LOCUS       KO-first_condition_ready_40628_MGI:101784        21615 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Nes targeting project(s): 40628
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_P
COMMENT     design_id: 115801
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            8251..9146
                     /db_xref="ENSEMBL:ENSMUSE00000333301"
                     /rank="1"
                     /note="ENSMUSE00000333301"
     misc_feature    4215..9792
                     /note="5 arm"
     misc_feature    9820..9844
                     /note="B1 Gateway"
     misc_feature    9857..9904
                     /note="Frt"
     intron          9905..10779
                     /note="mouse En2 intron"
     misc_feature    10777..10779
                     /note="Splice Acceptor"
     exon            10780..10781
                     /note="mouse En2 exon"
     misc_feature    10993..11554
                     /note="ECMV IRES"
     gene            11565..14611
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14646..14901
                     /note="SV40 polyadenylation site"
     misc_feature    14905..14938
                     /note="loxP"
     promoter        14970..15477
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            15564..16355
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16553..16796
                     /note="SV40 polyadenylation site"
     misc_feature    16813..16860
                     /note="Frt"
     misc_feature    16867..16900
                     /note="loxP"
     misc_feature    10794..10871
                     /note="FCHK std_gpr"
     misc_feature    16916..16936
                     /note="B2 Gateway"
     misc_feature    9793..16966
                     /note="Synthetic Cassette"
     exon            17303..17427
                     /db_xref="ENSEMBL:ENSMUSE00000175774"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000175774"
     misc_feature    16967..17558
                     /note="Critical Region"
     misc_feature    17582..17615
                     /note="Downstream LoxP"
     misc_feature    17559..17638
                     /note="synthetic loxP region"
     exon            19171..19244
                     /db_xref="ENSEMBL:ENSMUSE00000175771"
                     /rank="3"
                     /note="ENSMUSE00000175771"
     exon            19790..21615
                     /db_xref="ENSEMBL:ENSMUSE00000567238"
                     /rank="4"
                     /note="ENSMUSE00000567238 5' fragment"
     misc_feature    17639..21615
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggagctgaa gtctcacaga ttcctcaaag ccaagaatac agctcaaaga
     4261 ttacttgtcc agtattcatg gtgtcctggg gtccatctgt aaccaatgca gcccccaaag
     4321 gttgagagag ggttccctgt aggtataaat tttttgcaac ctacttttaa taagggttct
     4381 atccctacca cccagcaaag gtagtagaag agaaaagtta ttaggctatg gtggaagtgg
     4441 acctgttcag caatagttct ttgggaacaa gctcgatttg ctttgacagc agttcattcc
     4501 catagcaaac accaaatacg actcagcagc tgcagaccag tcctctaggc aggcagacac
     4561 catgcatgaa ccagcagcta cagtccagtt ctctcagcaa gcagacacca ggcagctgta
     4621 gttcaatcct gaagaaactg ccaggcttac cagcctgcct gaggtgaggc tgcagaagca
     4681 gcaagctgcc tcgggaacct tgagagcagt gcttgggtga gtttctctcg gtggcagcat
     4741 tatcacaagg tgagcccaac aacactatat atgatgaatc aatacatgtg tgcctttagc
     4801 gaagaatatc aaggtggagc aaacaaaact cagtgctcgt ctcttattgc ctgtggggtc
     4861 atatttatac tccttcatca agagtcctct catgtgtttg ctatatccaa acatcctctc
     4921 acctgtgtct gcttcaggaa aacagcctct cacgtgtttg ctttagtaag acatgctttc
     4981 acctgtgtgc tccagtcaaa catttgacat cactaactta ttcagggcta ttgagatgga
     5041 aggggcgaat tcagagccac agccctgtgc aaggcaccgc agtctctcta gaacacgatc
     5101 agtgctcaag gaactctgct gagaaggaca gagaagggcc acttatcctc tttccactgg
     5161 agacctcagt gcccgaggaa catttgcatg atccacagta gcaagtatgt aacaggcata
     5221 caagccaaat actgataatc ataaagacat ttatgttaga gcaaatctct ggtatacaga
     5281 tgatttttcc atttttaaaa gataaaagaa ggggtggggg agtctgggga gaacgttcag
     5341 aagtggcacc tcacgagggg aaggaaaaga gggaagaaca ggtcaccagc cttggacctc
     5401 aggtgactga aggagagaat gtatttgttg cctgtcagac ctttgcgtcc ttcagtgaca
     5461 cccttgtcca tgttactgat ctttctggca aggaaaccgt ctgccgtgtg actggcagga
     5521 tgaaggtaaa ggctgcctga gatgagtcct ctccgtatgc agccatgttg gctgcccagg
     5581 atgttgccca gaggtgcaag gggctgggca tcaccaccct gtgtatcaaa ctccgggcca
     5641 caggaggaaa caggaccaag accccaggac ctggagccca gtcagccctc agagctctgg
     5701 gatgaggatt gggcagatag aagttgtcat ccccatcccc tctgacagca ctggaaggaa
     5761 gagggtcatc atggttgtca tctgtgaaca ggacttctca aattattttc tgttaatgaa
     5821 ttgctttgtg taagctaaaa aaaaaaaaaa agatgaaagg agatttccct gcgaatggga
     5881 aggattgctt gcttattgtt ttttcgagac aggtttctct gtgtagccct ggctgtcctg
     5941 gaactcactt tgtagaccag gctggcctcg aactcagaga tccacctgcc tccacctgcc
     6001 tctgcctccc aagtgctggg attaaaggca tgcgccacca ctgcctggcc tgcttgttta
     6061 tgcttatgta gagctgaatc ttgcctgaac atttggtact tcaggtccat gatggaacac
     6121 cttcaacatt ttcagaattg atccatactt taatttaaaa aaatagttta ttgttatttg
     6181 tgtgtgtgtg tgtgtgtgtg tgtgtgcatg ggcgtgtata tctgtgtgtg gatgcatgca
     6241 gaggtcagag gctttgatgt ccctggagct gcatgtgggt gctgggaatg gtacttgagt
     6301 cctctggaag agcagcaagt gcttttgact actgagcgtc tgtctctcca gcccagatat
     6361 cctgatttcg taaccatcag tgctgagaat gccaatctgt atgaacgatt ctgcccatag
     6421 aagtatgttt aggaccattt cagacatttt tggaaatttt gtcctaatcc caagtcaaaa
     6481 ttcactgaac gatttattta tttattatat ataagtacac tgtagctgtc ttcagacaca
     6541 ccagaagagg gcatcagatc tcattgcaga tggttgtgag ccaccgtgta gttgcttgga
     6601 attgaactga ggacctctgg aagggtagtc agtgctctta attactgagc agtctttcca
     6661 gccccctatt ttttttttct tttaaggcag gatctgacta tgtaatcctg ggtggcccag
     6721 aactcatttg ctatgtagag gctggcctca aactcagaga ttggctttat tctttctctt
     6781 gcgtgctggg gttaaaggga ttaaactggc ctcactgaac aaagtttgat gaaactcatg
     6841 tcattgactc aaacagctat tttaaagaat ttagatttat tattgttctt cttattattg
     6901 gtgtgtgtgt gtgtgtgtgt gtgtacaggt gcaccatgcc acagtatgcc atgtgggaac
     6961 cagtctctcc tcagctcact atgcgggtcc tgaacatcac agtagggagc atggcaacag
     7021 gcaggcggtt atggcactgg aacagcagct gagagctcag gtctcaatgt acaagcacaa
     7081 gcctgccccc agtgacacac ctcctccaac aaggccattc ctcccaatcc tttttttttt
     7141 tttttttttt tttttggatt tttgagacag ggtttctctg tgtagctctg gctgtcctgg
     7201 aactcactct gtagaccagg ctggtctcga actcagaaat ccgcctgcct ttgcctccca
     7261 agtgctggga ttaaagacat gtgccaccac tgcccggccc caatcttttt aaacaggtcc
     7321 tccacctggg gagcaagcat tcaaatattt gagcctgaaa tattggtccc ttgtagaaaa
     7381 tctacagctt catttctttc ttcgccaaac ttcatgctta gccaccaggg ggctctcttg
     7441 agagccgcgt aacttcttca ctaaatgaca agagggatcg aacttcaaag gagaaaaact
     7501 ttcccttgct agtctccttt tcctggtgcg gggtagggta gggtaggacg tccctctgcg
     7561 ggtacgaact gggcattgtg tctgctaaca ttaactgggc aggggtactg gcacaggcat
     7621 ttaatcccag cactggggaa gctatggcag gagcgtccct gagacctgcc tgatctacat
     7681 agccatacca accatctgaa gaaaaggggt ggtggtggtg tttactaccc aggctggtgt
     7741 caatcctact tcagcctccg gagcactggc agacttccct cagtaccagt gggctcctct
     7801 ttcttcgggc agtgtttctt cgcaatgttg cctctcttgg gggtgagtat ttactgttat
     7861 acacaaaaca ctgcaccgtc cgtttttcca acagttcacg aggtttccaa caccaagcca
     7921 ttttataaat gaggagctga gtcctagagg atttgagtaa gagtgtagag aaaggaggtc
     7981 ctggggccca gttctgtgca tcttagggtg ttctgggctg tctggctgta tctcaagcct
     8041 ctttcggaaa atcacccgca ccggacggga tccccgccag ggcgaggcta caatttgatt
     8101 cttctctgct gagctgggat gatgcaggga cccgggctgt gtgttgcact gaactctaaa
     8161 gggttaaggc ctagggaccg ccccttttcc gcccggccgg cgggagtatg aataccctcg
     8221 cttcagctcg ctgctggaat cctccgcttc cgctgggtca ctgtcgccgc tactcccttt
     8281 ctccccctta aaagctccaa gggccactcc cttctctagt gctccacgtc cgcttgccct
     8341 cggggggcca gaccagcgac atggagggtt gcgtcgggga agaatctttt cagatgtggg
     8401 agctcaatcg acgcctggag gcctacctga cccgggtcaa gacgctggag gagcagaacc
     8461 agctgctcag tgccgagctt gggggactcc gggcgcagtc cggggacgcc tcctggcgag
     8521 cccgagccga cgacgagctg gcagccctgc gggtcctcgt cgatcagcgc tggcgggaga
     8581 agcacgaggc tgaggtgcag cgcgacaacc ttgccgaaga gctggagagc gtggcgggcc
     8641 ggtgccagca ggtgcggctc gcccgggagc ggaccatcga ggaggcggcc tgcagccggc
     8701 gcgcgctcga ggcggagaag aatgcgcggg gctggctgag cacccaggct gccgagctgg
     8761 agcgcgagtt agaggctctg cgagcgtcgc acgaggagga gcgcgcgcac ctgaacgccc
     8821 aggccgcctg tacgccgcgc cgaccccccg cgccggccca cgcatccccc atccgggccc
     8881 ctgaagtcga ggagctggcc aggcgcctag gcgaagtgtg gcgcggggcg gtgcgtgact
     8941 accaggagcg cgtggctcac atggagagct cgctgggcca ggcccgcgag cgtctgggcc
     9001 aagccgtgcg gggcgctcgg gagagtcgct tagaggtgca gcagctgcag gctgatcgcg
     9061 acagcctcca ggagcgcaga gaggcgctgg aacagagatt ggaaggccgc tggcaggacc
     9121 ggctgcaggc cactgaaaag ttccaggtga ggagggtcac caacacgccc accctgtccc
     9181 tggtaaaacc aagccctagg aggtacttag gatggcaaga gaccttcagg aaaggggtgg
     9241 gggcaaggac aactgtattc aggagtcctt gctcagagca agcacaagtc ctagacttcc
     9301 ctgtccagac aggcaggatg gatggaattc tgagacctcc tagcacagcc cccttgaaag
     9361 gcccgcaggc caatacccac tgtagctgct cctcactctt tgaatggcaa cccctctcta
     9421 gggtgactcc ctgtgccctt cactatgcag ttgtcaatca gaaggtgccc aacacatgct
     9481 ctctccccac cagaaggttc ctagcaacca tagtgaagga aggcagtgtg agccaccgca
     9541 gaccaccagc ccctctctgt gtcaaggctt ctcagctccc tgaagcgcat tccaaaggcc
     9601 tggggcaccc tccccctcat attgcacaga tgtgaccaac agctgagaaa acagcctctt
     9661 gcctggccca gacagaagtc ccgacgtgac cacctgccca ctcttcttgg gggagaagga
     9721 ttccagaaca agtgggaagt ggaggaaccc agcctgctat gaggagccgg gggcagtggt
     9781 ggcccagctg ctaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca
     9841 ggctggcgcc ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     9901 cttcgaaccc tttcccacac caccctccac acttgcccca aacactgcca actatgtagg
     9961 aggaaggggt tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt
    10021 tatgttcttg cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg
    10081 gggtagaagg tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag
    10141 ccaggtccct ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct
    10201 ggggtaccct attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg
    10261 gctcctcctg ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa
    10321 gggggacggg attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc
    10381 tctcacctgt ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa
    10441 caaatgcaaa cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg
    10501 gatcccctag tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac
    10561 acacacacac cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac
    10621 cacccccaat gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag
    10681 tatctgcaac ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc
    10741 aaccagtaac ctctgccctt tctcctccat gacaaccagg tcccaggtcc cgaaaaccaa
    10801 agaagaagaa ccctaacaaa gaggacaagc ggcctcgcac agccttcact gctgagcagc
    10861 tccagaggct caaggctgag tttcagacca acaggtacct gacagagcag cggcgccaga
    10921 gtctggcaca ggagctcggt acccggaaga tctggactct agagaattcc gcccctctcc
    10981 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
    11041 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
    11101 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
    11161 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
    11221 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
    11281 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
    11341 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
    11401 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
    11461 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
    11521 tttcctttga aaaacacgat gataagcttg ccacaaccat ggaagatccc gtcgttttac
    11581 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11641 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11701 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
    11761 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
    11821 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
    11881 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
    11941 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
    12001 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
    12061 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga
    12121 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
    12181 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
    12241 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
    12301 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
    12361 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
    12421 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
    12481 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
    12541 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
    12601 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
    12661 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
    12721 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
    12781 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
    12841 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
    12901 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
    12961 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
    13021 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
    13081 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
    13141 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
    13201 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
    13261 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
    13321 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
    13381 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
    13441 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
    13501 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
    13561 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
    13621 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
    13681 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
    13741 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
    13801 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
    13861 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
    13921 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
    13981 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
    14041 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
    14101 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
    14161 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
    14221 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
    14281 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
    14341 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
    14401 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
    14461 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
    14521 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
    14581 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggggggatc
    14641 taagctctag ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg
    14701 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    14761 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    14821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    14881 tatcttatca tgtctggatc cggaataact tcgtatagca tacattatac gaagttatgt
    14941 ttctagcttg gctgcaggtc gtcgaaattc taccgggtag gggaggcgct tttcccaagg
    15001 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
    15061 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
    15121 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
    15181 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
    15241 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
    15301 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
    15361 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
    15421 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
    15481 agcctgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    15541 ggaactaaac catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
    15601 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    15661 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    15721 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
    15781 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    15841 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    15901 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    15961 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    16021 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    16081 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    16141 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    16201 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    16261 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    16321 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
    16381 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
    16441 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    16501 tctcatgctg gagttcttcg cccacccccc ggatctaagc tctagataag taatgatcat
    16561 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    16621 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    16681 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    16741 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgggg
    16801 gtaccgcgtc gagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
    16861 gtcgagataa cttcgtatag catacattat acgaagttat gtcgagatat ctagacccag
    16921 ctttcttgta caaagtggtt gatatctcta tagtcgcagt aggcgggcga ctcttgataa
    16981 attgcttttt ccttctgttt ccactttgcc tcctcttccg ctgtgtaacc catctcctct
    17041 cctttatccc atctcctccc gtgggagcta ggttctcaat agcatgtgtt accatttaac
    17101 cagcatccta agaagaagcc tggtctatct aggagaggcc tggagtgttt cctgtgcttg
    17161 tccgggggtc cagcacagtg ctccagacag ggagaacgca ggtagaggaa caggactctg
    17221 ccctacaaag atgtgagcag tgaagagagt catggcccag agcagaggcc cctcccactc
    17281 actctgccgg cttgctttgc agctggctgt ggaagccctg gagcaggaga agcagggtct
    17341 acagagtcag atcgctcaga tcctggaagg tgggcagcaa ctggcacacc tcaagatgtc
    17401 ccttagtctg gaagtggcta catacaggta cacagtactg actgtctcct tggctgaagg
    17461 gccccgaaac ttgaccctgt aaaactgttc tatttacgtg tatttgtgta taggccccat
    17521 gtgtccaggt gtcttaagag cccagaagac actgggtcga gatggcgcaa cgcaattaat
    17581 gataacttcg tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcatg
    17641 tgagcagctc cacactggtg ctgtgagcca cacccctgtt ctctgcaagg gcagcaagca
    17701 ctcttaacca ctgagccatc tgtccagccc tcagatccac ttaaaacttt ttattacatg
    17761 tatttatgta atgtttttgt ctgtgatgga ggcttatgag tcccagagat caaagaggtt
    17821 gtctggcttg gcagcttggt aggcataaac ttgagcccac ctgctcccag gttcgggact
    17881 agaagagatg gttatgctta aagagacaac tcagtcttca gggaggagac cagagggtac
    17941 agtcacctgc tctcgagttc tttgaaaggc aggcaatctc cagtgtctat gccagatccc
    18001 agggacacct gggcggtctc aggaaagaga ccttgcagga ttgcctggtc gtgctcaccg
    18061 gtcccctagc ccgggcctca gctgtgtctg gggtaagagc tgggcgccta gccttctgcc
    18121 ttcctttcaa gccctcgtgt cactggccct gcccagctcc tgttgcccct ttcttggctg
    18181 cctcaggaag gctgagtgga acagctgtgg gtagtttgtg tccagcctct gtctgtcaga
    18241 agaggtcccg gctggagtcc cactgttgag ggctgaggat atcccgccag agtatggggc
    18301 caggacagga tgtcgccctt ctccagaatc cactggcaaa gaggcaagac cagagacaga
    18361 tctaggagag gagctggagg gtagagtcca gagaagggaa gccagtgagt gtctaggaag
    18421 gactgaatgc acaaggcagg acactatgag gacggaggaa aggctatttt gagaaccaga
    18481 tgtgctcaga ggccatgaat ggaaacaagg gactagctcc gaatcccttg tgaactgatt
    18541 cccctcatct cctcccatca gctgtgaacc taaagacctc tggcctgaag gaggatttgt
    18601 gatcctaaga tgagggcctt cgccccagtc agtcttctga ggcgagggga agggtccagg
    18661 cagctctgag gaatgtaagc actggtgttt gcggtctgaa aaggatttgg agaaggagag
    18721 ctgaattcat ttgcttttgt ctgtcaccag ctctgggggc ctaggagaga gccatcccat
    18781 gggaacagcc tgagaattcc cacttcccct gaggagcccc tccttctcag gccctccaga
    18841 tggtagtgtg gacaaaaggc aataattagc atgagaatcg gcctccctct ccgaggatga
    18901 ggtcatcggc cttggccttg ggtggggagg cggagactga tctgaggagt ctgatcgaag
    18961 tgttagcaat tcacttggcc ctgcttccga atgtgggaat ctgcgtgtgg ggtctccctg
    19021 agtctcaaat atgggttcac caagtatata tctgtgggta tatgactgtg tggctttcat
    19081 acgacaatgg tcacaataaa gagtgatcct gcagtggcag gacatgcaac ctcagctggg
    19141 cctgacccca tcgcctcccc tcccccccag gactctgctg gaggctgaga actctcgctt
    19201 gcagacacct ggaagaagtt cccaggcttc tcttggcttt cctggtaaga ggcggagcca
    19261 actgctcctg gacgctctcc ttccacatct tccctaagca gcatcagtcc aagtcttcac
    19321 tccctggtcc ccacagtact gatgtcccca gtctaagcag tgggggtcgg ataaaatggc
    19381 tgaaatgagg gctgctctgg gtgaagtcag gacatactta cagtgtttct tttctttttt
    19441 aagatttatt tattttatgt atatgagtat actatagctg tacagatggt tgtgaacctt
    19501 cgtgtgattg ttgggaattg aatttaggac ctctgctggc tccagtctgc cctgctcgct
    19561 cagtccctat gcactctggc ccaaagattt atttattatt ataaataagt acactgttgc
    19621 tgtcttcaga catactagaa gaaggcgtga gatctcatta cgggtggttg tgagccacca
    19681 tatggttgct gggatttgaa ctcaggacct tcggaagaac agtcagtgcc cttacccgct
    19741 gagccatctc gccagcccac ttacagtgtt tcttactctg cttttccaga ccccaagctg
    19801 aagctgcatt tccttgggat accagaggac cagcacctgg gatctgtgct ccctgtcctc
    19861 agcccaacat ccttctcttc ccccttgcct aatacccttg agactcctgt gacagccttt
    19921 ctgaagacac aggaattcct taaggccaga acccccacct tggccagcac tcccatccca
    19981 cctatgtctg aggctcccta tcctaaaaat gcagaggtca gagcccagga tgtcccccat
    20041 tccctgctcc agggtgggag gcaacaggct ccagagcctc tttgggctga ggccacagtg
    20101 cccagttcta ctggtgtcct cccagagctg gaggagcctg ggggcgagca gccggaccac
    20161 ttccctgatg atccaacctc cttagcccca cccctcaacc ctcaccactc tattttagag
    20221 gctaaagata gagaatccag tgagtctaga gtttctagca tattccagga agaagaaggg
    20281 caaatctggg aacttgtaaa gaaagaagca gccacagagg taaaagtaga aaacagctta
    20341 gcacaggaaa tacaagaaag tggtctggac acagaagaaa tccaggattc ccagggacct
    20401 ttgcagatgg aaaccctgga ggctctagga gatgagccac tgatgtctct gaaaacccag
    20461 aaccatgaga ccccaggaaa ggagaattgc aattcatcta tagaagagaa ctcggggaca
    20521 gtaaaaagcc cagaaaaaga aaaacaaaca ccactgaagt ctttagaaga aaagaatgta
    20581 gaggcagaga aaactctaga aaatggggtt cttgaactat ctaaaccttt aggagaagaa
    20641 gaaccaagaa tggaggatca agaattaatg tctcctgaac acacactaga gacagtttca
    20701 tttctaggaa aggaaaatca ggaagtagtg aggtcttcag aagaacagaa cttagaatca
    20761 ttgataactt ttaaagagga gagccaatac ccactgggag gtccagaagc cgaggaccag
    20821 atgcttgaaa gactggtaga gaaagaggat cagaggttcc caaggtctcc agaagaagac
    20881 cagcaggcgt ttagacctct ggagaaagag aatcaggagc cactaagatt tgaagaagca
    20941 gaggaccagg tgcttgagag actgatagaa aaggaaaggc aggagtccct gaagtctcca
    21001 gaagaagagg accagcaggc atttagactt ctggagaaag agaatcaaga accactaagg
    21061 tttgaagacg cagaggacca ggtgcttgag agactgatag aaaaggaaag acaggagtcc
    21121 ctgaagtctc cagaagaaga ggaccagcag gcatttagac ttctggagaa agagaatcaa
    21181 gaaccactaa ggtttgaaga agcagaggac caggtgcttg agagactggt agaaaaggaa
    21241 agtcaggagt ccctgaagtc tccagaagag gaggaccaga ggactgggaa gcctctagaa
    21301 aaagaaaatc aggaatctct gaggtctctt gatgaaaacc aggagacaat tgtactgcta
    21361 gaaagcaaga accagaggcc actgagatct ctagaagtag aagaggagga gcagagaatt
    21421 gtgaaacctc tagaaaaagt gagccaggtc tccctcgaat ctctcgaaaa agagaatgtg
    21481 cagtcaccaa ggtatctgga agaagatgac cacatgatta agagcctgct agaagacaag
    21541 actcatgaga tcctgggatc tcttgaagat agaaatgggg agaactttat accacctgaa
    21601 aatgagaccc agggt
//