
                     /note="5 arm"
     misc_feature    8974..8998
                     /note="B1 Gateway"
     misc_feature    9011..9058
                     /note="Frt"
     intron          9059..9933
                     /note="mouse En2 intron"
     misc_feature    9931..9933
                     /note="Splice Acceptor"
     exon            9934..9972
                     /note="mouse En2 exon"
     misc_feature    9973..9989
                     /note="Frame 1 insert"
     misc_feature    9990..10043
                     /note="'self-cleaving' 2A peptide"
     gene            10044..13121
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13122..13175
                     /note="'self-cleaving' 2A peptide"
     gene            13176..13967
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14166..14408
                     /note="SV40 polyadenylation site"
     misc_feature    14425..14472
                     /note="Frt"
     misc_feature    14479..14512
                     /note="loxP"
     misc_feature    9948..10063
                     /note="FCHK std_gt1"
     misc_feature    9948..9965
                     /note="FCHK QC prefix region"
     misc_feature    9966..10045
                     /note="FCHK QC critical region"
     misc_feature    10046..10063
                     /note="FCHK QC suffix region"
     misc_feature    14528..14548
                     /note="B2 Gateway"
     misc_feature    8947..14578
                     /note="Synthetic Cassette"
     exon            14996..15113
                     /db_xref="ENSEMBL:ENSMUSE00000203778"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00000203778"
     exon            15489..15609
                     /db_xref="ENSEMBL:ENSMUSE00000229381"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 2 ENSMUSE00000229381"
     exon            16101..16207
                     /db_xref="ENSEMBL:ENSMUSE00000203777"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 3 ENSMUSE00000203777"
     misc_feature    14579..16419
                     /note="Critical Region"
     misc_feature    16443..16476
                     /note="Downstream LoxP"
     misc_feature    16420..16499
                     /note="synthetic loxP region"
     exon            17264..17323
                     /db_xref="ENSEMBL:ENSMUSE00000203779"
                     /rank="9"
                     /note="ENSMUSE00000203779"
     exon            17411..17489
                     /db_xref="ENSEMBL:ENSMUSE00000203783"
                     /rank="10"
                     /note="ENSMUSE00000203783"
     exon            20530..20643
                     /db_xref="ENSEMBL:ENSMUSE00000229356"
                     /rank="11"
                     /note="ENSMUSE00000229356"
     exon            20885..21011
                     /db_xref="ENSEMBL:ENSMUSE00001205166"
                     /rank="12"
                     /note="ENSMUSE00001205166"
     misc_feature    16500..21701
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg atagagatga agagaggaga accaggggaa
     4141 aagagtctgc ggtgagcaga tccatgaact aacgaggaag gggcgtggct cagaggcaga
     4201 gcccctgcct agaatccccc aatgaggggc taggggtgtg gcccagtggc agagtccttg
     4261 cctagaatct ccctgtaggg gtctggggtt atagctcagc ggtagaaccc ctgcctagca
     4321 tggcccgtgg gtttcatcct aagaaccaca aacagaagcc tgttggtccc aacactacag
     4381 agtaagttcc aggacagcca ggactaggca gagaagccct gtccgtctcc ccctcccccc
     4441 acccccaccc ccgccaaaga aatacaagga gaaagccaga agagagggaa ggtgagatgg
     4501 aagaacccta gaagagatgc agatggaaag acaagtgcag aggctccacc tagtcacagc
     4561 ctgtgttcgg ggttgggttc cagcatccag tcaggccaga ccatgtccct atgagacccc
     4621 agagtggcca catccctccc ccaggacagc tgttcctaat aatagacccc cccctcccag
     4681 caaaagggca gacaacagct gggaggggga ggggtgctcc agtgaggccc tggccagacg
     4741 aagccaggtt gggacagagt tagatcaatg gcctggagcc atacctgtgg tctccggagg
     4801 agaatggcta atggggactg aaatagaaca tcgagtttta ggaaggtggc aattggtagg
     4861 aaagtacagt cctggctgat agacacgtga cacaggtgac aaaacaggcc aatggggaga
     4921 ggagaaaggt agtgggaggg gttacagagg gccacagatg aggccgtggg tgaggggaac
     4981 gtgagacagc tgagctcagt taccaggctg gggatgtaag ccggcgagga atgcgggcca
     5041 ggtcttctga gcctcccttt gcctctccct caggtgtatt ttgggcgttg gctgatcgag
     5101 gggggacccc tagtggtgct cctggatgta ggagcctcag cttgggccct ggagcgctgg
     5161 aagggtgagc tttgggacac ctgcaacatc ggggtaccct ggtacgaccg cgaggccaat
     5221 gacgctgtcc tgttcggctt cctcaccacc tggttcctgg gtgaggtagg cggtactaca
     5281 gaccccacct cagccttcct acccagactt gaaaccatca tcatcatcat caccgcttgg
     5341 gccagagagt gggcttcccc tgggaattct gggaattgtc catccactct tttaatccaa
     5401 ggatcgtctc tgaaaagagt ctagctgctt ttctcagaga ccagaagtgt caacctcagt
     5461 ttcatttcct ctatatccag gtgtaaacac actgcgacat tgtttccagg gagtcctgcc
     5521 agaagagggt tgggtcccag gaattctggg agttgctgtc tactttaata tccattaccg
     5581 ttaagcactc agggtaactt ttgatactta ccaaccttga gttggtttca gaaccagaaa
     5641 tctgggcttt ccccacccca aacccattct agaattttta agagaagttt ttaggagact
     5701 taattgggat gccttaggac aaaaaagagc tacacgtgcg ttcaaggaaa tctgggagtt
     5761 ttggtcctgt tttgtgccca taaaagttct actaccgctg attgaaatgc tgagtcttag
     5821 ccgggtggtg gtggcatgta cctttaatct cgggaggtag agacaagcag atccctgagt
     5881 tcagggcaag cctgttctac aaagtgattt ccaggacagc cagtgctatt acacagagaa
     5941 aatctgtctt gaaaaaccaa aagaagccgg gcagtagtgg cccacgcctt taatcccagc
     6001 actccagagg cagaggcagg cagatttctg agttcgaggc cagcctggtc tacagagtga
     6061 gttccaggac agccagggct atacagagaa accctgtctc aaaaaaccaa aaaaaaaaac
     6121 aaaaaaaaaa accaaacaaa caaacaaaaa aagaccaagt ccctgcagta gtgaccaggg
     6181 cttggggtgt tgatccttcc agttccgcgt tgccagaatt cataccttgc caaaaaaaaa
     6241 aaaaaaaaaa aaaaaatctc attaacctct ggggacaagg tcagccggtt ggcctaactt
     6301 gggttttggc cctttgtgcg accaatgcct gctctgcata atggcgggct tacctctccc
     6361 ctgcttcttc atccgtgatg gggattgctg ctggttcctt ttgggccgtg tggaaacaca
     6421 gtgaccctcc ctgtccctgt tgccccatag ttcctggccc agaacgaaga gaagccgtat
     6481 gtggttgccc acttccacga atggttggct ggcgttggtc tgtgtctgtg ccgtgcccgg
     6541 cgcttgccgg tggcaaccat cttcaccact catgccacgc tgctggggcg ctacctgtgt
     6601 gctggcgctg tggacttcta caacaacctg gagaatgtga gctgcgattg tattgtgaga
     6661 cagcccaaag ccctggcagg gaatcgtgct cctaagagtg caggctccca gcccttccac
     6721 catcagacat gccccccccc ccccccatcc tggccatcct ccctcagacc tgcctctcag
     6781 agcgcagctg ccaatcccaa cctccctaag accccagtcc agtccctagt cttaccctgg
     6841 tccttttaac gctgactttt ctcccttcct acccagttca atgtagacaa ggaagcagga
     6901 gagaggcaga tctatcaccg gtactgcatg gagcgtgcag cagctcactg tgcccatgtc
     6961 ttcactaccg tatcccagat caccgcaatc gaggctcaac acctccttaa gagaaaacca
     7021 ggtagggtct gagctgtggt ccctacatgt ggctgaggta agaggctcta tgtcacactc
     7081 aacaccaatg ccattttact ccagagagta gaacaacttc cctaagtcct ggattgagat
     7141 ggtaactcgt aaatgaagct ggctgactct gtatcctgaa ggataatagg agctatagtc
     7201 tcattcatcc taattttatg agtaactaat ataaatactg atcttctgaa agaaatgcaa
     7261 atatggcggg ctttattttt gaataaagtg gaactagcta ggaacttggt ttactgatcc
     7321 tgcctcacgt gctcctaagt cttggcccta ggtcctgatg agggttgatt tgttgtaatc
     7381 gtgcgtgcgt gtgtgtgtgt gtgtgtgtgt gtgtgtgcgt gtgcgtgtgc gtgtgtgtgc
     7441 gcgtgcgtgt gcgtgtgtgt gtgcgcgtgt gtgtgtgcgc gtgcgcgtgt gtgcgcgtgt
     7501 gtgtgtgcgt gtgtgtgtgt gtgcgtgtgt gtgtgtgcgt gtgtgtgtgt acgtgtgcgt
     7561 gtgtgtgtgc gtgcgtgtgc gtgtgtgcgc gtgtgtgcgt gtgcgtgcgt gtgtgtgtgt
     7621 gtgtgtgtgt gtatgtgtgt gtgtgtgtag aaaccagagg agaatgtagg gggtcttctc
     7681 tctgtgactc tccacctaat tttttaaaga ggtgtttgtt atttttattt atgtatatgt
     7741 gtgtgtgcgc gcgcgcgcat ccttgtgtgg gtacatgcac acatgcatgg tgtgtatgtg
     7801 catgtgtggt gtgtgtgtgt tcacaggggg cggaagaggg cttatatcct aatcttcagc
     7861 tgcagttaca agtactgtta accaccccgt ctgcgtgctg caagagcagc agtaagctca
     7921 tttgactgca gagccggttc atcagaccca acagctcttg taagacaggg tgtctcattg
     7981 aacctgaagt tcaccatttt ggtgaggcct cgtaagctcc cagggcccac ctctcttccc
     8041 ccacccccac actaggatta taggcgaatt tagccatgcc cagcttttat tgaatgtggt
     8101 aggtctgatc ttgggtcttt ttttaaataa actttataaa cgcttctatt ttcatatgca
     8161 ttactctcca tacctcagct cctagagatg ggtagtgcca tggatagtgc catgggtagt
     8221 gccatggata gtgccatgga tagtgccatg gggcctgata tactcaggaa ctctgggagg
     8281 tgcagttttc catctgtcct tctcccattc tgacacaggg actgaaaata ccactgctct
     8341 gcttctccag gatatccact cccctctctc tctctccctc tccctctccc tctctccctc
     8401 tctctccctc tctctccctc tctctctctc tctctctctc tctctctgag ccagggtctc
     8461 actatgtagc tcagggtcat cttgattaac acgctctgtc atacttggct cacagtgcac
     8521 catctgtctt tatgtctctt ttagggactc aaccctttct cccctcatat acttttaaaa
     8581 aatatttgtt tttatgtatg tgtgtgcgtg tgtatggcac gcatgtgtgt gtgcatgtgt
     8641 gcgagtctga gtgtataaac actggttact tttctgggaa atccaggttt aattctgaga
     8701 acacacatgg tggcttataa ccatctgtgt gttccaattc ctggagacct gaccacctct
     8761 tcttgcctcc acaggcaaca ggcacatata tgttacacag tcatgcatgc aggcaaaacc
     8821 accatacaca taaaatacaa gaaaaaaatc taaaacagag tatgaagggt caagtagcgg
     8881 tgttgaatca gtcccccgtg gagctgagat cactaaaggg ccattataaa actgtgacat
     8941 tatgaaaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa agcaggctgg
     9001 cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
     9061 accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg taggaggaag
     9121 gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc actttatgtt
     9181 cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg tctggggtag
     9241 aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata ggagccaggt
     9301 ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa gcctggggta
     9361 ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc cttggctcct
     9421 cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata ggaaggggga
     9481 cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat ctcctctcac
     9541 ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag aaaacaaatg
     9601 caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta ttgggatccc
     9661 ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc acacacacac
     9721 acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata tcaccacccc
     9781 caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc ccagtatctg
     9841 caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc cagcaaccag
     9901 taacctctgc cctttctcct ccatgacaac caggtcccag gtcccgaaaa ccaaagaaga
     9961 agaacgcaga tcgcagatcg cagatctccg agggcagagg aagtcttcta acatgcggtg
    10021 acgtggagga gaatcccggc cctgggatct ggactctaga ggatcccgtc gttttacaac
    10081 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10141 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10201 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
    10261 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
    10321 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt
    10381 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
    10441 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
    10501 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
    10561 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg
    10621 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
    10681 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
    10741 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
    10801 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
    10861 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
    10921 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
    10981 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
    11041 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
    11101 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
    11161 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
    11221 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
    11281 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
    11341 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
    11401 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
    11461 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
    11521 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
    11581 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
    11641 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
    11701 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
    11761 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
    11821 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
    11881 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
    11941 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
    12001 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
    12061 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
    12121 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
    12181 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
    12241 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
    12301 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
    12361 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
    12421 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
    12481 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
    12541 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
    12601 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
    12661 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
    12721 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
    12781 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
    12841 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
    12901 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
    12961 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
    13021 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc
    13081 gctaccatta ccagttggtc tggtgtcagg ggatcccccg ggagggcaga ggaagtcttc
    13141 taacatgcgg tgacgtggag gagaatcccg gccctattga acaagatgga ttgcacgcag
    13201 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
    13261 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
    13321 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
    13381 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
    13441 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
    13501 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
    13561 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
    13621 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    13681 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
    13741 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    13801 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    13861 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    13921 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
    13981 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
    14041 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    14101 ccagcgcggg gatctcatgc tggagttctt cgcccacccc ccggatctaa gctctagata
    14161 agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    14221 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    14281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    14341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    14401 ctggatccgg gggtaccgcg tcgagaagtt cctattccga agttcctatt ctctagaaag
    14461 tataggaact tcgtcgagat aacttcgtat agcatacatt atacgaagtt atgtcgagat
    14521 atctagaccc agctttcttg tacaaagtgg ttgatatctc tatagtcgca gtaggcggct
    14581 ttaatcccag cattctggag gcaggcggat ttctgagttt gaggccagcc agggctacac
    14641 agagaaaccc tgtctcgaaa aacaaacaaa caaacaaaaa aaaaaaaaag aaagaaaggg
    14701 aagtcacctc cccttcggtt tttgcctgtg aagttgatca caataaagaa atgaagcatg
    14761 gggctggtga gatggctcag tgggtaagag cacccgactg ctcttctgaa ggtccgaagt
    14821 tcaaatccca gcaaccacat ggtggctcac aaccatccgt aatgagatct gactccctct
    14881 tctggtgtgt ctgaagacag ctacagtgta cttacatata ataaataaat aaatcttaaa
    14941 aaaaaaaaaa agaaagaaat gaagcatggg cccggcctgt ctctcccaat cctagatatt
    15001 gtgaccccca acgggctgaa tgtgaagaag ttctctgcta tgcacgaatt ccagaacctt
    15061 catgctcaga gcaaagcacg aatccaggaa tttgtgcgtg gccattttta tgggtatgtg
    15121 agccagacag tgaagagagg tggatggggg cgggggagag gtgagattcc tgcgtctgag
    15181 gaggagggct gtggttagga ccctgagctc tgcggagaga agactcccga gtctcaggga
    15241 agagagtgag gattccttgg tcctcagtcc tggacagcaa tggtctccat tatttcctga
    15301 tatctggagc catgggatgg gtattagggt aaatactcat ctaaggagaa attctgcaat
    15361 tgcatgcatc cctctgatac ctgtgcacta taaaaacttc ctttggctct gtgacttctg
    15421 gggtttgatg gcttcagcac agggttaggc gcttgggcct cacactcact cccctgtggc
    15481 ttctttaggc acctggactt caacctagac aagactttgt atttctttat cgctggccgc
    15541 tatgagtttt ccaacaaggg agctgatgtg ttcctggagg cattggcccg gctcaactat
    15601 ctgctcagag taaggcctgc tcggtgggac agtgggtggg gagccggagg gggaagtgtg
    15661 tgcctagatg gggagaacag agacccgaga gatggctgtg gaaacccttg gcagagtata
    15721 gagtcccaga aagatcagaa gtgagatctg gtggcctgta cctgttatcc cagcacctgg
    15781 gaggctgaag cagaaggatc aaaacttcaa ggtcagcttt tgtctataga atgagttcaa
    15841 ggttgacctg ggcaattttg agacgctgta tcaaaataaa aaccagtctg gcagtgtggc
    15901 tcagtggtag atcccctgcc tagaatcgcc cagtgagggg gcaggagagt ggctcagtgg
    15961 taaagcgcct acgtattcta gagatttgat tttaatcccc tatattatta tgaaaaagaa
    16021 aatcggaagt cccatcattc ttagcagcaa aggcatgatt gagtccacaa ccctgatgtc
    16081 cctctcatcc ccgtccccag gtgaatggca gtgagcaaac agttgtcgca ttcttcatca
    16141 tgccggcccg gaccaataat ttcaacgtgg aaaccctgaa gggccaagcc gtgcgcaaac
    16201 aactatggtc agcaatcctt gtccaggacc ctgtgggctt cgtacctgca gtcgccatat
    16261 cttatctctc tctggctatc tctgacatga tcaggattcc tagtgaactc ctctcttaaa
    16321 gcatgtggcg gggcagacaa gtgtgagcgc gtaatcaggg cagccatcac acagtgaggt
    16381 gacctgactc cttgttatct tgctgaacca ccgaatcctg agatggcgca acgcaattaa
    16441 tgataacttc gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcat
    16501 ctgaaaccca cccgttcact gcccaccagg ctctgaatat ctaccacgag gcaggcactg
    16561 ttctaagccc tgggaacagc agatgcatgt ttaagaaatg aggaagagct gaaaatgtag
    16621 ctcatggtag ggcgttcagc actcacaagc cccggcacgg cataaagtgg gtgtgatggt
    16681 ccatttctct taattctaat acttggggag tggaggctgg agggtcaaaa ggtcaaggtc
    16741 atactgggca gcatactgag ccaggaggca gttagagaga gagagaagag actggtgtgg
    16801 ttgggtcggc atgtccctgg gaggatcgta agtcatgaga cagtagggtg acagtctttc
    16861 cgaacttgtg tgccagggtg ttcctctcag aatattattc ctcctgttta taagacaggg
    16921 cctagcagtg ctctgtccaa gactcattct aaccctaata atagctgaca tttctggaag
    16981 gctctctgtg tgctgggctt tccctgacct ctcggactcc tctgcaacca cctgaggttg
    17041 actctatttt tacactcatc tacaagagga gtcactgacg ttggagagga aaggccacca
    17101 ttcccagcca cctcaaggaa ctcaagcagt ctccaggtcc ccatcttcca gtttgaggtc
    17161 ccccggggtg tggattctga acccagacgg aagtttctct ggtgcccaca atgcctgaca
    17221 gaacatagtg gctgtgatgt gacattgttt ctctccgttc cagggacaca gccaatacag
    17281 tcaaggagaa atttgggagg aagctctacg aatccctttt agtgtgagtg tcagccccta
    17341 gctcttctcc catgattgcc ctgacccatc ccgtcacttc attctgaaat gcctccccct
    17401 ccccttgcag ggggagcctc ccggacatga acaagatgct ggacaaggag gacttcacta
    17461 tgatgaagag agccatcttt gccactcagg tatggtcttt acccctgagc aaagtgctgg
    17521 ccagttctgg catttgctga ggcctcttgc tgcaaaggtt tctgggagtt ctgttctctt
    17581 tggtggccct ttacagaggg tccagttctg aaatccagag aaataccgat agtaaaacac
    17641 acccaccaca tcacaaaggg taagatggtt tattccggaa ccaagtataa gcatggattg
    17701 ggaacacaga ttccggttac agtgttccaa cacagtgacg atcgtgtggc tttttatgat
    17761 aacagaacac tgacagtaat gaatgaaggc cttgttaaaa tacattgctg gaggggatac
    17821 cagggggtcc ccttctcaga ggagaagggg tgagggagag atgatgaggg gagaatggga
    17881 ggagaggggg ctgatattgg gatgtaaagt gaacaaataa attaatttta aagagacact
    17941 gctggggctg gagagatggc tcagtggtta agagcgccaa ctgctcttct gaaggtcgtg
    18001 agttcaaatc ccagcaacca catggtggct cacaaccatc cataataaga tctgatgccc
    18061 tctcctggtg cgtctgaaga cagctacagt atacttacat ataaataaat aaatctttta
    18121 aaaaaagaga gagagactgc tggagacatc aggctgagaa ggggcacaga aagggggaac
    18181 ctcagctcta tgtctggggg catttccagt cttggtgaaa actagggttt cttttttttt
    18241 ttttttttgg tttttcgaga cagggtttct ctgtgtagcc ctggctgtcc tggaactcac
    18301 tttgtagacc aggctggcct cgaactcaga aatcagcctg cctctgcctc ccaagtgctg
    18361 ggattaaaga agcgtgcacc accactgccc ggcttaaaac tagggtttct ttaagttcct
    18421 gcacccctaa aggttttaag tcacagccct tattagagac aggtagcaaa gagtggcggg
    18481 cgattcagga gcactgaact agaccaagat caatttttgt tgttgttgtt gttggtggtg
    18541 ttttgttgtt gttgtttgtt ggtttgtttt gcctttgcag acagggtttc tctgtgtagc
    18601 cctggctgtc ctgaaactgt ctctgtagac caggttggct ttgaactcag agatccacct
    18661 gtttctgcct ttggagtgct ggtgtgtgtg ccaccccacc tgcccttatt tttatgagac
    18721 agagtctaat tagactctct acctgccaca ttccagcatc tccacagtca gtcagccttg
    18781 cagaaggttg ttctctgtta aggtacattt ctttttgggg acttcatagt ttattgaacg
    18841 ttcagaagcc cccaggtgac aacttctgtt tctgctgccc ctggggcgtg ttaagggtag
    18901 cagtttaagg ggcctttagg agataggagt caggcaagtc aatacaccta gtgttttctg
    18961 gagaacactg aatgctcatc agccactatt cgccgtccta gaactcactc tgtagagcag
    19021 gctagcctgt aactgagatc tgcctacctc tgctttccga gtaccaatat taaaggcatg
    19081 cactaccaag cccagctccg tggctgtgtc ttacctctca cagaaccctc tgattcctgg
    19141 ggttggagtt aaaaggacag gaaacattag ggagatgtgg ctcacaaaat gaaaactaca
    19201 actcccatag tattttgtat tatattgacc ttggcccctg aggcttttgg gagttagagt
    19261 tctttttttt ttcctgtggg tcaaggggct ggtgcctatg tttggcatta atgaggctga
    19321 gttttgggat ctagacagcc agcatggatt aatgaggtag aatgtgtttt tgggcaaaat
    19381 atttttgaag ttaagcagcc agctttactc atgaagaagc cttttacatg gggctgctgg
    19441 gagttgtagt ttttcaccct cataccaccc aggagtgggc ccattaataa cataatgatg
    19501 gaacagttgt aaagaacttg tcataaaaga gagggctggg gggctggaga gatggctcag
    19561 cagttaagag cattgactgc tcttctgaag gtcctgagtt caaatcccag caaccacatg
    19621 gtggctcata atcatctgta atgaaatctg acgccctctt ctggtgcgtc tgaagacagc
    19681 tacagtgtac ttacatataa taaaaaatta attaaaaaaa aaagaagaag aaatgtcttt
    19741 taaaaaaaaa aagagggggc tgggatttcc tgtctgtctt tgtgttgcag cggtgacttc
    19801 tgaaattgtg cttaccttcc ctgagggggt agggcagtgg ggttggaagt gatggtggtg
    19861 atggtagggt gtggattttc ataagaaaac tacaacttgg gcctggagag atggcgcagt
    19921 ggttaagagc actggctgct ttttcagagg tcaagagttc aattccagcc gggcagtggt
    19981 ggctcacaac catctgtaat gagatctgac gccctcctct gctgtgtctg aagacagaga
    20041 gtacttacat attaaaaaaa aaaaatcttt tttaaaaaaa aaaaagaaaa ctacaactcc
    20101 cagctgtcct tgtggcttgt tatcaaatag acatagctat acacttggct ggggtaggta
    20161 cagttcttta gagctcccca cccccaactc ctccccaccc ccccaccacc ggcagagggg
    20221 agggaggagt ggagtcaaca ttctgatgtc attttggttc aagttacgtg gttgcttcca
    20281 tcttggtaag gttctttgag tttttgttat tgacgggcaa gactatataa ctaaacacag
    20341 acatttcctc acaagatgtg gagtgcctct gtttagctgg gatttttttg ttggtttggt
    20401 ttagctttgc ttgtgatagt taaaagtcgg gctccagtca ggagtatgct gacctctgct
    20461 ggtagcttct tcagagccct gtgaccgccc tccctcccct gacccctccc tcatgtctcc
    20521 tgtctacagc ggcagtcttt cccaccagtg tgcacccaca acatgctgga cgactcctca
    20581 gaccccatct tgaccaccat ccgccgaatt ggccttttca acagcagtgc cgaccgtgtg
    20641 aaggtcaggg ctggcccgag gttggtgggg tgtggctggg gaaagggttc ttggatatca
    20701 tgcttctcct ctacctagtc tgtcaattgt actgtttaga aagaaccctg gcttctgaag
    20761 ccaaatgaat tcagtttaag tctttagctt gttgtggact tgctgtgtgt ttctagagaa
    20821 atgtaccctc tctgggctac tctaggtggc tagaagtagc tcagcccttc tcctctgtcg
    20881 acaggtgatt tttcacccag aattcctttc ttccacaagc cctctcctcc ccgtggatta
    20941 tgaggaattt gtccgcggct gtcaccttgg ggtcttcccc tcctactatg agccctgggg
    21001 ctacacacca ggtgagtaca ggaggtgagg ggtggcggat tgctgggtcc tgttcagttc
    21061 tggcatgaac ctgcaacacg gttcctctgt ttcaagatga tggtagtgac ccatcctgta
    21121 tccatgtgtc acatgtttgt ctcacagaag gctacagagc tcagactaga tgactgatgt
    21181 tcatttccgg tcgtcgacac tgataacctc agctccaatt tgctgatgtt ggagttgcac
    21241 atgaaccagg ccttccccgg tgtctctcag acttgctatg tagcccagac tggtcttgaa
    21301 ctcctgatct tgtttccaga ttcggagtgc tacaccactg tgatctgttt gtgcagtgcg
    21361 ggctgtagaa gctggggctc tgtgcctgcc aggcaagccc tctaccctct gagcttctcc
    21421 tcctggtccc tagtgctgct caacctcttc ctctcctcac ctctcttcag ccctgagctt
    21481 cgagttagaa ttgcttgtgt gtaattctcc ttcaccgaga ggatcctgtg gggcttttcc
    21541 tccttatttt tgttttttag gcccgcttgt tttctgatac ccaagctggc ctccagctca
    21601 caggtgtctg agggtgacct tgaactcttg acctccctgc ctctaccttt ccttgtattc
    21661 tagacatatt cttcaattct gcactcccga gcacacactc a
//