LOCUS       KO-first_condition_ready_77476:79276_MGI:101791        23204 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Isl1 targeting project(s): 77476:79276
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 88605
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            8751..8940
                     /db_xref="ENSEMBL:ENSMUSE00000305526"
                     /rank="2"
                     /note="ENSMUSE00000305526"
     exon            7526..7819
                     /db_xref="ENSEMBL:ENSMUSE00000305534"
                     /rank="1"
                     /note="ENSMUSE00000305534"
     misc_feature    5434..11309
                     /note="5 arm"
     misc_feature    11337..11361
                     /note="B1 Gateway"
     misc_feature    11374..11421
                     /note="Frt"
     intron          11422..12296
                     /note="mouse En2 intron"
     misc_feature    12294..12296
                     /note="Splice Acceptor"
     exon            12297..12469
                     /note="mouse En2 exon"
     misc_feature    12481..13074
                     /note="ECMV IRES"
     gene            13075..16135
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    16174..16417
                     /note="SV40 polyadenylation site"
     misc_feature    16421..16454
                     /note="loxP"
     promoter        16481..17000
                     /note="human beta actin promoter"
     gene            17026..17820
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    18020..18261
                     /note="SV40 polyadenylation site"
     misc_feature    18278..18325
                     /note="Frt"
     misc_feature    18332..18365
                     /note="loxP"
     misc_feature    12311..12388
                     /note="FCHK std_gpr"
     misc_feature    18381..18401
                     /note="B2 Gateway"
     misc_feature    11310..18431
                     /note="Synthetic Cassette"
     exon            18794..19053
                     /db_xref="ENSEMBL:ENSMUSE00001266345"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001266345"
     misc_feature    18432..19207
                     /note="Critical Region"
     misc_feature    19231..19264
                     /note="Downstream LoxP"
     misc_feature    19208..19287
                     /note="synthetic loxP region"
     exon            22479..22646
                     /db_xref="ENSEMBL:ENSMUSE00001286750"
                     /rank="5"
                     /note="ENSMUSE00001286750"
     exon            20925..21211
                     /db_xref="ENSEMBL:ENSMUSE00001271145"
                     /rank="4"
                     /note="ENSMUSE00001271145"
     misc_feature    19288..23204
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggaacattac taattgtttt ggtgaatgcc
     5461 tgtatatgtt tggaagggtg aagagtgagg gtcctttgtg ggtctcaagt cactagactg
     5521 atgcttcttt tagatgccac attataaaca tagcgtgaag aaatagaatt ggttcagatc
     5581 ccctatttat ctttgttgtt gtttgttgtt gtttttaaat catggtcaac ctggctgtat
     5641 agaagggtag catgcccttg tacggaggga actgcttctc tgggcttggc aaaagcagct
     5701 tgacaaaaga taccacacat aggatgtcag agagcagaaa ccggggttga aaggaaaaaa
     5761 cacaaccaac cggtaaagtt aaccggagtc tacacaactt tccatcctgc tcacggactt
     5821 caaaataatt tataattaaa aaaaaaaaaa gaacaggaag ggaagagatt cccatctgcc
     5881 ttcttaaaga aaacagcaaa acaagaaagg ggggaaaaac ccactcctgc ttcttaacct
     5941 cttttctctc tgtgtgtact ctgaaaagtc acaggctttc taactcggcc actgcggcct
     6001 aggtggggtt cacaggtttt atcatggttt agatctggtg gaagaaacac aaaaaggagt
     6061 tccctaacct ctggctcttc taagaggaaa acaaaacaaa acaaaacaaa caaacaaaaa
     6121 aaaaaacaag ctgaaccctt ctacgattct gtttgtagcc tctctctctc tctctctctc
     6181 tctctctctc tctctctctc tctctctctc tctctgatca aagtccctct agaagctccg
     6241 gcagaagtgt gggaattctg tgtgtcccct aataactgcc tctgaaagac tgtagatttt
     6301 ggggaatcag gaaagaaatt attggcaaaa gttaccggtg agatgcagcc aacatctcct
     6361 ccagatggct tccccagagg aaacaaatta gaaagctaac ctcaggggag ggaagtaggc
     6421 gccccactct tcttttgcaa cagaccctcc ctgctcacgc cttcatacta aaagatttaa
     6481 aagtctctag agaatattgt aaagttgcgc ttataattac caagccgttt aagggcccag
     6541 tgcacctttg ccttaaaaaa gagtggacaa cagatccata gacagacatg gacaacccca
     6601 ccccaccccc gtaaacttta gggtcctgca ctctgactct agattagaac actttcatgc
     6661 tagacttaaa attgaacagg agacctcacg ggtcgggagg aaaggaacca acctcggagt
     6721 taattcgttt taccttttcg tttgaccttt gcgtttccaa cccgattgtc cgctagggag
     6781 acaagctccc ctttcctccc accccaagtc tttggagagc tcagattggg gagtaacagg
     6841 gtcaacacgg tagcaagaaa aagaaaaaaa gaaagaaaag aaagaaagaa agaaaaaaaa
     6901 gaacacaaaa acgaagtcat aaaaaaaccc atttcttttg ggtctaaccg tcctactccg
     6961 ggtttgtccc ctaacgcgta gcgcgctgct agctccagca gatgcgccct tcccgcaggc
     7021 agcgaaattg gccagccctg ggctgtgcgc gccagcacgg gagtcagtga aggaaagcgg
     7081 cccgttccaa gtgcccccct tttcctggag cccggtaggg acccgcttca caggaaggac
     7141 ctacccacca gctactcgcc acgggcggca gaaaagggag ccccgagctc agcgcagccc
     7201 aaggtccgcg gggagcccgc ggcgctggaa ccccaggaca cgacgcggcg cgtccgggaa
     7261 ggcgcgggga gggggtggtg gacaaggagg cggcggccgg ggacccgggc cacccggcgc
     7321 ccaaggagcg acgtggcctc gggaggctta atcctctcct gcgggctcgc cgagcgggca
     7381 gtggcgcgcg gaggcgccgg ggctgaaata tgataatcag aacagctgcg ccgcgcgccc
     7441 cgcagccaat gggcgcggcg ctcgcctgac gtccccgcgc gctgcgtcag accaatggcg
     7501 atggagctga gttggagctg agaagtttga gtaagagata aggaagagag gtgccccgag
     7561 ccgtgcgagt ccgccgctgc tgctgcgcct ccgctctgcc aactccgccg gcttaaatcg
     7621 gactcccaga tctgcgaggg cgcggcgcag ccgggcagct gtttccccca gttttggcaa
     7681 ccccgggggc cactatttgc cacctagcca cagcaccagc atcctctctg tgggctattc
     7741 accaattgtc caaccaccat ttcactgtgg acattactcc ctcttacaga tatgggagac
     7801 atgggcgatc caccaaaaag taagaagcgg tttaaccttg tggggcttgg tgtgctgttg
     7861 tggtgtgggg gggtctctct caggcaccga tgacagggct ctttggattt gtagtcactg
     7921 cctggagaga cagaagagaa ggtggtcctt ttgttgttgt tgttgctgct gctgttgtgt
     7981 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gttttcttct
     8041 tgtcacgctt gcagcttgca tgtttggtgt gaattccgat ctttgagaaa ctgatcatac
     8101 cttgtgtttg aatgctctgt gccctcccaa actctagccg cctgcccggt gcttagcggt
     8161 gatttcctcc tccggattcc ggagctcttg gagaagttat tatgtagcaa aggtctgcct
     8221 gcacaaacca caccggcaga tatgaattag cattagactt gcaaaagaga gcgagtgaca
     8281 actgtattga tgtctactct tctaacaata tccattcccg cgtgctattg aagaacgtgc
     8341 ttcacggaaa atatagacat cccagcgttg ctttaacgct tctagtcaaa accttttctt
     8401 tgacatgact tatccataat cttgcctaat gattatagca aagagggagg agggagaaaa
     8461 cacaaaatga gcgggtttga ttgcgtgcta agcttacaat gtagactatt caaatctgcg
     8521 ttttacatat attccacctc cctttttaaa aaagcgagtc aaggttttga tggcacactc
     8581 caattaccat cccaaagtgc aatactcttt aagggaagaa caagaaaaaa aaaaaaaggg
     8641 aaagaaaaaa attcttccag aatacaccct ataagagaac gacactaaaa gtgtgtttat
     8701 ctctgtagga agtaaacggt tagtcaatca tgcatttatt ttcatttcag aaaaacgtct
     8761 gatttccctg tgtgttggtt gcggcaatca aattcacgac cagtatattc tgagggtttc
     8821 tccggatttg gagtggcatg cagcatgttt gaaatgtgcg gagtgtaatc agtatttgga
     8881 cgaaagctgt acgtgctttg ttagggatgg gaaaacctac tgtaaaagag attatatcag
     8941 gtatgccatt tttacagggt tattttctga gatttcttgg ctctccttgt ttattatttg
     9001 atgtgccttt gttttgtttt gttgttttta gagggaggga gaacgtttac tttagagtgc
     9061 ccagtgtaca agaaatgttt ttaaaattaa actgttgttc atttcatatc tttggggggt
     9121 ggggtgtcgc tgtgtttcat aaatttttgt tgataaggtt tgttaatgac tctttgaatt
     9181 tttcatacaa aagattattt ccctctttaa acttccctaa ggacaacaac aaaattaagg
     9241 caaataagtt tagggtttca aactttaaaa attctatttt cactgtatag tttatcatct
     9301 tatttatttt tctcctgttt tatcaatgac ttacattatt ttgggggctc caaataaaca
     9361 gacaaaagtt tcaccaaagg atttttgaag aacaagtaca aacattgctc cctttacttt
     9421 tggggtgact cttcctttga taaaaaattt taaaaccaaa ataaaatatt ccttaacgcc
     9481 ccccccccaa aaaaaaccac cggaaattta atctttttaa atattaaccc tttggtgata
     9541 tctaactgcc ttttctttct tatcttatct gggctgatga attagacaga gcagatcaaa
     9601 ttgcccatca tctgtctgcg aacaattggt atatttaggt aattgaacag cttcctttct
     9661 cacattaaaa tctagtaact gataaaatga gggaatcagc tgacggacaa gaccaaaacc
     9721 agggaggagg atctgagctg gcttttgttg cacttttttt tttttcctaa tttcaagttt
     9781 ctctgaccta ccagtttata tcagaaactt gactttctac cttagaaagc ctggtagaat
     9841 ttctaaagga aaatgtgttt gttctaatac aaagcaagat actgttcccc ttcactgtta
     9901 ggctggcgat ggcagaaatg tagccgattt tagatttcag aggtggccat tggcaagtga
     9961 tcggcttttc ctaagccata aagcgctttg gagcttcaga actttcatta aagtttagtt
    10021 aagatttaaa atacagcccc accagttttt ctcctgctgg aataatgccc ctccctcggt
    10081 tttgttttgt tttgctttaa aaaaaacata gattggagga tatattcatg atagctaaaa
    10141 ttaaacaata tactagggag ggtttgcttg tttgtttgtt tggtttggtt ttaatgacaa
    10201 ggagaatggg aggaagtggg atacaggaaa ggggagggat ttcgtcaatg gtttgggaac
    10261 ccggcaaagt aagtcttcct tctataaaat ggatgatctt gcattggaga ggcagaaggt
    10321 aggaaaaaaa atttcaagtc tgtcttccaa cctctgagaa ataatccctt ttcatctttt
    10381 acttggctgg tgtgggctct cgggacactg gagagggaga agggagaacc tactttgaaa
    10441 ctccaaaagc atctttacgc ctctgaagat actgtttccc tcccttgtat acagacttgc
    10501 ttcattccgg atctgtcaat ttgcagatgg agtcactcag ttacttttcc ttttctcttc
    10561 tcttctcttc cgcctgggag catgtttgta agtgtgtgtg tgtgtgtgtg tgtgtgtgtg
    10621 tgtgtgtgtg taacacccat gcacaaccct tttggaggat ctatagttgt aaaagcaggg
    10681 tagagtgacc atttttattt taaggtctct cattggggaa gcaacatgcc acctcctttg
    10741 tgtggatctt gtcctatctt ttttcactct ctatgtagaa attactgata gctattgagg
    10801 gatatccagg caatgaagca gtggatggac tataaggact ataaggactg agcggtggta
    10861 aggaaaggct gacaggggag gaagctttta gcagtgcggg ttggttttgt tgtttttttg
    10921 tccatttgtc ttgctagcct tgtcccccag gcaaactgaa gtagaggtac atgctttagg
    10981 aaccgaggaa agactggttg gaggaacaga agctgggcat taaaataaaa gaggaagaat
    11041 ggagaaagaa ctttccatta acaagagccc cgtggaaaat ctttagttgg ataaatactg
    11101 gaaggccttc actggagagg acctggaaac tctctagtgc ttcatcttgt tattgcgttc
    11161 tgggattttg tgtgttcaga tggagtgggg tgaccctcag tccccttccc cacctttgtt
    11221 gttgatggtg atgatggtgg tggtggtggt ggtgtgcttt ttcagaaata cttttagtag
    11281 cagaaattga gggatagaga atggaaagca aggcgcataa cgataccacg atatcaacaa
    11341 gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc
    11401 tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac
    11461 actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc
    11521 tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga
    11581 ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc
    11641 cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca
    11701 gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc
    11761 accaggcctc agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt
    11821 tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt
    11881 cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa
    11941 aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt
    12001 ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat
    12061 ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag
    12121 cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc
    12181 ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata
    12241 agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggtcc
    12301 caggtcccga aaaccaaaga agaagaaccc taacaaagag gacaagcggc ctcgcacagc
    12361 cttcactgct gagcagctcc agaggctcaa ggctgagttt cagaccaaca ggtacctgac
    12421 agagcagcgg cgccagagtc tggcacagga gctcggtacc cggaagatct ggactctaga
    12481 gaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
    12541 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
    12601 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
    12661 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
    12721 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
    12781 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
    12841 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
    12901 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
    12961 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
    13021 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aaccatggaa
    13081 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    13141 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    13201 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
    13261 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
    13321 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc
    13381 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
    13441 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
    13501 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
    13561 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
    13621 atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
    13681 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
    13741 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg
    13801 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
    13861 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
    13921 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
    13981 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
    14041 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
    14101 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
    14161 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc
    14221 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc
    14281 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg
    14341 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc
    14401 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct
    14461 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
    14521 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
    14581 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
    14641 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
    14701 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
    14761 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
    14821 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
    14881 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt
    14941 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat
    15001 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt
    15061 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg
    15121 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc
    15181 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc
    15241 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat
    15301 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca
    15361 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt
    15421 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg
    15481 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg
    15541 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg
    15601 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt
    15661 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc
    15721 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat
    15781 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat
    15841 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat
    15901 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa
    15961 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc
    16021 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa
    16081 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
    16141 ccgggcaggg gggatctaag ctctagataa gtaatgatca taatcagcca tatcacatct
    16201 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    16261 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    16321 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    16381 tccaaactca tcaatgtatc ttatcatgtc tggatccgga ataacttcgt atagcataca
    16441 ttatacgaag ttatgtttaa acggcgcgcc ccggaattcg ccttctgcag gagcgtacag
    16501 aacccagggc cctggcaccc gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc
    16561 aagagatgtc cacacctagg atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc
    16621 cgggggcagg cctggccatg cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc
    16681 cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct
    16741 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
    16801 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
    16861 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta
    16921 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
    16981 agcctcgcct ttgccgatcc tctagagtcg agatccgccg ccaccatgat tgaacaagat
    17041 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
    17101 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
    17161 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
    17221 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
    17281 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
    17341 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
    17401 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
    17461 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
    17521 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
    17581 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
    17641 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
    17701 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
    17761 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
    17821 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
    17881 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    17941 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccccggatc
    18001 taagctctag ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg
    18061 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    18121 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    18181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    18241 tatcttatca tgtctggatc cgggggtacc gcgtcgagaa gttcctattc cgaagttcct
    18301 attctctaga aagtatagga acttcgtcga gataacttcg tatagcatac attatacgaa
    18361 gttatgtcga gatatctaga cccagctttc ttgtacaaag tggttgatat ctctatagtc
    18421 gcagtaggcg gagactggta ccagaggctg gttcgaggtt tagaatccat caataaattt
    18481 tccttggttt gggctgagtg cctgaactaa gaggtacaaa cttagacaga cacagaggtt
    18541 caccataaag gttccccttg ttaaagggga ggttaaggaa gaaggaggga gaggaagaga
    18601 agggggagga gggaggaagg gggtggggtt gtttctatat tctgcccaac tttgctattg
    18661 ctattttttt ttccaggcag gtcaggttta tggcccgggt ctctggccat cttgggacag
    18721 aacctgcctg aaatcaccca tttttgtgtg tgtgtgtgtg cttttcttcc catgccgggg
    18781 ccggttcatt caggttgtac gggatcaaat gcgccaagtg cagcataggc ttcagcaaga
    18841 acgacttcgt gatgcgtgcc cgctctaagg tgtaccacat cgagtgtttc cgctgtgtag
    18901 cctgcagccg acagctcatc ccgggagacg aattcgccct gcgggaggat gggcttttct
    18961 gccgtgcaga ccacgatgtg gtggagagag ccagcctggg agctggagac cctctcagtc
    19021 ccttgcatcc agcgcggcct ctgcaaatgg caggtactcc tccacccaga ggctgagaaa
    19081 aggcaggcgg ggctaaatca taacctttct ttcctgcaac ctaggggtca cacagtggtg
    19141 cagaaagtgg cctgcagtaa accacacgtg ctctttgcct ttctagcaaa tttccataca
    19201 ctcgtcggag atggcgcaac gcaattaatg ataacttcgt atagcataca ttatacgaag
    19261 ttatggtctg agctcgccat cagttcaact gtactgtcag ctttactttg gcctctaacc
    19321 catagcttac tgaaaatggg cttcagctct ctgccaggtt ttctcctagc tgccgcctaa
    19381 ttaaaggggt ggagccctag gtctcgagga gtttcctccc tagccctgcg aaggaagctt
    19441 aagcagcccc aaggcattta gcttcccacc tctctctctc tctctctctc tctctctctc
    19501 tctctctctc tctctctctc tctctccctc tccctctccc tctccctctc cctctccctc
    19561 tctctctctc tctctctctc tctccccccc ccttgggatt tactttccaa tccaaaagct
    19621 ttttaaaatt tatttttatg cctttgtcaa aaaacaaaac aaacaaacaa acaaaaaccc
    19681 ccatgtccct aaaagataca gtgtcagtaa ctgcaggcac tgcacaggac tgtaacttaa
    19741 aactgtcaac agcctaatct gcagtaattg ccttttaaaa aggagcctgc ctctttaagc
    19801 cattcattct acgtgatttg gtgagaattt catctccaag cctgtagctg tagctctaaa
    19861 ttgaccccat aggtgtttgg cctgacccca ggggggcgtg gaaggtgtgg gatttgtacc
    19921 gtggagatca gaggaccagt tgcccataaa ggaaagtgga aacacaaagg ttggctaaaa
    19981 ccaagaccgg cagacagcag aacattgttt cctccctgcc ttccagtcag aagcaggtct
    20041 ccatccctcc atccccgtta gtataaagcc atcagctcct actaaatacc agaggtgaga
    20101 aaatgacagc ttttccccaa cccccaactc acctcttcta gctgctctgc aattcttccc
    20161 tgagtgatgt ctaactagca gacaagggat aaagccaagg gtgagaaacg gcagaagagg
    20221 cagaaatctt gggagggaag ggaaggaccg tctccactcc tcagtcatct caagctcaga
    20281 cagttcaaca gagtttcagt tttcttttgc ttggggtcag cgggtctctg gaacatccca
    20341 cattgttgat ttagtactaa gaaagacagg cggaccattc gggtagttgg aatccagcat
    20401 ttctttagct tctaaatatg attgtcccct agaactgaag ttcaaggctt tctggcataa
    20461 ttatctgact tttggtcgca gacttcccag ccctgtcgtg tctctagctg tcactggggt
    20521 gttctgcgtt ttattttcag gtaccctcta tgactttaat ccagaaggga aaggcaagcc
    20581 tggaggtggg ggtgtaatct gtgaccttct tctggttatg agggaagggg ggagggagag
    20641 attttactgt tgaaaaaaaa atgtctctgg tgcgggtgga gacagacttc tttcggctaa
    20701 ttgtgtagtg aatggggagt aggaacaggg gttgcatctc ttatctcccc cattactctc
    20761 tgcattgtct tctattgact aaggagcgag agatcttctt cctcctcatc cctaccctct
    20821 gctgtttctg cgaggtgtgc tgagccaggg ctagataggg aatggagtgg gctgatggtg
    20881 gcgggagtgg ccttgcatgc tcaccgagtc cctttgcccc gtagccgaac ccatctcggc
    20941 taggcagcca gctctgcggc cgcacgtcca caagcagccg gagaagacca cccgagtgcg
    21001 gactgtgctc aacgagaagc agctgcacac cttgcggacc tgctatgccg ccaaccctcg
    21061 gccagatgcg ctcatgaagg agcaactagt ggagatgacg ggcctcagtc ccagagtcat
    21121 ccgagtgtgg tttcaaaaca agcggtgcaa ggacaagaaa cgcagcatca tgatgaagca
    21181 gctccagcag cagcaaccca acgacaaaac tgtgagtagc cccgaagcta agcaaggaag
    21241 ggatgctagg agaccttgga agaccgggct agcggggcat ccctgtgatc tgatggattg
    21301 gtactctgtg gagcaccagc aggagcccaa accaaggtgg acctctcact tcacctaaac
    21361 ccaggtgtat agtgtctact ctctacaaga aggccgagat ttggctggtt agaaactgca
    21421 gacgtctgag tggtgcccat cctcccccat ccattgctga ttccaaaagg ggtcatggtc
    21481 ttcatcacca tgggcaccct ggaacctaaa ggacctagtg gctccctccc tcccagggca
    21541 gatggtgcga ggtcttgaac tcaggttctg caagcattgg caccattaga gttgctcccg
    21601 ttgtgaaggg caacatcttg agtgctgatc tagaacatga ttcaatatac tttccatcta
    21661 tccagcattc tggtctcacc ccaactcttg gcaagacttg ccactgtttt tgcatttggg
    21721 agatcccttc aaggtgccca tgcagacact cccctcttcc aagcttcctt ccagcctttg
    21781 gtatcattat gttaggcaac aacacactcc ccttccaccc ccctccccca cctcttgctt
    21841 aagttaaggt aaaggttttt tgtttttttt ttttaaacta cttcagccct atttttaaat
    21901 gccatttttt aaaaaaaatc cattgtcatt aaacttggca ggcagaccga gactgggtaa
    21961 gggtactttc taagttagag cataatagca cagaagtagc cagacccaga aaccttgggg
    22021 ctagggtggg gtaagtatga ttctggaagg aagagtgctt aggggcaggg ggatgggttc
    22081 agctaactca aactgcctag aaaaccagta tcagaaggct gaagggagac agaccctggc
    22141 tggacctcag ttttctagtt ctgggagact gtggcctctt aacgcaggtg ggaggggttg
    22201 gggtagggca taagctgtga aggtgaagca aaaggtgata gatgcagcag aattgtttta
    22261 ataagatctc agccagtctc ataatccact gcacagaagt agaaaaacaa aactgaactc
    22321 aaactcatat ccataggtac agcagattaa ctgagtcaat acagggccac tgtgtagata
    22381 agataaatac ttgggtatat ttacttagca catgcagacc aggaagggag ggagtttgct
    22441 cattaacatg ttgggatggg ggggggcgtt attcacagaa tatccagggg atgacaggaa
    22501 ctcccatggt ggctgctagt ccggagagac atgatggtgg tttacaggct aacccagtag
    22561 aggtgcaaag ttaccagccg ccctggaaag tactgagtga cttcgccttg caaagcgaca
    22621 tagatcagcc tgcttttcag caactggtaa gtgtctggct cccaaatggg aaaagactgg
    22681 atccctaaca aaaaaaggag actcgtttta actgttaggc attgaaaggt cccctggggt
    22741 gagggagggt accgggatta gagagaaagc aggatgtggt ggtgaagagg agggaggcag
    22801 atggaaggaa aggagatctc ttgtgaatct ttgtttaaaa ctgagatgcc acaatttaac
    22861 tatgtagcct aagtgtgtag tgtgatcttc ctacaaagga ggcatatctt atgggttgag
    22921 cttctcagtg cctaagtagt tctgtgtaca gaggtacaga cagtggcaat gtcacatatt
    22981 ttaggctgtt ctaaattaca aggacttcag agaccaccgt ggcatttgga tcagcaatca
    23041 gtaagttctt tgctttacct gtgtcttgtg actgattcaa gtaaaaatga ctgggatgca
    23101 cgcccagtgt gatacccagt tagaatgcag ttgtgcataa gcagcaggga gctgtctgaa
    23161 cacaattctc ttctatgtca accacgagga tagttattta aata
//