LOCUS       KO-first_condition_ready_70691_MGI:101784        22782 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Nes targeting project(s): 70691
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 115801
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            9470..10365
                     /db_xref="ENSEMBL:ENSMUSE00000333301"
                     /rank="1"
                     /note="ENSMUSE00000333301"
     misc_feature    5434..11011
                     /note="5 arm"
     misc_feature    11039..11063
                     /note="B1 Gateway"
     misc_feature    11076..11123
                     /note="Frt"
     intron          11124..11998
                     /note="mouse En2 intron"
     misc_feature    11996..11998
                     /note="Splice Acceptor"
     exon            11999..12171
                     /note="mouse En2 exon"
     misc_feature    12183..12776
                     /note="ECMV IRES"
     gene            12777..15837
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15876..16119
                     /note="SV40 polyadenylation site"
     misc_feature    16123..16156
                     /note="loxP"
     promoter        16183..16702
                     /note="human beta actin promoter"
     gene            16728..17522
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17722..17963
                     /note="SV40 polyadenylation site"
     misc_feature    17980..18027
                     /note="Frt"
     misc_feature    18034..18067
                     /note="loxP"
     misc_feature    12013..12090
                     /note="FCHK std_gpr"
     misc_feature    18083..18103
                     /note="B2 Gateway"
     misc_feature    11012..18133
                     /note="Synthetic Cassette"
     exon            18470..18594
                     /db_xref="ENSEMBL:ENSMUSE00000175774"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000175774"
     misc_feature    18134..18725
                     /note="Critical Region"
     misc_feature    18749..18782
                     /note="Downstream LoxP"
     misc_feature    18726..18805
                     /note="synthetic loxP region"
     exon            20338..20411
                     /db_xref="ENSEMBL:ENSMUSE00000175771"
                     /rank="3"
                     /note="ENSMUSE00000175771"
     exon            20957..22782
                     /db_xref="ENSEMBL:ENSMUSE00000567238"
                     /rank="4"
                     /note="ENSMUSE00000567238 5' fragment"
     misc_feature    18806..22782
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggagctgaag tctcacagat tcctcaaagc
     5461 caagaataca gctcaaagat tacttgtcca gtattcatgg tgtcctgggg tccatctgta
     5521 accaatgcag cccccaaagg ttgagagagg gttccctgta ggtataaatt ttttgcaacc
     5581 tacttttaat aagggttcta tccctaccac ccagcaaagg tagtagaaga gaaaagttat
     5641 taggctatgg tggaagtgga cctgttcagc aatagttctt tgggaacaag ctcgatttgc
     5701 tttgacagca gttcattccc atagcaaaca ccaaatacga ctcagcagct gcagaccagt
     5761 cctctaggca ggcagacacc atgcatgaac cagcagctac agtccagttc tctcagcaag
     5821 cagacaccag gcagctgtag ttcaatcctg aagaaactgc caggcttacc agcctgcctg
     5881 aggtgaggct gcagaagcag caagctgcct cgggaacctt gagagcagtg cttgggtgag
     5941 tttctctcgg tggcagcatt atcacaaggt gagcccaaca acactatata tgatgaatca
     6001 atacatgtgt gcctttagcg aagaatatca aggtggagca aacaaaactc agtgctcgtc
     6061 tcttattgcc tgtggggtca tatttatact ccttcatcaa gagtcctctc atgtgtttgc
     6121 tatatccaaa catcctctca cctgtgtctg cttcaggaaa acagcctctc acgtgtttgc
     6181 tttagtaaga catgctttca cctgtgtgct ccagtcaaac atttgacatc actaacttat
     6241 tcagggctat tgagatggaa ggggcgaatt cagagccaca gccctgtgca aggcaccgca
     6301 gtctctctag aacacgatca gtgctcaagg aactctgctg agaaggacag agaagggcca
     6361 cttatcctct ttccactgga gacctcagtg cccgaggaac atttgcatga tccacagtag
     6421 caagtatgta acaggcatac aagccaaata ctgataatca taaagacatt tatgttagag
     6481 caaatctctg gtatacagat gatttttcca tttttaaaag ataaaagaag gggtggggga
     6541 gtctggggag aacgttcaga agtggcacct cacgagggga aggaaaagag ggaagaacag
     6601 gtcaccagcc ttggacctca ggtgactgaa ggagagaatg tatttgttgc ctgtcagacc
     6661 tttgcgtcct tcagtgacac ccttgtccat gttactgatc tttctggcaa ggaaaccgtc
     6721 tgccgtgtga ctggcaggat gaaggtaaag gctgcctgag atgagtcctc tccgtatgca
     6781 gccatgttgg ctgcccagga tgttgcccag aggtgcaagg ggctgggcat caccaccctg
     6841 tgtatcaaac tccgggccac aggaggaaac aggaccaaga ccccaggacc tggagcccag
     6901 tcagccctca gagctctggg atgaggattg ggcagataga agttgtcatc cccatcccct
     6961 ctgacagcac tggaaggaag agggtcatca tggttgtcat ctgtgaacag gacttctcaa
     7021 attattttct gttaatgaat tgctttgtgt aagctaaaaa aaaaaaaaaa gatgaaagga
     7081 gatttccctg cgaatgggaa ggattgcttg cttattgttt tttcgagaca ggtttctctg
     7141 tgtagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagagat
     7201 ccacctgcct ccacctgcct ctgcctccca agtgctggga ttaaaggcat gcgccaccac
     7261 tgcctggcct gcttgtttat gcttatgtag agctgaatct tgcctgaaca tttggtactt
     7321 caggtccatg atggaacacc ttcaacattt tcagaattga tccatacttt aatttaaaaa
     7381 aatagtttat tgttatttgt gtgtgtgtgt gtgtgtgtgt gtgtgcatgg gcgtgtatat
     7441 ctgtgtgtgg atgcatgcag aggtcagagg ctttgatgtc cctggagctg catgtgggtg
     7501 ctgggaatgg tacttgagtc ctctggaaga gcagcaagtg cttttgacta ctgagcgtct
     7561 gtctctccag cccagatatc ctgatttcgt aaccatcagt gctgagaatg ccaatctgta
     7621 tgaacgattc tgcccataga agtatgttta ggaccatttc agacattttt ggaaattttg
     7681 tcctaatccc aagtcaaaat tcactgaacg atttatttat ttattatata taagtacact
     7741 gtagctgtct tcagacacac cagaagaggg catcagatct cattgcagat ggttgtgagc
     7801 caccgtgtag ttgcttggaa ttgaactgag gacctctgga agggtagtca gtgctcttaa
     7861 ttactgagca gtctttccag ccccctattt tttttttctt ttaaggcagg atctgactat
     7921 gtaatcctgg gtggcccaga actcatttgc tatgtagagg ctggcctcaa actcagagat
     7981 tggctttatt ctttctcttg cgtgctgggg ttaaagggat taaactggcc tcactgaaca
     8041 aagtttgatg aaactcatgt cattgactca aacagctatt ttaaagaatt tagatttatt
     8101 attgttcttc ttattattgg tgtgtgtgtg tgtgtgtgtg tgtacaggtg caccatgcca
     8161 cagtatgcca tgtgggaacc agtctctcct cagctcacta tgcgggtcct gaacatcaca
     8221 gtagggagca tggcaacagg caggcggtta tggcactgga acagcagctg agagctcagg
     8281 tctcaatgta caagcacaag cctgccccca gtgacacacc tcctccaaca aggccattcc
     8341 tcccaatcct tttttttttt tttttttttt ttttggattt ttgagacagg gtttctctgt
     8401 gtagctctgg ctgtcctgga actcactctg tagaccaggc tggtctcgaa ctcagaaatc
     8461 cgcctgcctt tgcctcccaa gtgctgggat taaagacatg tgccaccact gcccggcccc
     8521 aatcttttta aacaggtcct ccacctgggg agcaagcatt caaatatttg agcctgaaat
     8581 attggtccct tgtagaaaat ctacagcttc atttctttct tcgccaaact tcatgcttag
     8641 ccaccagggg gctctcttga gagccgcgta acttcttcac taaatgacaa gagggatcga
     8701 acttcaaagg agaaaaactt tcccttgcta gtctcctttt cctggtgcgg ggtagggtag
     8761 ggtaggacgt ccctctgcgg gtacgaactg ggcattgtgt ctgctaacat taactgggca
     8821 ggggtactgg cacaggcatt taatcccagc actggggaag ctatggcagg agcgtccctg
     8881 agacctgcct gatctacata gccataccaa ccatctgaag aaaaggggtg gtggtggtgt
     8941 ttactaccca ggctggtgtc aatcctactt cagcctccgg agcactggca gacttccctc
     9001 agtaccagtg ggctcctctt tcttcgggca gtgtttcttc gcaatgttgc ctctcttggg
     9061 ggtgagtatt tactgttata cacaaaacac tgcaccgtcc gtttttccaa cagttcacga
     9121 ggtttccaac accaagccat tttataaatg aggagctgag tcctagagga tttgagtaag
     9181 agtgtagaga aaggaggtcc tggggcccag ttctgtgcat cttagggtgt tctgggctgt
     9241 ctggctgtat ctcaagcctc tttcggaaaa tcacccgcac cggacgggat ccccgccagg
     9301 gcgaggctac aatttgattc ttctctgctg agctgggatg atgcagggac ccgggctgtg
     9361 tgttgcactg aactctaaag ggttaaggcc tagggaccgc cccttttccg cccggccggc
     9421 gggagtatga ataccctcgc ttcagctcgc tgctggaatc ctccgcttcc gctgggtcac
     9481 tgtcgccgct actccctttc tcccccttaa aagctccaag ggccactccc ttctctagtg
     9541 ctccacgtcc gcttgccctc ggggggccag accagcgaca tggagggttg cgtcggggaa
     9601 gaatcttttc agatgtggga gctcaatcga cgcctggagg cctacctgac ccgggtcaag
     9661 acgctggagg agcagaacca gctgctcagt gccgagcttg ggggactccg ggcgcagtcc
     9721 ggggacgcct cctggcgagc ccgagccgac gacgagctgg cagccctgcg ggtcctcgtc
     9781 gatcagcgct ggcgggagaa gcacgaggct gaggtgcagc gcgacaacct tgccgaagag
     9841 ctggagagcg tggcgggccg gtgccagcag gtgcggctcg cccgggagcg gaccatcgag
     9901 gaggcggcct gcagccggcg cgcgctcgag gcggagaaga atgcgcgggg ctggctgagc
     9961 acccaggctg ccgagctgga gcgcgagtta gaggctctgc gagcgtcgca cgaggaggag
    10021 cgcgcgcacc tgaacgccca ggccgcctgt acgccgcgcc gaccccccgc gccggcccac
    10081 gcatccccca tccgggcccc tgaagtcgag gagctggcca ggcgcctagg cgaagtgtgg
    10141 cgcggggcgg tgcgtgacta ccaggagcgc gtggctcaca tggagagctc gctgggccag
    10201 gcccgcgagc gtctgggcca agccgtgcgg ggcgctcggg agagtcgctt agaggtgcag
    10261 cagctgcagg ctgatcgcga cagcctccag gagcgcagag aggcgctgga acagagattg
    10321 gaaggccgct ggcaggaccg gctgcaggcc actgaaaagt tccaggtgag gagggtcacc
    10381 aacacgccca ccctgtccct ggtaaaacca agccctagga ggtacttagg atggcaagag
    10441 accttcagga aaggggtggg ggcaaggaca actgtattca ggagtccttg ctcagagcaa
    10501 gcacaagtcc tagacttccc tgtccagaca ggcaggatgg atggaattct gagacctcct
    10561 agcacagccc ccttgaaagg cccgcaggcc aatacccact gtagctgctc ctcactcttt
    10621 gaatggcaac ccctctctag ggtgactccc tgtgcccttc actatgcagt tgtcaatcag
    10681 aaggtgccca acacatgctc tctccccacc agaaggttcc tagcaaccat agtgaaggaa
    10741 ggcagtgtga gccaccgcag accaccagcc cctctctgtg tcaaggcttc tcagctccct
    10801 gaagcgcatt ccaaaggcct ggggcaccct ccccctcata ttgcacagat gtgaccaaca
    10861 gctgagaaaa cagcctcttg cctggcccag acagaagtcc cgacgtgacc acctgcccac
    10921 tcttcttggg ggagaaggat tccagaacaa gtgggaagtg gaggaaccca gcctgctatg
    10981 aggagccggg ggcagtggtg gcccagctgc taaggcgcat aacgatacca cgatatcaac
    11041 aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg aagttcctat
    11101 tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca cttgccccaa
    11161 acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg ttgtggggaa
    11221 gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat
    11281 gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag gtcagccccc
    11341 cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa ggggagatgc
    11401 cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac aaacttggcc
    11461 tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga tccccttgta
    11521 gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc tactcttttc
    11581 ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga tagacttgga
    11641 aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc tgggcattcc
    11701 ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct acatctgcca
    11761 atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg gcacatgtcc
    11821 agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt gggcgggtca
    11881 tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga
    11941 taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg acaaccaggt
    12001 cccaggtccc gaaaaccaaa gaagaagaac cctaacaaag aggacaagcg gcctcgcaca
    12061 gccttcactg ctgagcagct ccagaggctc aaggctgagt ttcagaccaa caggtacctg
    12121 acagagcagc ggcgccagag tctggcacag gagctcggta cccggaagat ctggactcta
    12181 gagaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
    12241 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    12301 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    12361 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    12421 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    12481 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    12541 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    12601 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
    12661 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
    12721 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaaccatgg
    12781 aagatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    12841 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    12901 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
    12961 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
    13021 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
    13081 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
    13141 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
    13201 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
    13261 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
    13321 tgatggtgct gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
    13381 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
    13441 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
    13501 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
    13561 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
    13621 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
    13681 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
    13741 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
    13801 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
    13861 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
    13921 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
    13981 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
    14041 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
    14101 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
    14161 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
    14221 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
    14281 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
    14341 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
    14401 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
    14461 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
    14521 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
    14581 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
    14641 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
    14701 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
    14761 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
    14821 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
    14881 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
    14941 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
    15001 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
    15061 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
    15121 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
    15181 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
    15241 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
    15301 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
    15361 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
    15421 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
    15481 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
    15541 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
    15601 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
    15661 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
    15721 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
    15781 aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat
    15841 aaccgggcag gggggatcta agctctagat aagtaatgat cataatcagc catatcacat
    15901 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    15961 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    16021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    16081 tgtccaaact catcaatgta tcttatcatg tctggatccg gaataacttc gtatagcata
    16141 cattatacga agttatgttt aaacggcgcg ccccggaatt cgccttctgc aggagcgtac
    16201 agaacccagg gccctggcac ccgtgcagac cctggcccac cccacctggg cgctcagtgc
    16261 ccaagagatg tccacaccta ggatgtcccg cggtgggtgg ggggcccgag agacgggcag
    16321 gccgggggca ggcctggcca tgcggggccg aaccgggcac tgcccagcgt gggcgcgggg
    16381 gccacggcgc gcgcccccag cccccgggcc cagcacccca aggcggccaa cgccaaaact
    16441 ctccctcctc ctcttcctca atctcgctct cgctcttttt ttttttcgca aaaggagggg
    16501 agagggggta aaaaaatgct gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc
    16561 aatcagcgtg cgccgttccg aaagttgcct tttatggctc gagcggccgc ggcggcgccc
    16621 tataaaaccc agcggcgcga cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac
    16681 agagcctcgc ctttgccgat cctctagagt cgagatccgc cgccaccatg attgaacaag
    16741 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    16801 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    16861 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
    16921 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    16981 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    17041 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    17101 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    17161 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    17221 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
    17281 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    17341 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    17401 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    17461 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    17521 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    17581 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    17641 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccccgga
    17701 tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag aggttttact
    17761 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    17821 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    17881 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    17941 tgtatcttat catgtctgga tccgggggta ccgcgtcgag aagttcctat tccgaagttc
    18001 ctattctcta gaaagtatag gaacttcgtc gagataactt cgtatagcat acattatacg
    18061 aagttatgtc gagatatcta gacccagctt tcttgtacaa agtggttgat atctctatag
    18121 tcgcagtagg cgggcgactc ttgataaatt gctttttcct tctgtttcca ctttgcctcc
    18181 tcttccgctg tgtaacccat ctcctctcct ttatcccatc tcctcccgtg ggagctaggt
    18241 tctcaatagc atgtgttacc atttaaccag catcctaaga agaagcctgg tctatctagg
    18301 agaggcctgg agtgtttcct gtgcttgtcc gggggtccag cacagtgctc cagacaggga
    18361 gaacgcaggt agaggaacag gactctgccc tacaaagatg tgagcagtga agagagtcat
    18421 ggcccagagc agaggcccct cccactcact ctgccggctt gctttgcagc tggctgtgga
    18481 agccctggag caggagaagc agggtctaca gagtcagatc gctcagatcc tggaaggtgg
    18541 gcagcaactg gcacacctca agatgtccct tagtctggaa gtggctacat acaggtacac
    18601 agtactgact gtctccttgg ctgaagggcc ccgaaacttg accctgtaaa actgttctat
    18661 ttacgtgtat ttgtgtatag gccccatgtg tccaggtgtc ttaagagccc agaagacact
    18721 gggtcgagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt
    18781 atggtctgag ctcgccatca gttcatgtga gcagctccac actggtgctg tgagccacac
    18841 ccctgttctc tgcaagggca gcaagcactc ttaaccactg agccatctgt ccagccctca
    18901 gatccactta aaacttttta ttacatgtat ttatgtaatg tttttgtctg tgatggaggc
    18961 ttatgagtcc cagagatcaa agaggttgtc tggcttggca gcttggtagg cataaacttg
    19021 agcccacctg ctcccaggtt cgggactaga agagatggtt atgcttaaag agacaactca
    19081 gtcttcaggg aggagaccag agggtacagt cacctgctct cgagttcttt gaaaggcagg
    19141 caatctccag tgtctatgcc agatcccagg gacacctggg cggtctcagg aaagagacct
    19201 tgcaggattg cctggtcgtg ctcaccggtc ccctagcccg ggcctcagct gtgtctgggg
    19261 taagagctgg gcgcctagcc ttctgccttc ctttcaagcc ctcgtgtcac tggccctgcc
    19321 cagctcctgt tgcccctttc ttggctgcct caggaaggct gagtggaaca gctgtgggta
    19381 gtttgtgtcc agcctctgtc tgtcagaaga ggtcccggct ggagtcccac tgttgagggc
    19441 tgaggatatc ccgccagagt atggggccag gacaggatgt cgcccttctc cagaatccac
    19501 tggcaaagag gcaagaccag agacagatct aggagaggag ctggagggta gagtccagag
    19561 aagggaagcc agtgagtgtc taggaaggac tgaatgcaca aggcaggaca ctatgaggac
    19621 ggaggaaagg ctattttgag aaccagatgt gctcagaggc catgaatgga aacaagggac
    19681 tagctccgaa tcccttgtga actgattccc ctcatctcct cccatcagct gtgaacctaa
    19741 agacctctgg cctgaaggag gatttgtgat cctaagatga gggccttcgc cccagtcagt
    19801 cttctgaggc gaggggaagg gtccaggcag ctctgaggaa tgtaagcact ggtgtttgcg
    19861 gtctgaaaag gatttggaga aggagagctg aattcatttg cttttgtctg tcaccagctc
    19921 tgggggccta ggagagagcc atcccatggg aacagcctga gaattcccac ttcccctgag
    19981 gagcccctcc ttctcaggcc ctccagatgg tagtgtggac aaaaggcaat aattagcatg
    20041 agaatcggcc tccctctccg aggatgaggt catcggcctt ggccttgggt ggggaggcgg
    20101 agactgatct gaggagtctg atcgaagtgt tagcaattca cttggccctg cttccgaatg
    20161 tgggaatctg cgtgtggggt ctccctgagt ctcaaatatg ggttcaccaa gtatatatct
    20221 gtgggtatat gactgtgtgg ctttcatacg acaatggtca caataaagag tgatcctgca
    20281 gtggcaggac atgcaacctc agctgggcct gaccccatcg cctcccctcc cccccaggac
    20341 tctgctggag gctgagaact ctcgcttgca gacacctgga agaagttccc aggcttctct
    20401 tggctttcct ggtaagaggc ggagccaact gctcctggac gctctccttc cacatcttcc
    20461 ctaagcagca tcagtccaag tcttcactcc ctggtcccca cagtactgat gtccccagtc
    20521 taagcagtgg gggtcggata aaatggctga aatgagggct gctctgggtg aagtcaggac
    20581 atacttacag tgtttctttt cttttttaag atttatttat tttatgtata tgagtatact
    20641 atagctgtac agatggttgt gaaccttcgt gtgattgttg ggaattgaat ttaggacctc
    20701 tgctggctcc agtctgccct gctcgctcag tccctatgca ctctggccca aagatttatt
    20761 tattattata aataagtaca ctgttgctgt cttcagacat actagaagaa ggcgtgagat
    20821 ctcattacgg gtggttgtga gccaccatat ggttgctggg atttgaactc aggaccttcg
    20881 gaagaacagt cagtgccctt acccgctgag ccatctcgcc agcccactta cagtgtttct
    20941 tactctgctt ttccagaccc caagctgaag ctgcatttcc ttgggatacc agaggaccag
    21001 cacctgggat ctgtgctccc tgtcctcagc ccaacatcct tctcttcccc cttgcctaat
    21061 acccttgaga ctcctgtgac agcctttctg aagacacagg aattccttaa ggccagaacc
    21121 cccaccttgg ccagcactcc catcccacct atgtctgagg ctccctatcc taaaaatgca
    21181 gaggtcagag cccaggatgt cccccattcc ctgctccagg gtgggaggca acaggctcca
    21241 gagcctcttt gggctgaggc cacagtgccc agttctactg gtgtcctccc agagctggag
    21301 gagcctgggg gcgagcagcc ggaccacttc cctgatgatc caacctcctt agccccaccc
    21361 ctcaaccctc accactctat tttagaggct aaagatagag aatccagtga gtctagagtt
    21421 tctagcatat tccaggaaga agaagggcaa atctgggaac ttgtaaagaa agaagcagcc
    21481 acagaggtaa aagtagaaaa cagcttagca caggaaatac aagaaagtgg tctggacaca
    21541 gaagaaatcc aggattccca gggacctttg cagatggaaa ccctggaggc tctaggagat
    21601 gagccactga tgtctctgaa aacccagaac catgagaccc caggaaagga gaattgcaat
    21661 tcatctatag aagagaactc ggggacagta aaaagcccag aaaaagaaaa acaaacacca
    21721 ctgaagtctt tagaagaaaa gaatgtagag gcagagaaaa ctctagaaaa tggggttctt
    21781 gaactatcta aacctttagg agaagaagaa ccaagaatgg aggatcaaga attaatgtct
    21841 cctgaacaca cactagagac agtttcattt ctaggaaagg aaaatcagga agtagtgagg
    21901 tcttcagaag aacagaactt agaatcattg ataactttta aagaggagag ccaataccca
    21961 ctgggaggtc cagaagccga ggaccagatg cttgaaagac tggtagagaa agaggatcag
    22021 aggttcccaa ggtctccaga agaagaccag caggcgttta gacctctgga gaaagagaat
    22081 caggagccac taagatttga agaagcagag gaccaggtgc ttgagagact gatagaaaag
    22141 gaaaggcagg agtccctgaa gtctccagaa gaagaggacc agcaggcatt tagacttctg
    22201 gagaaagaga atcaagaacc actaaggttt gaagacgcag aggaccaggt gcttgagaga
    22261 ctgatagaaa aggaaagaca ggagtccctg aagtctccag aagaagagga ccagcaggca
    22321 tttagacttc tggagaaaga gaatcaagaa ccactaaggt ttgaagaagc agaggaccag
    22381 gtgcttgaga gactggtaga aaaggaaagt caggagtccc tgaagtctcc agaagaggag
    22441 gaccagagga ctgggaagcc tctagaaaaa gaaaatcagg aatctctgag gtctcttgat
    22501 gaaaaccagg agacaattgt actgctagaa agcaagaacc agaggccact gagatctcta
    22561 gaagtagaag aggaggagca gagaattgtg aaacctctag aaaaagtgag ccaggtctcc
    22621 ctcgaatctc tcgaaaaaga gaatgtgcag tcaccaaggt atctggaaga agatgaccac
    22681 atgattaaga gcctgctaga agacaagact catgagatcc tgggatctct tgaagataga
    22741 aatggggaga actttatacc acctgaaaat gagacccagg gt
//