LOCUS       KO-first_condition_ready_70740_MGI:101783        22953 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Plk4 targeting project(s): 70740
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 119142
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            9613..9877
                     /db_xref="ENSEMBL:ENSMUSE00000365127"
                     /rank="1"
                     /note="ENSMUSE00000365127"
     exon            10594..10689
                     /db_xref="ENSEMBL:ENSMUSE00001275698"
                     /rank="2"
                     /note="ENSMUSE00001275698"
     misc_feature    5434..11008
                     /note="5 arm"
     misc_feature    11036..11060
                     /note="B1 Gateway"
     misc_feature    11073..11120
                     /note="Frt"
     intron          11121..11995
                     /note="mouse En2 intron"
     misc_feature    11993..11995
                     /note="Splice Acceptor"
     exon            11996..12168
                     /note="mouse En2 exon"
     misc_feature    12180..12773
                     /note="ECMV IRES"
     gene            12774..15834
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15873..16116
                     /note="SV40 polyadenylation site"
     misc_feature    16120..16153
                     /note="loxP"
     promoter        16180..16699
                     /note="human beta actin promoter"
     gene            16725..17519
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17719..17960
                     /note="SV40 polyadenylation site"
     misc_feature    17977..18024
                     /note="Frt"
     misc_feature    18031..18064
                     /note="loxP"
     misc_feature    12010..12087
                     /note="FCHK std_gpr"
     misc_feature    18080..18100
                     /note="B2 Gateway"
     misc_feature    11009..18130
                     /note="Synthetic Cassette"
     exon            18476..18571
                     /db_xref="ENSEMBL:ENSMUSE00001260863"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001260863"
     exon            18649..18763
                     /db_xref="ENSEMBL:ENSMUSE00001241115"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001241115"
     misc_feature    18131..18916
                     /note="Critical Region"
     misc_feature    18940..18973
                     /note="Downstream LoxP"
     misc_feature    18917..18996
                     /note="synthetic loxP region"
     exon            21671..22583
                     /db_xref="ENSEMBL:ENSMUSE00001249642"
                     /rank="5"
                     /note="ENSMUSE00001249642"
     misc_feature    18997..22953
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggaacatgaa cagacataag aacggcttcc
     5461 aatgttaatt gataatctat aaatatcatt ttggaatttg actggttcac aaatatatcc
     5521 acaaaatgtt tgcatatgga ataatagaag catataaaac attgtatgta aattgcatct
     5581 tcatactagc aaacaagtta acaaatgata ataaatgcaa ttcagttttg gatgttggtc
     5641 cacattgtca agaagtcatg taataataat aaatacatat tctacctgct acttgaacca
     5701 tgagttttgc acaccagagg tttaatgttt aagtgttgga ctttactcac tgtatgaaac
     5761 actgatgaca agtcaccaaa tcttcttaaa taaggaactt gcaaaacaat ggggctttta
     5821 agtaattttc agacacttga ttctgaagag tcatatggtc atttttgagt tttagatgtg
     5881 gtaatggaca aaagcttgag agtgaagtaa agctttggaa cttctcaggt tgagaagaat
     5941 ttctgcccag gtcctctgtt taggacctgc tgctcctgga gctggtgagg tggctcaacc
     6001 aacttgctct gcaaggctga ggatctgagt ctacgcccta gatctcatga tggaaggagt
     6061 gataccaact gtacaaaatt gtcctgactg tgacatgcat gtgcatgcaa acatgcatgt
     6121 aaaataaaaa ggaactgctg ttcctggggc tgggggatgt ctcattaaaa tccaagtgca
     6181 agtgtgtgga tccagagcac ccacttaaaa caaagcaaaa ctagggttag tggcactgag
     6241 gaaatggact catgagatcc ctagatccct acaggtttag tgagagacca catctcaaag
     6301 cccgctgtgg tagtgtacgt ctttaatccc agtatttggg aagcagaggc aggacgaatt
     6361 ctgtgtctca agatcagttc cggatacaaa agtaactgct gttcctaata aaatgtgtgt
     6421 gtgtgtgtgt gtgtgtgtgt gttcctgata aaggaacata ctaataaaaa taattgctgt
     6481 tcctgataaa aaaataaata taatatatgc aatattaatc ttctgggggg attaagatag
     6541 cttgtaaaaa aaggatatgt atcctcaatc tagaaaaaca tttatgtcat acactttaaa
     6601 tttccaaatt atctcagcca cagagtggtg gtagtggtgc actcctttaa tcccagcact
     6661 ccagaggcag agacagatct cttcacctgc tttacagaac aagttccatg acagccagag
     6721 ctacacagag aaacccagtc tcaaagtctc aatgaaatgc acatattcta aaagtcttag
     6781 atggttaagc tacagtcagc tgttcatgag ttgaaaattt gaaagggttt cttaaaaaaa
     6841 aaaaaaagct ctctagttag gtaatatgag gaaggagaat gttttagagg taagaaagga
     6901 ctgttataag catgtcttta ctaacttatc tacaaaattt ccccctttgg ataaagacat
     6961 agattcaaga tggttcacac agcccggaag tctcacaggc agcatgatgt cagcagagtg
     7021 agcacacttt ccgcagtgat gctttggcca cctgtttaat ctcactgtca acaggataaa
     7081 actgatttcc ttagtgatca gagatgacaa gaatataaaa ttaatagaag ataacataaa
     7141 ctaaattgta atccattgct aaaagaagaa aacattgaaa tcacattaaa aagtctagta
     7201 gccaggtgag tactagtgaa acagagggag ggtgtccatt gcaagtaagt acaaagtcag
     7261 gtctacagag accttgtctt agtaagtaaa taaaaaccta aacactgata gctactttaa
     7321 tgacatcata tttgccagtt tgaagagtat ttgtggctta tagaagatcc aaaaaaaaaa
     7381 aagatgtagt agagaaaaag ttatgctaag aacatacaaa aaggtaagtg aaaggctaag
     7441 aaaaaatgat tctacaggtt aaaaaaatgt ttctgtaaat atgagtagta taaattaaaa
     7501 ctttagagca taaagagggt attctttgat tatagagaat tcattagaaa tatatatgag
     7561 ctctgacaca ggtctgagaa catagactgc agctgacaat gaagtgataa cacaaggcaa
     7621 atatataaag caacaaaaag taacgagggc atggatttat atcaacagac tattaatggg
     7681 ctctgataaa ctttaaatac atgtgaaata tttacaaaaa ctctgaggcc acaaaataaa
     7741 gcccaggaat accaaagaca tctgcaagac ttttaagcaa cagtctaaat catctatgaa
     7801 actagatcgg gtagcacaca tctaaaatct agctctggaa ttcaaggcca gcctgaggtg
     7861 catagcatga ccctgagtca taataatctt atttgcctat taagacactg caagggaaat
     7921 tctaatatgc cagtgtgctt gaaattagaa ttctagaatg ttaaatcatg ttgaaaattg
     7981 agagtgcttc tatgaatgta aacattcctt aaactacaaa tgaatccatt aaattgcagg
     8041 tttttgtttt tgatgtgcaa tcattcctgt gtgtacgtaa attacgtgtt cattataaag
     8101 tatcttcaca caagctccat ccggcaggca cggtgctttg ttttcaaaca gctttgagaa
     8161 agttctgtac atgagatcag cttttttgtt tgtagatttt atttttataa cgtgagttta
     8221 tgtgagtata ggtttgtgta catgagtgct atgtcgatag gagccagaag gtggcatcag
     8281 agcccctgga cgtggataac aggaggctag ctgacagcat tgagtactga taggcaaata
     8341 tggctctctc ccacagcagc taggtctcac tcactatgtc ggtatctcct acccctgaga
     8401 tgtgcacagt gtctgaaaag catgaaactt gtgaagtgat gccttgcttg caggataacg
     8461 tgttcattta aatatgtgtg gaggttggcc ttaattgtct caaggatact gcagccaaag
     8521 acaaaaaggt cctaaatgag gacctcactc agcatagaac cttttaaaag tgcccgctag
     8581 cctattcctc ttattcctaa ctataaattg cccttggaga cacaggcccc cactaagacg
     8641 actcatcttt gttgatagac cccctgacca cccaggtgcc atcatctctg atttgctgtc
     8701 tttccaaatg aatctttcct tgcctcactt cttgtcctgg aatcactggc atttggggat
     8761 agcaatctag acctggattg gaacactatt aggagttgag ataaaacaca gacacagaga
     8821 gaacacacta ttgaggatct tcagaaaaca agtcactact ttggatcttt ttgcatctga
     8881 ttaaaaaatc tcaaaacaat taggctaaaa agcaaacccc tgtcggatta tttactagca
     8941 actaatgtaa agatccatac cactactctt tcttcaaaac atttttagag gaaaatgact
     9001 tagcctaggt cactcacata tggctcaaaa taaatttgta agattggtag cccaggttag
     9061 cctggaactc cttagagtcc aagaatggac tgactcaacc ccagtgcgtc gctgtgtcca
     9121 acctttaata tctgtccggg cagcgggttg ctgggaaaga agaaactaga acccaaggac
     9181 atgtccacgc ttggaaagca ttttaccact taggtacatc cccaggcttt ttttacttta
     9241 gagtcggtgt tttccctata cagttcatct taaagcacca cacattaata gctgtaaaaa
     9301 aggaaaatgg cacaacggtg tacatggtgg gctggcgggt tcggggctgt tctaaggaaa
     9361 gtaatggact tttcccccta gttctcttta ggccaaacct acacacttat ttaaaagact
     9421 ttctgcggga agccaaagcc ctattatcct tcaggtcaaa acgtaaccta ttattagcgt
     9481 ccgcgctacg gtcagtcgta cactgaccgc tttccgtcaa acttaaaacc cacttccggc
     9541 ctagagcgag ggaaagcccc tgctgcgcat gcgtgtcggt ctccgcctct aagaagtgga
     9601 gagtgtgctg acgtcaccgc caggctggct cgttcggaga ccggcgggaa tttttcaaaa
     9661 tgggagctcc ggggcgccgc ccaggcctcg ggaggtaccg ggggtacctt tcggtggcgt
     9721 tggcggcgtc gccagcggcg gcgtagagaa ggcgtcctga tgggcgccaa gacctgctgg
     9781 cttctcggag cgctgcctcg gagggactgc gagaaggccg agccccgggc gccggcggct
     9841 cgggaacatg gcggcgtgca tcggggagag gatcgaggtg aggccggcgg gcgcggagct
     9901 gagggaaggt ccgatggagg cggactctgg tattccggga cccagtgagc tgaggtgaac
     9961 cgagcgcagt gctgagggga ggtccattgt ggctcggcac aaacacacgg gagaccagac
    10021 gggttagcgg gctgaggctg aggcccagtc tggctcaggc gctcactggt gctcagtgac
    10081 actagtaaag cggggatgag gcgggcaggc gcggtggcgg tggcggggct gatcgccgag
    10141 ggacaggcga agcgacccgc cccctggagc cccgcccgga ggggagcgcc ttgtaccccg
    10201 caggtgtgtc tccagctgcg ggccccgccc ggtcctggtc ccttggcgtc gggtccgcac
    10261 tcccgcctcc aaagccgcgc tccgcttgcc ccctccccgg ggatggcggt taccgtggcg
    10321 ggagctgcgg cgggaacctg gtgggccaga ggagggaccc gagctaacca gctcattaga
    10381 gtccttagcc tccatcaggg agggagctca gaaaatctct cagagcgtca agatgccttc
    10441 caccccagac acctgcatca tcgaggtgtg gcagctgctt gaaccatgtc tccttttctc
    10501 tttggcccat ttcacatccc atcccctacc caactttctt actttctaga tctgcgattc
    10561 ctcacgtgta atccttaatt ttttttttaa aaggacttta aggttggaaa tctactcggt
    10621 aaaggatcat ttgctggtgt ctacagagct gagtccatac acactggttt ggaagttgca
    10681 atcaaaatgg taagaatgta ttagttattg tttcttcagt atttttgcaa ggtactagag
    10741 agcaatgaag gcatgctatt taaaatgtcc cactcattat ctgctaaaga ctgcaccttg
    10801 ccaccctgcc aaatgtgtgc atttgtaata attgcataat acattgcata accaaaagac
    10861 agctgttaag tacagcatct gaatttaagg acatttaaga gactcttaac cttgcttaag
    10921 tgaagtttta tactttacta gggttcagga gaccagtaca ttttgttgct atgaatactt
    10981 aaggtaatga gaagtcagca atttataaaa ggcgcataac gataccacga tatcaacaag
    11041 tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct
    11101 ctagaaagta taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca
    11161 ctgccaacta tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct
    11221 gttgggaggg tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag
    11281 gtggtcccaa ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc
    11341 ccctatccca taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag
    11401 agactcagtg aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca
    11461 ccaggcctca gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt
    11521 gtgggttaca taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc
    11581 agtcttctcc atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa
    11641 agataagggg agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc
    11701 cgctagcttt tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc
    11761 catctcattt tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc
    11821 ctcaagttta tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc
    11881 ccccccccac ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa
    11941 gtagctagac tccagcaacc agtaacctct gccctttctc ctccatgaca accaggtccc
    12001 aggtcccgaa aaccaaagaa gaagaaccct aacaaagagg acaagcggcc tcgcacagcc
    12061 ttcactgctg agcagctcca gaggctcaag gctgagtttc agaccaacag gtacctgaca
    12121 gagcagcggc gccagagtct ggcacaggag ctcggtaccc ggaagatctg gactctagag
    12181 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
    12241 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
    12301 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
    12361 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
    12421 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
    12481 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
    12541 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
    12601 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
    12661 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
    12721 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca accatggaag
    12781 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    12841 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    12901 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
    12961 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
    13021 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
    13081 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
    13141 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
    13201 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
    13261 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
    13321 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
    13381 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
    13441 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
    13501 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
    13561 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
    13621 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
    13681 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
    13741 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
    13801 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
    13861 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
    13921 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
    13981 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
    14041 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
    14101 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
    14161 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
    14221 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
    14281 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
    14341 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
    14401 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
    14461 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
    14521 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
    14581 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
    14641 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
    14701 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
    14761 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
    14821 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
    14881 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
    14941 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt
    15001 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
    15061 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg
    15121 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg
    15181 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg
    15241 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga
    15301 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
    15361 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc
    15421 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
    15481 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
    15541 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
    15601 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac
    15661 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
    15721 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
    15781 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
    15841 cgggcagggg ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
    15901 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    15961 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    16021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    16081 ccaaactcat caatgtatct tatcatgtct ggatccggaa taacttcgta tagcatacat
    16141 tatacgaagt tatgtttaaa cggcgcgccc cggaattcgc cttctgcagg agcgtacaga
    16201 acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
    16261 agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc
    16321 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg cgcgggggcc
    16381 acggcgcgcg cccccagccc ccgggcccag caccccaagg cggccaacgc caaaactctc
    16441 cctcctcctc ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga
    16501 gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat
    16561 cagcgtgcgc cgttccgaaa gttgcctttt atggctcgag cggccgcggc ggcgccctat
    16621 aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga
    16681 gcctcgcctt tgccgatcct ctagagtcga gatccgccgc caccatgatt gaacaagatg
    16741 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
    16801 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
    16861 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
    16921 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
    16981 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
    17041 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
    17101 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
    17161 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
    17221 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
    17281 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
    17341 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
    17401 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
    17461 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
    17521 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
    17581 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    17641 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccccggatct
    17701 aagctctaga taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc
    17761 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    17821 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    17881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    17941 atcttatcat gtctggatcc gggggtaccg cgtcgagaag ttcctattcc gaagttccta
    18001 ttctctagaa agtataggaa cttcgtcgag ataacttcgt atagcataca ttatacgaag
    18061 ttatgtcgag atatctagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    18121 cagtaggcgg aacggtccaa gaataccatt atggtcagcc tgtgatccca gttctgaaga
    18181 gttgggaaaa agaaaatcct aactccaggg ccaatctctg ctatcacata aatgcactgg
    18241 acatactggg aatgtactct gttaggtcga gggcagagca cagataggag taaactgact
    18301 tcaaagacta tattaaaaaa aaaagacttc ttacaattat aggttgttgt ttgagctttt
    18361 gtctgggctt aggggtatag ctaggtggta gagtgctttt ctctagcatg cacagggctc
    18421 tagatcaagc cacaaccata aaaatcaaca aataaatctg ccctcttatt tttagataga
    18481 taagaaagcc atgtacaaag ctggaatggt acagagagtc caaaatgagg tgaaaataca
    18541 ttgccagttg aaacacccct ctgtcttgga ggtaagatac cagatgtatt aaagtagtta
    18601 aggaaagctg gatgttcgta tagttgacgg cacccttttc tgttttagct ctataattac
    18661 tttgaagata acaattatgt ctacctggta ttggaaatgt gccacaatgg agaaatgaac
    18721 agatatctga agaacagaat gaagcctttc tcagaaaggg aaggtgggtg tcttttgggg
    18781 gttggggtgt ggaatttgtc tctctctgtt ctgtttggta aaacttggct tttcaatgtg
    18841 tggttttgaa aaatgtggta tttttataac atttgtgtaa ttttgttttg ttgatgcaag
    18901 gactctggct gtcctagaga tggcgcaacg caattaatga taacttcgta tagcatacat
    18961 tatacgaagt tatggtctga gctcgccatc agttcatttt ttgcatgtat tgagtcttta
    19021 cctgcttgtg tgtctgtaca ctgtatgtgt gcctggtgct cagggagttc agaggaagaa
    19081 attagatccc ctggaacctg attgtgaggt accatgtgag tgttgtgaat ccaggtcctc
    19141 tgggtggtga gctaggactc ttatccactg ggccatcctt tcagcctcaa caatttcttt
    19201 tctgttttta aggcatgtgt ataagtgtgc ctctgtctat gagtatgtgc atgtagtgta
    19261 ggtaggggtc cctgcccttg gagggttaac atggatactg ggaatagagt ttgggtcctt
    19321 tgcaagaaca gtatgtatct ctttatcagc tgtgtcatct ctccaaccac agataaaact
    19381 attttaaatt taaaaaggag aactccccac aaaactggca tttataaatc aagtattaca
    19441 aaagtagaga gatttgtaag ccttctgttc tctaaaaaca aatttaaatt aaattattag
    19501 taacagttta tagaaaatcc tttcaaatct ggaaaataac ttagaatgtc tttgactaac
    19561 atttccaaat aaacattatg aacatttact atatatgaaa ggtctgaaac gcagagaact
    19621 atgcttatta cttattgctt attagcttat tagtcaactt ctgaaaggat ggagtgattc
    19681 agactcccca ggtaacatat ctttgcaatg cagattttta aatgcacata gcaacaaagc
    19741 tttttttcac acataataat attttttatg aaatacaaaa tgaatttcaa tttgtacccc
    19801 cattttctta aatcagataa gctccatatg ttgttaaact actgttttgg tttggttttt
    19861 tttctcttcc aaactacaac taatactgca gatagagcta aggaatatgg atgctgatag
    19921 gtttttggtt ttgtttttac tatctgttat tcatttgtat cttgtgtgtt atggtcggag
    19981 gttagctcta gggagttggc catctctttt tatcaggtgg gctctagata tctaactcag
    20041 gttgtcaaac acctacaaac accttttcct gcttaactat cacactggcc tgacagagca
    20101 ttttatacat ttaaatctgt tggacagtgg taacaaatac taaattgtga aacattgata
    20161 acttttctct caagttccat ttttaatagt taattctgag gaaataatac aaactggaga
    20221 ggatgggttg catacatata gatgttggtt attgtatttt tcaaaatgct ttataatttt
    20281 ttataaacat tttactgaga caatatttat aaagaaaaaa attatgttag accgaactaa
    20341 acttaaaaac ttatatataa ccaaagatgc actccccttc caccaacaaa gtttaagaca
    20401 gagatgataa gaaaatatct gcaaacaaaa tgtatgactg atgaggctgg ggctgtagtt
    20461 cagtgggtat gtttaattca gcacatatgc acaaagccca ggctccctgt tttccgctga
    20521 caaatttgtc tgtagtgcat ttaactaaaa aactcaggct gaaaaattaa aggtgtactt
    20581 aaaaggggaa ggataatcaa ataagaaact agaagatatg agcagactat ttactaagga
    20641 gaaagctcaa atagctaata aacatgaaag acgtgtagac tcagtagtgg tcatgagggt
    20701 cctaaccaaa gtaatgaagg accatttccg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
    20761 tgtgtgtgtg agagagagag agagagagag agagagagag agagagagag agagagagag
    20821 agagaaactc actataacac aaaaagtaag aacaagaatg ttatattttt gtgaaatctt
    20881 gaaacatcag aaattataca aatgtccaat ggcagttaag taaaattttt taaaaatctg
    20941 tgatattgtt atagaaataa ctttgaaata tgctaaagaa ggaaaagtaa tgaaactatt
    21001 ttgataccat ttgttttaat attttaaatg cattatgtct gtagctttat atgtagttat
    21061 gcagaaacat ataagaagta tatcaaatgt ttacagtgga ggctgtgtgt taagttgcca
    21121 tgcaagtgtg agaactgggg tttggacccc agcactcata gaaatgcttg ggtggatgtg
    21181 acaacctgct tattgtactt ggtactcagg aactagaaat aagattcccc caggcgcttg
    21241 ttagctggaa taagtgagtt ctggatttaa gagacctgcc tcagtctgta agatggaaag
    21301 ctttgggaca gagtttggga agcattaaag aaatatactt tttgttttac aagtaatata
    21361 tttattgttt gtacaattta aattcttgtg tttatctagt tacctagtta ctctaaacac
    21421 tttcaactag tataaagttt tgcttctgtt agctcataag taagaaaaag caaagattaa
    21481 aaggagtgtc tagttttctt gaggggaatt agatagcatt atattgatag aatggtgttt
    21541 tagttgataa atctaacctg caagctagga ctttaatact ctggcgtgta tactagattt
    21601 tatcgtgtat tctaaacaat aaatttccca gatttgaatt gttaagctct ctctttttct
    21661 atcttcacag ctaggcactt catgcaccag attatcacag gaatgttata tcttcattct
    21721 catggcatat tgcaccggga cctcacactc tctaacatct tacttacgcg gaatatgaac
    21781 ataaaaattg ctgactttgg actagcaacg cagttgaata tgccacatga aaagcactat
    21841 acactctgtg ggactcctaa ttatatttca ccagaaattg caactcgaag tgcacatgga
    21901 cttgaatctg atatttggtc attgggctgt atgttttata cgttacttat tggaagacca
    21961 ccttttgaca ctgacacagt caagaacaca ttgaacaaag tagtcctggc agattatgaa
    22021 atgccagcct ttttgtcacg agaggcccag gaccttatcc accagttact tcgtagaaac
    22081 cctgcagatc ggttaagtct gtcttctgtg ttggaccatc ctttcatgtc acgaaatcct
    22141 tcaccaaaga gtaaagacgt agggactgta gaggactcaa tggatagtgg gcatgctaca
    22201 ctttccacaa caattacagc ctcttctggt accagtttga gtggcagcct acttgacaga
    22261 agacttttgg ttggtcaacc acttccaaat aaaattactg tatttcaaaa aaataaaaat
    22321 tcaagtgact tttcttcagg agatggaagt aatttttgta ctcaatgggg aaatccagaa
    22381 caagaagcta atagtagggg acgggggaga gtgattgaag atgcagaaga gaggccgcat
    22441 tctcgatacc tgcgcagagc tcattcctct gatagagcca gcccctctaa tcagtctcga
    22501 gcaaaaacat actcagtaga acgttgtcac tcagtagaaa tgctttcaaa gcctagaaga
    22561 tcactggatg aaaatcaaca caggtattca cccaccaaaa gcaatgtcaa tgttttaact
    22621 tcattaaaca ccaaacagcc aatagttaag gatcttttga aagaccgtat aatgactgag
    22681 cagtataagg ataatctttt aaacttattg aacaagtttg atcgctaaag gactaatagc
    22741 tgcatgtaga gggaagctgg ggctgagtga ggaatagctg tgatactgac tgccttctga
    22801 tatgaacagt agttttattg cctaattgta gttttgtgtg tgatcttctt aatgtttgag
    22861 ttacacactt ataaaaggat agtaggcata cagtggtgcc attatagtac catggcattt
    22921 tggtattgca gttttagaga tgcttataca aat
//